{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T06:25:09Z","timestamp":1787034309745,"version":"build-2736575974"},"reference-count":59,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T00:00:00Z","timestamp":1787011200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100020595","name":"National Science and Technology Council","doi-asserted-by":"publisher","award":["112\u20132311-B-006\u2013006-MY3"],"award-info":[{"award-number":["112\u20132311-B-006\u2013006-MY3"]}],"id":[{"id":"10.13039\/100020595","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100020595","name":"National Science and Technology Council","doi-asserted-by":"publisher","award":["111\u20132311-B-006\u2013009"],"award-info":[{"award-number":["111\u20132311-B-006\u2013009"]}],"id":[{"id":"10.13039\/100020595","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100020595","name":"National Science and Technology Council","doi-asserted-by":"publisher","award":["107\u20132311-B-006\u2013002 -MY3"],"award-info":[{"award-number":["107\u20132311-B-006\u2013002 -MY3"]}],"id":[{"id":"10.13039\/100020595","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Cell Dev. Biol."],"abstract":"<jats:p>\n                    Steroid hormone signaling coordinates developmental transitions and tissue morphogenesis, yet how its activity is temporally tuned during collective cell migration remains poorly understood. Here, we identify the Hsp40 co-chaperone Droj2 as a novel regulator of ecdysone signaling during\n                    <jats:italic>Drosophila<\/jats:italic>\n                    border cell migration. Through a forward genetic modifier screen,\n                    <jats:italic>droj2<\/jats:italic>\n                    emerged as a potent enhancer of Taiman-mediated ecdysone signaling activity. Functional analyses revealed that loss of\n                    <jats:italic>droj2<\/jats:italic>\n                    disrupted border cell detachment and directional migration, with earlier depletion causing progressively more severe defects, indicating a critical temporal requirement during migration. Mechanistically, Droj2 depletion selectively triggered precocious downregulation of the nuclear hormone receptor isoform EcRA and premature activation of the ecdysone signaling reporter,\n                    <jats:italic>EcRE-lacZ<\/jats:italic>\n                    , during oogenesis, whereas EcRB1 and Taiman expression remained unchanged. In addition to the ovary, Droj2 was also required for proper EcRA nuclear accumulation in larval salivary glands, suggesting a conserved role in coordinating developmental steroid hormone responses across tissues. Together, our findings identify Droj2 as a spatiotemporal regulator of EcRA dynamics that couples chaperone activity to steroid hormone responsiveness during collective migration. This study uncovers a previously unrecognized mechanism linking molecular chaperones with nuclear receptor regulation and developmental timing during tissue morphogenesis.\n                  <\/jats:p>","DOI":"10.3389\/fcell.2026.1881376","type":"journal-article","created":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T05:36:36Z","timestamp":1787031396000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Hsp40 co-chaperone Droj2 temporally regulates EcRA dynamics during collective cell migration"],"prefix":"10.3389","volume":"14","author":[{"given":"Chueh-Wen","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Biotechnology and Bioindustry Sciences, National Cheng Kung University","place":["Tainan City, Taiwan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui-Ying","family":"Yu","sequence":"additional","affiliation":[{"name":"Department of Biotechnology and Bioindustry Sciences, National Cheng Kung University","place":["Tainan City, Taiwan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kuan-Lin","family":"Lai","sequence":"additional","affiliation":[{"name":"Department of Biotechnology and Bioindustry Sciences, National Cheng Kung University","place":["Tainan City, Taiwan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicholas Lee Kong","family":"Yau","sequence":"additional","affiliation":[{"name":"Department of Biotechnology and Bioindustry Sciences, National Cheng Kung University","place":["Tainan City, Taiwan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu-Chiuan","family":"Chang","sequence":"additional","affiliation":[{"name":"Institute of Biomedical Sciences, National Sun Yat-sen University","place":["Kaohsiung, Taiwan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna C.-C.","family":"Jang","sequence":"additional","affiliation":[{"name":"Department of Biotechnology and Bioindustry Sciences, National Cheng Kung University","place":["Tainan City, Taiwan"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,8,18]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"2629","DOI":"10.1534\/g3.116.028951","article-title":"A genetic mosaic screen reveals ecdysone-responsive genes regulating drosophila oogenesis","volume":"6","author":"Ables","year":"2016","journal-title":"G3 (Bethesda)"},{"key":"B2","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.32943","article-title":"Jak-stat pathway induces drosophila follicle elongation by a gradient of apical contractility","volume":"7","author":"Alegot","year":"2018","journal-title":"Elife"},{"key":"B3","doi-asserted-by":"publisher","first-page":"965","DOI":"10.1126\/science.277.5328.965","article-title":"AIB1, a steroid receptor coactivator amplified in breast and ovarian cancer","volume":"277","author":"Anzick","year":"1997","journal-title":"Science"},{"key":"B4","doi-asserted-by":"publisher","first-page":"67","DOI":"10.1016\/S0092-8674(00)80624-8","article-title":"Molecular chaperones activate the drosophila ecdysone receptor, an RXR heterodimer","volume":"101","author":"Arbeitman","year":"2000","journal-title":"Cell."},{"key":"B5","doi-asserted-by":"publisher","first-page":"1047","DOI":"10.1016\/s0092-8674(00)00208-7","article-title":"Regulation of invasive cell behavior by taiman, a drosophila protein related to AIB1, a steroid receptor coactivator amplified in breast cancer","volume":"103","author":"Bai","year":"2000","journal-title":"Cell."},{"key":"B6","doi-asserted-by":"publisher","first-page":"35","DOI":"10.1186\/1471-213X-12-35","article-title":"The lack of autophagy triggers precocious activation of notch signaling during drosophila oogenesis","volume":"12","author":"Barth","year":"2012","journal-title":"BMC Dev. 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