{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T06:06:57Z","timestamp":1788934017450,"version":"build-2803163510"},"reference-count":47,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T00:00:00Z","timestamp":1788912000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82370680"],"award-info":[{"award-number":["82370680"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31771274"],"award-info":[{"award-number":["31771274"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32270364"],"award-info":[{"award-number":["32270364"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31270361"],"award-info":[{"award-number":["31270361"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82474138"],"award-info":[{"award-number":["82474138"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012226","name":"Fundamental Research Funds for the Central Universities","doi-asserted-by":"publisher","award":["CZZ23001"],"award-info":[{"award-number":["CZZ23001"]}],"id":[{"id":"10.13039\/501100012226","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012226","name":"Fundamental Research Funds for the Central Universities","doi-asserted-by":"publisher","award":["CZZ21004"],"award-info":[{"award-number":["CZZ21004"]}],"id":[{"id":"10.13039\/501100012226","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012226","name":"Fundamental Research Funds for the Central Universities","doi-asserted-by":"publisher","award":["CZZ24011"],"award-info":[{"award-number":["CZZ24011"]}],"id":[{"id":"10.13039\/501100012226","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012226","name":"Fundamental Research Funds for the Central Universities","doi-asserted-by":"publisher","award":["XTZ20021"],"award-info":[{"award-number":["XTZ20021"]}],"id":[{"id":"10.13039\/501100012226","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012226","name":"Fundamental Research Funds for the Central Universities","doi-asserted-by":"publisher","award":["CZY23021"],"award-info":[{"award-number":["CZY23021"]}],"id":[{"id":"10.13039\/501100012226","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Cell Dev. Biol."],"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      Progenitor cells generate multiple cells during development and are thought to be involved in tissue repair. The widespread distribution of Kidney-specific cadherin-positive (KSP\n                      <jats:sup>+<\/jats:sup>\n                      ) cells in the kidney suggests they play an important role in renal development. However, direct evidence demonstrating that KSP\n                      <jats:sup>+<\/jats:sup>\n                      cells function as progenitor cells contributing to renal development and repair is lacking.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      To obtain direct evidence for the differentiation and regenerative potential of KSP\n                      <jats:sup>+<\/jats:sup>\n                      cells, we performed\n                      <jats:italic>in vivo<\/jats:italic>\n                      lineage tracing using\n                      <jats:italic>Ksp-Cre<\/jats:italic>\n                      and\n                      <jats:italic>Rosa-LSL-tdTomato<\/jats:italic>\n                      (\n                      <jats:italic>RFP<\/jats:italic>\n                      <jats:sup>\n                        <jats:italic>f\/f<\/jats:italic>\n                      <\/jats:sup>\n                      ) transgenic mice, combined with thymidine analog (5-chloro-2\u2032-deoxyuridine, CldU) labeling in adult wild-type (WT) mice. Lineage tracing was analyzed from embryonic day 14.5 (E14.5) to adulthood by triple immunofluorescence staining for KSP, red fluorescent protein (RFP), and nephron segment-specific markers. Unilateral ureteral obstruction (UUO) was performed to investigate the regenerative potential of KSP\n                      <jats:sup>+<\/jats:sup>\n                      cells following renal injury. Proliferative capacity was assessed by CldU\/Ki67\/PCNA labeling from days 2\u201314 post-surgery. To explore the molecular mechanism potentially underlying KSP\n                      <jats:sup>+<\/jats:sup>\n                      cell-mediated repair, we analyzed Notch signaling pathway in UUO-subjected WT mice.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      The\n                      <jats:italic>Ksp-Cre: RFP<\/jats:italic>\n                      <jats:sup>\n                        <jats:italic>f\/f<\/jats:italic>\n                      <\/jats:sup>\n                      lineage-tracing model exhibited high efficiency and specificity, with RFP expression strictly confined to KSP\n                      <jats:sup>+<\/jats:sup>\n                      cells at E14.5. Lineage tracing revealed that embryonic KSP\n                      <jats:sup>\n                        <jats:italic>+<\/jats:italic>\n                      <\/jats:sup>\n                      cells function as progenitor cells, giving rise to cells in the formation of multiple nephron segments, including proximal tubules (PTs), loops of Henle (HLs), distal convoluted tubules (DCTs), and collecting ducts (CDs) during kidney development. Comparison of the differentiation capacity of KSP\n                      <jats:sup>+<\/jats:sup>\n                      cells during embryonic versus postnatal development indicated that KSP\n                      <jats:sup>+<\/jats:sup>\n                      cells preferentially contribute to postnatal kidney development. In adult kidneys under homeostatic conditions, KSP\n                      <jats:sup>\n                        <jats:italic>+<\/jats:italic>\n                      <\/jats:sup>\n                      cells showed limited proliferative potential. However, following UUO injury, KSP\n                      <jats:sup>+<\/jats:sup>\n                      cells exhibited markedly increased proliferation. Notably, the proliferative response of KSP\n                      <jats:sup>+<\/jats:sup>\n                      cells was closely correlated with Notch activation.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      Our lineage tracing data supports the KSP\n                      <jats:sup>+<\/jats:sup>\n                      lineage behaves as a multipotent progenitor population. These cells give rise to cells of multiple nephron segments and preferentially drive postnatal kidney development. In adult kidneys, KSP\n                      <jats:sup>+<\/jats:sup>\n                      cells are quiescent under homeostasis but become highly proliferative after renal injury, and this proliferative response is closely associated with activation of the Notch signaling pathway.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.3389\/fcell.2026.1893353","type":"journal-article","created":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T05:39:12Z","timestamp":1788932352000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Lineage tracing reveals KSP+ cells as multipotent progenitors driving postnatal renal development and injury repair via notch signaling"],"prefix":"10.3389","volume":"14","author":[{"given":"Xinghao","family":"Duan","sequence":"first","affiliation":[{"name":"College of Life Sciences, South-Central Minzu University","place":["Wuhan, Hubei, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fuyu","family":"Ji","sequence":"additional","affiliation":[{"name":"College of Life Sciences, South-Central Minzu University","place":["Wuhan, Hubei, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haoyu","family":"Zhu","sequence":"additional","affiliation":[{"name":"College of Life Sciences, South-Central Minzu University","place":["Wuhan, Hubei, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruoqi","family":"Hu","sequence":"additional","affiliation":[{"name":"College of Life Sciences, South-Central Minzu University","place":["Wuhan, Hubei, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Caiyue","family":"Li","sequence":"additional","affiliation":[{"name":"The Eighth Affiliated Hospital of Southern Medical University","place":["Foshan, Guangdong, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenbo","family":"Ma","sequence":"additional","affiliation":[{"name":"The Eighth Affiliated Hospital of Southern Medical University","place":["Foshan, Guangdong, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ping","family":"Zhao","sequence":"additional","affiliation":[{"name":"College of Life Sciences, South-Central Minzu University","place":["Wuhan, Hubei, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yongbo","family":"Peng","sequence":"additional","affiliation":[{"name":"College of Life Sciences, South-Central Minzu University","place":["Wuhan, Hubei, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinhua","family":"Shen","sequence":"additional","affiliation":[{"name":"College of Life Sciences, South-Central Minzu University","place":["Wuhan, Hubei, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guanghui","family":"Yu","sequence":"additional","affiliation":[{"name":"College of Life Sciences, South-Central Minzu University","place":["Wuhan, Hubei, China"]},{"name":"Hubei Provincial Key Laboratory for Protection and Application of Special Plants in Wuling Area of China, College of Life Sciences, South-Central Minzu University","place":["Wuhan, Hubei, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chao","family":"Gao","sequence":"additional","affiliation":[{"name":"College of Life Sciences, South-Central Minzu University","place":["Wuhan, Hubei, China"]},{"name":"The Eighth Affiliated Hospital of Southern Medical University","place":["Foshan, Guangdong, China"]},{"name":"Hubei Provincial Key Laboratory for Protection and Application of Special Plants in Wuling Area of China, College of Life Sciences, South-Central Minzu University","place":["Wuhan, Hubei, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,9,9]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"F839","DOI":"10.1152\/ajprenal.00128.2002","article-title":"Regulation of kidney-specific Ksp-cadherin gene promoter by hepatocyte nuclear factor-1beta","volume":"283","author":"Bai","year":"2002","journal-title":"Am. Journal Physiology Ren. Physiology"},{"key":"B2","doi-asserted-by":"publisher","DOI":"10.1002\/0471142956.cy0738s55","article-title":"Dual-pulse labeling using 5-ethynyl-2'-deoxyuridine (EdU) and 5-bromo-2'-deoxyuridine (BrdU) in flow cytometry","author":"Bradford","year":"2011","journal-title":"Curr. Protoc. Cytom."},{"key":"B3","doi-asserted-by":"publisher","first-page":"722","DOI":"10.1038\/nrm.2016.94","article-title":"Notch signalling in context","volume":"17","author":"Bray","year":"2016","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"B4","doi-asserted-by":"publisher","first-page":"522","DOI":"10.1038\/s41580-025-00835-2","article-title":"Modes of notch signalling in development and disease","volume":"26","author":"Bray","year":"2025","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"B5","doi-asserted-by":"publisher","first-page":"2022","DOI":"10.1681\/ASN.2016080840","article-title":"IL-36 signaling facilitates activation of the NLRP3 inflammasome and IL-23\/IL-17 axis in renal inflammation and fibrosis","volume":"28","author":"Chi","year":"2017","journal-title":"J. Am. Soc. Nephrol."},{"key":"B6","doi-asserted-by":"publisher","first-page":"264","DOI":"10.1038\/nrneph.2015.3","article-title":"Mechanisms of maladaptive repair after AKI leading to accelerated kidney ageing and CKD","volume":"11","author":"Ferenbach","year":"2015","journal-title":"Nat. Rev. Nephrol."},{"key":"B7","doi-asserted-by":"publisher","first-page":"279","DOI":"10.1016\/j.ydbio.2006.06.007","article-title":"The role of megalin (LRP-2\/Gp330) during development","volume":"296","author":"Fisher","year":"2006","journal-title":"Dev. Biology"},{"key":"B8","doi-asserted-by":"publisher","first-page":"2172","DOI":"10.3390\/cells9102172","article-title":"AQP2: mutations associated with congenital nephrogenic diabetes insipidus and regulation by post-translational modifications and protein-protein interactions","volume":"9","author":"Gao","year":"2020","journal-title":"Cells"},{"key":"B9","doi-asserted-by":"publisher","first-page":"3035","DOI":"10.1681\/ASN.2021030399","article-title":"Generation of distal renal segments involves a unique population of Aqp2 + progenitor cells","volume":"32","author":"Gao","year":"2021","journal-title":"J. Am. Soc. Nephrol. JASN"},{"key":"B10","doi-asserted-by":"publisher","first-page":"1357","DOI":"10.1681\/ASN.2021081105","article-title":"Aqp2(+) progenitor cells maintain and repair distal renal segments","volume":"33","author":"Gao","year":"2022","journal-title":"J. Am. Soc. Nephrol. JASN"},{"key":"B11","doi-asserted-by":"publisher","first-page":"1488","DOI":"10.1038\/nm.4437","article-title":"Enhancing the precision of genetic lineage tracing using dual recombinases","volume":"23","author":"He","year":"2017","journal-title":"Nat. Med."},{"key":"B12","doi-asserted-by":"publisher","first-page":"e2005233","DOI":"10.1371\/journal.pbio.2005233","article-title":"Jagged1\/Notch2 controls kidney fibrosis via Tfam-mediated metabolic reprogramming","volume":"16","author":"Huang","year":"2018","journal-title":"PLoS Biology"},{"key":"B13","doi-asserted-by":"publisher","first-page":"9226","DOI":"10.1073\/pnas.1100629108","article-title":"Repair of injured proximal tubule does not involve specialized progenitors","volume":"108","author":"Humphreys","year":"2011","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"B14","doi-asserted-by":"publisher","first-page":"e1","DOI":"10.1159\/000070812","article-title":"Following the expression of a kidney-specific gene from early development to adulthood","volume":"94","author":"Igarashi","year":"2004","journal-title":"Nephron Exp. Nephrol."},{"key":"B15","doi-asserted-by":"publisher","first-page":"F599","DOI":"10.1152\/ajprenal.1999.277.4.F599","article-title":"Ksp-cadherin gene promoter. II. Kidney-specific activity in transgenic mice","volume":"277","author":"Igarashi","year":"1999","journal-title":"Am. Journal Physiology"},{"key":"B16","doi-asserted-by":"publisher","first-page":"38963","DOI":"10.31083\/FBS38963","article-title":"Molecular basis of oxidative stress-induced acute kidney injury, kidney fibrosis, chronic kidney disease, and clinical significance of targeting reactive oxygen species-regulated pathways to treat kidney disease","volume":"17","author":"Kandel","year":"2025","journal-title":"Front. Biosci."},{"key":"B17","doi-asserted-by":"publisher","first-page":"861","DOI":"10.1016\/j.celrep.2015.12.071","article-title":"Sox9-Positive progenitor cells play a key role in renal tubule epithelial regeneration in mice","volume":"14","author":"Kang","year":"2016","journal-title":"Cell Rep."},{"key":"B18","doi-asserted-by":"publisher","first-page":"52","DOI":"10.1038\/s41572-021-00284-z","article-title":"Acute kidney injury","volume":"7","author":"Kellum","year":"2021","journal-title":"Nat. Rev. Dis. Prim."},{"key":"B19","doi-asserted-by":"publisher","first-page":"169","DOI":"10.1016\/j.stem.2008.05.020","article-title":"Six2 defines and regulates a multipotent self-renewing nephron progenitor population throughout Mammalian kidney development","volume":"3","author":"Kobayashi","year":"2008","journal-title":"Cell Stem Cell"},{"key":"B20","doi-asserted-by":"publisher","first-page":"673","DOI":"10.1007\/s00467-013-2669-3","article-title":"Controversies on the origin of proliferating epithelial cells after kidney injury","volume":"29","author":"Kusaba","year":"2014","journal-title":"Pediatr. Nephrol."},{"key":"B21","doi-asserted-by":"publisher","first-page":"1527","DOI":"10.1073\/pnas.1310653110","article-title":"Differentiated kidney epithelial cells repair injured proximal tubule","volume":"111","author":"Kusaba","year":"2014","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"B22","doi-asserted-by":"publisher","first-page":"1344","DOI":"10.1038\/s41467-018-03753-4","article-title":"Endocycle-related tubular cell hypertrophy and progenitor proliferation recover renal function after acute kidney injury","volume":"9","author":"Lazzeri","year":"2018","journal-title":"Nat. Commun."},{"key":"B23","doi-asserted-by":"publisher","first-page":"105294","DOI":"10.1016\/j.ebiom.2024.105294","article-title":"Renal tubular epithelial cells response to injury in acute kidney injury","volume":"107","author":"Li","year":"2024","journal-title":"EBioMedicine"},{"key":"B24","doi-asserted-by":"publisher","first-page":"1002","DOI":"10.1038\/s41401-024-01445-y","article-title":"Inhibition of HIF-Prolyl hydroxylase promotes renal tubule regeneration via the reprogramming of renal proximal tubular cells","volume":"46","author":"Li","year":"2025","journal-title":"Acta Pharmacol. Sin."},{"key":"B25","doi-asserted-by":"publisher","first-page":"143","DOI":"10.1038\/s43587-025-01024-8","article-title":"Cancer-cell-secreted DDAH1 induces TGF-\u03b21\/Smad3 signaling pathway to promote fibrosis and aging in lung","volume":"6","author":"Liu","year":"2026","journal-title":"Nat. Aging"},{"key":"B26","doi-asserted-by":"publisher","first-page":"1712","DOI":"10.1038\/ki.1996.253","article-title":"Renal aquaporins","volume":"49","author":"Mark","year":"1996","journal-title":"Kidney International"},{"key":"B27","doi-asserted-by":"publisher","first-page":"e64843","DOI":"10.1371\/journal.pone.0064843","article-title":"Kidney specific protein-positive cells derived from embryonic stem cells reproduce tubular structures in vitro and differentiate into renal tubular cells","volume":"8","author":"Morizane","year":"2014","journal-title":"PloS One"},{"key":"B28","doi-asserted-by":"publisher","first-page":"692","DOI":"10.3390\/biom9110692","article-title":"Notch signaling in kidney development, maintenance, and disease","volume":"9","author":"Mukherjee","year":"","journal-title":"Biomolecules"},{"key":"B29","doi-asserted-by":"publisher","first-page":"110","DOI":"10.1681\/ASN.2018040440","article-title":"Endogenous notch signaling in adult kidneys maintains segment-specific epithelial cell types of the distal tubules and collecting ducts to ensure water homeostasis","volume":"30","author":"Mukherjee","year":"","journal-title":"J. Am. Soc. Nephrol."},{"key":"B30","doi-asserted-by":"publisher","first-page":"1057","DOI":"10.1038\/ki.1995.389","article-title":"The aquaporin family of water channels in kidney","volume":"48","author":"Nielsen","year":"1995","journal-title":"Kidney International"},{"key":"B31","doi-asserted-by":"publisher","first-page":"58","DOI":"10.1016\/j.kint.2015.11.007","article-title":"Megalin and cubilin in proximal tubule protein reabsorption: from experimental models to human disease","volume":"89","author":"Nielsen","year":"2016","journal-title":"Kidney International"},{"key":"B32","doi-asserted-by":"publisher","first-page":"757","DOI":"10.1016\/j.stemcr.2016.03.008","article-title":"A subpopulation of label-retaining cells of the kidney papilla regenerates injured kidney medullary tubules","volume":"6","author":"Oliver","year":"2016","journal-title":"Stem Cell Rep."},{"key":"B33","doi-asserted-by":"publisher","first-page":"758","DOI":"10.1126\/science.aar2131","article-title":"Single-cell transcriptomics of the mouse kidney reveals potential cellular targets of kidney disease","volume":"360","author":"Park","year":"2018","journal-title":"Science"},{"key":"B34","doi-asserted-by":"publisher","first-page":"1270","DOI":"10.1016\/j.celrep.2014.04.018","article-title":"In vivo clonal analysis reveals lineage-restricted progenitor characteristics in Mammalian kidney development, maintenance, and regeneration","volume":"7","author":"Rinkevich","year":"2014","journal-title":"Cell Rep."},{"key":"B35","doi-asserted-by":"publisher","first-page":"4185","DOI":"10.1073\/pnas.022039799","article-title":"Directed evolution of the site specificity of cre recombinase","volume":"99","author":"Santoro","year":"2002","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"B36","doi-asserted-by":"publisher","first-page":"5214","DOI":"10.1038\/sj.emboj.7601381","article-title":"Six2 is required for suppression of nephrogenesis and progenitor renewal in the developing kidney","volume":"25","author":"Self","year":"2006","journal-title":"EMBO Journal"},{"key":"B37","doi-asserted-by":"publisher","first-page":"1824","DOI":"10.1097\/01.asn.0000016443.50138.cd","article-title":"A minimal Ksp-cadherin promoter linked to a green fluorescent protein reporter gene exhibits tissue-specific expression in the developing kidney and genitourinary tract","volume":"13","author":"Shao","year":"","journal-title":"J. Am. Soc. Nephrol. JASN"},{"key":"B38","doi-asserted-by":"publisher","first-page":"1837","DOI":"10.1097\/01.asn.0000016444.90348.50","article-title":"Epithelial-specific cre\/Lox recombination in the developing kidney and genitourinary tract","volume":"13","author":"Shao","year":"","journal-title":"J. Am. Soc. Nephrol. JASN"},{"key":"B39","doi-asserted-by":"publisher","first-page":"2010","DOI":"10.1038\/sj.bjc.6602597","article-title":"Expression of Ksp-cadherin during kidney development and in renal cell carcinoma","volume":"92","author":"Thedieck","year":"2005","journal-title":"Br. J. Cancer"},{"key":"B40","doi-asserted-by":"publisher","first-page":"1106","DOI":"10.1152\/ajprenal.00556.2020","article-title":"Deletion of Cdh16 Ksp-cadherin leads to a developmental delay in the ability to maximally concentrate urine in mouse","volume":"320","author":"Thomson","year":"2021","journal-title":"Am. Journal Physiology Ren. Physiology"},{"key":"B41","doi-asserted-by":"publisher","first-page":"228","DOI":"10.1007\/BF00860757","article-title":"Discovery of aquaporins: a breakthrough in research on renal water transport","volume":"9","author":"van Lieburg","year":"1995","journal-title":"Pediatr. Nephrology Berl. Ger."},{"key":"B42","doi-asserted-by":"publisher","first-page":"e0144734","DOI":"10.1371\/journal.pone.0144734","article-title":"Kinetics of label retaining cells in the developing rat kidneys","volume":"10","author":"Wang","year":"2015","journal-title":"PloS One"},{"key":"B43","doi-asserted-by":"publisher","first-page":"185","DOI":"10.1016\/s0925-4773(99)00074-x","article-title":"Kidney-specific cadherin (cdh16) is expressed in embryonic kidney, lung, and sex ducts","volume":"84","author":"Wertz","year":"1999","journal-title":"Mech. Development"},{"key":"B44","doi-asserted-by":"publisher","first-page":"869","DOI":"10.1016\/j.stem.2018.10.010","article-title":"Comparative analysis and refinement of human PSC-derived kidney organoid differentiation with single-cell transcriptomics","volume":"23","author":"Wu","year":"2018","journal-title":"Cell Stem Cell"},{"key":"B45","doi-asserted-by":"publisher","first-page":"174","DOI":"10.1038\/s41578-018-0027-6","article-title":"Tissue repair and regeneration with endogenous stem cells","volume":"3","author":"Xia","year":"2018","journal-title":"Nat. Rev. Mater."},{"key":"B46","doi-asserted-by":"publisher","first-page":"337","DOI":"10.1681\/ASN.2019070739","article-title":"Loss of histone H3 K79 methyltransferase Dot1l facilitates kidney fibrosis by upregulating endothelin 1 through histone deacetylase 2","volume":"31","author":"Zhang","year":"2020","journal-title":"J. Am. Soc. Nephrol."},{"key":"B47","doi-asserted-by":"publisher","first-page":"e2309752","DOI":"10.1002\/advs.202309752","article-title":"Identification of a novel ECM remodeling macrophage subset in AKI to CKD transition by integrative spatial and single-cell analysis","volume":"11","author":"Zhang","year":"2024","journal-title":"Adv. Science Weinheim, Baden-Wurttemberg, Ger."}],"container-title":["Frontiers in Cell and Developmental Biology"],"original-title":[],"link":[{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fcell.2026.1893353\/full","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T05:39:14Z","timestamp":1788932354000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fcell.2026.1893353\/full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,9]]},"references-count":47,"alternative-id":["10.3389\/fcell.2026.1893353"],"URL":"https:\/\/doi.org\/10.3389\/fcell.2026.1893353","relation":{},"ISSN":["2296-634X"],"issn-type":[{"value":"2296-634X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,9]]},"article-number":"1893353"}}