{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T06:25:26Z","timestamp":1787034326656,"version":"build-2736575974"},"reference-count":34,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T00:00:00Z","timestamp":1787011200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Cell Dev. Biol."],"abstract":"<jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>\n                      <jats:italic>Turmeric<\/jats:italic>\n                      and its major bioactive component, curcumin, have shown promising potential in the prevention and management of Alzheimer\u2019s disease (AD). However, the underlying molecular mechanisms and the contribution of turmeric residues remain incompletely understood. This study aimed to investigate the protective effects of curcumin and turmeric residue in an A\u03b21\u201042\u2010induced AD mouse model and to elucidate their metabolic regulatory mechanisms.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>The phytochemical profiles of turmeric and turmeric residue were characterized using UPLC\u2013MS\/MS combined with bioinformatics analysis. An A\u03b21\u201042\u2010induced AD mouse model was established, and mass spectrometry imaging (AFAI\u2010MSI) was applied to determine the optimal modeling time point. The therapeutic effects of curcumin and turmeric residue were evaluated through behavioral assessments, including the Morris water maze test, as well as histopathological and molecular analyses involving H&amp;amp;E staining, Nissl staining, immunofluorescence, and immunohistochemistry. Furthermore, non\u2010targeted metabolomics was performed to identify metabolic alterations associated with treatment.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>UPLC \u2010MS\/MS analysis identified 132 and 104 compounds in turmeric and turmeric residue, respectively, with 10 compounds showing significant differences in abundance between the two materials. AFAI\u2010MSI analysis indicated that three weeks after A\u03b21\u201042 injection represented an optimal time point for AD model establishment. Behavioral experiments demonstrated that both curcumin and turmeric residue significantly improved spatial learning and memory impairment in AD mice. Histological and molecular analyses revealed reduced neuronal damage and modulation of A\u03b21\u201042 and Iba\u20101 expression in brain tissues. Metabolomic profiling identified 25 differential plasma metabolites and 38 differential brain metabolites following treatment. Pathway enrichment analysis suggested that these metabolic alterations were mainly associated with ether lipid, sphingolipid, glycerophospholipid, glutamate, purine, and arachidonic acid metabolism.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Discussion<\/jats:title>\n                    <jats:p>These findings demonstrate that curcumin and turmeric residue exert neuroprotective effects against AD\u2010related pathological alterations by regulating metabolic disturbances and restoring metabolite homeostasis. The integration of chemical profiling, AFAI\u2010MSI, and metabolomics provides new insights into the therapeutic potential and metabolic mechanisms of turmeric\u2010derived resources in Alzheimer\u2019s disease.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.3389\/fcell.2026.1895521","type":"journal-article","created":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T05:38:09Z","timestamp":1787031489000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Integrated metabolomics and mass spectrometry imaging reveal the Anti-Alzheimer\u2019s mechanisms of turmeric and curcumin"],"prefix":"10.3389","volume":"14","author":[{"given":"Zehua","family":"Yang","sequence":"first","affiliation":[{"name":"School of Chinese Materia Medica Guangdong Pharmaceutical University Guangzhou China","place":["Guangzhou, Guangdong, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanyan","family":"Zeng","sequence":"additional","affiliation":[{"name":"School of Chinese Materia Medica Guangdong Pharmaceutical University Guangzhou China","place":["Guangzhou, Guangdong, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiahui","family":"Deng","sequence":"additional","affiliation":[{"name":"School of Chinese Materia Medica Guangdong Pharmaceutical University Guangzhou China","place":["Guangzhou, Guangdong, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuiping","family":"Zhang","sequence":"additional","affiliation":[{"name":"School of Chinese Materia Medica Guangdong Pharmaceutical University Guangzhou China","place":["Guangzhou, Guangdong, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Xue","sequence":"additional","affiliation":[{"name":"School of Chinese Materia Medica Guangdong Pharmaceutical University Guangzhou China","place":["Guangzhou, Guangdong, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiaohui","family":"Li","sequence":"additional","affiliation":[{"name":"School of Chinese Materia Medica Guangdong Pharmaceutical University Guangzhou China","place":["Guangzhou, Guangdong, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi","family":"Feng","sequence":"additional","affiliation":[{"name":"Hospital Pharmaceutical Preparation R&D Lab, Guangdong Provincial Hospital of Chinese Medicine","place":["Guangzhou, Guangdong, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu","family":"Zeng","sequence":"additional","affiliation":[{"name":"School of Chinese Materia Medica Guangdong Pharmaceutical University Guangzhou China","place":["Guangzhou, Guangdong, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,8,18]]},"reference":[{"key":"B1","first-page":"827","article-title":"U394 NI, institut francoi\u00e7 magendie, rue camille saint-saens\u00eb bordeaux cedex 33077, France\/raw. 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