{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T06:01:10Z","timestamp":1790834470700,"version":"4.1.0"},"reference-count":33,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Cell Dev. Biol."],"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>This study aimed to explore DDX17 protein expression as a diagnostic biomarker of hepatocellular carcinoma (HCC) and its roles in the occurrence and development of HCC.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>The proteomics sequencing method was applied to study nine samples of exosomes separated from HCC tissues and corresponding normal non-cancerous tissues. We also performed nanoflow cytometry analysis of DDX17 expression in serum-derived exosomes from 102 HCC patients, 58 persons with liver cirrhosis (LC), and 80 healthy donors (HDs). The feasibility of DDX17 as a diagnostic and prognostic biomarker was evaluated using receiver operating characteristic (ROC) curves. The effects of HCC-derived exosomal DDX17 on LX-2 cell proliferation, migration, and epithelial\u2013mesenchymal transition (EMT) were evaluated through cell proliferation, colony formation, wound healing, transwell migration, and western blotting assays.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>We found that exosomes produced by HCC cells had much higher levels of DDX17 than those produced by the adjacent non-cancerous tissues. ROC analysis showed that exosomal DDX17 has a strong ability to distinguish HCC with both high sensitivity and high specificity. If the exosomal DDX17 result is negative, then we expect it to be a new kind of diagnostic biomarker. Moreover, we observed that DDX17-positive exosome subtypes isolated from the HepG2 cell line increased cell proliferation, migration, invasion, and EMT in the LX-2 cell line.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>DDX17-positive exosomes are potential diagnostic biomarkers of HCC and are expected to promote tumor growth, migration, invasion, and EMT.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.3389\/fcell.2026.1897138","type":"journal-article","created":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T05:42:03Z","timestamp":1790833323000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Serum exosomal DDX17 in the diagnosis and action mechanism of hepatocellular carcinoma"],"prefix":"10.3389","volume":"14","author":[{"given":"Defa","family":"Huang","sequence":"first","affiliation":[{"name":"Laboratory Medicine, First Affiliated Hospital of Gannan Medical University","place":["Ganzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Minghong","family":"Zhao","sequence":"additional","affiliation":[{"name":"Laboratory Medicine, Guizhou Aerospace Hospital","place":["Zunyi, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meijin","family":"Liu","sequence":"additional","affiliation":[{"name":"Laboratory Medicine, People\u2019s Hospital of Ganzhou Economic Development Zone","place":["Ganzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tao","family":"Chen","sequence":"additional","affiliation":[{"name":"The First School of Clinical Medicine, Gannan Medical University","place":["Ganzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zihao","family":"Chen","sequence":"additional","affiliation":[{"name":"The First School of Clinical Medicine, Gannan Medical University","place":["Ganzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuhuan","family":"Xie","sequence":"additional","affiliation":[{"name":"The First School of Clinical Medicine, Gannan Medical University","place":["Ganzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haiyang","family":"Yu","sequence":"additional","affiliation":[{"name":"The First School of Clinical Medicine, Gannan Medical University","place":["Ganzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuqian","family":"Zhong","sequence":"additional","affiliation":[{"name":"The First School of Clinical Medicine, Gannan Medical University","place":["Ganzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junyun","family":"Huang","sequence":"additional","affiliation":[{"name":"Laboratory Medicine, First Affiliated Hospital of Gannan Medical University","place":["Ganzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dingyu","family":"Rao","sequence":"additional","affiliation":[{"name":"Department of Cardiothoracic Surgery, The First Affiliated Hospital of Gannan Medical University","place":["Ganzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,10,1]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"42","DOI":"10.1016\/j.cellsig.2015.11.004","article-title":"DDX17 (P72), a Sox2 binding partner, promotes stem-like features conferred by Sox2 in a small cell population in estrogen receptor-positive breast cancer","volume":"28","author":"Alqahtani","year":"2016","journal-title":"Cell. 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