{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T07:16:13Z","timestamp":1788419773505,"version":"build-2803163510"},"reference-count":55,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T00:00:00Z","timestamp":1788393600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100016807","name":"Natural Science Foundation of Shenyang Municipality","doi-asserted-by":"publisher","award":["2025-MSLH-254"],"award-info":[{"award-number":["2025-MSLH-254"]}],"id":[{"id":"10.13039\/100016807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100016807","name":"Natural Science Foundation of Shenyang Municipality","doi-asserted-by":"publisher","award":["2025JH5\/10400041"],"award-info":[{"award-number":["2025JH5\/10400041"]}],"id":[{"id":"10.13039\/100016807","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Cell Dev. Biol."],"abstract":"<jats:p>The objective of this study was to evaluate the effects of low\u2010protein dietary supplementation with NCG on productivity, egg quality and possible regulatory mechanisms in laying Hens. A total of 150 Beijing Type 2 laying hens at 300 days of age were randomly assigned to five groups: a control group, a low-protein diet group (LP), and three low\u2010protein diet groups supplemented with 0.06%, 0.12%, or 0.18% NCG. Daily feed intake, Egg number and egg weight were recorded daily on a replicate basis to calculate the egg production rate, average feed intake (ADFI), feed conversion ratio (FCR) and evaluate egg quality. Blood samples were collected from the jugular vein, and the serum was obtained to determine various biochemical parameters using an automatic biochemical analyzer. The protein secretory region of the magnum part of the oviduct was collected and immediately frozen in liquid nitrogen for RNA sequencing analysis. The RNA\u2010seq results were subsequently validated by real-time quantitative PCR (RT\u2010qPCR) using Pearson\u2019s correlation analysis. Supplementation with 0.06% and 0.18% NCG in low\u2010protein diets significantly increased egg weight compared with the LP group (P &amp;lt; 0.05). Albumen height and Haugh unit were significantly increased in the 0.06% NCG group compared with the other groups (P &amp;lt; 0.05). The 0.12% NCG supplementation significantly increased serum BUN content (P &amp;lt; 0.05), whereas the 0.18% NCG supplementation significantly decreased serum phosphorus content (P &amp;lt; 0.05). Serum ALB and NO concentrations were significantly increased in the 0.06% and 0.18% NCG groups compared with the LP group (P &amp;lt; 0.05). Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses were conducted to identify differentially expressed genes and enriched signaling pathways. The results demonstrated that the genes suppressor of cytokine signaling 3 (SOCS3), integrin subunit alpha 8 (ITGA8), platelet\u2010derived growth factor receptor alpha (PDGFRA), angiotensin\u2010converting enzyme (ACE), A disintegrin and metalloproteinase with thrombospondin motifs 1 (ADAMTS\u20101), and apolipoprotein L domain containing 1 (APOLD1), which are associated with the PI3K\/AKT\/mTOR signaling pathway, were regulated by NCG supplementation under low\u2010protein dietary conditions. In conclusion, supplementation with 0.06% NCG in a low-protein diet improved egg weight and egg quality by regulating protein synthesis and secretion in the magnum part of the hen\u2019s oviduct.<\/jats:p>","DOI":"10.3389\/fcell.2026.1897620","type":"journal-article","created":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T06:45:00Z","timestamp":1788417900000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["N-carbamylglutamate (NCG) supplementation in low-protein diets improving egg quality by regulating protein metabolism pathway of oviduct in laying hens"],"prefix":"10.3389","volume":"14","author":[{"given":"Mi","family":"Wang","sequence":"first","affiliation":[{"name":"College of Animal Husbandry and Veterinary Medicine, Jinzhou Medical University","place":["Jinzhou, China"]},{"name":"Collaborative Innovation Center for Prevention and Control of Zoonoses, Jinzhou Medical University","place":["Jinzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiulin","family":"Liu","sequence":"additional","affiliation":[{"name":"College of Animal Husbandry and Veterinary Medicine, Jinzhou Medical University","place":["Jinzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Di","family":"Han","sequence":"additional","affiliation":[{"name":"Liaoning Provincial Department of Agriculture and Rural Affairs, Liaoning Provincial Agricultural and Rural Development Service Center","place":["Shenyang, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiabo","family":"Li","sequence":"additional","affiliation":[{"name":"College of Animal Husbandry and Veterinary Medicine, Jinzhou Medical University","place":["Jinzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xing","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Animal Husbandry and Veterinary Medicine, Jinzhou Medical University","place":["Jinzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Ma","sequence":"additional","affiliation":[{"name":"College of Animal Husbandry and Veterinary Medicine, Jinzhou Medical University","place":["Jinzhou, China"]},{"name":"Collaborative Innovation Center for Prevention and Control of Zoonoses, Jinzhou Medical University","place":["Jinzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chunqiang","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Animal Husbandry and Veterinary Medicine, Jinzhou Medical University","place":["Jinzhou, China"]},{"name":"Collaborative Innovation Center for Prevention and Control of Zoonoses, Jinzhou Medical University","place":["Jinzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,9,3]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"1028","DOI":"10.9755\/ejfa.2022.v34.i12.2972","article-title":"Effect of N-carbamylglutamate on amino acids and blood biochemistry in young Male goats","volume":"34","author":"Al-Ani","year":"2022","journal-title":"Emir. J. Food Agric."},{"key":"B2","doi-asserted-by":"publisher","first-page":"e20180230","DOI":"10.1590\/0001-3765202020180230","article-title":"Influence of low-protein diet with different levels of amino acids on laying hen performance, quality and egg composition","volume":"92","author":"Alagawany","year":"2020","journal-title":"An. Acad. Bras. Ci\u00eancias"},{"key":"B3","doi-asserted-by":"publisher","first-page":"1015","DOI":"10.2147\/NDT.S75717","article-title":"Activation of mTOR: a culprit of Alzheimer\u2019s disease?","volume":"11","author":"Cai","year":"2015","journal-title":"Neuropsychiatric Disease Treatment"},{"key":"B4","doi-asserted-by":"publisher","first-page":"1084","DOI":"10.1186\/1471-2164-15-1084","article-title":"RNA-Seq analysis of broiler liver transcriptome reveals novel responses to high ambient temperature","volume":"15","author":"Coble","year":"2014","journal-title":"BMC Genomics"},{"key":"B5","doi-asserted-by":"publisher","first-page":"226","DOI":"10.1186\/1471-2164-15-226","article-title":"Transcriptional profiling of mammary gland in holstein cows with extremely different milk protein and fat percentage using RNA sequencing","volume":"15","author":"Cui","year":"2014","journal-title":"BMC Genomics"},{"key":"B6","doi-asserted-by":"publisher","first-page":"3963","DOI":"10.3382\/ps\/pez132","article-title":"Effect of a low protein diet on chicken ceca microbiome and productive performances","volume":"98","author":"De Cesare","year":"2019","journal-title":"Poult. Science"},{"key":"B7","doi-asserted-by":"publisher","first-page":"e13191","DOI":"10.1111\/eci.13191","article-title":"Apold1 deficiency associates with increased arterial thrombosis in vivo","volume":"50","author":"Diaz-Ca\u00f1estro","year":"2020","journal-title":"Eur. Journal Clinical Investigation"},{"key":"B8","doi-asserted-by":"publisher","first-page":"268","DOI":"10.2450\/2008.0080-08","article-title":"Clinical use of albumin in hepatology","volume":"7","author":"Garcovich","year":"2009","journal-title":"Blood Transfus."},{"key":"B9","doi-asserted-by":"publisher","first-page":"31972","DOI":"10.1074\/jbc.M303170200","article-title":"Tyrosine phosphorylation disrupts elongin interaction and accelerates SOCS3 degradation","volume":"278","author":"Haan","year":"2003","journal-title":"J. Biol. Chem."},{"key":"B10","doi-asserted-by":"publisher","first-page":"7415","DOI":"10.1523\/jneurosci.19-17-07415.1999","article-title":"Regulated expression and subcellular localization of syndecan heparan sulfate Proteoglycans and the syndecan-binding protein CASK\/LIN-2 during rat brain development","volume":"19","author":"Hsueh","year":"1999","journal-title":"J. Neurosci."},{"key":"B11","doi-asserted-by":"publisher","first-page":"1483","DOI":"10.7150\/ijbs.27173","article-title":"The PI3K\/AKT pathway in obesity and type 2 diabetes","volume":"14","author":"Huang","year":"2018","journal-title":"Int. Journal Biological Sciences"},{"key":"B12","doi-asserted-by":"publisher","first-page":"8","DOI":"10.1016\/j.cbi.2018.12.001","article-title":"Carbon disulfide induces embryo loss by perturbing the expression of the mTOR signalling pathway in uterine tissue in mice","volume":"300","author":"Huang","year":"2019","journal-title":"Chemico-Biological Interact."},{"key":"B13","doi-asserted-by":"publisher","first-page":"12934","DOI":"10.3390\/app132312934","article-title":"The order of limiting amino acids in a Wheat\u2013sorghum-based reduced-protein diet for laying hens","volume":"13","author":"Jahan","year":"2023","journal-title":"Appl. Sci."},{"key":"B14","doi-asserted-by":"publisher","first-page":"1208","DOI":"10.1016\/j.jhep.2013.07.015","article-title":"Adiponectin and resistin protect steatotic livers undergoing transplantation","volume":"59","author":"Jim\u00e9nez-Castro","year":"2013","journal-title":"J. Hepatology"},{"key":"B15","doi-asserted-by":"publisher","first-page":"905","DOI":"10.3382\/ps.0710905","article-title":"Performance of growing pullets and laying hens fed low-protein, amino acid-supplemented diets","volume":"71","author":"Keshavarz","year":"1992","journal-title":"Poult. Sci."},{"key":"B16","doi-asserted-by":"publisher","first-page":"303","DOI":"10.1007\/s13273-022-00259-5","article-title":"Effects of Agarum clathratum extract on cell death and calcium ion levels of ovarian cancer cell","volume":"19","author":"Kim","year":"2023","journal-title":"Mol. and Cell. Toxicol."},{"key":"B17","doi-asserted-by":"publisher","first-page":"489","DOI":"10.1111\/asj.12021","article-title":"Effects of low protein diet and low protein diet supplemented with synthetic essential amino acids on meat quality of broiler chickens","volume":"84","author":"Kobayashi","year":"2013","journal-title":"Animal Sci. J."},{"key":"B18","doi-asserted-by":"publisher","first-page":"691","DOI":"10.4314\/tjpr.v19i4.3","article-title":"MiR-624-5p enhances cell resistance against cisplatin via PDGFRA\/Stat3\/PI3K axis in ovarian cancer","volume":"19","author":"Lin","year":"2020","journal-title":"Trop. J. Pharm. Res."},{"key":"B19","doi-asserted-by":"publisher","first-page":"1137","DOI":"10.1007\/s12033-024-01114-9","article-title":"Revealing a novel methylated integrin alpha-8 related to extracellular matrix and anoikis resistance using proteomic analysis in the immune microenvironment of lung adenocarcinoma","volume":"67","author":"Liu","year":"2025","journal-title":"Mol. Biotechnol."},{"key":"B20","doi-asserted-by":"publisher","first-page":"4085","DOI":"10.1016\/j.psj.2020.04.004","article-title":"Improvement of eggshell quality by dietary N-carbamylglutamate supplementation in laying chickens","volume":"99","author":"Ma","year":"2020","journal-title":"Poult. Science"},{"key":"B21","doi-asserted-by":"publisher","first-page":"1110801","DOI":"10.3389\/fvets.2023.1110801","article-title":"Production performance, egg quality, and uterine gene expression for layers as affected by N-Carbamylglutamate supplementation","volume":"10","author":"Ma","year":"","journal-title":"Front. Veterinary Sci."},{"key":"B22","doi-asserted-by":"publisher","first-page":"621","DOI":"10.1111\/jpn.13714","article-title":"Effects of dietary supplementation of N-carbamylglutamate on the haematology parameters, secondary sexual characteristics and testicular gene expression in roosters","volume":"107","author":"Ma","year":"","journal-title":"J. Animal Physiology Animal Nutr."},{"key":"B23","doi-asserted-by":"publisher","first-page":"949","DOI":"10.1016\/j.psj.2019.10.035","article-title":"Broilers fed a low protein diet supplemented with synthetic amino acids maintained growth performance and retained intestinal integrity while reducing nitrogen excretion when raised under poor sanitary conditions","volume":"99","author":"Macelline","year":"2020","journal-title":"Poult. Science"},{"key":"B24","doi-asserted-by":"publisher","first-page":"493","DOI":"10.1111\/ahe.12370","article-title":"Ultrastructure of the tubular glands in the isthmus region of the oviduct in laying and natural moulting commercial egg-type chickens","volume":"47","author":"Madekurozwa","year":"2018","journal-title":"Anat. Histologia, Embryologia"},{"key":"B25","doi-asserted-by":"publisher","first-page":"28","DOI":"10.51470\/PLANTARCHIVES.2021.v21.S1.007","article-title":"Effect of additives N-carbamylglutamate with urea on feed intake and daily gain of awassi lambs","volume":"21","author":"Mahdi","year":"2021","journal-title":"Plant Arch."},{"key":"B26","doi-asserted-by":"publisher","first-page":"e19922","DOI":"10.1590\/s2175-97902022e19922","article-title":"Zofenopril antitumor activity in mice bearing ehrlich solid carcinoma: modulation of PI3K\/AKT signaling pathway","volume":"58","author":"Mansour","year":"2022","journal-title":"Braz. J. Pharm. Sci."},{"key":"B27","doi-asserted-by":"publisher","first-page":"213","DOI":"10.1080\/00071660120048474","article-title":"Nitrogen retention and performance of brown laying hens on diets with different protein content and constant concentration of amino acids and energy","volume":"42","author":"Meluzzi","year":"2001","journal-title":"Br. Poult. Sci."},{"key":"B28","doi-asserted-by":"publisher","first-page":"1096","DOI":"10.1095\/biolreprod.103.023911","article-title":"Adamts-1 is essential for the development and function of the urogenital System1","volume":"70","author":"Mittaz","year":"2004","journal-title":"Biol. Reproduction"},{"key":"B29","doi-asserted-by":"publisher","first-page":"2076","DOI":"10.1248\/bpb.b19-00601","article-title":"The cyclitol L-(+)-bornesitol as an active marker for the cardiovascular activity of the Brazilian medicinal plant Hancornia speciosa","volume":"42","author":"Moreira","year":"2019","journal-title":"Biol. Pharm. Bull."},{"key":"B30","doi-asserted-by":"publisher","first-page":"3242","DOI":"10.3390\/cancers3033242","article-title":"Aberrant signaling pathways in glioma","volume":"3","author":"Nakada","year":"2011","journal-title":"Cancers"},{"key":"B31","doi-asserted-by":"publisher","first-page":"2252","DOI":"10.3390\/agriculture15212252","article-title":"Optimising energy-to-protein ratio in practical reduced-protein diets for laying hens","volume":"15","author":"Nawab","year":"2025","journal-title":"Agric. Basel"},{"key":"B32","doi-asserted-by":"publisher","first-page":"2631","DOI":"10.1007\/s11033-019-04661-6","article-title":"Pro-atherogenic proteoglycanase ADAMTS-1 is down-regulated by lauric acid through PI3K and JNK signaling pathways in THP-1 derived macrophages","volume":"46","author":"Ong","year":"2019","journal-title":"Mol. Biology Reports"},{"key":"B33","doi-asserted-by":"publisher","first-page":"766","DOI":"10.1080\/00071668.2014.970125","article-title":"Valine, isoleucine, arginine and glycine supplementation of low-protein diets for broiler chickens during the starter and grower phases","volume":"55","author":"Ospina-Rojas","year":"2014","journal-title":"Br. Poult. Sci."},{"key":"B34","doi-asserted-by":"publisher","first-page":"9751","DOI":"10.3390\/ijms14059751","article-title":"Resistin promotes the expression of vascular endothelial growth factor in ovary carcinoma cells","volume":"14","author":"Pang","year":"2013","journal-title":"Int. Journal Molecular Sciences"},{"key":"B35","doi-asserted-by":"publisher","first-page":"07012","DOI":"10.1621\/nrs.07012","article-title":"Control of oocyte release by progesterone receptor-regulated gene expression","volume":"7","author":"Robker","year":"2009","journal-title":"Nucl. Receptor Signaling"},{"key":"B36","doi-asserted-by":"publisher","first-page":"S152","DOI":"10.2337\/diabetes.53.2007.S152","article-title":"Leptin effects on pancreatic \u03b2-cell gene expression and function","volume":"53","author":"Seufert","year":"2004","journal-title":"Diabetes"},{"key":"B37","doi-asserted-by":"publisher","first-page":"1345","DOI":"10.1172\/jci8635","article-title":"ADAMTS-1: a metalloproteinase-disintegrin essential for normal growth, fertility, and organ morphology and function","volume":"105","author":"Shindo","year":"2000","journal-title":"J. Clinical Investigation"},{"key":"B38","doi-asserted-by":"publisher","first-page":"470","DOI":"10.1080\/00071668.2015.1054258","article-title":"L-arginine supplementation of reduced-protein diets improves pulmonary hypertensive response in broiler chickens reared at high altitude","volume":"56","author":"Sharifi","year":"2015","journal-title":"Br. Poult. Sci."},{"key":"B39","unstructured":"PI3k in human oesophageal squamous carcinoma cells: a critical modulator in the PKB signalling pathway\n          \n          \n            \n              Shaw\n              N.\n            \n          \n          \n          2011"},{"key":"B40","doi-asserted-by":"publisher","first-page":"e112896","DOI":"10.1371\/journal.pone.0112896","article-title":"Growth hormone-regulated mRNAs and miRNAs in chicken hepatocytes","volume":"9","author":"Wang","year":"2014","journal-title":"PloS One"},{"key":"B41","doi-asserted-by":"publisher","first-page":"63","DOI":"10.1186\/s40104-018-0277-6","article-title":"N-carbamylglutamate restores nitric oxide synthesis and attenuates high altitude-induced pulmonary hypertension in holstein heifers ascended to high altitude","volume":"9","author":"Wang","year":"2018","journal-title":"J. Animal Sci. Biotechnol."},{"key":"B42","doi-asserted-by":"publisher","first-page":"1364","DOI":"10.3382\/ps\/pew321","article-title":"Effects of low-protein diets on growth performance and carcass yields of growing French meat quails (france coturnix coturnix)","volume":"96","author":"Wen","year":"2017","journal-title":"Poult. Sci."},{"key":"B43","doi-asserted-by":"publisher","first-page":"2783S","DOI":"10.1093\/jn\/134.10.2783S","article-title":"Arginine nutrition in neonatal pigs","volume":"134","author":"Wu","year":"2004","journal-title":"J. Nutr."},{"key":"B44","doi-asserted-by":"publisher","first-page":"187","DOI":"10.1016\/j.anireprosci.2012.05.002","article-title":"Effect of dietary arginine and N-carbamoylglutamate supplementation on reproduction and gene expression of eNOS, VEGFA and PlGF1 in placenta in late pregnancy of sows","volume":"132","author":"Wu","year":"2012","journal-title":"Anim. Reprod. Sci."},{"key":"B45","doi-asserted-by":"publisher","first-page":"1102","DOI":"10.3390\/agriculture14071102","article-title":"Dietary crude protein reduction with addition of crystalline amino acids in growing pekin ducks housed in cascading cages: influence on growth performance, carcass traits, and apparent nutrient digestibility","volume":"14","author":"Wu","year":"2024","journal-title":"Agriculture"},{"key":"B46","doi-asserted-by":"publisher","first-page":"e13159","DOI":"10.1111\/aji.13159","article-title":"Transcriptome analysis reveals differentially expressed genes and pathways for oviduct development and defense in prelaying and laying hens","volume":"82","author":"Yin","year":"2019","journal-title":"Am. J. Reproductive Immunol."},{"key":"B47","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0041192","volume-title":"N-carbamylglutamate Enhances Pregnancy Outcome in Rats Through Activation of the PI3K\/PKB\/mTOR Signaling Pathway","author":"Zeng","year":"2012"},{"key":"B48","article-title":"Effect of different levels of protein and methionine on performance of baheij laying hens and environmental pollution","author":"Zeweil","year":"2011"},{"key":"B49","doi-asserted-by":"publisher","first-page":"923","DOI":"10.1093\/jn\/nxz016","article-title":"N-Carbamylglutamate and l-Arginine promote intestinal absorption of amino acids by regulating the mTOR signaling pathway and amino acid and peptide transporters in suckling lambs with intrauterine growth restriction","volume":"149","author":"Zhang","year":"2019","journal-title":"J. Nutr."},{"key":"B50","doi-asserted-by":"publisher","first-page":"623","DOI":"10.1530\/rep-16-0067","article-title":"N-carbamylglutamate and L-arginine improved maternal and placental development in underfed ewes","volume":"151","author":"Zhang","year":"2016","journal-title":"Reproduction"},{"key":"B51","doi-asserted-by":"publisher","first-page":"6374","DOI":"10.1039\/c9fo01752f","article-title":"N-carbamylglutamate and l-arginine promote intestinal function in suckling lambs with intrauterine growth restriction by regulating antioxidant capacity via a nitric oxide-dependent pathway","volume":"10","author":"Zhang","year":"2019","journal-title":"Food Funct."},{"key":"B52","doi-asserted-by":"publisher","first-page":"1095","DOI":"10.1016\/j.aninu.2021.06.004","article-title":"Dietary rumen-protected L-arginine or N-carbamylglutamate attenuated fetal hepatic inflammation in undernourished ewes suffering from intrauterine growth restriction","volume":"7","author":"Zhang","year":"2021","journal-title":"Anim. Nutr."},{"key":"B53","doi-asserted-by":"publisher","first-page":"102323","DOI":"10.1016\/j.psj.2022.102323","article-title":"Transcriptome and metabolome analyses reveal the regulatory effects of compound probiotics on cecal metabolism in heat-stressed broilers","volume":"102","author":"Zhang","year":"2023","journal-title":"Poult. Sci."},{"key":"B54","doi-asserted-by":"publisher","first-page":"825","DOI":"10.3390\/agriculture11090825","article-title":"N-Carbamylglutamate promotes follicular development by modulating cholesterol metabolism in yak ovaries","volume":"11","author":"Zhou","year":"2021","journal-title":"Agriculture"},{"key":"B55","doi-asserted-by":"publisher","first-page":"2212","DOI":"10.3945\/jn.115.216333","article-title":"Maternal N-Carbamylglutamate supplementation during early pregnancy enhances embryonic survival and development through modulation of the endometrial proteome in gilts","volume":"145","author":"Zhu","year":"2015","journal-title":"J. Nutr."}],"container-title":["Frontiers in Cell and Developmental Biology"],"original-title":[],"link":[{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fcell.2026.1897620\/full","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T06:45:02Z","timestamp":1788417902000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fcell.2026.1897620\/full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,3]]},"references-count":55,"alternative-id":["10.3389\/fcell.2026.1897620"],"URL":"https:\/\/doi.org\/10.3389\/fcell.2026.1897620","relation":{},"ISSN":["2296-634X"],"issn-type":[{"value":"2296-634X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,3]]},"article-number":"1897620"}}