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Cell Dev. Biol."],"abstract":"<jats:p>Primary cilia have recently been identified in mammalian spermatocytes, but their developmental regulation during testicular maturation remains poorly understood. Here, we investigate the dynamics of ciliogenesis during mouse puberty and show that primary cilia are not an intrinsic feature of spermatocytes during the first wave of meiosis, initiated at 8 days post partum (dpp). Ciliogenesis begins only at day 20 dpp, where cilia are detected across all stages of prophase I, indicating no direct association with synapsis or desynapsis. We further found that Aurora kinase A is associated with cilia disassembly in late diplotene and that spermatocytes retaining a polymerized cilium fail to assemble a bipolar spindle. In addition, proteomic analysis defined the 19\u201321 dpp period as a key developmental window associated with ciliogenesis and flagellogenesis. We also identified ciliated spermatocytes in the human testis, providing the first evidence of meiotic cilia in human meiocytes. This work reveals that ciliogenesis is a developmentally regulated process during testicular maturation in mouse.<\/jats:p>","DOI":"10.3389\/fcell.2026.1910205","type":"journal-article","created":{"date-parts":[[2026,8,14]],"date-time":"2026-08-14T05:32:44Z","timestamp":1786685564000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["The dynamics of ciliogenesis in prepubertal mouse meiosis reveals new clues about testis maturation"],"prefix":"10.3389","volume":"14","author":[{"given":"I.","family":"P\u00e9rez-Moreno","sequence":"first","affiliation":[{"name":"Unidad de Biolog\u00eda Celular, Departamento de Biolog\u00eda, Universidad Aut\u00f3noma de Madrid","place":["Madrid, Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P.","family":"L\u00f3pez-Jim\u00e9nez","sequence":"additional","affiliation":[{"name":"Unidad de Biolog\u00eda Celular, Departamento de Biolog\u00eda, Universidad Aut\u00f3noma de Madrid","place":["Madrid, Spain"]},{"name":"MRC Laboratory of Medical Sciences","place":["London, United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H.","family":"Zapata-Polo","sequence":"additional","affiliation":[{"name":"Unidad de Biolog\u00eda Celular, Departamento de Biolog\u00eda, Universidad Aut\u00f3noma de Madrid","place":["Madrid, Spain"]},{"name":"Centro de Investigaci\u00f3n Biom\u00e9dica en Red en Enfermedades Cardiovasculares (CIBERCV)","place":["Madrid, Spain"]},{"name":"Vascular Research Laboratory, IIS-Fundaci\u00f3n Jim\u00e9nez D\u00edaz","place":["Madrid, Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"P\u00e9rez-Mart\u00edn","sequence":"additional","affiliation":[{"name":"Unidad de Biolog\u00eda Celular, Departamento de Biolog\u00eda, Universidad Aut\u00f3noma de Madrid","place":["Madrid, Spain"]},{"name":"Centro de Investigaci\u00f3n Biom\u00e9dica en Red en Enfermedades Cardiovasculares (CIBERCV)","place":["Madrid, Spain"]},{"name":"Department of Pharmacology, School of Medicine, Instituto de Investigaci\u00f3n Sanitaria Gregorio Mara\u00f1\u00f3n, Universidad Complutense","place":["Madrid, Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"L\u00f3pez-Panad\u00e9s","sequence":"additional","affiliation":[{"name":"Genome Integrity and Instability Group, Institut de Biotecnologia i Biomedicina, Universitat Aut\u00f2noma de Barcelona","place":["Cerdanyola del Vall\u00e8s, Spain"]},{"name":"Cytology and Histology Unit, Department of Cell Biology, Physiology and Immunology, Universitat Aut\u00f2noma de Barcelona","place":["Cerdanyola del Vall\u00e8s, Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Urtasun-Elizari","sequence":"additional","affiliation":[{"name":"MRC Laboratory of Medical Sciences","place":["London, United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P.","family":"Barbeito","sequence":"additional","affiliation":[{"name":"Instituto de Investigaciones Biom\u00e9dicas Sols-Morreale (IIBM), UAM-CSIC","place":["Madrid, Spain"]},{"name":"Departamento de Bioqu\u00edmica, Facultad de Medicina, Universidad Aut\u00f3noma de Madrid","place":["Madrid, Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"I.","family":"Roig","sequence":"additional","affiliation":[{"name":"Genome Integrity and Instability Group, Institut de Biotecnologia i Biomedicina, Universitat Aut\u00f2noma de Barcelona","place":["Cerdanyola del Vall\u00e8s, Spain"]},{"name":"Cytology and Histology Unit, Department of Cell Biology, Physiology and Immunology, Universitat Aut\u00f2noma de Barcelona","place":["Cerdanyola del Vall\u00e8s, Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F. 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