{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T06:03:55Z","timestamp":1789625035288,"version":"build-2803163510"},"reference-count":50,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T00:00:00Z","timestamp":1789603200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["24K01937"],"award-info":[{"award-number":["24K01937"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Cell Dev. Biol."],"abstract":"<jats:p>\n                    The preimplantation period is a critical nutrient-sensing window influencing metabolic disease risk, as established by the developmental origins of health and disease theory (DOHaD). In this study, we examined the postnatal consequences of increased glutamine concentration, an important energy source, by altering embryonic metabolic development.\n                    <jats:italic>In vivo<\/jats:italic>\n                    -fertilised ICR mouse embryos were cultured in modified Chatot-Ziomek-Bavister medium with 1\u00a0mM\u00a0L-glutamine (Gln) and bovine serum albumin (BSA) as the control and compared with elevated Gln media (2\u00a0mM) with bovine serum albumin (+Gln) or without BSA (+Gln-BSA). Early embryonic developmental analysis showed that both +Gln and +Gln-BSA presented reduced blastocyst cell number, primarily in trophectoderm cells, associated with increased apoptosis. Embryos showed altered metabolic homeostasis, including reduced mitochondrial membrane potential and mitochondrial morphology, altered autophagy-related activity (indicated by discrepancies between the DAPGreen assay and LC3-positive autophagosomes), and reduced heterochromatin methylation signals (H3K27me3). Offspring derived from these embryos subsequently exhibited irregular body weight trajectories, reduced survival, and impaired glucose tolerance. These findings indicate that\n                    <jats:italic>in vitro<\/jats:italic>\n                    exposure to elevated glutamine during early development is associated with altered mitochondrial dynamics and autophagy-related responses, interrupting intracellular mechanisms with lifelong metabolic consequences.\n                  <\/jats:p>","DOI":"10.3389\/fcell.2026.1920196","type":"journal-article","created":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T05:33:52Z","timestamp":1789623232000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Excess glutamine in embryo culture media alters mitochondrial and autophagy-related dynamics associated with impaired postnatal development"],"prefix":"10.3389","volume":"14","author":[{"given":"Norermi Firzana","family":"Alfian","sequence":"first","affiliation":[{"name":"Department of Integrated Applied Life Science, Integrated Graduate School of Medicine, Engineering, and Agricultural Sciences, University of Yamanashi","place":["Kofu, Yamanashi, Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yui","family":"Maruhashi","sequence":"additional","affiliation":[{"name":"Department of Integrated Applied Life Science, Integrated Graduate School of Medicine, Engineering, and Agricultural Sciences, University of Yamanashi","place":["Kofu, Yamanashi, Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masahiro","family":"Horizoe","sequence":"additional","affiliation":[{"name":"Department of Integrated Applied Life Science, Integrated Graduate School of Medicine, Engineering, and Agricultural Sciences, University of Yamanashi","place":["Kofu, Yamanashi, Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wasim","family":"Bari","sequence":"additional","affiliation":[{"name":"Faculty of Life and Environmental Sciences, University of Yamanashi","place":["Kofu, Yamanashi, Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shiori","family":"Ishiyama","sequence":"additional","affiliation":[{"name":"Faculty of Life and Environmental Sciences, University of Yamanashi","place":["Kofu, Yamanashi, Japan"]},{"name":"Graduate School of Life and Environmental Sciences, Integrated Graduate School of Medicine, Engineering and Agricultural Science, University of Yamanashi","place":["Kofu, Yamanashi, Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kazuki","family":"Mochizuki","sequence":"additional","affiliation":[{"name":"Faculty of Life and Environmental Sciences, University of Yamanashi","place":["Kofu, Yamanashi, Japan"]},{"name":"Graduate School of Life and Environmental Sciences, Integrated Graduate School of Medicine, Engineering and Agricultural Science, University of Yamanashi","place":["Kofu, Yamanashi, Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Satoshi","family":"Kishigami","sequence":"additional","affiliation":[{"name":"Department of Integrated Applied Life Science, Integrated Graduate School of Medicine, Engineering, and Agricultural Sciences, University of Yamanashi","place":["Kofu, Yamanashi, Japan"]},{"name":"Faculty of Life and Environmental Sciences, University of Yamanashi","place":["Kofu, Yamanashi, Japan"]},{"name":"Graduate School of Life and Environmental Sciences, Integrated Graduate School of Medicine, Engineering and Agricultural Science, University of Yamanashi","place":["Kofu, Yamanashi, Japan"]},{"name":"Center for Advanced Assisted Reproductive Technologies, University of Yamanashi","place":["Kofu, Yamanashi, Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,9,17]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"117392","DOI":"10.1016\/j.theriogenology.2025.117392","article-title":"Inverse embryonic responses of in vivo and in vitro fertilized mouse embryos to vitamin B supplementation during preimplantation period with limited long-term risks","volume":"239","author":"Alfian","year":"2025","journal-title":"Theriogenology"},{"key":"B2","doi-asserted-by":"publisher","first-page":"163","DOI":"10.3390\/ijms27010163","article-title":"How early-life programming during embryogenesis imprints cellular memory","volume":"27","author":"Alfian","year":"2026","journal-title":"Int. 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