{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T06:00:59Z","timestamp":1790834459388,"version":"4.1.0"},"reference-count":31,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Cell Dev. Biol."],"abstract":"<jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>Palatal shelf elevation requires coordinated mesenchymal remodeling, but the relationship between actin organization, mechanosensitive transcription, and mesenchymal motility remains incompletely defined. This study examined how actin disruption relates to YAP\/TAZ\u2010responsive transcription and palatal mesenchymal behavior.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Primary mouse embryonic palatal mesenchymal cells and E13.5 palate organ cultures were treated with latrunculin A (Lat\u2010A). Actin organization, YAP localization, wound closure, and palatal shelf orientation were assessed. GA\u2010017, a LATS1\/2 inhibitor that can activate both YAP and TAZ, was used to examine responses in the Lat\u2010A background. RNA sequencing and reanalysis of public single\u2010cell multiomic and spatial transcriptomic datasets characterized the associated transcriptional programs.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Lat\u2010A disrupted filamentous actin, reduced wound closure, and altered YAP localization. Reduced wound closure persisted in the presence of mitomycin. GA\u2010017 partially opposed the Lat\u2010A\u2010associated changes in wound closure and transcription. RNA sequencing identified 674 genes with opposite-direction changes in the Lat\u2010A\u2010versus-control and Lat\u2010A\u2010plus\u2010GA\u2010017\u2010versus\u2010Lat\u2010A comparisons, including Ccn1, Ccn2, Ankrd1, and Amotl2. In organ culture, Lat\u2010A was associated with reduced F\u2010actin, YAP, CCN1, and CCN2 signals and abnormal palatal shelf orientation. Public datasets provided complementary evidence for the developmental expression and spatial overlap of YAP\/TEAD\u2010, CCN\u2010, actin\u2010, and migration-related programs.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Discussion<\/jats:title>\n                    <jats:p>These findings link actin disruption to altered LATS\u2010sensitive YAP\/TAZ\u2010responsive transcription and reduced palatal mesenchymal wound closure during palatal elevation. CCN1 and CCN2 are candidate components of this response.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.3389\/fcell.2026.1962374","type":"journal-article","created":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T05:40:04Z","timestamp":1790833204000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Actin disruption is associated with altered YAP\/TAZ-responsive transcription and reduced migration during palatal shelf elevation"],"prefix":"10.3389","volume":"14","author":[{"given":"Yang","family":"Bai","sequence":"first","affiliation":[{"name":"Chongqing Key Laboratory of Oral Diseases and Biomedical Sciences, Chongqing Municipal Key Laboratory of Oral Biomedical Engineering of Higher Education, Stomatological Hospital of Chongqing Medical University","place":["Chongqing, China"]},{"name":"Chongqing Technology Innovation Center of Smart Dental Medical Devices","place":["Chongqing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiarong","family":"Liu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Dental Maxillofacial Reconstruction and Biological Intelligence Manufacturing, School of Stomatology, Lanzhou University","place":["Lanzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Siqi","family":"Tang","sequence":"additional","affiliation":[{"name":"Chongqing Key Laboratory of Oral Diseases and Biomedical Sciences, Chongqing Municipal Key Laboratory of Oral Biomedical Engineering of Higher Education, Stomatological Hospital of Chongqing Medical University","place":["Chongqing, China"]},{"name":"Chongqing Technology Innovation Center of Smart Dental Medical Devices","place":["Chongqing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuxin","family":"Zhang","sequence":"additional","affiliation":[{"name":"Chongqing Key Laboratory of Oral Diseases and Biomedical Sciences, Chongqing Municipal Key Laboratory of Oral Biomedical Engineering of Higher Education, Stomatological Hospital of Chongqing Medical University","place":["Chongqing, China"]},{"name":"Chongqing Technology Innovation Center of Smart Dental Medical Devices","place":["Chongqing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chang","family":"Liu","sequence":"additional","affiliation":[{"name":"Chongqing Key Laboratory of Oral Diseases and Biomedical Sciences, Chongqing Municipal Key Laboratory of Oral Biomedical Engineering of Higher Education, Stomatological Hospital of Chongqing Medical University","place":["Chongqing, China"]},{"name":"Chongqing Technology Innovation Center of Smart Dental Medical Devices","place":["Chongqing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fangchun","family":"Chen","sequence":"additional","affiliation":[{"name":"Chongqing Key Laboratory of Oral Diseases and Biomedical Sciences, Chongqing Municipal Key Laboratory of Oral Biomedical Engineering of Higher Education, Stomatological Hospital of Chongqing Medical University","place":["Chongqing, China"]},{"name":"Innovation and Transformation of Dental Medical Devices Engineering Research Center of Chongqing Education Commission of China","place":["Chongqing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoming","family":"Wang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Dental Maxillofacial Reconstruction and Biological Intelligence Manufacturing, School of Stomatology, Lanzhou University","place":["Lanzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoxiao","family":"Pang","sequence":"additional","affiliation":[{"name":"Chongqing Key Laboratory of Oral Diseases and Biomedical Sciences, Chongqing Municipal Key Laboratory of Oral Biomedical Engineering of Higher Education, Stomatological Hospital of Chongqing Medical University","place":["Chongqing, China"]},{"name":"Innovation and Transformation of Dental Medical Devices Engineering Research Center of Chongqing Education Commission of China","place":["Chongqing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,10,1]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"1213668","DOI":"10.3389\/fphys.2023.1213668","article-title":"Actin polymerization and depolymerization in developing vertebrates","volume":"14","author":"Bai","year":"2023","journal-title":"Front. 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