{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T06:00:06Z","timestamp":1790316006312,"version":"4.1.0"},"reference-count":30,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T00:00:00Z","timestamp":1790294400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Cell Dev. Biol."],"abstract":"<jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>Inflammatory bowel disease (IBD) is characterized by chronic inflammation and disruption of intestinal integrity. This study investigates the therapeutic effects of Maggot-Derived Bioactive Peptides (BPM) in alleviating IBD symptoms and restoring intestinal function.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Using an IBD model, we assessed the impact of BPM on inflammatory responses, intestinal morphology, goblet cell density, and tight junction protein expression. Serum levels of inflammatory and oxidative stress markers were also measured to evaluate systemic effects.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>BPM significantly alleviated inflammatory responses in IBD, as evidenced by decreased levels of pro-inflammatory cytokines, including TNF-\u03b1. Treatment with BPM enhanced intestinal morphology and increased goblet cell density, promoting mucosal barrier function. Furthermore, BPM regulated p-p65 and TNF-\u03b1 pathways, effectively mitigating inflammation. Serum analysis revealed that BPM modulated the expression of inflammatory and oxidative stress markers. Importantly, BPM preserved intestinal wall integrity by upregulating tight junction proteins ZO-1, occludin, and claudin-1.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Discussion<\/jats:title>\n                    <jats:p>Our findings suggest that BPM is a promising therapeutic agent for IBD, exerting anti-inflammatory effects, enhancing intestinal morphology, and preserving epithelial integrity through multiple mechanisms. Further investigations are warranted to explore BPM\u2019s potential as a standard treatment option for IBD.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.3389\/fcell.2026.1976594","type":"journal-article","created":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T05:33:33Z","timestamp":1790314413000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Therapeutic potential of maggot-derived bioactive peptides in modulating inflammatory responses in mice model of inflammatory bowel disease"],"prefix":"10.3389","volume":"14","author":[{"given":"Lina","family":"Zhou","sequence":"first","affiliation":[{"name":"Department of Surgery, Zhangjiagang TCM Hospital Affiliated to Nanjing University of Chinese Medicine","place":["Zhangjiagang, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Surgery, Zhangjiagang TCM Hospital Affiliated to Nanjing University of Chinese Medicine","place":["Zhangjiagang, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mingfeng","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Surgery, Zhangjiagang TCM Hospital Affiliated to Nanjing University of Chinese Medicine","place":["Zhangjiagang, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mei","family":"Wu","sequence":"additional","affiliation":[{"name":"The Affiliated Taizhou People\u2019s Hospital of Nanjing Medical University","place":["Taizhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haojie","family":"Deng","sequence":"additional","affiliation":[{"name":"The Affiliated Zhangye People\u2019s Hospital of Hexi University","place":["Zhangye, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhiwei","family":"Miao","sequence":"additional","affiliation":[{"name":"Department of Surgery, Zhangjiagang TCM Hospital Affiliated to Nanjing University of Chinese Medicine","place":["Zhangjiagang, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoyu","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Surgery, Zhangjiagang TCM Hospital Affiliated to Nanjing University of Chinese Medicine","place":["Zhangjiagang, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chunyu","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Surgery, Zhangjiagang TCM Hospital Affiliated to Nanjing University of Chinese Medicine","place":["Zhangjiagang, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ke","family":"Hua","sequence":"additional","affiliation":[{"name":"Center of Reproductive Medicine, Jiaxing Maternity and Child Healthcare Hospital, College of Medicine, Jiaxing University","place":["Jiaxing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chanchan","family":"Guan","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology, Jiangsu Province TCM Hospital Affiliated to Nanjing University of Chinese Medicine","place":["Nanjing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingjing","family":"Cao","sequence":"additional","affiliation":[{"name":"Department of Surgery, Zhangjiagang TCM Hospital Affiliated to Nanjing University of Chinese Medicine","place":["Zhangjiagang, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,9,25]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"1126579","DOI":"10.3389\/fnut.2023.1126579","article-title":"Recent updates on correlation between reactive oxygen species and synbiotics for effective management of ulcerative colitis","volume":"10","author":"Ashique","year":"2023","journal-title":"Front. Nutr."},{"key":"B2","doi-asserted-by":"publisher","first-page":"1754","DOI":"10.3945\/jn.115.211441","article-title":"Saturated and unsaturated fatty acids differently modulate colonic goblet cells in vitro and in rat pups","volume":"145","author":"Benoit","year":"2015","journal-title":"J. Nutr."},{"key":"B3","doi-asserted-by":"publisher","first-page":"4804","DOI":"10.1038\/s41467-022-32505-8","article-title":"Mannose ameliorates experimental colitis by protecting intestinal barrier integrity","volume":"13","author":"Dong","year":"2022","journal-title":"Nat. Commun."},{"key":"B4","doi-asserted-by":"publisher","first-page":"108824","DOI":"10.1016\/j.cbi.2019.108824","article-title":"Beneficial role of insect-derived bioactive components against inflammation and its associated complications (colitis and arthritis) and cancer","volume":"313","author":"Dutta","year":"2019","journal-title":"Chem. Biol. Interact."},{"key":"B5","doi-asserted-by":"publisher","first-page":"20","DOI":"10.1016\/j.disamonth.2017.07.001","article-title":"A comprehensive review and update on crohn's disease","volume":"64","author":"Gajendran","year":"2018","journal-title":"Dis. Mon."},{"key":"B6","doi-asserted-by":"publisher","first-page":"1137","DOI":"10.1053\/j.gastro.2023.02.030","article-title":"Stiffness restricts the stemness of the intestinal stem cells and skews their differentiation toward goblet cells","volume":"164","author":"He","year":"2023","journal-title":"Gastroenterology"},{"key":"B7","doi-asserted-by":"publisher","first-page":"126","DOI":"10.3390\/pathogens8030126","article-title":"Alteration of gut microbiota in inflammatory bowel disease (IBD): cause or consequence? IBD treatment targeting the gut microbiome","volume":"8","author":"Khan","year":"2019","journal-title":"Pathogens"},{"key":"B8","doi-asserted-by":"publisher","first-page":"9872","DOI":"10.3748\/wjg.v20.i29.9872","article-title":"Colorectal cancer in inflammatory bowel disease: the risk, pathogenesis, prevention and diagnosis","volume":"20","author":"Kim","year":"2014","journal-title":"World J. Gastroenterol."},{"key":"B9","doi-asserted-by":"publisher","first-page":"1147","DOI":"10.1053\/j.gastro.2021.12.282","article-title":"Twenty-first century trends in the global epidemiology of pediatric-onset inflammatory bowel disease: systematic review","volume":"162","author":"Kuenzig","year":"2022","journal-title":"Gastroenterology"},{"key":"B10","doi-asserted-by":"publisher","first-page":"96","DOI":"10.3390\/metabo13010096","article-title":"Phytochemicals and regulation of NF-kB in inflammatory bowel diseases: an overview of in vitro and in vivo effects","volume":"13","author":"Laurindo","year":"2023","journal-title":"Metabolites"},{"key":"B11","doi-asserted-by":"publisher","first-page":"11","DOI":"10.5217\/ir.2015.13.1.11","article-title":"Intestinal permeability regulation by tight junction: implication on inflammatory bowel diseases","volume":"13","author":"Lee","year":"2015","journal-title":"Intest. Res."},{"key":"B12","doi-asserted-by":"publisher","first-page":"S725","DOI":"10.1093\/ecco-jcc\/jjaa014","article-title":"Clinical pharmacology of janus kinase inhibitors in inflammatory bowel disease","volume":"14","author":"Lefevre","year":"2020","journal-title":"J. Crohns Colitis"},{"key":"B13","doi-asserted-by":"publisher","first-page":"5225","DOI":"10.7150\/thno.43716","article-title":"Parthenolide ameliorates Colon inflammation through regulating Treg\/Th17 balance in a gut microbiota-dependent manner","volume":"10","author":"Liu","year":"2020","journal-title":"Theranostics"},{"key":"B14","doi-asserted-by":"publisher","first-page":"263","DOI":"10.1016\/B978-0-12-394596-9.00009-3","article-title":"Animal models of inflammatory bowel disease","volume":"105","author":"Mizoguchi","year":"2012","journal-title":"Prog. Mol. Biol. Transl. Sci."},{"key":"B15","doi-asserted-by":"publisher","first-page":"401","DOI":"10.1111\/iwj.13046","article-title":"Survey of patients of the Tver region of Russia regarding maggots and maggot therapy","volume":"16","author":"Morozov","year":"2019","journal-title":"Int. Wound J."},{"key":"B16","doi-asserted-by":"publisher","first-page":"515","DOI":"10.1097\/01.ASW.0000695776.26946.68","article-title":"Advantages of maggot debridement therapy for chronic wounds: a bibliographic review","volume":"33","author":"Moya-L\u00f3pez","year":"2020","journal-title":"Adv. Skin. Wound Care"},{"key":"B17","doi-asserted-by":"publisher","first-page":"49","DOI":"10.1038\/s41586-019-0992-y","article-title":"Colonic epithelial cell diversity in health and inflammatory bowel disease","volume":"567","author":"Parikh","year":"2019","journal-title":"Nature"},{"key":"B18","doi-asserted-by":"publisher","first-page":"2977","DOI":"10.1016\/j.bbamcr.2016.09.012","article-title":"Activation of apoptosis signalling pathways by reactive oxygen species","volume":"1863","author":"Redza-Dutordoir","year":"2016","journal-title":"Biochim. Biophys. Acta"},{"key":"B19","doi-asserted-by":"publisher","first-page":"1526","DOI":"10.3390\/ijms24021526","article-title":"Pathophysiology of inflammatory bowel disease: innate immune system","volume":"24","author":"Saez","year":"2023","journal-title":"Int. J. Mol. Sci."},{"key":"B20","doi-asserted-by":"publisher","first-page":"1217165","DOI":"10.3389\/fendo.2023.1217165","article-title":"Oxidative stress, hormones, and effects of natural antioxidants on intestinal inflammation in inflammatory bowel disease","volume":"14","author":"Sahoo","year":"2023","journal-title":"Front. Endocrinol. (Lausanne)"},{"key":"B21","doi-asserted-by":"publisher","first-page":"221","DOI":"10.1016\/j.jare.2021.07.002","article-title":"Extracellular vesicles-mediated interaction within intestinal microenvironment in inflammatory bowel disease","volume":"37","author":"Shen","year":"2022","journal-title":"J. Adv. Res."},{"key":"B22","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1111\/mve.12397","article-title":"The maggot therapy supply chain: a review of the literature and practice","volume":"34","author":"Stadler","year":"2020","journal-title":"Med. Vet. Entomol."},{"key":"B23","doi-asserted-by":"publisher","first-page":"491","DOI":"10.1603\/me09183","article-title":"An accidental but safe and effective use of Lucilia cuprina (diptera: calliphoridae) in maggot debridement therapy in Alexandria, Egypt","volume":"47","author":"Tantawi","year":"2010","journal-title":"J. Med. Entomol."},{"key":"B24","doi-asserted-by":"publisher","first-page":"107491","DOI":"10.1016\/j.intimp.2021.107491","article-title":"Pristimerin reduces dextran sulfate sodium-induced colitis in mice by inhibiting microRNA-155","volume":"94","author":"Tian","year":"2021","journal-title":"Int. Immunopharmacol."},{"key":"B25","doi-asserted-by":"publisher","first-page":"670159","DOI":"10.3389\/fimmu.2021.670159","article-title":"Therapeutic targeting of Nrf2 signaling by maggot extracts ameliorates inflammation-associated intestinal fibrosis in chronic DSS-induced colitis","volume":"12","author":"Wang","year":"2021","journal-title":"Front. Immunol."},{"key":"B26","doi-asserted-by":"publisher","first-page":"115001","DOI":"10.1016\/j.jep.2022.115001","article-title":"Ganluyin ameliorates DSS-Induced ulcerative colitis by inhibiting the enteric-origin LPS\/TLR4\/NF-\u03baB pathway","volume":"289","author":"Xiong","year":"2022","journal-title":"J. Ethnopharmacol."},{"key":"B27","doi-asserted-by":"publisher","first-page":"1052","DOI":"10.1111\/febs.17269","article-title":"A high cholesterol diet aggravates experimental colitis through SREBP2-modulated endocytosis and degradation of occludin and Zo-1 proteins","volume":"292","author":"Yang","year":"2024","journal-title":"FEBS J"},{"key":"B28","doi-asserted-by":"publisher","first-page":"1500775","DOI":"10.3389\/fcvm.2024.1500775","article-title":"Perspective: pathological transdifferentiation-a novel therapeutic target for cardiovascular diseases and chronic inflammation","volume":"11","author":"Yang","year":"2024","journal-title":"Front. Cardiovasc. Med."},{"key":"B29","doi-asserted-by":"publisher","first-page":"142","DOI":"10.1186\/s10020-022-00573-7","article-title":"Icaritin inhibits neuroinflammation in a rat cerebral ischemia model by regulating microglial polarization through the GPER-ERK-NF-\u03baB signaling pathway","volume":"28","author":"Yu","year":"2022","journal-title":"Mol. Med."},{"key":"B30","doi-asserted-by":"publisher","first-page":"15334","DOI":"10.1021\/acs.jafc.4c03764","article-title":"AQP1 deficiency drives phthalate-induced epithelial barrier disruption through intestinal inflammation","volume":"72","author":"Zhao","year":"2024","journal-title":"J. Agric. Food Chem."}],"container-title":["Frontiers in Cell and Developmental Biology"],"original-title":[],"link":[{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fcell.2026.1976594\/full","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T05:33:34Z","timestamp":1790314414000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fcell.2026.1976594\/full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,25]]},"references-count":30,"alternative-id":["10.3389\/fcell.2026.1976594"],"URL":"https:\/\/doi.org\/10.3389\/fcell.2026.1976594","relation":{},"ISSN":["2296-634X"],"issn-type":[{"value":"2296-634X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,25]]},"article-number":"1976594"}}