{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,31]],"date-time":"2026-10-31T03:56:59Z","timestamp":1793419019930,"version":"4.5.0"},"reference-count":55,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2022,2,7]],"date-time":"2022-02-07T00:00:00Z","timestamp":1644192000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2021YFF0602801"],"award-info":[{"award-number":["2021YFF0602801"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Chem."],"abstract":"<jats:p><jats:italic>Legionella<\/jats:italic>, a waterborne pathogen, is the main cause of Legionnaires\u2019 disease. Therefore, timely and accurate detection and differentiation of <jats:italic>Legionella pneumophila<\/jats:italic> and non<jats:italic>-Legionella pneumophila<\/jats:italic> species is crucial. In this study, we develop an easy and rapid recombinase polymerase amplification assay combined with EuNPs-based lateral flow immunochromatography (EuNPs-LFIC-RPA) to specifically distinguish <jats:italic>Legionella pneumophila<\/jats:italic> and non<jats:italic>-Legionella pneumophila<\/jats:italic>. We designed primers based on the <jats:italic>mip<\/jats:italic> gene of <jats:italic>Legionella pneumophila<\/jats:italic> and the 5S rRNA gene of non-<jats:italic>Legionella pneumophila.<\/jats:italic> The recombinase polymerase amplification reaction could go to completion in 10\u00a0min at 37\u00b0C, and the amplification products could be detected within 5\u00a0min with EuNPs-LFIC strips. Using a florescent test strip reader, the quantitative results were achieved by reading the colored signal intensities on the strips. The sensitivity was 1.6 \u00d7 10<jats:sup>1<\/jats:sup>\u00a0CFU\/ml, and a linear standard linear curve plotted from the test strip reader had a correlation coefficient for the determination of <jats:italic>Legionella pneumophila<\/jats:italic> (<jats:italic>R<\/jats:italic><jats:sup>2<\/jats:sup> = 0.9516). Completed concordance for the presence or absence of <jats:italic>Legionella pneumophila<\/jats:italic> by EuNPs-LFIC-RPA and qPCR was 97.32% (<jats:italic>\u03ba<\/jats:italic> = 0.79, 95% CI), according to an analysis of practical water samples (<jats:italic>n<\/jats:italic> = 112). In short, this work shows the feasibility of EuNPs-LFIC-RPA for efficient and rapid monitoring of <jats:italic>Legionella pneumophila<\/jats:italic> and non-<jats:italic>Legionella pneumophila<\/jats:italic> in water samples.<\/jats:p>","DOI":"10.3389\/fchem.2021.815189","type":"journal-article","created":{"date-parts":[[2022,2,7]],"date-time":"2022-02-07T08:47:46Z","timestamp":1644223666000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":6,"title":["Rapid Detection and Differentiation of Legionella pneumophila and Non-Legionella pneumophila Species by Using Recombinase Polymerase Amplification Combined With EuNPs-Based Lateral Flow Immunochromatography"],"prefix":"10.3389","volume":"9","author":[{"given":"Jungang","family":"Du","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Biao","family":"Ma","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiali","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yaping","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tianyu","family":"Dou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shujuan","family":"Xu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mingzhou","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2022,2,7]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"15972","DOI":"10.1038\/s41598-018-34275-0","article-title":"Genome-Informed Diagnostics for Specific and Rapid Detection of Pectobacterium Species Using Recombinase Polymerase Amplification Coupled with a Lateral Flow Device","volume":"8","author":"Ahmed","year":"2018","journal-title":"Sci. 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