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Chem."],"abstract":"<jats:p>Furunculosis, which is caused by <jats:italic>Aeromonas salmonicida<\/jats:italic>, can induce septicemia, leading to the rapid death of fishes belonging to Salmonidae, Cyprinidae, and Fuscheridae, and lamprey. Targeting <jats:italic>A. salmonicida,<\/jats:italic> five DNAzyme sequences with the highest enrichment rates were selected through the Systematic Evolution of Ligands by Exponential Enrichment (SELEX). The enrichment rates were 34.78, 23.60, 8.91, 2.89, and 2.34%, respectively. The DNAzyme with the highest activity, named D-AS-2, showed specificity and sensitivity. D-AS-2 was combined with carboxyl-functionalized graphene to construct a biosensor, which showed good fluorescence response to scabies lesion samples. The diagnostic procedure was completed in &amp;lt;2\u00a0min and can be used for the on-site diagnosis of fish diseases. 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