{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,31]],"date-time":"2025-12-31T18:02:27Z","timestamp":1767204147286,"version":"build-2238731810"},"reference-count":15,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2025,7,15]],"date-time":"2025-07-15T00:00:00Z","timestamp":1752537600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Chem."],"abstract":"<jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>Psoriasis is a chronic immune-mediated inflammatory skin disease. Xiaoyin Jiedu Decoction (XYJDY) is a traditional Chinese medicinal formula that has demonstrated significant clinical efficacy in alleviating psoriatic symptoms; however, its underlying pharmacological mechanisms remain unclear.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      We employed network pharmacology, machine learning\u2013based target screening, and functional enrichment to identify key targets and pathways. Single-cell RNA sequencing (scRNA-seq) and spatial transcriptomics (ST) were used to validate gene expression. An IL-17A\u2013induced HaCaT cell model was established for\n                      <jats:italic>in vitro<\/jats:italic>\n                      validation.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      AKR1B10 was identified as the core therapeutic target of XYJDY in psoriasis. It was markedly upregulated in psoriatic skin lesions, primarily enriched in keratinocytes, and its expression demonstrated positive correlations with multiple pro-inflammatory immune cell subsets.\n                      <jats:italic>In vitro<\/jats:italic>\n                      experiments showed that XYJDY-medicated serum significantly downregulated AKR1B10 expression in IL\u201317A\u2013stimulated HaCaT cells.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>This study reveals that the multi-component formula XYJDY exerts anti-psoriatic effects through a multi-target synergistic mechanism, in which AKR1B10 is a potential core target. These findings provide a theoretical foundation for further exploration of the molecular mechanisms underlying the efficacy of XYJDY in psoriasis treatment.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.3389\/fchem.2025.1623449","type":"journal-article","created":{"date-parts":[[2025,7,15]],"date-time":"2025-07-15T05:25:47Z","timestamp":1752557147000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Investigation of the mechanism of Xiaoyin Jiedu Yin in the treatment of psoriasis based on bioinformatics, machine learning"],"prefix":"10.3389","volume":"13","author":[{"given":"Ru-Nan","family":"Fang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yang","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yang","family":"Shen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuan","family":"Sun","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jian-Hong","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1965","published-online":{"date-parts":[[2025,7,15]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"1945","DOI":"10.1001\/jama.2020.4006","article-title":"Pathophysiology, clinical presentation, and treatment of psoriasis: a review","volume":"323","author":"Armstrong","year":"2020","journal-title":"JAMA"},{"key":"B2","doi-asserted-by":"publisher","first-page":"2332","DOI":"10.1158\/0008-5472.CAN-17-2445","article-title":"IRAK1 augments cancer stemness and drug resistance via the AP-1\/AKR1B10 signaling cascade in hepatocellular carcinoma","volume":"78","author":"Cheng","year":"2018","journal-title":"Cancer Res."},{"key":"B3","doi-asserted-by":"publisher","first-page":"332","DOI":"10.3390\/metabo11060332","article-title":"The role of AKR1B10 in physiology and pathophysiology","volume":"11","author":"Endo","year":"2021","journal-title":"Metabolites"},{"key":"B4","doi-asserted-by":"publisher","first-page":"56","DOI":"10.3390\/pharmaceutics17010056","article-title":"Pathophysiology and treatment of psoriasis: from clinical practice to basic research","volume":"17","author":"Gao","year":"2025","journal-title":"Pharmaceutics"},{"key":"B5","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1155\/2017\/8717369","article-title":"Combined transcriptomic analysis revealed AKR1B10 played an important role in psoriasis through the dysregulated lipid pathway and overproliferation of keratinocyte","volume":"2017","author":"Gao","year":"2017","journal-title":"BioMed Res. Int."},{"key":"B6","doi-asserted-by":"publisher","first-page":"e13390","DOI":"10.1111\/sji.13390","article-title":"Role of AKR1B10 in inflammatory diseases","volume":"100","author":"Guo","year":"2024","journal-title":"Scand. J. Immunol."},{"key":"B7","doi-asserted-by":"publisher","first-page":"773","DOI":"10.4143\/crt.2020.974","article-title":"LASSO-based machine learning algorithm for prediction of lymph node metastasis in T1 colorectal cancer","volume":"53","author":"Kang","year":"2021","journal-title":"Cancer Res. Treat."},{"key":"B8","doi-asserted-by":"publisher","first-page":"e39957","DOI":"10.1016\/j.heliyon.2024.e39957","article-title":"JUN and ATF3 in Gout: ferroptosis-related potential diagnostic biomarkers","volume":"10","author":"Li","year":"2024","journal-title":"Heliyon"},{"key":"B9","doi-asserted-by":"publisher","first-page":"50","DOI":"10.1186\/s13020-024-00924-y","article-title":"TCMNPAS: a comprehensive analysis platform integrating network formulaology and network pharmacology for exploring traditional Chinese medicine","volume":"19","author":"Liu","year":"2024","journal-title":"Chin. Med."},{"key":"B10","doi-asserted-by":"publisher","first-page":"e70539","DOI":"10.1096\/fj.202500003R","article-title":"MiR\u00b7101 and miR\u00b7122 targeting \u03b4-catenin to regulate keratinocyte responsiveness to IL-17a in psoriasis","volume":"39","author":"Shen","year":"2025","journal-title":"FASEB J. Official Publ. Fed. Am. Soc. Exp. Biol."},{"key":"B11","doi-asserted-by":"publisher","first-page":"138","DOI":"10.1186\/s13020-021-00550-y","article-title":"A review of Chinese medicine for the treatment of psoriasis: principles, methods and analysis","volume":"16","author":"Su","year":"2021","journal-title":"Chin. Med."},{"key":"B12","doi-asserted-by":"publisher","first-page":"4","DOI":"10.3390\/pharmaceutics8010004","article-title":"Microarray analysis of differentially-expressed genes encoding CYP450 and phase II drug metabolizing enzymes in psoriasis and melanoma","volume":"8","author":"Sumantran","year":"2016","journal-title":"Pharmaceutics"},{"key":"B13","doi-asserted-by":"publisher","first-page":"377","DOI":"10.1016\/j.jaad.2016.07.064","article-title":"Psoriasis and comorbid diseases: epidemiology","volume":"76","author":"Takeshita","year":"2017","journal-title":"J. Am. Acad. Dermatology"},{"key":"B14","doi-asserted-by":"publisher","first-page":"116306","DOI":"10.1016\/j.jep.2023.116306","article-title":"Network pharmacology, a promising approach to reveal the pharmacology mechanism of Chinese medicine formula","volume":"309","author":"Zhao","year":"2023","journal-title":"J. Ethnopharmacol."},{"key":"B15","doi-asserted-by":"publisher","first-page":"177","DOI":"10.1016\/j.ncrna.2024.09.003","article-title":"Study on molecular mechanism of intervertebral disc degeneration by single cell hdWGCNA combined with transcriptome sequencing","volume":"10","author":"Zhao","year":"2025","journal-title":"Non-Coding RNA Res."}],"updated-by":[{"DOI":"10.3389\/fchem.2025.1685169","type":"correction","label":"Correction","source":"publisher","updated":{"date-parts":[[2025,9,17]],"date-time":"2025-09-17T00:00:00Z","timestamp":1758067200000}}],"container-title":["Frontiers in Chemistry"],"original-title":[],"link":[{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fchem.2025.1623449\/full","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,3]],"date-time":"2025-10-03T11:28:30Z","timestamp":1759490910000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fchem.2025.1623449\/full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,7,15]]},"references-count":15,"alternative-id":["10.3389\/fchem.2025.1623449"],"URL":"https:\/\/doi.org\/10.3389\/fchem.2025.1623449","relation":{},"ISSN":["2296-2646"],"issn-type":[{"value":"2296-2646","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,7,15]]},"article-number":"1623449"}}