{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,23]],"date-time":"2026-08-23T16:29:59Z","timestamp":1787502599311,"version":"build-2736575974"},"reference-count":21,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,1,21]],"date-time":"2026-01-21T00:00:00Z","timestamp":1768953600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Chem."],"abstract":"<jats:p>\n                    Non-alcoholic fatty liver disease (NAFLD) represents a chronic liver disorder with widespread global prevalence, primarily attributed to hepatic lipid accumulation, oxidative stress, and inflammatory responses. In the realm of traditional Chinese medicine,\n                    <jats:italic>Ganoderma lucidum<\/jats:italic>\n                    is predominantly utilized for its hepatoprotective properties. The objective of this study was to isolate and identify novel bioactive compounds capable of mitigating hepatic fat accumulation. An\n                    <jats:italic>in vitro<\/jats:italic>\n                    steatosis model was established using oleic acid-induced HepG2 cells to evaluate the total triglyceride (TG) content across various components. Fractionation of the compounds was guided by the observed reduction in TG content, employing multiple chromatographic techniques to successfully isolate ten Ganoderma triterpenes. Structural elucidation was achieved through 1D and 2D NMR spectroscopy, supplemented by additional spectroscopic methods. This investigation led to the identification of two previously unreported lanostane-type triterpenes (1\u20132) alongside eight known analogues (3\u201310). Compound 1 and 2 exhibit structural distinctions from the other compounds, primarily in the substituents at positions C-3, C-7, and C-15, as well as in the spatial orientation of these substituents.\n                    <jats:italic>In vitro<\/jats:italic>\n                    experiments were conducted to assess the efficacy of various compounds in inhibiting lipid accumulation. Compound 1-5 demonstrated a significant reduction in TG levels within the OA-induced HepG2 cell model (\n                    <jats:italic>p<\/jats:italic>\n                    &amp;lt; 0.05). In comparison to the model group, Compound 1 demonstrated a moderate lipid-lowering effect, (2.11\u00a0mmol\/gprot vs. 2.70\u00a0mmol\/gprot,\n                    <jats:italic>p<\/jats:italic>\n                    &amp;lt; 0.003). Conversely, Compound 2 exhibited a significantly more pronounced lipid-lowering effect, (1.27\u00a0mmol\/gprot vs. 2.70\u00a0mmol\/gprot,\n                    <jats:italic>p<\/jats:italic>\n                    &amp;lt; 0.0001). Furthermore, when compared with the positive control drug, the lipid-lowering efficacy of Compound 2 was significantly superior to that of Compound 1. Furthermore, the application of network pharmacology, molecular docking, and molecular dynamics simulations elucidated the mechanism of action underlying the effects of methyl ganoderenic acid A(2).\n                  <\/jats:p>","DOI":"10.3389\/fchem.2025.1726447","type":"journal-article","created":{"date-parts":[[2026,1,21]],"date-time":"2026-01-21T05:36:34Z","timestamp":1768973794000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":2,"title":["A new lanostane-type triterpene with lipid-lowering activity from Ganoderma lucidum and an additional analogue"],"prefix":"10.3389","volume":"13","author":[{"given":"Xuesheng","family":"Hu","sequence":"first","affiliation":[{"name":"Jilin Ginseng Academy, Changchun University of Chinese Medicine","place":["Changchun, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lu","family":"Han","sequence":"additional","affiliation":[{"name":"Jilin Ginseng Academy, Changchun University of Chinese Medicine","place":["Changchun, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yiying","family":"Tan","sequence":"additional","affiliation":[{"name":"Jilin Ginseng Academy, Changchun University of Chinese Medicine","place":["Changchun, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peitong","family":"Wu","sequence":"additional","affiliation":[{"name":"Jilin Ginseng Academy, Changchun University of Chinese Medicine","place":["Changchun, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yajing","family":"Li","sequence":"additional","affiliation":[{"name":"Jilin Ginseng Academy, Changchun University of Chinese Medicine","place":["Changchun, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wanjie","family":"Liu","sequence":"additional","affiliation":[{"name":"Jilin Ginseng Academy, Changchun University of Chinese Medicine","place":["Changchun, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiaming","family":"Shen","sequence":"additional","affiliation":[{"name":"Jilin Ginseng Academy, Changchun University of Chinese Medicine","place":["Changchun, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yishan","family":"Li","sequence":"additional","affiliation":[{"name":"Jilin Ginseng Academy, Changchun University of Chinese Medicine","place":["Changchun, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ming","family":"Yang","sequence":"additional","affiliation":[{"name":"Jilin Ginseng Academy, Changchun University of Chinese Medicine","place":["Changchun, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chunnan","family":"Li","sequence":"additional","affiliation":[{"name":"Jilin Ginseng Academy, Changchun University of Chinese Medicine","place":["Changchun, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiaming","family":"Sun","sequence":"additional","affiliation":[{"name":"Jilin Ginseng Academy, Changchun University of Chinese Medicine","place":["Changchun, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,1,21]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"1874","DOI":"10.3390\/nu15081874","article-title":"Ganoderma lucidum: Novel insight into hepatoprotective potential with mechanisms of action","volume":"15","author":"Ahmad","year":"2023","journal-title":"Nutrients"},{"key":"B2","doi-asserted-by":"publisher","DOI":"10.38212\/2224-6614.2699","article-title":"Determination of ganoderic acids in triterpenoid constituents of Ganoderma tsugae","volume":"11","author":"Chen","year":"2020","journal-title":"J. Food Drug Anal."},{"key":"B3","doi-asserted-by":"publisher","first-page":"890","DOI":"10.1016\/j.ijbiomac.2019.11.047","article-title":"Intake of Ganoderma lucidum polysaccharides reverses the disturbed gut microbiota and metabolism in type 2 diabetic rats","volume":"155","author":"Chen","year":"2020","journal-title":"Int. J. Biol. Macromol."},{"key":"B4","doi-asserted-by":"publisher","first-page":"1524","DOI":"10.3390\/biomedicines9111524","article-title":"Genetics, immunity and nutrition boost the switching from NASH to HCC","volume":"9","author":"Dongiovanni","year":"2021","journal-title":"Biomedicines"},{"key":"B5","doi-asserted-by":"publisher","first-page":"123","DOI":"10.14218\/JCTH.2016.00010","article-title":"Nonalcoholic lipid accumulation and hepatocyte malignant transformation","volume":"4","author":"Gu","year":"2016","journal-title":"J. Clin. Transl. Hepatol."},{"key":"B6","doi-asserted-by":"publisher","first-page":"2508","DOI":"10.1002\/hep.32045","article-title":"Fatty acid synthase\u2013suppressor screening identifies sorting nexin 8 as a therapeutic target for NAFLD","volume":"74","author":"Hu","year":"2021","journal-title":"Hepatology"},{"key":"B7","doi-asserted-by":"publisher","first-page":"1582","DOI":"10.1021\/np0302293","article-title":"Lucidenic acids P and Q, methyl lucidenate P, and other triterpenoids from the fungus Ganoderma lucidum and their inhibitory effects on Epstein\u2212Barr virus activation","volume":"66","author":"Iwatsuki","year":"2003","journal-title":"J. Nat. Prod."},{"key":"B8","doi-asserted-by":"publisher","first-page":"4018","DOI":"10.1248\/cpb.34.4018","article-title":"Constituents of the fungus Ganoderma lucidum (Fr.) Karst. II structures of ganoderic acids F, G, and H, lucidenic acids D2 and E2, and related compounds","volume":"34","author":"Kikuchi","year":"1986","journal-title":"Chem. Pharm. 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Asian Nat. Prod. Res."},{"key":"B19","doi-asserted-by":"publisher","first-page":"e36987","DOI":"10.1016\/j.heliyon.2024.e36987","article-title":"Bioactivities and industrial standardization status of Ganoderma lucidum: a comprehensive review","volume":"10","author":"Wu","year":"2024","journal-title":"Heliyon"},{"key":"B20","doi-asserted-by":"publisher","first-page":"1390","DOI":"10.18632\/aging.205434","article-title":"Ganodermanontriol regulates tumor-associated M2 macrophage polarization in gastric cancer","volume":"16","author":"Zhang","year":"2024","journal-title":"Aging"},{"key":"B21","doi-asserted-by":"publisher","first-page":"66","DOI":"10.29219\/fnr.v66.8745","article-title":"Sporoderm-broken spore powder of Ganoderma lucidum ameliorate obesity and inflammation process in high-fat diet-induced obese mice","volume":"66","author":"Zhong","year":"2022","journal-title":"Food Nutr. Res."}],"container-title":["Frontiers in Chemistry"],"original-title":[],"link":[{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fchem.2025.1726447\/full","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,1,21]],"date-time":"2026-01-21T05:36:36Z","timestamp":1768973796000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fchem.2025.1726447\/full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,1,21]]},"references-count":21,"alternative-id":["10.3389\/fchem.2025.1726447"],"URL":"https:\/\/doi.org\/10.3389\/fchem.2025.1726447","relation":{},"ISSN":["2296-2646"],"issn-type":[{"value":"2296-2646","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,1,21]]},"article-number":"1726447"}}