{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T11:10:16Z","timestamp":1788952216583,"version":"build-2803163510"},"reference-count":55,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T00:00:00Z","timestamp":1788912000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Chem."],"abstract":"<jats:p>\n                    Recognition of amyloid-\u03b2 (A\u03b2) by structurally defined inorganic scaffolds offers a route to dissecting how framework geometry and surface chemistry govern peptide\u2013material interactions. We report transferrin (Tf)\/KLVFF-PCN-222, a bioinorganic recognition platform built by covalently co-grafting the A\u03b2-homologous pentapeptide KLVFF and transferrin (Tf) onto the csq-topology porphyrinic zirconium MOF PCN-222 via EDC\/NHS chemistry. The one-dimensional hexagonal mesopore channels (\u223c3.7\u00a0nm) and the dense meso-tetrakis (4-carboxyphenyl)porphyrin (TCPP) sites of the csq framework provide geometric access for A\u03b21\u201342 monomer diffusion and an array of peptide\u2013porphyrin recognition contacts. Fluorescence quenching and isothermal titration calorimetry returned an apparent dissociation constant (\n                    <jats:italic>K<\/jats:italic>\n                    <jats:sub>d,app<\/jats:sub>\n                    ) of 33.5 \u00b1 4.2\u00a0nM for the A\u03b21\u201342 monomer (apparent fluorescence-mode limit of detection (LOD) = 1.8\u00a0nM), and circular dichroism showed that the probe suppressed A\u03b2 \u03b2-sheet conversion (14% vs. 41% \u03b2-sheet content at 24\u00a0h). A panel of structural controls\u2014scrambled FFKLV, the narrow-pore fcu MOF UiO-66-NH\n                    <jats:sub>2<\/jats:sub>\n                    , and the TCPP-based ftw MOF-525\u2014together with a selectivity screen against BSA, IgG, Tau, \u03b1-synuclein, and lysozyme (selectivity ratio &amp;gt;12 for A\u03b21\u201342) indicates that KLVFF sequence specificity and csq mesopore geometry act cooperatively to drive recognition. The framework retains an intrinsic NIR photothermal property (photothermal conversion efficiency, \u03b7 = 47.3 \u00b1 1.8% at 808 nm); the photoacoustic measurement reported here is a probe concentration calibration only, and no A\u03b2-responsive photoacoustic signal was established. In an hCMEC\/D3\n                    <jats:italic>in vitro<\/jats:italic>\n                    monolayer, the apparent permeability coefficient (\n                    <jats:italic>P<\/jats:italic>\n                    <jats:sub>app<\/jats:sub>\n                    ) reached 1.78 \u00d7 10\n                    <jats:sup>\u22125<\/jats:sup>\n                    cm s\n                    <jats:sup>\u22121<\/jats:sup>\n                    with \u223c48% of the flux being TfR1-competition-sensitive, and SH-SY5Y viability was partially preserved in a tandem BBB\u2013neuron Transwell model. All validations are\n                    <jats:italic>in vitro<\/jats:italic>\n                    . This work provides a topology-controlled, structure\u2013function proof of concept for csq Zr-TCPP frameworks as bioinorganic platforms for A\u03b2 recognition and conformational modulation.\n                  <\/jats:p>","DOI":"10.3389\/fchem.2026.1906393","type":"journal-article","created":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T10:16:42Z","timestamp":1788949002000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Topology- and sequence-controlled \u03b2-amyloid recognition by a KLVFF\/transferrin-functionalized csq porphyrinic zirconium MOF (PCN-222)"],"prefix":"10.3389","volume":"14","author":[{"given":"Xiao","family":"Cheng","sequence":"first","affiliation":[{"name":"Department of Neurology, Shanxi Provincial People\u2019s Hospital, Shanxi Medical University","place":["Taiyuan, China"]},{"name":"Shanxi Key Laboratory of Neurobehavior and Brain Disease Control","place":["Taiyuan, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongen","family":"Wei","sequence":"additional","affiliation":[{"name":"Department of Neurology, Shanxi Provincial People\u2019s Hospital, Shanxi Medical University","place":["Taiyuan, China"]},{"name":"Shanxi Key Laboratory of Neurobehavior and Brain Disease Control","place":["Taiyuan, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,9,9]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"3708","DOI":"10.1002\/alz.13809","article-title":"2024 Alzheimer\u2019s disease facts and figures","volume":"20","year":"2024","journal-title":"Alzheimers Dement."},{"key":"B2","doi-asserted-by":"publisher","first-page":"13850","DOI":"10.1021\/ja8057953","article-title":"A new zirconium inorganic building brick forming metal organic frameworks with exceptional stability","volume":"130","author":"Cavka","year":"2008","journal-title":"J. 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