{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T01:30:22Z","timestamp":1791423022097,"version":"4.3.3"},"reference-count":52,"publisher":"Frontiers Media SA","content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Cell. Infect. Microbiol."],"DOI":"10.3389\/fcimb.2015.00070","type":"journal-article","created":{"date-parts":[[2015,10,1]],"date-time":"2015-10-01T08:23:09Z","timestamp":1443687789000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":38,"title":["The icmF3 locus is involved in multiple adaptation- and virulence-related characteristics in Pseudomonas aeruginosa PAO1"],"prefix":"10.3389","volume":"5","author":[{"given":"Jinshui","family":"Lin","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juanli","family":"Cheng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Keqi","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chenghao","family":"Guo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weipeng","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xu","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Ding","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li","family":"Ma","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yao","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xihui","family":"Shen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2015,10,1]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"884","DOI":"10.1016\/j.cell.2013.01.042","article-title":"Tit-for-tat: type VI secretion system counterattack during bacterial cell-cell interactions","volume":"152","author":"Basler","year":"2013","journal-title":"Cell"},{"key":"B2","doi-asserted-by":"crossref","first-page":"948","DOI":"10.2144\/00295bm04","article-title":"Integration-proficient Pseudomonas aeruginosa vectors for isolation of single-copy chromosomal lacZ and lux gene fusions","volume":"29","author":"Becher","year":"2000","journal-title":"Biotechniques"},{"key":"B3","doi-asserted-by":"publisher","first-page":"534","DOI":"10.1016\/j.ijmm.2010.08.005","article-title":"Protein secretion systems in Pseudomonas aeruginosa: a wealth of pathogenic weapons","volume":"300","author":"Bleves","year":"2010","journal-title":"Int. J. Med. Microbiol."},{"key":"B4","doi-asserted-by":"publisher","first-page":"63","DOI":"10.1126\/science.1260064","article-title":"The type VI secretion system of Vibrio cholerae fosters horizontal gene transfer","volume":"347","author":"Borgeaud","year":"2015","journal-title":"Science"},{"key":"B5","doi-asserted-by":"publisher","first-page":"1337","DOI":"10.1099\/00221287-138-7-1337","article-title":"Stability, frequency and multiplicity of transposon insertions in the pyoverdine region in the chromosomes of different fluorescent pseudomonads","volume":"138","author":"Cornelis","year":"1992","journal-title":"J. Gen. Microbiol."},{"issue":"75","key":"B6","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2013.00075","article-title":"Pseudomonas aeruginosa adapts its iron uptake strategies in function of the type of infections","volume":"3","author":"Cornelis","year":"2013","journal-title":"Front. Cell. Infect. Microbiol."},{"key":"B7","doi-asserted-by":"publisher","first-page":"922","DOI":"10.1016\/S0006-291X(02)00782-9","article-title":"Involvement of in vivo induced icmF gene of Vibrio cholerae in motility, adherence to epithelial cells, and conjugation frequency","volume":"295","author":"Das","year":"2002","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"B8","doi-asserted-by":"publisher","first-page":"2954","DOI":"10.1099\/mic.0.050005-0","article-title":"Characterization of IcmF of the type VI secretion system in an avian pathogenic Escherichia coli (APEC) strain","volume":"157","author":"de Pace","year":"2011","journal-title":"Microbiology"},{"key":"B9","doi-asserted-by":"publisher","first-page":"29","DOI":"10.1046\/j.1365-2958.2003.03672.x","article-title":"The Pseudomonas aeruginosa quinolone signal molecule overcomes the cell density-dependency of the quorum sensing hierarchy, regulates rhl-dependent genes at the onset of stationary phase and can be produced in the absence of LasR","volume":"50","author":"Diggle","year":"2003","journal-title":"Mol. Microbiol."},{"key":"B10","doi-asserted-by":"publisher","first-page":"2522","DOI":"10.1128\/AAC.00035-09","article-title":"Genomewide identification of genetic determinants of antimicrobial drug resistance in Pseudomonas aeruginosa","volume":"53","author":"Dotsch","year":"2009","journal-title":"Antimicrob. Agents Chemother."},{"key":"B11","doi-asserted-by":"crossref","first-page":"884","DOI":"10.1128\/jb.172.2.884-900.1990","article-title":"Identification and characterization of genes for a second anthranilate synthase in Pseudomonas aeruginosa: interchangeability of the two anthranilate synthases and evolutionary implications","volume":"172","author":"Essar","year":"1990","journal-title":"J. Bacteriol."},{"key":"B12","doi-asserted-by":"publisher","first-page":"849","DOI":"10.1111\/1574-6976.12018","article-title":"Regulation of flagellar motility during biofilm formation","volume":"37","author":"Guttenplan","year":"2013","journal-title":"FEMS Microbiol. Rev."},{"key":"B13","doi-asserted-by":"publisher","first-page":"250","DOI":"10.1126\/science.1243745","article-title":"Type 6 secretion system-mediated immunity to type 4 secretion system-mediated gene transfer","volume":"342","author":"Ho","year":"2013","journal-title":"Science"},{"key":"B14","doi-asserted-by":"publisher","first-page":"59","DOI":"10.1006\/plas.1999.1441","article-title":"Integration-proficient plasmids for Pseudomonas aeruginosa: site-specific integration and use for engineering of reporter and expression strains","volume":"43","author":"Hoang","year":"2000","journal-title":"Plasmid"},{"key":"B15","doi-asserted-by":"publisher","first-page":"1171","DOI":"10.1038\/nature03912","article-title":"Aminoglycoside antibiotics induce bacterial biofilm formation","volume":"436","author":"Hoffman","year":"2005","journal-title":"Nature"},{"key":"B16","doi-asserted-by":"publisher","first-page":"20440","DOI":"10.1073\/pnas.0908760106","article-title":"Molecular basis of pyoverdine siderophore recycling in Pseudomonas aeruginosa","volume":"106","author":"Imperi","year":"2009","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"B17","doi-asserted-by":"publisher","first-page":"81","DOI":"10.1111\/j.1574-6968.2008.01089.x","article-title":"Inhibition of swarming motility of Pseudomonas aeruginosa by branched-chain fatty acids","volume":"281","author":"Inoue","year":"2008","journal-title":"FEMS Microbiol. Lett."},{"key":"B18","doi-asserted-by":"publisher","first-page":"600","DOI":"10.1016\/j.chom.2014.04.010","article-title":"A Pseudomonas aeruginosa type VI secretion phospholipase D effector targets both prokaryotic and eukaryotic cells","volume":"15","author":"Jiang","year":"2014","journal-title":"Cell Host Microbe"},{"key":"B19","doi-asserted-by":"publisher","first-page":"567","DOI":"10.1006\/jmbi.2000.4315","article-title":"Predicting transmembrane protein topology with a hidden Markov model: application to complete genomes","volume":"305","author":"Krogh","year":"2001","journal-title":"J. Mol. Biol."},{"key":"B20","doi-asserted-by":"publisher","first-page":"333","DOI":"10.1111\/j.1365-2958.1995.mmi_17020333.x","article-title":"Multiple homologues of LuxR and LuxI control expression of virulence determinants and secondary metabolites through quorum sensing in Pseudomonas aeruginosa PAO1","volume":"17","author":"Latifi","year":"1995","journal-title":"Mol. Microbiol."},{"key":"B21","doi-asserted-by":"publisher","first-page":"199","DOI":"10.1046\/j.1365-2958.2003.03423.x","article-title":"Interactions of the quorum sensing regulator QscR: interaction with itself and the other regulators of Pseudomonas aeruginosa LasR and RhlR","volume":"48","author":"Ledgham","year":"2003","journal-title":"Mol. Microbiol."},{"key":"B22","doi-asserted-by":"publisher","first-page":"e05701","DOI":"10.7554\/eLife.05701","article-title":"Kin cell lysis is a danger signal that activates antibacterial pathways of Pseudomonas aeruginosa","volume":"4","author":"Leroux","year":"2015","journal-title":"Elife"},{"key":"B23","doi-asserted-by":"publisher","first-page":"2845","DOI":"10.1099\/mic.0.029082-0","article-title":"Quorum sensing differentially regulates Pseudomonas aeruginosa type VI secretion locus I and homologous loci II and III, which are required for pathogenesis","volume":"155","author":"Lesic","year":"2009","journal-title":"Microbiology"},{"key":"B24","doi-asserted-by":"publisher","first-page":"4316","DOI":"10.1128\/JB.00029-09","article-title":"An IcmF family protein, ImpLM, is an integral inner membrane protein interacting with ImpKL, and its walker a motif is required for type VI secretion system-mediated Hcp secretion in Agrobacterium tumefaciens","volume":"191","author":"Ma","year":"2009","journal-title":"J. Bacteriol."},{"key":"B25","doi-asserted-by":"publisher","first-page":"15610","DOI":"10.1074\/jbc.M111.301630","article-title":"IcmF family protein TssM exhibits ATPase activity and energizes type VI secretion","volume":"287","author":"Ma","year":"2012","journal-title":"J. Biol. Chem."},{"key":"B26","doi-asserted-by":"publisher","first-page":"6454","DOI":"10.1128\/JB.183.21.6454-6465.2001","article-title":"Functional analysis of genes for biosynthesis of pyocyanin and phenazine-1-carboxamide from Pseudomonas aeruginosa PAO1","volume":"183","author":"Mavrodi","year":"2001","journal-title":"J. Bacteriol."},{"key":"B27","doi-asserted-by":"publisher","first-page":"219","DOI":"10.1099\/00221287-107-2-319","article-title":"The fluorescent pigment of Pseudomonas fluorescens: biosynthesis, purification and physicochemical properties","volume":"107","author":"Meyer","year":"1978","journal-title":"Soc. General Microbiol."},{"key":"B28","volume-title":"A Short Course in Bacterial Genetics: A Laboratory Manual and Handbook for Escherichia coli and Related Bacteria","year":"1992"},{"key":"B29","doi-asserted-by":"publisher","first-page":"1526","DOI":"10.1126\/science.1128393","article-title":"A virulence locus of Pseudomonas aeruginosa encodes a protein secretion apparatus","volume":"312","author":"Mougous","year":"2006","journal-title":"Science"},{"key":"B30","doi-asserted-by":"publisher","first-page":"449","DOI":"10.1046\/j.1365-2958.1998.00797.x","article-title":"Initiation of biofilm formation in Pseudomonas fluorescens WCS365 proceeds via multiple, convergent signalling pathways: a genetic analysis","volume":"28","author":"O'Toole","year":"1998","journal-title":"Mol. Microbiol."},{"key":"B31","doi-asserted-by":"publisher","first-page":"4338","DOI":"10.1128\/IAI.73.7.4338-4345.2005","article-title":"sciS, an icmF homolog in Salmonella enterica serovar Typhimurium, limits intracellular replication and decreases virulence","volume":"73","author":"Parsons","year":"2005","journal-title":"Infect. Immun."},{"key":"B32","doi-asserted-by":"publisher","first-page":"145","DOI":"10.1186\/1471-2180-12-145","article-title":"Potential novel therapeutic strategies in cystic fibrosis: antimicrobial and anti-biofilm activity of natural and designed alpha-helical peptides against Staphylococcus aureus, Pseudomonas aeruginosa, and Stenotrophomonas maltophilia","volume":"12","author":"Pompilio","year":"2012","journal-title":"BMC Microbiol."},{"key":"B33","doi-asserted-by":"publisher","first-page":"e1685","DOI":"10.1371\/journal.pone.0001685","article-title":"Fitness of isogenic colony morphology variants of Pseudomonas aeruginosa in murine airway infection","volume":"3","author":"Rakhimova","year":"2008","journal-title":"PLoS ONE"},{"key":"B34","doi-asserted-by":"publisher","first-page":"508","DOI":"10.1038\/nature12074","article-title":"Diverse type VI secretion phospholipases are functionally plastic antibacterial effectors","volume":"496","author":"Russell","year":"2013","journal-title":"Nature"},{"key":"B35","doi-asserted-by":"publisher","first-page":"137","DOI":"10.1038\/nrmicro3185","article-title":"Type VI secretion system effectors: poisons with a purpose","volume":"12","author":"Russell","year":"2014","journal-title":"Nat. Rev. Microbiol."},{"key":"B36","first-page":"406","article-title":"The neighbor-joining method: a new method for reconstructing phylogenetic trees","volume":"4","author":"Saitou","year":"1987","journal-title":"Mol. Biol. Evol."},{"key":"B37","doi-asserted-by":"publisher","first-page":"e00712","DOI":"10.1128\/mBio.00712-15","article-title":"Internalization of Pseudomonas aeruginosa Strain PAO1 into epithelial cells is promoted by interaction of a T6SS effector with the microtubule network","volume":"6","author":"Sana","year":"2015","journal-title":"mBio"},{"key":"B38","doi-asserted-by":"publisher","first-page":"27095","DOI":"10.1074\/jbc.M112.376368","article-title":"The second type VI secretion system of Pseudomonas aeruginosa strain PAO1 is regulated by quorum sensing and Fur and modulates internalization in epithelial cells","volume":"287","author":"Sana","year":"2012","journal-title":"J. Biol. Chem."},{"key":"B39","doi-asserted-by":"publisher","first-page":"e76030","DOI":"10.1371\/journal.pone.0076030","article-title":"Divergent control of two type VI secretion systems by RpoN in Pseudomonas aeruginosa","volume":"8","author":"Sana","year":"2013","journal-title":"PLoS ONE"},{"key":"B40","doi-asserted-by":"publisher","first-page":"4213","DOI":"10.1128\/AAC.00507-08","article-title":"Novel genetic determinants of low-level aminoglycoside resistance in Pseudomonas aeruginosa","volume":"52","author":"Schurek","year":"2008","journal-title":"Antimicrob. Agents Chemother."},{"key":"B41","doi-asserted-by":"publisher","first-page":"47","DOI":"10.1016\/0003-2697(87)90612-9","article-title":"Universal chemical assay for the detection and determination of siderophores","volume":"160","author":"Schwyn","year":"1987","journal-title":"Anal. Biochem."},{"key":"B42","doi-asserted-by":"publisher","first-page":"197","DOI":"10.1046\/j.1365-2958.1998.01054.x","article-title":"Intracellular multiplication and human macrophage killing by Legionella pneumophila are inhibited by conjugal components of IncQ plasmid RSF1010","volume":"30","author":"Segal","year":"1998","journal-title":"Mol. Microbiol."},{"key":"B43","doi-asserted-by":"crossref","first-page":"2117","DOI":"10.1128\/IAI.67.5.2117-2124.1999","article-title":"Legionella pneumophila utilizes the same genes to multiply within Acanthamoeba castellanii and human macrophages","volume":"67","author":"Segal","year":"1999","journal-title":"Infect. Immun."},{"key":"B44","doi-asserted-by":"publisher","first-page":"5983","DOI":"10.1128\/IAI.72.10.5983-5992.2004","article-title":"Legionella pneumophila DotU and IcmF are required for stability of the Dot\/Icm complex","volume":"72","author":"Sexton","year":"2004","journal-title":"Infect. Immun."},{"key":"B45","doi-asserted-by":"publisher","first-page":"453","DOI":"10.1146\/annurev-micro-121809-151619","article-title":"Structure and regulation of the type VI secretion system","volume":"66","author":"Silverman","year":"2012","journal-title":"Annu. Rev. Microbiol."},{"key":"B46","doi-asserted-by":"publisher","first-page":"2725","DOI":"10.1093\/molbev\/mst197","article-title":"MEGA6: molecular evolutionary genetics Analysis version 6.0","volume":"30","author":"Tamura","year":"2013","journal-title":"Mol. Biol. Evol."},{"key":"B47","doi-asserted-by":"publisher","first-page":"715","DOI":"10.1073\/pnas.96.2.715","article-title":"Killing of Caenorhabditis elegans by Pseudomonas aeruginosa used to model mammalian bacterial pathogenesis","volume":"96","author":"Tan","year":"1999","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"B48","doi-asserted-by":"publisher","first-page":"4673","DOI":"10.1093\/nar\/22.22.4673","article-title":"CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice","volume":"22","author":"Thompson","year":"1994","journal-title":"Nucleic Acids Res."},{"key":"B49","doi-asserted-by":"publisher","first-page":"e1005020","DOI":"10.1371\/journal.ppat.1005020","article-title":"Type VI secretion system transports Zn2+ to combat multiple stresses and host immunity","volume":"11","author":"Wang","year":"2015","journal-title":"PLoS Pathog."},{"key":"B50","doi-asserted-by":"publisher","first-page":"847","DOI":"10.1093\/bioinformatics\/17.9.847","article-title":"InterProScan\u2013an integration platform for the signature-recognition methods in InterPro","volume":"17","author":"Zdobnov","year":"2001","journal-title":"Bioinformatics"},{"key":"B51","doi-asserted-by":"publisher","first-page":"557","DOI":"10.1111\/1462-2920.12005","article-title":"A type VI secretion system regulated by OmpR in Yersinia pseudotuberculosis functions to maintain intracellular pH homeostasis","volume":"15","author":"Zhang","year":"2013","journal-title":"Environ. Microbiol."},{"key":"B52","doi-asserted-by":"publisher","first-page":"1192","DOI":"10.1111\/j.1365-2958.2007.05993.x","article-title":"Dissection of a type VI secretion system in Edwardsiella tarda","volume":"66","author":"Zheng","year":"2007","journal-title":"Mol. Microbiol."}],"container-title":["Frontiers in Cellular and Infection Microbiology"],"original-title":[],"deposited":{"date-parts":[[2020,9,9]],"date-time":"2020-09-09T02:22:37Z","timestamp":1599618157000},"score":1,"resource":{"primary":{"URL":"http:\/\/journal.frontiersin.org\/article\/10.3389\/fcimb.2015.00070"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,10,1]]},"references-count":52,"URL":"https:\/\/doi.org\/10.3389\/fcimb.2015.00070","relation":{},"ISSN":["2235-2988"],"issn-type":[{"value":"2235-2988","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,10,1]]}}}