{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T22:40:23Z","timestamp":1790030423822,"version":"4.0.1"},"reference-count":53,"publisher":"Frontiers Media SA","content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Cell. Infect. Microbiol."],"DOI":"10.3389\/fcimb.2017.00211","type":"journal-article","created":{"date-parts":[[2017,5,24]],"date-time":"2017-05-24T07:15:41Z","timestamp":1495610141000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":21,"title":["Treatment with pCramoll Alone and in Combination with Fluconazole Provides Therapeutic Benefits in C. gattii Infected Mice"],"prefix":"10.3389","volume":"7","author":[{"given":"Jannyson J.","family":"Jand\u00fa","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marliete C.","family":"Costa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julliana R. A.","family":"Santos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fernanda M.","family":"Andrade","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thais F.","family":"Magalh\u00e3es","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M\u00e1rcia V.","family":"Silva","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria C. A. B.","family":"Castro","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luanna C. B. B.","family":"Coelho","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aline G.","family":"Gomes","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tatiane A.","family":"Paix\u00e3o","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel A.","family":"Santos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria T. S.","family":"Correia","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2017,5,24]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"e2788","DOI":"10.1371\/journal.pntd.0002788","article-title":"Recombinant paracoccin reproduces the biological properties of the native protein and induces protective Th1 immunity against Paracoccidioides brasiliensis infection","volume":"8","author":"Alegre-Maller","year":"2014","journal-title":"PLoS. Negl. Trop. Dis."},{"key":"B2","doi-asserted-by":"publisher","first-page":"352","DOI":"10.1093\/mmy\/myt011","article-title":"IFN-\u03b3 impairs Trichophyton rubrum proliferation in a murine model of dermatophytosis through the production of IL-1\u03b2 and reactive oxygen species","volume":"78","author":"Baltazar","year":"2014","journal-title":"Med. Mycol."},{"key":"B3","doi-asserted-by":"publisher","first-page":"554","DOI":"10.1525\/abt.2011.73.9.9","article-title":"A quick & automated method for measuring cell area using imageJ","volume":"73","author":"Baviskar","year":"2011","journal-title":"Am. Biol. Teach."},{"key":"B4","doi-asserted-by":"publisher","first-page":"3800","DOI":"10.1128\/IAI.00184-15","article-title":"Genetically modified live attenuated Leishmania donovani parasites induce innate immunity through classical activation of macrophages that direct the Th1 response in mice","volume":"83","author":"Bhattacharya","year":"2015","journal-title":"Infect. Immun."},{"key":"B5","doi-asserted-by":"publisher","first-page":"4548","DOI":"10.4049\/jimmunol.1400648","article-title":"Inflammasome activation by Campylobacter jejuni","volume":"193","author":"Bouwman","year":"2014","journal-title":"J. Immunol."},{"key":"B6","doi-asserted-by":"publisher","first-page":"120","DOI":"10.1128\/IAI.01065-08","article-title":"Evidence the role of monocytes in dissemination and brain invasion by Cryptococcus neoformans","volume":"77","author":"Charlier","year":"2009","journal-title":"Infect. Immun."},{"key":"B7","doi-asserted-by":"publisher","first-page":"789","DOI":"10.1093\/cid\/cis529","article-title":"Clinical manifestations of Cryptococcus gattii infection: determinants of neurological sequelae and death","volume":"5","author":"Chen","year":"2012","journal-title":"Clin. Infect. Dis."},{"key":"B8","doi-asserted-by":"publisher","first-page":"499","DOI":"10.1086\/313992","article-title":"Epidemiology and host- and variety dependent characteristics of infection due to Cryptococcus neoformans in Australia and New Zealand","volume":"31","author":"Chen","year":"2000","journal-title":"Clin. Infect. Dis."},{"key":"B9","doi-asserted-by":"publisher","first-page":"261","DOI":"10.1007\/BF02786865","article-title":"Purification of a glucose\/manose specific Lectin. Isoforma 1, from seeds of Cratylia mollis Mart. (Camaratu bean)","volume":"55","author":"Correia","year":"1995","journal-title":"Appl. Biochem. Biotechol."},{"key":"B10","doi-asserted-by":"publisher","first-page":"187","DOI":"10.1016\/j.ijmm.2016.03.010","article-title":"The absence of microbiota delays the inflammatory response to Cryptococcus gattii","volume":"306","author":"Costa","year":"2016","journal-title":"Int. J. Med. Microbiol."},{"key":"B11","doi-asserted-by":"publisher","first-page":"848","DOI":"10.1016\/j.ijbiomac.2014.09.045","article-title":"Immunomodulatory effects of pCramoll and rCramoll on peritoneal exudate cells (PECs) infected and non-infected with Staphylococcus aureus","volume":"72","author":"da Silva","year":"2015","journal-title":"Int. J. Biol. Macromol."},{"key":"B12","doi-asserted-by":"publisher","first-page":"20","DOI":"10.3389\/fmicb.2014.00020","article-title":"Plant lectins and toll-like receptors: implications in therapy for microbial infections","volume":"5","author":"da Silva","year":"2014","journal-title":"Front. Microbiol."},{"key":"B13","doi-asserted-by":"publisher","first-page":"44","DOI":"10.2174\/157340721001140725000701","article-title":"Cratylia mollis lectin: a versatile tool for biomedical studies","volume":"10","author":"da Silva","year":"2014","journal-title":"Curr. Bioact. Comp."},{"key":"B14","doi-asserted-by":"publisher","first-page":"164","DOI":"10.3389\/fcimb.2016.00164","article-title":"Lectins from Synadenium carinatum (ScLL) and Artocarpus heterophyllus (ArtinM) are able to induce beneficial immunomodulatory effects in a murine model for treatment of Toxoplasma gondii infection","volume":"6","author":"de Souza","year":"2016","journal-title":"Front. Cell. Infect. Microbiol"},{"key":"B15","doi-asserted-by":"publisher","first-page":"519","DOI":"10.3390\/molecules20010519","article-title":"Insights into animal and plant lectins with antimicrobial activities","volume":"20","author":"Dias","year":"2015","journal-title":"Molecules"},{"key":"B16","doi-asserted-by":"publisher","first-page":"1801","DOI":"10.1093\/jac\/dkt114","article-title":"The role of oxidative and nitrosative bursts caused by azoles and amphotericin B against the fungal pathogen Cryptococcus gattii","volume":"68","author":"Ferreira","year":"2013","journal-title":"J. Antimicrob. Chemother."},{"key":"B17","doi-asserted-by":"publisher","first-page":"4600","DOI":"10.1128\/AAC.00466-15","article-title":"Heteroresistance to itraconazole alters the morphology and increases the virulence of Cryptococcus gattii","volume":"59","author":"Ferreira","year":"2015","journal-title":"Antimicrob. Agents Chemother."},{"key":"B18","doi-asserted-by":"crossref","first-page":"501","DOI":"10.1128\/CMR.12.4.501","article-title":"Antifungal agents: mode of action, mechanisms of resistance, and correlation of these mechanisms with bacterial resistance","volume":"12","author":"Ghannoum","year":"1999","journal-title":"Clin. Microbiol. Rev."},{"key":"B19","doi-asserted-by":"publisher","first-page":"76","DOI":"10.1016\/j.fgb.2014.11.006","article-title":"Immunity to Cryptococcus neoformans and C. gattii during cryptococcosis. fungal genetics and biology","volume":"78","author":"Gibson","year":"2015"},{"key":"B20","doi-asserted-by":"publisher","first-page":"1879","DOI":"10.1128\/IAI.00222-16","article-title":"DAP12 inhibits pulmonary immune responses to Cryptococcus neoformans","volume":"84","author":"Heung","year":"2016","journal-title":"Infect. Immun."},{"key":"B21","doi-asserted-by":"publisher","first-page":"467","DOI":"10.1016\/j.immuni.2004.08.018","article-title":"Interleukin-17 family members and inflammation","volume":"21","author":"Kolls","year":"2004","journal-title":"Immunity"},{"key":"B22","doi-asserted-by":"publisher","first-page":"193","DOI":"10.1038\/nrmicro2522","article-title":"Expanding fungal pathogenesis: cryptococcus breaks out of the opportunistic box","volume":"9","author":"Kronstad","year":"2011","journal-title":"Nat. Rev. Microbiol."},{"key":"B23","doi-asserted-by":"publisher","first-page":"177","DOI":"10.1111\/j.1365-2990.2006.00706.x","article-title":"Behavioural and histopathological alterations in mice with cerebral malaria","volume":"32","author":"Lackner","year":"2006","journal-title":"Neuropathol. Appl. Neurobiol."},{"key":"B24","doi-asserted-by":"publisher","first-page":"105","DOI":"10.3389\/fmicb.2016.00105","article-title":"Cryptococcus and phagocytes: complex interactions that influence disease outcome","volume":"7","author":"Leopold","year":"2016","journal-title":"Front. Microbiol."},{"key":"B25","doi-asserted-by":"publisher","first-page":"378","DOI":"10.1016\/j.intimp.2011.12.010","article-title":"Artin M enhances TNF-\u03b1 production and phagocytosis of Candida albicans mediated by dectin-1 and mannose receptors","volume":"12","author":"Loyola","year":"2012","journal-title":"Int. Immunopharmacol."},{"key":"B26","doi-asserted-by":"publisher","first-page":"12980","DOI":"10.1073\/pnas.0902963106","article-title":"The fatal fungal outbreak on Vancouver Island is characterized by enhanced intracellular parasitism driven by mitochondrial regulation","volume":"106","author":"Ma","year":"2009","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"B27","doi-asserted-by":"publisher","first-page":"57","DOI":"10.1016\/j.biologicals.2003.12.001","article-title":"Mitogenic activity of Cratylia mollis lectin on human lymphocytes","volume":"32","author":"Maciel","year":"2004","journal-title":"Biologicals"},{"key":"B28","doi-asserted-by":"publisher","first-page":"105","DOI":"10.1038\/nprot.2007.8","article-title":"A method to enable the investigation of murine bronchial immune cells, their cytokines and mediators","volume":"2","author":"Maxeiner","year":"2007","journal-title":"Nat. Protoc."},{"key":"B29","doi-asserted-by":"publisher","first-page":"1631","DOI":"10.1002\/ptr.3156","article-title":"Immunomodulatory response of Cramoll 1,4 lectin on experimental lymphocytes","volume":"24","author":"Melo","year":"2010","journal-title":"Phytother. Res."},{"key":"B30","doi-asserted-by":"publisher","first-page":"113","DOI":"10.1016\/j.ijpharm.2011.02.011","article-title":"Healing activity induce by Cramoll1,4 lectin in healthy an immunocompromised mice","volume":"408","author":"Melo","year":"2011","journal-title":"Intr. J. Pharm."},{"key":"B31","doi-asserted-by":"publisher","first-page":"1525","DOI":"10.1007\/s10096-010-1034-8","article-title":"Correlation of the in vitro antifungal drug susceptibility with the in vivo activity of fluconazole in a murine model of cerebral infection caused by Cryptococcus gattii","volume":"29","author":"Mendes","year":"2010","journal-title":"Eur J. Clin. Microbiol. Infect. Dis."},{"key":"B32","doi-asserted-by":"publisher","first-page":"937","DOI":"10.1128\/IAI.01477-13","article-title":"Interleukin-17A enhances host defense against cryptococcal lung infection through effects mediated by leukocyte recruitment, activation, and gamma interferon production","volume":"82","author":"Murdock","year":"","journal-title":"Infect. Immun."},{"key":"B33","doi-asserted-by":"publisher","first-page":"4107","DOI":"10.4049\/jimmunol.1400650","article-title":"Early or late IL-10 blockade enhances Th1 and Th17 effector responses and promotes fungal clearance in mice with cryptococcal lung infection","volume":"193","author":"Murdock","year":"","journal-title":"J. Immunol."},{"key":"B34","doi-asserted-by":"publisher","first-page":"263968","DOI":"10.1155\/2013\/263968","article-title":"Cratylia mollis1,4 lectin: a new biotechnological tool in IL-6, IL-17A, IL-22, and IL-23 induction and generation of immunological memory","volume":"2013","author":"Oliveira","year":"2013","journal-title":"Biomed. Res. Int."},{"key":"B35","doi-asserted-by":"publisher","first-page":"1269","DOI":"10.2217\/fmb.10.93","article-title":"Mechanisms of cryptococcal virulence and persistence","volume":"5","author":"Olszewski","year":"2014","journal-title":"Future Microbiol."},{"key":"B36","doi-asserted-by":"publisher","first-page":"945397","DOI":"10.1155\/2011\/945397","article-title":"Radiolabeling of cramoll 1,4: evaluation of the biodistribution. Rev. Int. J","volume":"2011","author":"Patricio","year":"2011","journal-title":"Pept."},{"key":"B37","doi-asserted-by":"publisher","first-page":"39","DOI":"10.4303\/jnp\/N100402","article-title":"Development of a murine model of neuroparacoccidioidomycosis","volume":"1","author":"Pedroso","year":"2010","journal-title":"J. Neuroparasitology."},{"key":"B38","doi-asserted-by":"publisher","first-page":"291","DOI":"10.1086\/649858","article-title":"Clinical practice guidelines for the management of cryptococcal disease: 2010 update by the infectious diseases society of America","volume":"50","author":"Perfect","year":"2010","journal-title":"Clin. Infect. Dis."},{"key":"B39","doi-asserted-by":"publisher","first-page":"585","DOI":"10.1111\/myc.12510","article-title":"Evaluation of antifungal combination against Cryptococcus spp","volume":"59","author":"Reichert-Lima","year":"2016","journal-title":"Mycoses"},{"key":"B40","doi-asserted-by":"publisher","first-page":"711","DOI":"10.1007\/s003359900551","article-title":"Behavioral and functional analysis of mouse phenotype: SHIRPA, a proposed protocol for comprehensive phenotype assessment","volume":"8","author":"Rogers","year":"1997","journal-title":"Mamm. Genome"},{"key":"B41","doi-asserted-by":"publisher","first-page":"183","DOI":"10.1016\/j.tim.2006.02.003","article-title":"Protective tolerance to fungi: the role of IL-10 and tryptophan catabolism","volume":"14","author":"Romani","year":"2006","journal-title":"Trends Microbiol."},{"key":"B42","doi-asserted-by":"publisher","first-page":"2553","DOI":"10.1128\/AAC.06098-11","article-title":"Dynamic interaction between fluconazole and amphotericin B against Cryptococcus gattii","volume":"56","author":"Santos","year":"2012","journal-title":"Antimicrob. Agents Chemother."},{"key":"B43","doi-asserted-by":"publisher","first-page":"e112669","DOI":"10.1371\/journal.pone.0112669","article-title":"Fluconazole alters the polysaccharide capsule of Cryptococcus gattii and leads to distinct behaviors in murine Cryptococcosis","volume":"19","author":"Santos","year":"2014","journal-title":"PLoS ONE"},{"key":"B44","doi-asserted-by":"publisher","first-page":"789","DOI":"10.1093\/cid\/cis529","article-title":"Clinical manifestations of Cryptococcus gattii infection: determinants of neurological sequelae and death","volume":"55","author":"Sharon","year":"2012","journal-title":"Clin. Infect. Dis."},{"key":"B45","doi-asserted-by":"publisher","first-page":"139","DOI":"10.1038\/nm1551","article-title":"A brief history of T(H)17, the first major revision in the T(H)1\/T(H)2 hypothesis of T cell-mediated tissue damage","volume":"13","author":"Steinman","year":"2007","journal-title":"Nat. Med."},{"key":"B46","doi-asserted-by":"publisher","first-page":"1432","DOI":"10.1093\/jac\/dks060","article-title":"A murine model of Cryptococcus gattii meningoencephalitis","volume":"67","author":"Thompson","year":"2012","journal-title":"J. Antimicrob. Chemother."},{"key":"B47","doi-asserted-by":"publisher","first-page":"3450","DOI":"10.1128\/IAI.00297-09","article-title":"Cytokine signaling regulates the outcome of intracellular macrophage parasitism by Cryptococcus neoformans","volume":"77","author":"Voelz","year":"2009","journal-title":"Infect. Immun."},{"key":"B48","doi-asserted-by":"publisher","first-page":"prot5080","DOI":"10.1101\/pdb.prot5080","article-title":"Bone Marrow-Derived Macrophages (BMM): isolation and applications","volume":"8","author":"Weischenfeldt","year":"2008","journal-title":"CSH Protoc"},{"key":"B49","doi-asserted-by":"publisher","first-page":"1427","DOI":"10.1016\/S1286-4579(02)00024-2","article-title":"Metabolites released by Cryptococcus neoformans var. neoformans and var. gattii differentially affect human neutrophil function","volume":"4","author":"Wright","year":"2002","journal-title":"Microbes Infect."},{"key":"B50","doi-asserted-by":"publisher","first-page":"645","DOI":"10.1002\/eji.200839102","article-title":"IL-17\/Th17 in anti-fungal immunity: what's new?","volume":"39","author":"Zelante","year":"2009","journal-title":"Eur. J. Immunol."},{"key":"B51","doi-asserted-by":"publisher","first-page":"2626","DOI":"10.1128\/IAI.00259-13","article-title":"Fungal and parasitic infections: congenic strains of the filamentous form of Cryptococcus neoformans for studies of fungal morphogenesis and virulence","volume":"81","author":"Zhai","year":"2013","journal-title":"Infect. Immun."},{"key":"B52","doi-asserted-by":"publisher","DOI":"10.1002\/0471142735.im1401s83","article-title":"The isolation and characterization of murine macrophages","author":"Zhang","year":"2008","journal-title":"Curr. Protoc. Immunol."},{"key":"B53","doi-asserted-by":"publisher","first-page":"5237","DOI":"10.4049\/jimmunol.1203017","article-title":"IL-17 stimulates differentiation of human anti-inflammatory macrophages and phagocytosis of apoptotic neutrophils in response to IL-10 and glucocorticoids","volume":"190","author":"Zizzo","year":"2013","journal-title":"J. Immunol."}],"container-title":["Frontiers in Cellular and Infection Microbiology"],"original-title":[],"link":[{"URL":"http:\/\/journal.frontiersin.org\/article\/10.3389\/fcimb.2017.00211\/full","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,9,25]],"date-time":"2019-09-25T00:34:47Z","timestamp":1569371687000},"score":1,"resource":{"primary":{"URL":"http:\/\/journal.frontiersin.org\/article\/10.3389\/fcimb.2017.00211\/full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,5,24]]},"references-count":53,"alternative-id":["10.3389\/fcimb.2017.00211"],"URL":"https:\/\/doi.org\/10.3389\/fcimb.2017.00211","relation":{},"ISSN":["2235-2988"],"issn-type":[{"value":"2235-2988","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,5,24]]},"article-number":"211"}}