{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T00:58:25Z","timestamp":1790038705244,"version":"4.0.1"},"reference-count":115,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2018,3,22]],"date-time":"2018-03-22T00:00:00Z","timestamp":1521676800000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100007637","name":"Departamento Administrativo de Ciencia, Tecnolog\u00eda e Innovaci\u00f3n","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100007637","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100006071","name":"Facultad de Ciencias, Universidad de los Andes","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100006071","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Cell. Infect. Microbiol."],"DOI":"10.3389\/fcimb.2018.00083","type":"journal-article","created":{"date-parts":[[2018,3,22]],"date-time":"2018-03-22T04:12:23Z","timestamp":1521691943000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":17,"title":["A CTP Synthase Undergoing Stage-Specific Spatial Expression Is Essential for the Survival of the Intracellular Parasite Toxoplasma gondii"],"prefix":"10.3389","volume":"8","author":[{"given":"Heidy Y.","family":"Narvaez-Ortiz","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrea J.","family":"Lopez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nishith","family":"Gupta","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Barbara H.","family":"Zimmermann","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2018,3,22]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"125","DOI":"10.1038\/nmeth.2301","article-title":"Conditional genome engineering in Toxoplasma gondii uncovers alternative invasion mechanisms","volume":"10","author":"Andenmatten","year":"2013","journal-title":"Nat. Methods"},{"key":"B2","doi-asserted-by":"publisher","first-page":"1045","DOI":"10.1242\/bio.201410165","article-title":"Nucleotide synthesis is regulated by cytoophidium formation during neurodevelopment and adaptive metabolism","volume":"3","author":"Aughey","year":"2014","journal-title":"Biol. Open"},{"key":"B3","doi-asserted-by":"publisher","first-page":"e03638","DOI":"10.7554\/eLife.03638","article-title":"Large-scale filament formation inhibits the activity of CTP synthetase","volume":"3","author":"Barry","year":"2014","journal-title":"Elife"},{"key":"B4","first-page":"602","article-title":"Disruptions in nucleotide metabolism can cause pathological conditions","volume-title":"Biochemistry","author":"Berg","year":"2002"},{"key":"B5","doi-asserted-by":"publisher","first-page":"607","DOI":"10.1128\/MMBR.64.3.607-623.2000","article-title":"Lytic cycle of Toxoplasma gondii","volume":"64","author":"Black","year":"2000","journal-title":"Microbiol. Mol. Biol. Rev."},{"key":"B6","doi-asserted-by":"publisher","first-page":"463","DOI":"10.1146\/annurev-micro-091014-104100","article-title":"Lytic cycle of Toxoplasma gondii: 15 years later","volume":"69","author":"Blader","year":"2015","journal-title":"Annu. Rev. Microbiol."},{"key":"B7","doi-asserted-by":"publisher","first-page":"12998","DOI":"10.1073\/pnas.0903831106","article-title":"Host-derived glucose and its transporter in the obligate intracellular pathogen Toxoplasma gondii are dispensable by glutaminolysis","volume":"106","author":"Blume","year":"2009","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"B8","doi-asserted-by":"publisher","first-page":"2963","DOI":"10.1007\/s00018-014-1567-6","article-title":"Glutamine deprivation initiates reversible assembly of mammalian rods and rings","volume":"71","author":"Calise","year":"2014","journal-title":"Cell. Mol. Life Sci."},{"key":"B9","doi-asserted-by":"publisher","first-page":"35","DOI":"10.1016\/B978-0-12-800097-7.00002-6","article-title":"Molecular cell biology and immunobiology of mammalian rod\/ring structures","volume":"308","author":"Carcamo","year":"2014","journal-title":"Int. Rev. Cell Mol. Biol."},{"key":"B10","doi-asserted-by":"publisher","first-page":"e29690","DOI":"10.1371\/journal.pone.0029690","article-title":"Induction of cytoplasmic rods and rings structures by inhibition of the CTP and GTP synthetic pathway in mammalian cells","volume":"6","author":"Carcamo","year":"2011","journal-title":"PLoS ONE"},{"key":"B11","doi-asserted-by":"publisher","first-page":"690492","DOI":"10.1155\/2015\/690492","article-title":"Reviving Lonidamine and 6-Diazo-5-oxo-L-norleucine to be used in combination for metabolic cancer therapy","volume":"2015","author":"Cervantes-Madrid","year":"2015","journal-title":"Biomed Res. Int."},{"key":"B12","doi-asserted-by":"publisher","first-page":"3550","DOI":"10.1242\/jcs.175265","article-title":"Cytoophidium assembly reflects upregulation of IMPDH activity","volume":"128","author":"Chang","year":"2015","journal-title":"J. Cell Sci."},{"key":"B13","doi-asserted-by":"publisher","first-page":"333","DOI":"10.1016\/j.plipres.2008.03.004","article-title":"CTP synthetase and its role in phospholipid synthesis in the yeast Saccharomyces cerevisiae","volume":"47","author":"Chang","year":"2008","journal-title":"Prog. Lipid Res."},{"key":"B14","doi-asserted-by":"publisher","first-page":"17613","DOI":"10.1074\/jbc.M702799200","article-title":"Phosphorylation of human CTP synthetase 1 by protein kinase C: identification of Ser(462) and Thr(455) as major sites of phosphorylation","volume":"282","author":"Chang","year":"2007","journal-title":"J. Biol. Chem."},{"key":"B15","doi-asserted-by":"publisher","first-page":"391","DOI":"10.1016\/j.jgg.2011.08.004","article-title":"Glutamine analogs promote cytoophidium assembly in human and Drosophila cells","volume":"38","author":"Chen","year":"2011","journal-title":"J. Genet. Genomics"},{"key":"B16","doi-asserted-by":"publisher","first-page":"5367","DOI":"10.1074\/jbc.M610993200","article-title":"Phosphorylation of human CTP synthetase 1 by protein kinase A: identification of Thr455 as a major site of phosphorylation","volume":"282","author":"Choi","year":"2007","journal-title":"J. Biol. Chem."},{"key":"B17","doi-asserted-by":"publisher","first-page":"869","DOI":"10.1002\/cbic.200800697","article-title":"Rapid matrix-assisted refolding of histidine-tagged proteins","volume":"10","author":"Dashivets","year":"2009","journal-title":"Chembiochem"},{"key":"B18","doi-asserted-by":"publisher","first-page":"611","DOI":"10.1002\/med.20153","article-title":"Another ten stories in antiviral drug discovery (part C): \u201cOld\u201d and \u201cnew\u201d antivirals, strategies, and perspectives","volume":"29","author":"De Clercq","year":"2009","journal-title":"Med. Res. Rev."},{"key":"B19","doi-asserted-by":"publisher","first-page":"888","DOI":"10.1016\/j.bbadis.2009.01.013","article-title":"Hypoglycosylation due to dolichol metabolism defects","volume":"1792","author":"Denecke","year":"2009","journal-title":"Biochim. Biophys. Acta Mol. Basis Dis."},{"key":"B20","doi-asserted-by":"publisher","first-page":"3075","DOI":"10.1021\/ja01594a036","article-title":"6-Diazo-5-oxo-L-norleucine, a new tumor-inhibitory substance. II. isolation and characterization","volume":"78","author":"Dion","year":"1956","journal-title":"J. Am. Chem. Soc."},{"key":"B21","doi-asserted-by":"publisher","first-page":"14010","DOI":"10.1074\/jbc.271.24.14010","article-title":"Insertional tagging, cloning, and expression of the Toxoplasma gondii hypoxanthine-xanthine-guanine phosphoribosyltransferase gene. Use as a selectable marker for stable transformation","volume":"271","author":"Donald","year":"1996","journal-title":"J. Biol. Chem."},{"key":"B22","doi-asserted-by":"publisher","first-page":"5749","DOI":"10.1073\/pnas.92.12.5749","article-title":"Insertional mutagenesis and marker rescue in a protozoan parasite: cloning of the uracil phosphoribosyltransferase locus from Toxoplasma gondii","volume":"92","author":"Donald","year":"1995","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"B23","doi-asserted-by":"crossref","first-page":"267","DOI":"10.1128\/CMR.11.2.267","article-title":"Structures of Toxoplasma gondii tachyzoites, bradyzoites, and sporozoites and biology and development of tissue cysts","volume":"11","author":"Dubey","year":"1998","journal-title":"Clin. Microbiol. Rev."},{"key":"B24","doi-asserted-by":"publisher","first-page":"e3899","DOI":"10.1371\/journal.pone.0003899","article-title":"Computational analysis and experimental validation of gene predictions in Toxoplasma gondii","volume":"3","author":"Dybas","year":"2008","journal-title":"PLoS ONE"},{"key":"B25","doi-asserted-by":"publisher","first-page":"129","DOI":"10.1016\/j.molbiopara.2010.04.004","article-title":"The Hsp90 co-chaperone p23 of Toxoplasma gondii: identification, functional analysis and dynamic interactome determination","volume":"172","author":"Echeverria","year":"2010","journal-title":"Mol. Biochem. Parasitol."},{"key":"B26","doi-asserted-by":"publisher","first-page":"13491","DOI":"10.1021\/bi051282o","article-title":"Mechanisms of product feedback regulation and drug resistance in cytidine triphosphate synthetases from the structure of a CTP-inhibited complex","volume":"44","author":"Endrizzi","year":"2005","journal-title":"Biochemistry"},{"key":"B27","doi-asserted-by":"publisher","first-page":"11858","DOI":"10.1074\/jbc.M611580200","article-title":"Expression, purification, characterization, and in vivo targeting of trypanosome CTP synthetase for treatment of African sleeping sickness","volume":"282","author":"Fijolek","year":"2007","journal-title":"J. Biol. Chem."},{"key":"B28","doi-asserted-by":"publisher","first-page":"926","DOI":"10.1038\/415926a","article-title":"De novo pyrimidine biosynthesis is required for virulence of Toxoplasma gondii","volume":"415","author":"Fox","year":"2002","journal-title":"Nature"},{"key":"B29","doi-asserted-by":"publisher","first-page":"89","DOI":"10.1016\/S0020-7519(02)00214-X","article-title":"Organisation and sequence determination of glutamine-dependent carbamoyl phosphate synthetase II in Toxoplasma gondii","volume":"33","author":"Fox","year":"2003","journal-title":"Int. J. Parasitol."},{"key":"B30","doi-asserted-by":"publisher","first-page":"3744","DOI":"10.1128\/IAI.00287-10","article-title":"Avirulent uracil auxotrophs based on disruption of orotidine-5\u2032-monophosphate decarboxylase elicit protective immunity to Toxoplasma gondii","volume":"78","author":"Fox","year":"2010","journal-title":"Infect. Immun."},{"key":"B31","doi-asserted-by":"publisher","first-page":"1193","DOI":"10.1128\/EC.00297-10","article-title":"Type II Toxoplasma gondii KU80 knockout strains enable functional analysis of genes required for cyst development and latent infection","volume":"10","author":"Fox","year":"2011","journal-title":"Eukaryot. Cell"},{"key":"B32","doi-asserted-by":"publisher","first-page":"93","DOI":"10.1016\/S0166-6851(98)00154-6","article-title":"Stable transformation of Toxoplasma gondii based on a pyrimethamine resistant trifunctional dihydrofolate reductase-cytosine deaminase-thymidylate synthase gene that confers sensitivity to 5-fluorocytosine","volume":"98","author":"Fox","year":"1999","journal-title":"Mol. Biochem. Parasitol."},{"key":"B33","doi-asserted-by":"publisher","first-page":"e29998","DOI":"10.1371\/journal.pone.0029998","article-title":"Transcriptomic analysis of toxoplasma development reveals many novel functions and structures specific to sporozoites and oocysts","volume":"7","author":"Fritz","year":"2012","journal-title":"PLoS ONE"},{"key":"B34","doi-asserted-by":"publisher","first-page":"195","DOI":"10.1016\/j.jgg.2015.04.004","article-title":"Pyrimidine metabolism: dynamic and versatile pathways in pathogens and cellular development","volume":"42","author":"Garavito","year":"2015","journal-title":"J. Genet. Genomics"},{"key":"B35","doi-asserted-by":"publisher","first-page":"87","DOI":"10.1016\/S0076-6879(02)50957-5","article-title":"Transformation of yeast by lithium acetate\/single-stranded carrier DNA\/polyethylene glycol method","volume":"350","author":"Gietz","year":"2002","journal-title":"Meth. Enzymol."},{"key":"B36","doi-asserted-by":"publisher","first-page":"1303","DOI":"10.1093\/ije\/30.6.1303","article-title":"Effect of prenatal treatment on mother to child transmission of Toxoplasma gondii: retrospective cohort study of 554 mother-child pairs in Lyon, France","volume":"30","author":"Gilbert","year":"2001","journal-title":"Int. J. Epidemiol."},{"key":"B37","doi-asserted-by":"publisher","first-page":"242","DOI":"10.1016\/j.yexcr.2014.01.029","article-title":"CTP synthase forms cytoophidia in the cytoplasm and nucleus","volume":"323","author":"Gou","year":"2014","journal-title":"Exp. Cell Res."},{"key":"B38","doi-asserted-by":"publisher","first-page":"16345","DOI":"10.1074\/jbc.M501523200","article-title":"Selective disruption of phosphatidylcholine metabolism of the intracellular parasite Toxoplasma gondii arrests its growth","volume":"280","author":"Gupta","year":"2005","journal-title":"J. Biol. Chem."},{"key":"B39","doi-asserted-by":"publisher","first-page":"38328","DOI":"10.1074\/jbc.M509622200","article-title":"Expression of human CTP synthetase in Saccharomyces cerevisiae reveals phosphorylation by protein kinase A","volume":"280","author":"Han","year":"2005","journal-title":"J. Biol. Chem."},{"key":"B40","doi-asserted-by":"publisher","first-page":"217","DOI":"10.1016\/S0002-9440(10)63645-0","article-title":"Identification of novel cellular targets in biliary tract cancers using global gene expression technology","volume":"163","author":"Hansel","year":"2003","journal-title":"Am. J. Pathol."},{"key":"B41","doi-asserted-by":"publisher","first-page":"6809","DOI":"10.1074\/jbc.M113.509406","article-title":"Phosphatidylethanolamine synthesis in the parasite mitochondrion is required for efficient growth but dispensable for survival of Toxoplasma gondii","volume":"289","author":"Hartmann","year":"2014","journal-title":"J. Biol. Chem."},{"key":"B42","doi-asserted-by":"publisher","first-page":"539","DOI":"10.1016\/S0006-2952(99)00035-0","article-title":"Role of antimetabolites of purine and pyrimidine nucleotide metabolism in tumor cell differentiation","volume":"58","author":"Hatse","year":"1999","journal-title":"Biochem. Pharmacol."},{"key":"B43","doi-asserted-by":"publisher","first-page":"907","DOI":"10.1016\/0002-9343(87)90151-3","article-title":"Assessment of therapy for toxoplasma encephalitis","volume":"82","author":"Haverkos","year":"1987","journal-title":"Am. J. Med."},{"key":"B44","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1016\/S0167-4781(98)00108-0","article-title":"Molecular cloning and characterization of the Plasmodium falciparum cytidine triphosphate synthetase gene","volume":"1399","author":"Hendriks","year":"1998","journal-title":"Biochim. Biophys. Acta Gene Struct. Expr."},{"key":"B45","doi-asserted-by":"publisher","first-page":"6412","DOI":"10.1073\/pnas.111139498","article-title":"Trypanosoma brucei CTP synthetase: a target for the treatment of African sleeping sickness","volume":"98","author":"Hofer","year":"2001","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"B46","doi-asserted-by":"publisher","first-page":"530","DOI":"10.1128\/EC.00358-08","article-title":"Tagging of endogenous genes in a Toxoplasma gondii strain lacking Ku80","volume":"8","author":"Huynh","year":"2009","journal-title":"Eukaryot. Cell"},{"key":"B47","doi-asserted-by":"publisher","first-page":"31","DOI":"10.2174\/138945007779315524","article-title":"Targeting purine and pyrimidine metabolism in human apicomplexan parasites","volume":"8","author":"Hyde","year":"2007","journal-title":"Curr. Drug Targets"},{"key":"B48","doi-asserted-by":"publisher","first-page":"24","DOI":"10.1111\/j.1550-7408.1993.tb04877.x","article-title":"Pyrimidine salvage pathways in Toxoplasma gondii","volume":"40","author":"Iltzsch","year":"1993","journal-title":"J. Eukaryot. Microbiol."},{"key":"B49","doi-asserted-by":"publisher","first-page":"739","DOI":"10.1038\/ncb2087","article-title":"The metabolic enzyme CTP synthase forms cytoskeletal filaments","volume":"12","author":"Ingerson-Mahar","year":"2010","journal-title":"Nat. Cell Biol."},{"key":"B50","doi-asserted-by":"publisher","first-page":"2627","DOI":"10.1093\/nar\/21.11.2627","article-title":"A PCR-based method for high stringency screening of DNA libraries","volume":"21","author":"Israel","year":"1993","journal-title":"Nucleic Acids Res."},{"key":"B51","doi-asserted-by":"crossref","first-page":"577","DOI":"10.1016\/B978-0-12-396481-6.00017-9","article-title":"Genetic Manipulation of Toxoplasma gondii","volume-title":"Toxoplasma gondii, 2nd Edn.","author":"Jacot","year":"2014"},{"key":"B52","doi-asserted-by":"publisher","first-page":"431","DOI":"10.1111\/j.1550-7408.1963.tb01702.x","article-title":"In vivo activity of L-azaserine against Trypanosoma equiperdum","volume":"10","author":"Jaffe","year":"1963","journal-title":"J. Protozool."},{"key":"B53","doi-asserted-by":"publisher","first-page":"206","DOI":"10.1074\/jbc.M507056200","article-title":"Regulation of the interaction of inosine monophosphate dehydrogenase with mycophenolic acid by GTP","volume":"281","author":"Ji","year":"2006","journal-title":"J. Biol. Chem."},{"key":"B54","doi-asserted-by":"publisher","first-page":"6562","DOI":"10.1128\/JB.185.22.6562-6574.2003","article-title":"CTP limitation increases expression of CTP synthase in Lactococcus lactis","volume":"185","author":"J\u00f8rgensen","year":"2003","journal-title":"J. Bacteriol."},{"key":"B55","doi-asserted-by":"publisher","first-page":"33727","DOI":"10.1074\/jbc.M110.178566","article-title":"Regulation of human cytidine triphosphate synthetase 2 by phosphorylation","volume":"285","author":"Kassel","year":"2010","journal-title":"J. Biol. Chem."},{"key":"B56","doi-asserted-by":"publisher","first-page":"911","DOI":"10.1126\/science.8235614","article-title":"Gene replacement in Toxoplasma gondii with chloramphenicol acetyltransferase as selectable marker","volume":"262","author":"Kim","year":"1993","journal-title":"Science"},{"key":"B57","first-page":"258","article-title":"The rediscovery of DON (6-Diazo-5-oxo-L-norleucine)","volume-title":"Cancer Chemo- and Immunopharmacology SE - 30 Recent Results in Cancer Research","author":"Kisner","year":"1980"},{"key":"B58","doi-asserted-by":"publisher","first-page":"7145","DOI":"10.1074\/jbc.M116.765487","article-title":"Two phylogenetically and compartmentally distinct CDP-diacylglycerol synthases cooperate for lipid biogenesis in Toxoplasma gondii","volume":"292","author":"Kong","year":"2017","journal-title":"J. Biol. Chem."},{"key":"B59","doi-asserted-by":"publisher","first-page":"94","DOI":"10.1016\/0002-9343(88)90014-9","article-title":"Treatment of central nervous system toxoplasmosis with pyrimethamine\/sulfadiazine combination in 35 patients with the acquired immunodeficiency syndrome","volume":"84","author":"Leport","year":"1988","journal-title":"Am. J. Med."},{"key":"B60","doi-asserted-by":"publisher","first-page":"1121","DOI":"10.1073\/pnas.62.4.1121","article-title":"Negative cooperativity in regulatory enzymes","volume":"62","author":"Levitzki","year":"1969","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"B61","doi-asserted-by":"publisher","first-page":"241","DOI":"10.1021\/bi00752a015","article-title":"Role of an allosteric effector. Guanosine triphosphate activation in cytosine triphosphate synthetase","volume":"11","author":"Levitzki","year":"1972","journal-title":"Biochemistry"},{"key":"B62","doi-asserted-by":"crossref","first-page":"765","DOI":"10.1016\/S0021-9258(20)89934-7","article-title":"Enzymatic amination of uridine triphosphate to cytidine triphosphate","volume":"222","author":"Lieberman","year":"1956","journal-title":"J. Biol. Chem."},{"key":"B63","doi-asserted-by":"crossref","first-page":"249","DOI":"10.1016\/S0166-6851(96)02635-7","article-title":"Isolation, characterization and expression of the gene encoding cytidine triphosphate synthetase from Giardia intestinalis","volume":"78","author":"Lim","year":"1996","journal-title":"Mol. Biochem. Parasitol."},{"key":"B64","doi-asserted-by":"publisher","first-page":"17738","DOI":"10.1074\/jbc.M400143200","article-title":"CMP-N-acetylneuraminic acid synthetase from Escherichia coli K1 is a bifunctional enzyme: identification of minimal catalytic domain for synthetase activity and novel functional domain for platelet-activating factor acetylhydrolase activity","volume":"279","author":"Liu","year":"2004","journal-title":"J. Biol. Chem."},{"key":"B65","doi-asserted-by":"publisher","first-page":"281","DOI":"10.1016\/S1673-8527(09)60046-1","article-title":"Intracellular compartmentation of CTP synthase in Drosophila","volume":"37","author":"Liu","year":"2010","journal-title":"J. Genet. Genom."},{"key":"B66","doi-asserted-by":"publisher","first-page":"349","DOI":"10.1146\/annurev-cellbio-111315-124907","article-title":"The cytoophidium and its kind: filamentation and compartmentation of metabolic enzymes","volume":"32","author":"Liu","year":"2016","journal-title":"Annu. Rev. Cell Dev. Biol."},{"key":"B67","doi-asserted-by":"crossref","first-page":"80","DOI":"10.1016\/S0021-9258(18)63424-6","article-title":"The subunit structure and subunit interactions of cytidine triphosphate synthetase","volume":"245","author":"Long","year":"1970","journal-title":"J. Biol. Chem."},{"key":"B68","doi-asserted-by":"crossref","first-page":"4715","DOI":"10.1016\/S0021-9258(18)99515-3","article-title":"Cytidine triphosphate synthetase of Escherichia coli B. I. Purification and kinetics","volume":"242","author":"Long","year":"1967","journal-title":"J. Biol. Chem."},{"key":"B69","doi-asserted-by":"publisher","first-page":"4204","DOI":"10.1111\/j.1432-1033.2004.04360.x","article-title":"Alternative substrates for wild-type and L109A E. coli CTP synthases","volume":"271","author":"Lunn","year":"2004","journal-title":"Eur. J. Biochem."},{"key":"B70","doi-asserted-by":"publisher","first-page":"507","DOI":"10.1038\/nsmb.3407","article-title":"Human CTP synthase filament structure reveals the active enzyme conformation","volume":"24","author":"Lynch","year":"2017","journal-title":"Nat. Struct. Mol. Biol."},{"key":"B71","doi-asserted-by":"publisher","first-page":"213","DOI":"10.1016\/j.bbapap.2004.03.002","article-title":"Inhibition of E. coli CTP synthase by the \u201cpositive\u201d allosteric effector GTP","volume":"1699","author":"MacDonnell","year":"2004","journal-title":"Biochim. Biophys. Acta Prot. Proteom."},{"key":"B72","doi-asserted-by":"publisher","first-page":"199","DOI":"10.1016\/j.bbapap.2005.11.021","article-title":"The role of lysine residues 297 and 306 in nucleoside triphosphate regulation of E. coli CTP synthase: inactivation by 2\u2032, 3\u2032-dialdehyde ATP and mutational analyses","volume":"1764","author":"MacLeod","year":"2006","journal-title":"Biochim. Biophys. Acta"},{"key":"B73","doi-asserted-by":"publisher","first-page":"205","DOI":"10.1007\/s000180050145","article-title":"The mechanism of glutamine-dependent amidotransferases","volume":"54","author":"Massi\u00e8re","year":"1998","journal-title":"Cell. Mol. Life Sci."},{"key":"B74","doi-asserted-by":"publisher","first-page":"115e","DOI":"10.1093\/nar\/29.22.e115","article-title":"Modulation of myosin A expression by a newly established tetracycline repressor-based inducible system in Toxoplasma gondii","volume":"29","author":"Meissner","year":"2001","journal-title":"Nucleic Acids Res."},{"key":"B75","doi-asserted-by":"publisher","first-page":"837","DOI":"10.1126\/science.1074553","article-title":"Role of Toxoplasma gondii myosin A in powering parasite gliding and host cell invasion","volume":"298","author":"Meissner","year":"2002","journal-title":"Science"},{"key":"B76","doi-asserted-by":"publisher","first-page":"5513","DOI":"10.1128\/JB.183.19.5513-5522.2001","article-title":"Regulation of transcription of the Bacillus subtilis pyrG gene, encoding cytidine triphosphate synthetase","volume":"183","author":"Meng","year":"2001","journal-title":"J. Bacteriol."},{"key":"B77","doi-asserted-by":"publisher","first-page":"1965","DOI":"10.1016\/S0140-6736(04)16412-X","article-title":"Toxoplasmosis","volume":"363","author":"Montoya","year":"2004","journal-title":"Lancet"},{"key":"B78","doi-asserted-by":"publisher","first-page":"917","DOI":"10.1016\/j.chembiol.2015.05.016","article-title":"Thiophenecarboxamide derivatives activated by EthA kill Mycobacterium tuberculosis by inhibiting the CTP synthetase pyrG","volume":"22","author":"Mori","year":"2015","journal-title":"Chem. Biol."},{"key":"B79","doi-asserted-by":"publisher","first-page":"1482","DOI":"10.1021\/bi00355a003","article-title":"Continuous association of Escherichia coli single-stranded DNA binding protein with stable complexes of recA protein and single-stranded DNA","volume":"25","author":"Morrical","year":"1986","journal-title":"Biochemistry"},{"key":"B80","doi-asserted-by":"publisher","first-page":"24982","DOI":"10.1074\/jbc.270.42.24982","article-title":"Differential biochemical regulation of the URA7- and URA8-encoded CTP synthetases from Saccharomyces cerevisiae","volume":"270","author":"Nadkarni","year":"1995","journal-title":"J. Biol. Chem."},{"key":"B81","doi-asserted-by":"publisher","first-page":"15225","DOI":"10.1074\/jbc.M117.802702","article-title":"A plant\/fungal-type phosphoenolpyruvate carboxykinase located in the parasite mitochondrion ensures glucose-independent survival of Toxoplasma gondii","volume":"292","author":"Nitzsche","year":"2017","journal-title":"J. Biol. Chem."},{"key":"B82","doi-asserted-by":"publisher","first-page":"126","DOI":"10.1074\/jbc.M114.624619","article-title":"Metabolic cooperation of glucose and glutamine is essential for the lytic cycle of obligate intracellular parasite Toxoplasma gondii","volume":"291","author":"Nitzsche","year":"2016","journal-title":"J. Biol. Chem."},{"key":"B83","doi-asserted-by":"publisher","first-page":"2282","DOI":"10.1091\/mbc.E14-04-0912","article-title":"Common regulatory control of CTP synthase enzyme activity and filament formation","volume":"25","author":"Noree","year":"2014","journal-title":"Mol. Biol. Cell"},{"key":"B84","doi-asserted-by":"publisher","first-page":"541","DOI":"10.1083\/jcb.201003001","article-title":"Identification of novel filament-forming proteins in Saccharomyces cerevisiae and Drosophila melanogaster","volume":"190","author":"Noree","year":"2010","journal-title":"J. Cell Biol."},{"key":"B85","doi-asserted-by":"crossref","first-page":"18992","DOI":"10.1074\/jbc.273.30.18992","article-title":"Effect of CTP synthetase regulation by CTP on phospholipid synthesis in Saccharomyces cerevisiae","volume":"273","author":"Ostrander","year":"1998","journal-title":"J. Biol. Chem."},{"key":"B86","doi-asserted-by":"publisher","first-page":"431","DOI":"10.1007\/BF00281793","article-title":"Use of synthetic lethal mutants to clone and characterize a novel CTP synthetase gene in Saccharomyces cerevisiae","volume":"242","author":"Ozier-Kalogeropoulos","year":"1994","journal-title":"Mol. Gen. Genet."},{"key":"B87","doi-asserted-by":"publisher","first-page":"229","DOI":"10.1042\/BJ20110641","article-title":"Overexpression of a cytosolic pyrophosphatase (TgPPase) reveals a regulatory role of PP i in glycolysis for Toxoplasma gondii","volume":"440","author":"Pace","year":"2011","journal-title":"Biochem. J."},{"key":"B88","doi-asserted-by":"publisher","first-page":"20785","DOI":"10.1074\/jbc.M301394200","article-title":"Phosphorylation of CTP synthetase on Ser36, Ser330, Ser354, and Ser454 regulates the levels of CTP and phosphatidylcholine synthesis in Saccharomyces cerevisiae","volume":"278","author":"Park","year":"2003","journal-title":"J. Biol. Chem."},{"key":"B89","doi-asserted-by":"publisher","first-page":"26","DOI":"10.1016\/0014-4894(78)90077-2","article-title":"Toxoplasma gondii: the enzymic defect of a mutant resistant to 5-fluorodeoxyuridine","volume":"44","author":"Pfefferkorn","year":"1978","journal-title":"Exp. Parasitol."},{"key":"B90","doi-asserted-by":"publisher","first-page":"93","DOI":"10.1016\/j.molbiopara.2006.03.003","article-title":"Cloning and preliminary characterization of the dihydroorotase from Toxoplasma gondii","volume":"148","author":"Robles Lopez","year":"2006","journal-title":"Mol. Biochem. Parasitol."},{"key":"B91","volume-title":"Molecular Cloning: A Laboratory Manual","author":"Sambrook","year":"2001"},{"key":"B92","doi-asserted-by":"publisher","first-page":"16289","DOI":"10.1074\/jbc.M112.347138","article-title":"Conditional mutagenesis of a novel choline kinase demonstrates plasticity of phosphatidylcholine biogenesis and gene expression in Toxoplasma gondii","volume":"287","author":"Sampels","year":"2012","journal-title":"J. Biol. Chem."},{"key":"B93","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1046\/j.1365-313X.1994.6010067.x","article-title":"SUC1 and SUC2: two sucrose transporters from Arabidopsis thaliana; expression and characterization in baker's yeast and identification of the histidine-tagged protein","volume":"6","author":"Sauer","year":"1994","journal-title":"Plant J."},{"key":"B94","volume-title":"Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems","author":"Segel","year":"1993"},{"key":"B95","first-page":"139","article-title":"CMP-Sialic acid synthetase: the point of constriction in the sialylation pathway","volume-title":"SialoGlyco Chemistry and Biology I. Topics in Current Chemistry","author":"Sellmeier","year":"2013"},{"key":"B96","doi-asserted-by":"publisher","first-page":"e1002392","DOI":"10.1371\/journal.ppat.1002392","article-title":"A systematic screen to discover and analyze apicoplast proteins identifies a conserved and essential protein import factor","volume":"7","author":"Sheiner","year":"2011","journal-title":"PLoS Pathog."},{"key":"B97","doi-asserted-by":"publisher","first-page":"1423","DOI":"10.1016\/j.cell.2016.08.019","article-title":"A genome-wide CRISPR screen in Toxoplasma identifies essential apicomplexan genes","volume":"166","author":"Sidik","year":"2016","journal-title":"Cell"},{"key":"B98","doi-asserted-by":"publisher","first-page":"5188","DOI":"10.1016\/j.bmcl.2011.07.054","article-title":"Activation and inhibition of CTP synthase from Trypanosoma brucei, the causative agent of African sleeping sickness","volume":"21","author":"Steeves","year":"2011","journal-title":"Bioorg. Med. Chem. Lett."},{"key":"B99","doi-asserted-by":"publisher","first-page":"8","DOI":"10.1006\/prep.2001.1603","article-title":"A new vector for high-throughput, ligation-independent cloning encoding a tobacco etch virus protease cleavage site","volume":"25","author":"Stols","year":"2002","journal-title":"Protein Expr. Purif."},{"key":"B100","doi-asserted-by":"publisher","first-page":"1184","DOI":"10.15252\/embr.201438688","article-title":"Ack kinase regulates CTP synthase filaments during Drosophila oogenesis","volume":"15","author":"Strochlic","year":"2014","journal-title":"EMBO Rep."},{"key":"B101","doi-asserted-by":"publisher","first-page":"e51096","DOI":"10.1371\/journal.pone.0051096","article-title":"Different characteristics and nucleotide binding properties of inosine monophosphate dehydrogenase (IMPDH) isoforms","volume":"7","author":"Thomas","year":"2012","journal-title":"PLoS ONE"},{"key":"B102","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/S1046-5928(02)00641-1","article-title":"Practical considerations in refolding proteins from inclusion bodies","volume":"28","author":"Tsumoto","year":"2003","journal-title":"Prot. Expr. Purif."},{"key":"B103","first-page":"1375","article-title":"The roles of uridine-cytidine kinase and CTP synthetase in the synthesis of CTP in malignant human T-lymphocytic cells","volume":"8","author":"van den Berg","year":"1994","journal-title":"Leukemia"},{"key":"B104","doi-asserted-by":"publisher","first-page":"108","DOI":"10.1016\/0959-8049(94)00442-8","article-title":"Cytidine triphosphate (CTP) synthetase activity during cell cycle progression in normal and malignant T-lymphocytic cells","author":"van den Berg","year":"1995","journal-title":"Eur. J. Cancer"},{"key":"B105","doi-asserted-by":"publisher","first-page":"257","DOI":"10.1007\/0-306-46843-3_50","article-title":"Isoforms of human CTP synthetase","volume":"486","author":"van Kuilenburg","year":"2000","journal-title":"Adv. Exp. Med. Biol."},{"key":"B106","doi-asserted-by":"publisher","first-page":"667","DOI":"10.1007\/978-1-4615-5381-6_129","article-title":"Increased activity of cytidine triphosphate synthetase in pediatric acute lymphoblastic leukemia","volume":"431","author":"Verschuur","year":"1998","journal-title":"Adv. Exp. Med. Biol."},{"key":"B107","doi-asserted-by":"publisher","first-page":"38002","DOI":"10.1074\/jbc.M100531200","article-title":"Cloning and verification of the Lactococcus lactis pyrG gene and characterization of the gene product, CTP synthase","volume":"276","author":"Wadskov-Hansen","year":"2001","journal-title":"J. Biol. Chem."},{"key":"B108","doi-asserted-by":"publisher","first-page":"17","DOI":"10.1016\/S0003-9861(03)00085-7","article-title":"Substrate inhibition of Lactococcus lactis cytidine 5\u2032-triphosphate synthase by ammonium chloride is enhanced by salt-dependent tetramer dissociation","volume":"413","author":"Willemoes","year":"2003","journal-title":"Arch. Biochem. Biophys."},{"key":"B109","doi-asserted-by":"publisher","first-page":"856","DOI":"10.1111\/j.1742-4658.2004.04525.x","article-title":"Lid L11 of the glutamine amidotransferase domain of CTP synthase mediates allosteric GTP activation of glutaminase activity","volume":"272","author":"Willemo\u00ebs","year":"2005","journal-title":"FEBS J."},{"key":"B110","doi-asserted-by":"publisher","first-page":"71","DOI":"10.1038\/271071a0","article-title":"Increased CTP synthetase activity in cancer cells","volume":"271","author":"Williams","year":"1978","journal-title":"Nature"},{"key":"B111","doi-asserted-by":"publisher","first-page":"631","DOI":"10.1046\/j.1365-2958.1996.d01-1717.x","article-title":"Chlamydia trachomatis CTP synthetase: molecular characterization and developmental regulation of expression","volume":"22","author":"Wylie","year":"","journal-title":"Mol. Microbiol."},{"key":"B112","doi-asserted-by":"publisher","first-page":"15393","DOI":"10.1074\/jbc.271.26.15393","article-title":"A single point mutation in CTP synthetase of Chlamydia trachomatis confers resistance to cyclopentenyl cytosine","volume":"271","author":"Wylie","year":"","journal-title":"J. Biol. Chem."},{"key":"B113","doi-asserted-by":"publisher","first-page":"10785","DOI":"10.1021\/bi00201a028","article-title":"Purification and characterization of CTP synthetase, the product of the URA7 gene in Saccharomyces cerevisiae","volume":"33","author":"Yang","year":"1994","journal-title":"Biochemistry"},{"key":"B114","doi-asserted-by":"publisher","first-page":"200","DOI":"10.1016\/j.molbiopara.2005.06.004","article-title":"Functional expression of the gene encoding cytidine triphosphate synthetase from Plasmodium falciparum which contains two novel sequences that are potential antimalarial targets","volume":"143","author":"Yuan","year":"2005","journal-title":"Mol. Biochem. Parasitol."},{"key":"B115","doi-asserted-by":"publisher","first-page":"1092","DOI":"10.1242\/bio.20149613","article-title":"Asymmetric inheritance of cytoophidia in Schizosaccharomyces pombe","volume":"3","author":"Zhang","year":"2014","journal-title":"Biol. Open"}],"container-title":["Frontiers in Cellular and Infection Microbiology"],"original-title":[],"link":[{"URL":"http:\/\/journal.frontiersin.org\/article\/10.3389\/fcimb.2018.00083\/full","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,8,16]],"date-time":"2022-08-16T17:56:10Z","timestamp":1660672570000},"score":1,"resource":{"primary":{"URL":"http:\/\/journal.frontiersin.org\/article\/10.3389\/fcimb.2018.00083\/full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,3,22]]},"references-count":115,"alternative-id":["10.3389\/fcimb.2018.00083"],"URL":"https:\/\/doi.org\/10.3389\/fcimb.2018.00083","relation":{},"ISSN":["2235-2988"],"issn-type":[{"value":"2235-2988","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,3,22]]},"article-number":"83"}}