{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T14:57:46Z","timestamp":1790261866586,"version":"4.1.0"},"reference-count":48,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2018,4,10]],"date-time":"2018-04-10T00:00:00Z","timestamp":1523318400000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000774","name":"Defense Threat Reduction Agency","doi-asserted-by":"publisher","award":["D-CZ-10-00001"],"award-info":[{"award-number":["D-CZ-10-00001"]}],"id":[{"id":"10.13039\/100000774","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004488","name":"Hrvatska Zaklada za Znanost","doi-asserted-by":"publisher","award":["IP-2016-06-9003"],"award-info":[{"award-number":["IP-2016-06-9003"]}],"id":[{"id":"10.13039\/501100004488","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001823","name":"Ministerstvo \u0160kolstv\u00ed, Ml\u00e1de\u017ee a T\u011blov\u00fdchovy","doi-asserted-by":"publisher","award":["LM2015062"],"award-info":[{"award-number":["LM2015062"]}],"id":[{"id":"10.13039\/501100001823","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Cell. Infect. Microbiol."],"DOI":"10.3389\/fcimb.2018.00111","type":"journal-article","created":{"date-parts":[[2018,4,10]],"date-time":"2018-04-10T12:25:30Z","timestamp":1523363130000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":16,"title":["Francisella tularensis D-Ala D-Ala Carboxypeptidase DacD Is Involved in Intracellular Replication and It Is Necessary for Bacterial Cell Wall Integrity"],"prefix":"10.3389","volume":"8","author":[{"given":"Petra","family":"Spidlova","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pavla","family":"Stojkova","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vera","family":"Dankova","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Iva","family":"Senitkova","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marina","family":"Santic","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dominik","family":"Pinkas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vlada","family":"Philimonenko","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiri","family":"Stulik","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2018,4,10]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"e1000284","DOI":"10.1371\/journal.ppat.1000284","article-title":"Glutathione provides a source of cysteine essential for intracellular multiplication of Francisella tularensis","volume":"5","author":"Alkhuder","year":"2009","journal-title":"PLoS Pathog."},{"key":"B2","doi-asserted-by":"publisher","first-page":"2559","DOI":"10.1111\/j.1462-2920.2010.02229.x","article-title":"Molecular complexity orchestrates modulation of phagosome biogenesis and escape to the cytosol of macrophages by Francisella tularensis","volume":"12","author":"Asare","year":"2010","journal-title":"Environ. Microbiol."},{"key":"B3","doi-asserted-by":"publisher","first-page":"2587","DOI":"10.1111\/j.1462-2920.2010.02230.x","article-title":"Molecular bases of proliferation of Francisella tularensis in arthropod vectors","volume":"12","author":"Asare","year":"2010","journal-title":"Environ. Microbiol."},{"key":"B4","doi-asserted-by":"publisher","first-page":"7106","DOI":"10.1128\/jb.178.24.7106-7111.1996","article-title":"dacD, an Escherichia coli gene encoding a novel penicillin-binding protein (PBP6b) with DD-carboxypeptidase activity","volume":"178","author":"Baquero","year":"1996","journal-title":"J. Bacteriol."},{"key":"B5","doi-asserted-by":"publisher","first-page":"207","DOI":"10.1016\/j.ijantimicag.2008.03.017","article-title":"A bioinformatic approach to understanding antibiotic resistance in intracellular bacteria through whole genome analysis","volume":"32","author":"Biswas","year":"2008","journal-title":"Int. J. Antimicrob. Agents"},{"key":"B6","doi-asserted-by":"publisher","first-page":"3502","DOI":"10.1128\/IAI.00226-08","article-title":"MglA and Igl proteins contribute to the modulation of Francisella tularensis live vaccine strain-containing phagosomes in murine macrophages","volume":"76","author":"B\u00f6nquist","year":"2008","journal-title":"Infect. Immun."},{"key":"B7","doi-asserted-by":"publisher","first-page":"205","DOI":"10.1128\/AAC.01224-13","article-title":"Low-molecular-mass penicillin binding protein 6b (DacD) is required for efficient GOB-18 metallo-\u03b2-lactamase biogenesis in Salmonella enterica and Escherichia coli","volume":"58","author":"Brambilla","year":"2014","journal-title":"Antimicrob. Agents Chemother."},{"key":"B8","doi-asserted-by":"publisher","first-page":"6642","DOI":"10.1128\/IAI.01250-06","article-title":"Identification of MglA-regulated genes reveals novel virulence factors in Francisella tularensis","volume":"74","author":"Brotcke","year":"2006","journal-title":"Infect. Immun."},{"key":"B9","doi-asserted-by":"publisher","first-page":"a010314","DOI":"10.1101\/cshperspect.a010314","article-title":"Mechanisms of Francisella tularensis intracellular pathogenesis","volume":"3","author":"Celli","year":"2013","journal-title":"Cold Spring Harb. Perspect. Med"},{"key":"B10","doi-asserted-by":"crossref","first-page":"232","DOI":"10.1128\/AEM.13.2.232-235.1965","article-title":"Evaluation of live tularemia vaccine prepared in a chemically defined medium","volume":"13","author":"Chamberlain","year":"1965","journal-title":"Appl. Microbiol"},{"key":"B11","doi-asserted-by":"publisher","first-page":"F1000","DOI":"10.12688\/f1000research.11388.1","article-title":"Bacterial lipids: powerful modifiers of the innate immune response","volume":"6","author":"Chandler","year":"2017","journal-title":"F1000Res"},{"key":"B12","doi-asserted-by":"publisher","first-page":"1342","DOI":"10.4161\/auto.20808","article-title":"Cytosolic clearance of replication-deficient mutants reveals Francisella tularensis interactions with the autophagic pathway","volume":"8","author":"Chong","year":"2012","journal-title":"Autophagy"},{"key":"B13","doi-asserted-by":"publisher","first-page":"1757","DOI":"10.1128\/IAI.01485-08","article-title":"Francisella tularensis Phagosomal escape does not require acidification of the phagosome","volume":"77","author":"Clemens","year":"2009","journal-title":"Infect. Immun."},{"key":"B14","doi-asserted-by":"publisher","first-page":"510","DOI":"10.1021\/acs.jproteome.5b00864","article-title":"Inactivation of Francisella tularensis gene encoding putative ABC transporter has a pleiotropic effect upon production of various glycoconjugates","volume":"15","author":"Dankova","year":"2016","journal-title":"J. Proteome Res."},{"key":"B15","doi-asserted-by":"crossref","first-page":"3981","DOI":"10.1128\/JB.181.13.3981-3993.1999","article-title":"Escherichia coli mutants lacking all possible combinations of eight penicillin binding proteins: viability, characteristics, and implications for peptidoglycan synthesis","volume":"181","author":"Denome","year":"1999","journal-title":"J. Bacteriol"},{"key":"B16","doi-asserted-by":"publisher","first-page":"2278","DOI":"10.1074\/mcp.M112.024794","article-title":"Possible links between stress defense and the tricarboxylic acid (TCA) Cycle in Francisella Pathogenesis","volume":"12","author":"Dieppedale","year":"2013","journal-title":"Mol. Cell. Proteomics"},{"key":"B17","doi-asserted-by":"publisher","first-page":"20150031","DOI":"10.1098\/rstb.2015.0031","article-title":"Activities and regulation of peptidoglycan synthases","volume":"370","author":"Egan","year":"2015","journal-title":"Philos. Trans. R. Soc. Lond. B. Biol. Sci"},{"key":"B18","doi-asserted-by":"publisher","first-page":"4934","DOI":"10.1128\/IAI.00475-08","article-title":"RipA, a cytoplasmic membrane protein conserved among Francisella species, is required for intracellular survival","volume":"76","author":"Fuller","year":"2008","journal-title":"Infect. Immun."},{"key":"B19","doi-asserted-by":"publisher","first-page":"309","DOI":"10.1016\/j.tim.2008.04.006","article-title":"Physiological functions of D-alanine carboxypeptidases in Escherichia coli","volume":"16","author":"Ghosh","year":"2008","journal-title":"Trends Microbiol."},{"key":"B20","doi-asserted-by":"crossref","first-page":"18988","DOI":"10.1016\/S0021-9258(17)30613-0","article-title":"Growth pattern of the murein sacculus of Escherichia coli","volume":"265","author":"Glauner","year":"1990","journal-title":"J. Biol. Chem"},{"key":"B21","doi-asserted-by":"publisher","first-page":"256","DOI":"10.1128\/jb.176.1.256-259.1994","article-title":"Artifactual processing of penicillin-binding proteins 7 and 1b by the OmpT protease of Escherichia coli","volume":"176","author":"Henderson","year":"1994","journal-title":"J. Bacteriol."},{"key":"B22","doi-asserted-by":"publisher","first-page":"2074","DOI":"10.1128\/jb.177.8.2074-2079.1995","article-title":"Identification and cloning of the gene encoding penicillin-binding protein 7 of Escherichia coli","volume":"177","author":"Henderson","year":"1995","journal-title":"J. Bacteriol."},{"key":"B23","doi-asserted-by":"publisher","first-page":"6112","DOI":"10.1128\/jb.179.19.6112-6121.1997","article-title":"AmpC and AmpH, proteins related to the class C beta-lactamases, bind penicillin and contribute to the normal morphology of Escherichia coli","volume":"179","author":"Henderson","year":"1997","journal-title":"J. Bacteriol."},{"key":"B24","first-page":"55","article-title":"Chapter 4: Utilization of lipid-linked precursors and the formation of peptidoglycan in the process of cell growth and division: membrane enzymes involved in the final steps of peptidoglycan synthesis and the mechanism of their regulation","volume-title":"New Comprehensive Biochemistry","author":"Matsuhashi","year":"1994"},{"key":"B25","doi-asserted-by":"crossref","first-page":"1310","DOI":"10.1128\/jb.178.5.1310-1319.1996","article-title":"Flagellin A is essential for the virulence of Vibrio anguillarum","volume":"178","author":"Milton","year":"1996","journal-title":"J. Bacteriol"},{"key":"B26","doi-asserted-by":"publisher","first-page":"3055","DOI":"10.1128\/JB.183.10.3055-3064.2001","article-title":"Contributions of PBP 5 and DD-carboxypeptidase penicillin binding proteins to maintenance of cell shape in Escherichia coli","volume":"183","author":"Nelson","year":"2001","journal-title":"J. Bacteriol."},{"key":"B27","doi-asserted-by":"publisher","first-page":"503","DOI":"10.3389\/fcimb.2017.00503","article-title":"The multiple localized glyceraldehyde-3-phosphate dehydrogenase contributes to the attenuation of the Francisella tularensis dsbA deletion mutant","volume":"7","author":"Pavkova","year":"2017","journal-title":"Front. Cell. Infect. Microbiol"},{"key":"B28","doi-asserted-by":"publisher","first-page":"e2487","DOI":"10.1371\/journal.pone.0002487","article-title":"A Francisella tularensis Schu S4 purine auxotroph is highly attenuated in mice but offers limited protection against homologous intranasal challenge","volume":"3","author":"Pechous","year":"2008","journal-title":"PLoS ONE"},{"key":"B29","doi-asserted-by":"publisher","first-page":"e00819","DOI":"10.1128\/mBio.00819-16","article-title":"The redundancy of peptidoglycan carboxypeptidases ensures robust cell shape maintenance in Escherichia coli","volume":"7","author":"Peters","year":"2016","journal-title":"MBio"},{"key":"B30","doi-asserted-by":"publisher","first-page":"882","DOI":"10.1080\/21505594.2016.1168550","article-title":"Components of the type six secretion system are substrates of Francisella tularensis Schu S4 DsbA-like FipB protein","volume":"7","author":"Qin","year":"2016","journal-title":"Virulence"},{"key":"B31","doi-asserted-by":"publisher","first-page":"926","DOI":"10.1111\/mmi.12808","article-title":"Identification of disulfide bond isomerase substrates reveals bacterial virulence factors","volume":"94","author":"Ren","year":"2014","journal-title":"Mol. Microbiol."},{"key":"B32","doi-asserted-by":"publisher","first-page":"21659","DOI":"10.1038\/srep21659","article-title":"Characterization of SCO4439, a D-alanyl-D-alanine carboxypeptidase involved in spore cell wall maturation, resistance, and germination in Streptomyces coelicolor","volume":"6","author":"Rioseras","year":"2016","journal-title":"Sci. Rep."},{"key":"B33","doi-asserted-by":"publisher","first-page":"2619","DOI":"10.1128\/AEM.02905-07","article-title":"Targeted inactivation of Francisella tularensis genes by group II introns","volume":"74","author":"Rodriguez","year":"2008","journal-title":"Appl. Environ. Microbiol."},{"key":"B34","doi-asserted-by":"publisher","first-page":"94","DOI":"10.3389\/fcimb.2015.00094","article-title":"From the outside-in: the Francisella tularensis envelope and virulence","volume":"5","author":"Rowe","year":"2015","journal-title":"Front. Cell. Infect. Microbiol."},{"key":"B35","doi-asserted-by":"publisher","first-page":"2391","DOI":"10.1111\/j.1462-5822.2007.00968.x","article-title":"A Francisella tularensis pathogenicity island protein essential for bacterial proliferation within the host cell cytosol","volume":"9","author":"Santic","year":"2007","journal-title":"Cell. Microbiol."},{"key":"B36","doi-asserted-by":"publisher","first-page":"2702","DOI":"10.1099\/mic.0.046227-0","article-title":"PBP5, PBP6 and DacD play different roles in intrinsic \u03b2-lactam resistance of Escherichia coli","volume":"157","author":"Sarkar","year":"2011","journal-title":"Microbiol. Read. Engl"},{"key":"B37","doi-asserted-by":"publisher","first-page":"32031","DOI":"10.1074\/jbc.274.45.32031","article-title":"Cloning and characterization of PBP 1C, a third member of the multimodular class A penicillin-binding proteins of Escherichia coli","volume":"274","author":"Schiffer","year":"1999","journal-title":"J. Biol. Chem."},{"key":"B38","doi-asserted-by":"publisher","first-page":"676","DOI":"10.1038\/nmeth.2019","article-title":"Fiji: an open-source platform for biological-image analysis","volume":"9","author":"Schindelin","year":"2012","journal-title":"Nat. Methods"},{"key":"B39","doi-asserted-by":"publisher","first-page":"784","DOI":"10.1038\/nbt1183-784","article-title":"A broad host range mobilization system for in vivo genetic engineering: transposon mutagenesis in gram negative bacteria","volume":"1","author":"Simon","year":"1983","journal-title":"Nat. Biotechnol."},{"key":"B40","first-page":"230","article-title":"ilastik: interactive learning and segmentation toolkit","volume-title":"Proceedings of Eighth IEEE International Symposium on Biomedical Imaging (ISBI)","author":"Sommer","year":"2011"},{"key":"B41","doi-asserted-by":"publisher","first-page":"249","DOI":"10.1111\/j.1574-695X.1996.tb00246.x","article-title":"Francisella tularensis resistance to bactericidal action of normal human serum","volume":"13","author":"Sorokin","year":"1996","journal-title":"FEMS Immunol. Med. Microbiol."},{"key":"B42","doi-asserted-by":"publisher","first-page":"37","DOI":"10.1556\/030.63.2016.013","article-title":"Identification of two substrates of FTS_1067 protein - An essential virulence factor of Francisella tularensis","volume":"64","author":"Spidlova","year":"2017","journal-title":"Acta Microbiol. Immunol. Hung."},{"key":"B43","doi-asserted-by":"publisher","first-page":"2999","DOI":"10.1073\/pnas.72.8.2999","article-title":"Distinct penicillin binding proteins involved in the division, elongation, and shape of Escherichia coli K12","volume":"72","author":"Spratt","year":"1975","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"B44","doi-asserted-by":"crossref","first-page":"489","DOI":"10.1128\/JB.64.4.489-499.1952","article-title":"Genetic studies on microbial cross resistance to toxic agents. I. Cross resistance of Escherichia coli to fifteen antibiotics","volume":"64","author":"Szybalski","year":"1952","journal-title":"J. Bacteriol."},{"key":"B45","doi-asserted-by":"publisher","first-page":"149","DOI":"10.1111\/j.1574-6976.2007.00094.x","article-title":"Peptidoglycan structure and architecture","volume":"32","author":"Vollmer","year":"","journal-title":"FEMS Microbiol. Rev"},{"key":"B46","doi-asserted-by":"publisher","first-page":"259","DOI":"10.1111\/j.1574-6976.2007.00099.x","article-title":"Bacterial peptidoglycan (murein) hydrolases","volume":"32","author":"Vollmer","year":"","journal-title":"FEMS Microbiol. Rev"},{"key":"B47","doi-asserted-by":"publisher","first-page":"1106","DOI":"10.1186\/s12864-015-2351-1","article-title":"Using host-pathogen protein interactions to identify and characterize Francisella tularensis virulence factors","volume":"16","author":"Wallqvist","year":"2015","journal-title":"BMC Genomics"},{"key":"B48","doi-asserted-by":"publisher","first-page":"1128","DOI":"10.1111\/j.1462-5822.2009.01316.x","article-title":"Intracellular biology and virulence determinants of Francisella tularensis revealed by transcriptional profiling inside macrophages","volume":"11","author":"Wehrly","year":"2009","journal-title":"Cell. Microbiol."}],"container-title":["Frontiers in Cellular and Infection Microbiology"],"original-title":[],"link":[{"URL":"http:\/\/journal.frontiersin.org\/article\/10.3389\/fcimb.2018.00111\/full","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,8,18]],"date-time":"2022-08-18T20:45:51Z","timestamp":1660855551000},"score":1,"resource":{"primary":{"URL":"http:\/\/journal.frontiersin.org\/article\/10.3389\/fcimb.2018.00111\/full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,4,10]]},"references-count":48,"alternative-id":["10.3389\/fcimb.2018.00111"],"URL":"https:\/\/doi.org\/10.3389\/fcimb.2018.00111","relation":{},"ISSN":["2235-2988"],"issn-type":[{"value":"2235-2988","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,4,10]]},"article-number":"111"}}