{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,21]],"date-time":"2026-08-21T11:41:50Z","timestamp":1787312510879,"version":"build-2736575974"},"reference-count":31,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2018,10,19]],"date-time":"2018-10-19T00:00:00Z","timestamp":1539907200000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Cell. Infect. Microbiol."],"DOI":"10.3389\/fcimb.2018.00364","type":"journal-article","created":{"date-parts":[[2018,10,19]],"date-time":"2018-10-19T08:48:05Z","timestamp":1539938885000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":31,"title":["Metagenomic Sequencing From Mosquitoes in China Reveals a Variety of Insect and Human Viruses"],"prefix":"10.3389","volume":"8","author":[{"given":"Pengpeng","family":"Xiao","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chenghui","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jicheng","family":"Han","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaofang","family":"Guo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lv","family":"Xie","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mingyao","family":"Tian","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yiquan","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maopeng","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hao","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingqiang","family":"Ren","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongning","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huijun","family":"Lu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ningyi","family":"Jin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2018,10,19]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"131","DOI":"10.1016\/j.vetmic.2004.11.004","article-title":"Detection of Brazilian bovine respiratory syncytial virus strain by a reverse transcriptase-nested-polymerase chain reaction in experimentally infected calves","volume":"105","author":"Almeida","year":"2005","journal-title":"Vet. Microbiol."},{"key":"B2","doi-asserted-by":"publisher","first-page":"2468","DOI":"10.1038\/srep02468","article-title":"Discovery of a divergent HPIV4 from respiratory secretions using second and third generation metagenomic sequencing","volume":"3","author":"Alquezar-Planas","year":"2013","journal-title":"Sci. Rep"},{"key":"B3","doi-asserted-by":"publisher","first-page":"30","DOI":"10.3390\/v10010030","article-title":"Metagenomic virome analysis of Culex mosquitoes from Kenya and China","volume":"10","author":"Atoni","year":"2018","journal-title":"Viruses"},{"key":"B4","doi-asserted-by":"publisher","first-page":"4911","DOI":"10.3390\/v7092851","article-title":"Insect-specific virus discovery: significance for the arbovirus community","volume":"7","author":"Bolling","year":"2015","journal-title":"Viruses"},{"key":"B5","doi-asserted-by":"publisher","first-page":"e0153881","DOI":"10.1371\/journal.pone.0153881","article-title":"Identification and characterization of two novel RNA viruses from Anopheles gambiae species complex mosquitoes","volume":"11","author":"Carissimo","year":"2016","journal-title":"PLoS ONE"},{"key":"B6","doi-asserted-by":"publisher","first-page":"e0162751","DOI":"10.1371\/journal.pone.0162751","article-title":"Discovery of novel viruses in mosquitoes from the zambezi valley of mozambique","volume":"11","author":"Cholleti","year":"2016","journal-title":"PLoS ONE"},{"key":"B7","doi-asserted-by":"publisher","first-page":"e4324","DOI":"10.7717\/peerj.4324","article-title":"Vector competence of selected North American Anopheles and Culex mosquitoes for Zika virus","volume":"6","author":"Dodson","year":"2018","journal-title":"Peer J"},{"key":"B8","doi-asserted-by":"publisher","first-page":"e3096","DOI":"10.7717\/peerj.3096","article-title":"Vector competence of Anopheles and Culex mosquitoes for Zika virus","volume":"5","author":"Dodson","year":"2017","journal-title":"Peer J"},{"key":"B9","doi-asserted-by":"publisher","first-page":"1903","DOI":"10.1007\/s00705-017-3314-4","article-title":"A novel rhabdovirus, related to Merida virus, in field-collected mosquitoes from Anatolia and Thrace","volume":"162","author":"Ergunay","year":"2017","journal-title":"Arch. Virol"},{"key":"B10","first-page":"51","article-title":"Isolation and identification of the Akabane virus from mosquitoes in Yunnan Province, China","volume":"31","author":"Feng","year":"2015","journal-title":"Bing. Du. Xue. Bao"},{"key":"B11","doi-asserted-by":"publisher","first-page":"48","DOI":"10.1016\/j.jcv.2014.11.014","article-title":"Next generation sequencing improves detection of drug resistance mutations in infants after PMTCT failure","volume":"62","author":"Fisher","year":"2015","journal-title":"J. Clin. Virol"},{"key":"B12","doi-asserted-by":"publisher","first-page":"1","DOI":"10.4137\/EBO.S38518","article-title":"Bioinformatic characterization of mosquito viromes within the Eastern United States and Puerto Rico: discovery of novel viruses","volume":"12","author":"Frey","year":"2016","journal-title":"Evol. Bioinform. Online"},{"key":"B13","doi-asserted-by":"publisher","first-page":"e61950","DOI":"10.1371\/journal.pone.0061950","article-title":"Virome profiling of bats from Myanmar by metagenomic analysis of tissue samples reveals more novel Mammalian viruses","volume":"8","author":"He","year":"2013","journal-title":"PLoS ONE"},{"key":"B14","doi-asserted-by":"publisher","first-page":"939","DOI":"10.1016\/j.jhep.2009.08.004","article-title":"The long and winding road leading to the identification of the hepatitis C virus","volume":"51","author":"Houghton","year":"2009","journal-title":"J. Hepatol"},{"key":"B15","doi-asserted-by":"publisher","first-page":"288","DOI":"10.1016\/j.virol.2016.07.031","article-title":"WestAfrican Anopheles gambiae mosquitoes harbor a taxonomically diverse virome including new insect-specific flaviviruses, mononegaviruses,and totiviruses","volume":"498","author":"Joseph","year":"2016","journal-title":"Virology"},{"key":"B16","doi-asserted-by":"publisher","first-page":"2480","DOI":"10.1128\/JCM.00305-07","article-title":"Dengue virus detection using whole blood for reverse transcriptase PCR and virus isolation","volume":"45","author":"Klungthong","year":"2007","journal-title":"J. Clin. Microbiol"},{"key":"B17","doi-asserted-by":"crossref","first-page":"223","DOI":"10.1016\/1045-1056(91)90039-M","article-title":"A simplified multiwell plate assay for the measurement of hepatitis A virus infectivity","volume":"19","author":"Krah","year":"1991","journal-title":"Biologicals"},{"key":"B18","doi-asserted-by":"publisher","first-page":"374","DOI":"10.3967\/bes2015.052","article-title":"Real-time RT-PCR Assay for the detection of Tahyna Virus","volume":"28","author":"Li","year":"2015","journal-title":"Biomed. Environ. Sci"},{"key":"B19","doi-asserted-by":"publisher","first-page":"e0004452","DOI":"10.1371\/journal.pntd.0004452","article-title":"Small RNA profiling in dengue virus 2-infected aedes mosquito cells reveals viral piRNAs and novel host miRNAs","volume":"10","author":"Miesen","year":"2016","journal-title":"PLoS. Negl. Trop. Dis"},{"key":"B20","doi-asserted-by":"publisher","first-page":"125","DOI":"10.1007\/978-1-4939-1743-3_11","article-title":"Viral detection by high-throughput sequencing","volume":"1236","author":"Motooka","year":"2015","journal-title":"Methods Mol. Biol"},{"key":"B21","doi-asserted-by":"publisher","first-page":"1505","DOI":"10.1099\/0022-1317-75-6-1505","article-title":"Comparison of nucleotide and deduced amino acid sequence of the 5' non-coding region and structural protein genes of the wild-type Japanese encephalitis virus strain SA14 and its attenuated vaccine derivatives","volume":"75","author":"Ni","year":"1994","journal-title":"J. Gen. Virol"},{"key":"B22","doi-asserted-by":"publisher","first-page":"657","DOI":"10.1099\/vir.0.80624-0","article-title":"Mitogen-induced upregulation of hepatitis C virus expression in human lymphoid cells","volume":"86","author":"Pham","year":"2005","journal-title":"J. Gen. Virol"},{"key":"B23","doi-asserted-by":"publisher","first-page":"497","DOI":"10.1089\/vbz.2006.0643","article-title":"Operational trials of remote mosquito trap systems for Japanese encephalitis virus surveillance in the Torres Strait, Australia","volume":"7","author":"Ritchie","year":"2007","journal-title":"Vector. Borne. Zoonotic. Dis"},{"key":"B24","doi-asserted-by":"publisher","first-page":"e0129845","DOI":"10.1371\/journal.pone.0129845","article-title":"A metagenomic survey of viral abundance and diversity in mosquitoes from Hubei province","volume":"10","author":"Shi","year":"2015","journal-title":"PLoS ONE"},{"key":"B25","doi-asserted-by":"publisher","first-page":"121","DOI":"10.1186\/1743-422X-11-121","article-title":"Identification and validation of a novel microRNA-like molecule derived from a cytoplasmic RNA virus antigenome by bioinformatics and experimental approaches","volume":"11","author":"Shi","year":"2014","journal-title":"Virol. J"},{"key":"B26","doi-asserted-by":"publisher","first-page":"e00680","DOI":"10.1128\/JVI.00680-17","article-title":"High-resolution metatranscriptomics reveals the ecological dynamics of mosquito-associated RNA viruses in western Australia","volume":"91","author":"Shi","year":"2017","journal-title":"J. Virol"},{"key":"B27","doi-asserted-by":"publisher","first-page":"e0004052","DOI":"10.1371\/journal.pntd.0004052","article-title":"Tracking dengue virus intra-host genetic diversity during human-to-mosquito transmission","volume":"9","author":"Sim","year":"2015","journal-title":"PLoS Negl. Trop. Dis"},{"key":"B28","doi-asserted-by":"publisher","first-page":"722","DOI":"10.1002\/ana.24499","article-title":"Diagnosing Balamuthia mandrillaris encephalitis with metagenomic deep sequencing","volume":"78","author":"Wilson","year":"2015","journal-title":"Ann. Neurol"},{"key":"B29","doi-asserted-by":"publisher","first-page":"59","DOI":"10.1007\/s12250-018-0015-4","article-title":"Comparative metagenomic profiling of viromes associated with four common mosquito species in China","volume":"33","author":"Xia","year":"2018","journal-title":"Virol. Sin"},{"key":"B30","doi-asserted-by":"publisher","first-page":"4690","DOI":"10.1038\/s41598-018-22945-y","article-title":"Mapping the virome in wild-caught Aedes aegypti from Cairns and Bangkok","volume":"8","author":"Zakrzewski","year":"2018","journal-title":"Sci. Rep"},{"key":"B31","doi-asserted-by":"publisher","first-page":"6795","DOI":"10.1073\/pnas.1304300110","article-title":"Dengue structure differs at the temperatures of its human and mosquito hosts","volume":"110","author":"Zhang","year":"2013","journal-title":"Proc. Natl. Acad. Sci. U.S.A"}],"container-title":["Frontiers in Cellular and Infection Microbiology"],"original-title":[],"link":[{"URL":"https:\/\/www.frontiersin.org\/article\/10.3389\/fcimb.2018.00364\/full","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2018,10,19]],"date-time":"2018-10-19T08:48:08Z","timestamp":1539938888000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.frontiersin.org\/article\/10.3389\/fcimb.2018.00364\/full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,10,19]]},"references-count":31,"alternative-id":["10.3389\/fcimb.2018.00364"],"URL":"https:\/\/doi.org\/10.3389\/fcimb.2018.00364","relation":{},"ISSN":["2235-2988"],"issn-type":[{"value":"2235-2988","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,10,19]]},"article-number":"364"}}