{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T23:10:40Z","timestamp":1790118640648,"version":"4.0.1"},"reference-count":39,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2019,3,22]],"date-time":"2019-03-22T00:00:00Z","timestamp":1553212800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004396","name":"Thailand Research Fund","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004396","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000268","name":"Biotechnology and Biological Sciences Research Council","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000268","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Cell. Infect. Microbiol."],"DOI":"10.3389\/fcimb.2019.00063","type":"journal-article","created":{"date-parts":[[2019,3,22]],"date-time":"2019-03-22T07:52:31Z","timestamp":1553241151000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":20,"title":["Burkholderia pseudomallei BimC Is Required for Actin-Based Motility, Intracellular Survival, and Virulence"],"prefix":"10.3389","volume":"9","author":[{"given":"Varintip","family":"Srinon","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Somjit","family":"Chaiwattanarungruengpaisan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sunee","family":"Korbsrisate","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joanne M.","family":"Stevens","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2019,3,22]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"348","DOI":"10.1016\/j.cell.2015.02.044","article-title":"Virulent Burkholderia species mimic host actin polymerases to drive actin-based motility","volume":"161","author":"Benanti","year":"2015","journal-title":"Cell"},{"key":"B2","doi-asserted-by":"publisher","first-page":"461","DOI":"10.1016\/j.ijmm.2011.03.003","article-title":"Structures and functions of autotransporter proteins in microbial pathogens","volume":"301","author":"Benz","year":"2011","journal-title":"Int. J. Med. Microbiol."},{"key":"B3","doi-asserted-by":"publisher","first-page":"514","DOI":"10.1111\/j.1462-5822.2006.00807.x","article-title":"The bacteria\u2018l gene lfpA influences the potent induction of calcitonin receptor and osteoclast-related genes in Burkholderia pseudomallei-induced TRAP-positive multinucleated giant cells","volume":"9","author":"Boddey","year":"2007","journal-title":"Cell. Microbiol."},{"key":"B4","doi-asserted-by":"publisher","first-page":"e76767","DOI":"10.1371\/journal.pone.0076767","article-title":"Burkholderia mallei and Burkholderia pseudomallei cluster 1 type VI secretion system gene expression is negatively regulated by iron and zinc","volume":"8","author":"Burtnick","year":"2013","journal-title":"PLoS ONE"},{"key":"B5","doi-asserted-by":"publisher","first-page":"1512","DOI":"10.1128\/IAI.01218-10","article-title":"The cluster 1 type VI secretion system is a major virulence determinant in Burkholderia pseudomallei","volume":"79","author":"Burtnick","year":"2011","journal-title":"Infect. Immun."},{"key":"B6","doi-asserted-by":"publisher","first-page":"383","DOI":"10.1128\/CMR.18.2.383-416.2005","article-title":"Melioidosis: epidemiology, pathophysiology, and management","volume":"18","author":"Cheng","year":"2005","journal-title":"Clin. Microbiol. Rev."},{"key":"B7","doi-asserted-by":"publisher","first-page":"411","DOI":"10.4269\/ajtmh.12-0555","article-title":"Clinical definitions of melioidosis","volume":"88","author":"Cheng","year":"2013","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"B8","doi-asserted-by":"publisher","first-page":"30","DOI":"10.1186\/1471-2180-5-30","article-title":"An improved method for rapid generation of unmarked Pseudomonas aeruginosa deletion mutants","volume":"5","author":"Choi","year":"2005","journal-title":"BMC Microbiol."},{"key":"B9","doi-asserted-by":"publisher","first-page":"177","DOI":"10.4269\/ajtmh.2001.65.177","article-title":"A cluster of melioidosis cases from an endemic region is clonal and is linked to the water supply using molecular typing of Burkholderia pseudomallei isolates","volume":"65","author":"Currie","year":"2001","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"B10","doi-asserted-by":"publisher","first-page":"5305","DOI":"10.1128\/AEM.00287-10","article-title":"Association of the melioidosis agent Burkholderia pseudomallei with water parameters in rural water supplies in Northern Australia","volume":"76","author":"Draper","year":"2010","journal-title":"Appl. Environ. Microbiol."},{"key":"B11","doi-asserted-by":"publisher","first-page":"35","DOI":"10.1016\/j.mib.2004.12.013","article-title":"Actin-based motility of intracellular pathogens","volume":"8","author":"Gouin","year":"2005","journal-title":"Curr. Opin. Microbiol."},{"key":"B12","first-page":"35","article-title":"An ultrastructural study of the phagocytosis of Burkholderia pseudomallei","volume":"94","author":"Harley","year":"1998","journal-title":"Microbios"},{"key":"B13","doi-asserted-by":"publisher","first-page":"4675","DOI":"10.1021\/acs.jproteome.6b00760","article-title":"Identification of candidate host cell factors required for actin-based motility of Burkholderia pseudomallei","volume":"15","author":"Jitprasutwit","year":"2016","journal-title":"J. Proteome Res."},{"key":"B14","doi-asserted-by":"crossref","first-page":"782","DOI":"10.1128\/IAI.64.3.782-790.1996","article-title":"Intracellular survival of Burkholderia pseudomallei","volume":"64","author":"Jones","year":"1996","journal-title":"Infect. Immun."},{"key":"B15","doi-asserted-by":"publisher","first-page":"e364","DOI":"10.1371\/journal.pntd.0000364","article-title":"Landscape changes influence the occurrence of the melioidosis bacterium Burkholderia pseudomallei in soil in northern Australia","volume":"3","author":"Kaestli","year":"2009","journal-title":"PLoS Negl. Trop. Dis."},{"key":"B16","doi-asserted-by":"publisher","first-page":"5377","DOI":"10.1128\/IAI.68.9.5377-5384.2000","article-title":"Burkholderia pseudomallei induces cell fusion and actin-associated membrane protrusion: a possible mechanism for cell-to-cell spreading","volume":"68","author":"Kespichayawattana","year":"2000","journal-title":"Infect. Immun."},{"key":"B17","doi-asserted-by":"publisher","first-page":"151","DOI":"10.3389\/fmicb.2011.00151","article-title":"Autotransporters and their role in the virulence of burkholderia pseudomallei and Burkholderia mallei","volume":"2","author":"Lazar Adler","year":"2011","journal-title":"Front. Microbiol."},{"key":"B18","doi-asserted-by":"publisher","first-page":"e0121271","DOI":"10.1371\/journal.pone.0121271","article-title":"Systematic mutagenesis of genes encoding predicted autotransported proteins of Burkholderia pseudomallei identifies factors mediating virulence in mice, net intracellular replication and a novel protein conferring serum resistance","volume":"10","author":"Lazar Adler","year":"2015","journal-title":"PLoS ONE"},{"key":"B19","doi-asserted-by":"publisher","first-page":"15008","DOI":"10.1038\/nmicrobiol.2015.8","article-title":"Predicted global distribution of Burkholderia pseudomallei and burden of melioidosis","volume":"1","author":"Limmathurotsakul","year":"2016","journal-title":"Nat. Microbiol."},{"key":"B20","doi-asserted-by":"publisher","first-page":"1113","DOI":"10.4269\/ajtmh.2010.10-0038","article-title":"Increasing incidence of human melioidosis in Northeast Thailand","volume":"82","author":"Limmathurotsakul","year":"2010","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"B21","doi-asserted-by":"publisher","first-page":"265","DOI":"10.3201\/eid2002.121891","article-title":"Melioidosis caused by Burkholderia pseudomallei in drinking water, Thailand, 2012","volume":"20","author":"Limmathurotsakul","year":"2014","journal-title":"Emerging Infect. Dis."},{"key":"B22","doi-asserted-by":"publisher","first-page":"320","DOI":"10.1016\/j.mimet.2008.12.007","article-title":"Facile construction of unmarked deletion mutants in Burkholderia pseudomallei using sacB counter-selection in sucrose-resistant and sucrose-sensitive isolates","volume":"76","author":"Logue","year":"2009","journal-title":"J. Microbiol. Methods"},{"key":"B23","doi-asserted-by":"publisher","first-page":"408","DOI":"10.1111\/cmi.12376","article-title":"A polar-localized iron-binding protein determines the polar targeting of Burkholderia BimA autotransporter and actin tail formation","volume":"17","author":"Lu","year":"2015","journal-title":"Cell. Microbiol."},{"key":"B24","doi-asserted-by":"publisher","first-page":"351","DOI":"10.1016\/j.chom.2014.08.008","article-title":"An iron-containing dodecameric heptosyltransferase family modifies bacterial autotransporters in pathogenesis","volume":"16","author":"Lu","year":"2014","journal-title":"Cell Host Microbe"},{"key":"B25","doi-asserted-by":"publisher","first-page":"862","DOI":"10.1016\/S0140-6736(64)91581-8","article-title":"Melioidosis: a case-report","volume":"1","author":"Maegraith","year":"1964","journal-title":"Lancet"},{"key":"B26","doi-asserted-by":"publisher","first-page":"623","DOI":"10.1007\/s00203-008-0413-3","article-title":"Inactivation of Burkholderia pseudomallei bsaQ results in decreased invasion efficiency and delayed escape of bacteria from endocytic vesicles","volume":"190","author":"Muangsombut","year":"2008","journal-title":"Arch. Microbiol."},{"key":"B27","doi-asserted-by":"publisher","first-page":"e96298","DOI":"10.1371\/journal.pone.0096298","article-title":"Analysis of the prevalence, secretion and function of a cell cycle-inhibiting factor in the melioidosis pathogen Burkholderia pseudomallei","volume":"9","author":"Pumirat","year":"2014","journal-title":"PLoS ONE"},{"key":"B28","doi-asserted-by":"publisher","first-page":"1445","DOI":"10.1128\/IAI.01368-13","article-title":"VgrG-5 is a Burkholderia type VI secretion system-exported protein required for multinucleated giant cell formation and virulence","volume":"82","author":"Schwarz","year":"2014","journal-title":"Infect. Immun."},{"key":"B29","doi-asserted-by":"publisher","first-page":"8058","DOI":"10.1128\/JB.186.23.8058-8065.2004","article-title":"Novel roles for the AIDA adhesin from diarrheagenic Escherichia coli: cell aggregation and biofilm formation","volume":"186","author":"Sherlock","year":"2004","journal-title":"J. Bacteriol."},{"key":"B30","doi-asserted-by":"publisher","first-page":"1901","DOI":"10.1128\/JB.01455-10","article-title":"Identification of motifs of Burkholderia pseudomallei BimA required for intracellular motility, actin binding, and actin polymerization","volume":"193","author":"Sitthidet","year":"2011","journal-title":"J. Bacteriol."},{"key":"B31","doi-asserted-by":"publisher","first-page":"2418","DOI":"10.1128\/JCM.00368-08","article-title":"Prevalence and sequence diversity of a factor required for actin-based motility in natural populations of Burkholderia species","volume":"46","author":"Sitthidet","year":"2008","journal-title":"J. Clin. Microbiol."},{"key":"B32","doi-asserted-by":"publisher","first-page":"5249","DOI":"10.1128\/JB.00608-10","article-title":"Actin-based motility of Burkholderia thailandensis requires a central acidic domain of BimA that recruits and activates the cellular Arp2\/3 complex","volume":"192","author":"Sitthidet","year":"2010","journal-title":"J. Bacteriol."},{"key":"B33","doi-asserted-by":"publisher","first-page":"7857","DOI":"10.1128\/JB.187.22.7857-7862.2005","article-title":"Actin-binding proteins from Burkholderia mallei and Burkholderia thailandensis can functionally compensate for the actin-based motility defect of a Burkholderia pseudomallei bimA mutant","volume":"187","author":"Stevens","year":"","journal-title":"J. Bacteriol."},{"key":"B34","doi-asserted-by":"publisher","first-page":"40","DOI":"10.1111\/j.1365-2958.2004.04528.x","article-title":"Identification of a bacterial factor required for actin-based motility of Burkholderia pseudomallei","volume":"56","author":"Stevens","year":"","journal-title":"Mol. Microbiol."},{"key":"B35","doi-asserted-by":"publisher","first-page":"649","DOI":"10.1046\/j.1365-2958.2002.03190.x","article-title":"An Inv\/Mxi-Spa-like type III protein secretion system in Burkholderia pseudomallei modulates intracellular behaviour of the pathogen","volume":"46","author":"Stevens","year":"2002","journal-title":"Mol. Microbiol."},{"key":"B36","doi-asserted-by":"publisher","first-page":"1447","DOI":"10.1111\/j.1462-5822.2005.00569.x","article-title":"Caspase-1 dependent macrophage death induced by Burkholderia pseudomallei","volume":"7","author":"Sun","year":"2005","journal-title":"Cell. Microbiol."},{"key":"B37","doi-asserted-by":"publisher","first-page":"1436","DOI":"10.1128\/IAI.01367-13","article-title":"The Type VI secretion system spike protein VgrG5 mediates membrane fusion during intercellular spread by pseudomallei group Burkholderia species","volume":"82","author":"Toesca","year":"2014","journal-title":"Infect. Immun."},{"key":"B38","doi-asserted-by":"publisher","first-page":"11","DOI":"10.1186\/1471-2180-11-11","article-title":"Macrophage and Galleria mellonella infection models reflect the virulence of naturally occurring isolates of B. pseudomallei, B. thailandensis and B. oklahomensis","volume":"11","author":"Wand","year":"2011","journal-title":"BMC Microbiol."},{"key":"B39","doi-asserted-by":"publisher","first-page":"51","DOI":"10.1111\/j.1365-2559.1995.tb00620.x","article-title":"The histopathology of human melioidosis","volume":"26","author":"Wong","year":"1995","journal-title":"Histopathology"}],"container-title":["Frontiers in Cellular and Infection Microbiology"],"original-title":[],"link":[{"URL":"https:\/\/www.frontiersin.org\/article\/10.3389\/fcimb.2019.00063\/full","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,12,2]],"date-time":"2020-12-02T14:36:23Z","timestamp":1606919783000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.frontiersin.org\/article\/10.3389\/fcimb.2019.00063\/full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,3,22]]},"references-count":39,"alternative-id":["10.3389\/fcimb.2019.00063"],"URL":"https:\/\/doi.org\/10.3389\/fcimb.2019.00063","relation":{},"ISSN":["2235-2988"],"issn-type":[{"value":"2235-2988","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,3,22]]},"article-number":"63"}}