{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,15]],"date-time":"2026-06-15T15:20:33Z","timestamp":1781536833302,"version":"3.54.5"},"reference-count":15,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2019,11,12]],"date-time":"2019-11-12T00:00:00Z","timestamp":1573516800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Cell. Infect. Microbiol."],"DOI":"10.3389\/fcimb.2019.00382","type":"journal-article","created":{"date-parts":[[2019,11,12]],"date-time":"2019-11-12T05:07:10Z","timestamp":1573535230000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":27,"title":["Design and Evaluation of a Novel Multiplex Real-Time PCR Melting Curve Assay for the Simultaneous Detection of Nine Sexually Transmitted Disease Pathogens in Genitourinary Secretions"],"prefix":"10.3389","volume":"9","author":[{"given":"Xiao-Mei","family":"Hu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiang-Xia","family":"Xu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li-Xia","family":"Jiang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lian-Rui","family":"Deng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhen-Mei","family":"Gu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiao-Ying","family":"Xie","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui-Cai","family":"Ji","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei-Hua","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li-Ming","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cheng-Nan","family":"Tian","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fang-Li","family":"Song","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shao","family":"Huang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lei","family":"Zheng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tian-Yu","family":"Zhong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2019,11,12]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"569","DOI":"10.1186\/s12879-015-1313-1","article-title":"Prevalence of 7 sexually transmitted organisms by multiplex real-time PCR in Fallopian tube specimens collected from Saudi women with and without ectopic pregnancy","volume":"15","author":"Ashshi","year":"2015","journal-title":"BMC Infect. Dis."},{"key":"B2","doi-asserted-by":"publisher","first-page":"991","DOI":"10.1128\/JCM.03370-14","article-title":"Assessment of coinfection of sexually transmitted pathogen microbes by use of the anyplex II STI-7 molecular kit","volume":"53","author":"Bercot","year":"2015","journal-title":"J. Clin. Microbiol."},{"key":"B3","doi-asserted-by":"publisher","first-page":"471","DOI":"10.1016\/j.eimc.2015.10.014","article-title":"Usefulness of a novel multiplex real-time PCR assay for the diagnosis of sexually-transmitted infections","volume":"34","author":"Fernandez","year":"2016","journal-title":"Enferm. Infecc. Microbiol. Clin."},{"key":"B4","doi-asserted-by":"publisher","first-page":"767","DOI":"10.1016\/j.emc.2018.06.007","article-title":"Management of patients with sexually transmitted infections in the emergency department","volume":"36","author":"Hilbert","year":"2018","journal-title":"Emerg. Med. Clin. North Am."},{"key":"B5","doi-asserted-by":"publisher","first-page":"e19206","DOI":"10.1371\/journal.pone.0019206","article-title":"Multiplex fluorescence melting curve analysis for mutation detection with dual-labeled, self-quenched probes","volume":"6","author":"Huang","year":"2011","journal-title":"PLoS ONE"},{"key":"B6","doi-asserted-by":"publisher","first-page":"20","DOI":"10.1136\/jramc-2015-000488","article-title":"The prevalence of causative organisms of community-acquired urethritis in an age group at high risk for sexually transmitted infections in Korean Soldiers","volume":"163","author":"Kim","year":"2017","journal-title":"J. R. Army Med. Corps"},{"key":"B7","doi-asserted-by":"publisher","first-page":"74","DOI":"10.1016\/j.jiac.2013.08.005","article-title":"Prevalence of sexually transmitted infections among healthy Korean women: implications of multiplex PCR pathogen detection on antibiotic therapy","volume":"20","author":"Kim","year":"2014","journal-title":"J. Infect. Chemother."},{"key":"B8","doi-asserted-by":"publisher","first-page":"1749","DOI":"10.7754\/Clin.Lab.2015.150413","article-title":"Analytical performance of multiplex real-time PCR for six sexually transmitted pathogens","volume":"61","author":"Kim","year":"2015","journal-title":"Clin. Lab."},{"key":"B9","doi-asserted-by":"publisher","first-page":"117","DOI":"10.1016\/j.mimet.2014.12.010","article-title":"Performance evaluation of a DNA chip assay in the identification of major genitourinary pathogens","volume":"109","author":"Kweon","year":"2015","journal-title":"J. Microbiol. Methods"},{"key":"B10","doi-asserted-by":"publisher","first-page":"494","DOI":"10.1007\/s10156-011-0362-7","article-title":"Evaluation of Seeplex(R) STD6 ACE Detection kit for the diagnosis of six bacterial sexually transmitted infections","volume":"18","author":"Lee","year":"2012","journal-title":"J. Infect. Chemother."},{"key":"B11","doi-asserted-by":"publisher","first-page":"47","DOI":"10.1002\/jcla.2058","article-title":"Multiplex polymerase chain reaction: a practical approach","volume":"16","author":"Markoulatos","year":"2002","journal-title":"J. Clin. Lab. Anal."},{"key":"B12","doi-asserted-by":"publisher","first-page":"879","DOI":"10.1111\/apm.12430","article-title":"Evaluation of the new AmpliSens multiplex real-time PCR assay for simultaneous detection of Neisseria gonorrhoeae, Chlamydia trachomatis, Mycoplasma genitalium, and Trichomonas vaginalis","volume":"123","author":"Rumyantseva","year":"2015","journal-title":"APMIS"},{"key":"B13","doi-asserted-by":"publisher","first-page":"163","DOI":"10.1093\/cid\/ciq074","article-title":"Re-evaluating the treatment of nongonococcal urethritis: emphasizing emerging pathogens\u2013a randomized clinical trial","volume":"52","author":"Schwebke","year":"2011","journal-title":"Clin. Infect. Dis."},{"key":"B14","doi-asserted-by":"publisher","first-page":"60","DOI":"10.1136\/sextrans-2018-053597","article-title":"Sexually transmitted infections during pregnancy and subsequent risk of stillbirth and infant mortality in Kenya: a prospective study","volume":"95","author":"Warr","year":"2019","journal-title":"Sex. Transm. Infect."},{"key":"B15","first-page":"1","article-title":"Sexually transmitted diseases treatment guidelines, 2010","volume":"59","author":"Workowski","year":"2010","journal-title":"MMWR Recomm. Rep."}],"container-title":["Frontiers in Cellular and Infection Microbiology"],"original-title":[],"link":[{"URL":"https:\/\/www.frontiersin.org\/article\/10.3389\/fcimb.2019.00382\/full","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,11,12]],"date-time":"2019-11-12T05:07:13Z","timestamp":1573535233000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.frontiersin.org\/article\/10.3389\/fcimb.2019.00382\/full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,11,12]]},"references-count":15,"alternative-id":["10.3389\/fcimb.2019.00382"],"URL":"https:\/\/doi.org\/10.3389\/fcimb.2019.00382","relation":{},"ISSN":["2235-2988"],"issn-type":[{"value":"2235-2988","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,11,12]]},"article-number":"382"}}