{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T05:10:23Z","timestamp":1790399423311,"version":"4.1.0"},"reference-count":91,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2020,1,23]],"date-time":"2020-01-23T00:00:00Z","timestamp":1579737600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001843","name":"Science and Engineering Research Board","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001843","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100007611","name":"Shiv Nadar University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100007611","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Cell. Infect. Microbiol."],"DOI":"10.3389\/fcimb.2019.00478","type":"journal-article","created":{"date-parts":[[2020,1,23]],"date-time":"2020-01-23T05:14:15Z","timestamp":1579756455000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":21,"title":["Hepatitis E Virus Cysteine Protease Has Papain Like Properties Validated by in silico Modeling and Cell-Free Inhibition Assays"],"prefix":"10.3389","volume":"9","author":[{"given":"Shweta","family":"Saraswat","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meenakshi","family":"Chaudhary","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Deepak","family":"Sehgal","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2020,1,23]]},"reference":[{"key":"B1","volume-title":"Glide","year":"2017"},{"key":"B2","volume-title":"Ligprep.","year":"2017"},{"key":"B3","volume-title":"MacroModel.","year":"2017"},{"key":"B4","volume-title":"Maestro.","year":"2017"},{"key":"B5","volume-title":"Protein Preparation Wizard.","year":"2017"},{"key":"B6","volume-title":"SiteMap.","year":"2017"},{"key":"B7","doi-asserted-by":"publisher","first-page":"1484","DOI":"10.1111\/j.1440-1746.2009.05933.x","article-title":"Epidemiology of hepatitis E: current status","volume":"24","author":"Aggarwal","year":"2009","journal-title":"J. Gastroenterol. Hepatol."},{"key":"B8","doi-asserted-by":"publisher","first-page":"87","DOI":"10.1006\/viro.2000.0819","article-title":"The 3\u2032 end of hepatitis E virus (HEV) genome binds specifically to the viral RNA-dependent RNA polymerase (RdRp)","volume":"282","author":"Agrawal","year":"2001","journal-title":"Virology"},{"key":"B9","doi-asserted-by":"publisher","first-page":"47","DOI":"10.1016\/j.virusres.2011.02.011","article-title":"Molecular virology of hepatitis E virus","volume":"161","author":"Ahmad","year":"2011","journal-title":"Virus Res."},{"key":"B10","doi-asserted-by":"publisher","first-page":"751","DOI":"10.1016\/j.viruses.2011.02.011","article-title":"Purification and kinetic parameters characterization of an alkaline protease produced from Bacillus subtilisthrough submerged fermentation technique","volume":"12","author":"Ahmed","year":"2011","journal-title":"World Appl. Sci. J."},{"key":"B11","doi-asserted-by":"publisher","first-page":"275","DOI":"10.1002\/(SICI)1096-9071(200003)60:3<275::AID-JMV5>3.0.CO;2-9","article-title":"Cloning, sequencing, and expression of the hepatitis E virus (HEV) nonstructural open reading frame 1 (ORF1)","volume":"60","author":"Ansari","year":"2000","journal-title":"J. Med. Virol."},{"key":"B12","doi-asserted-by":"publisher","first-page":"4180","DOI":"10.1016\/j.bmcl.2005.06.091","article-title":"Hepatitis C virus NS3-4A serine protease inhibitors: SAR of P'2 moiety with improved potency","volume":"15","author":"Arasappan","year":"2005","journal-title":"Bioorg. Med. Chem. Lett."},{"key":"B13","doi-asserted-by":"publisher","first-page":"371","DOI":"10.1006\/jmbi.1999.2745","article-title":"The solution structure of the N-terminal proteinase domain of the hepatitis C virus (HCV) NS3 protein provides new insights into its activation and catalytic mechanism","volume":"289","author":"Barbato","year":"1999","journal-title":"J. Mol. Biol."},{"key":"B14","doi-asserted-by":"publisher","first-page":"7872","DOI":"10.1073\/pnas.85.21.7872","article-title":"Viral cysteine proteases are homologous to the trypsin-like family of serine proteases: structural and functional implications","volume":"85","author":"Bazan","year":"1988","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"B15","doi-asserted-by":"publisher","first-page":"95","DOI":"10.1002\/jmv.21033","article-title":"Serological response to hepatitis E virus genotype 3 infection: IgG quantitation, avidity, and IgM response","volume":"80","author":"Bendall","year":"2008","journal-title":"J. Med. Virol."},{"key":"B16","doi-asserted-by":"publisher","first-page":"1081","DOI":"10.1126\/science.271.5252.1081","article-title":"The galvanization of biology: a growing appreciation for the roles of zinc","volume":"271","author":"Berg","year":"1996","journal-title":"Science"},{"key":"B17","doi-asserted-by":"publisher","first-page":"164","DOI":"10.1126\/science.1853201","article-title":"A method to identify protein sequences that fold into a known three-dimensional structure","volume":"253","author":"Bowie","year":"1991","journal-title":"Science"},{"key":"B18","doi-asserted-by":"publisher","first-page":"e17","DOI":"10.1038\/emi.2012.7","article-title":"Molecular biology and replication of hepatitis E virus","volume":"1","author":"Cao","year":"2012","journal-title":"Emerg. Microbes Infect"},{"key":"B19","doi-asserted-by":"publisher","first-page":"5.4.1","DOI":"10.1002\/cpps.47","article-title":"Overview of the baculovirus expression system","volume":"91","author":"Chambers","year":"2018","journal-title":"Curr. Protoc. Protein Sci."},{"key":"B20","doi-asserted-by":"publisher","first-page":"7100","DOI":"10.1128\/JVI.00403-08","article-title":"The hepatitis E virus ORF3 protein modulates epidermal growth factor receptor trafficking, STAT3 translocation, and the acute-phase response","volume":"82","author":"Chandra","year":"2008","journal-title":"J. Virol."},{"key":"B21","doi-asserted-by":"publisher","first-page":"1511","DOI":"10.1002\/pro.5560020916","article-title":"Verification of protein structures: patterns of nonbonded atomic interactions","volume":"2","author":"Colovos","year":"1993","journal-title":"Protein Sci."},{"key":"B22","doi-asserted-by":"publisher","first-page":"5492","DOI":"10.1002\/j.1460-2075.1996.tb00934.x","article-title":"Structure of human procathepsin L reveals the molecular basis of inhibition by the prosegment","volume":"15","author":"Coulombe","year":"1996","journal-title":"EMBO J."},{"key":"B23","doi-asserted-by":"publisher","first-page":"200","DOI":"10.1016\/j.jhep.2016.02.045","article-title":"Update on hepatitis E virology: Implications for clinical practice","volume":"65","author":"Debing","year":"2016","journal-title":"J. Hepatol."},{"key":"B24","doi-asserted-by":"publisher","first-page":"930","DOI":"10.1086\/432488","article-title":"Thermal stability of hepatitis E virus","volume":"192","author":"Emerson","year":"2005","journal-title":"J. Infect. Dis."},{"key":"B25","doi-asserted-by":"publisher","first-page":"1739","DOI":"10.1021\/jm0306430","article-title":"Glide: a new approach for rapid, accurate docking and scoring. Method and Assessment of Docking Accuracy","volume":"7","author":"Friesner","year":"2004","journal-title":"J. Med. Chem"},{"key":"B26","doi-asserted-by":"publisher","first-page":"6177","DOI":"10.1021\/jm051256o","article-title":"Extra precision glide: docking and scoring incorporating a model of hydrophobic enclosure for protein-ligand complexes","volume":"49","author":"Friesner","year":"2006","journal-title":"J. Med. Chem."},{"key":"B27","doi-asserted-by":"publisher","first-page":"103","DOI":"10.1016\/0014-5793(89)80109-7","article-title":"Cysteine proteases of positive strand RNA viruses and chymotrypsin-like serine proteases","volume":"243","author":"Gorbalenya","year":"1989","journal-title":"A distinct protein superfamily with a common structural fold. FEBS Lett."},{"key":"B28","doi-asserted-by":"publisher","first-page":"201","DOI":"10.1016\/0014-5793(91)81034-6","article-title":"Putative papain-related thiol proteases of positive-strand RNA viruses. Identification of rubi- and aphthovirus proteases and delineation of a novel conserved domain associated with proteases of rubi-, alpha- and coronaviruses","volume":"288","author":"Gorbalenya","year":"1991","journal-title":"FEBS Lett"},{"key":"B29","doi-asserted-by":"publisher","first-page":"6680","DOI":"10.1128\/JVI.79.11.6680-6689.2005","article-title":"The open reading frame 3 gene of hepatitis E virus contains a cis-reactive element and encodes a protein required for infection of macaques","volume":"79","author":"Graff","year":"2005","journal-title":"J. Virol"},{"key":"B30","doi-asserted-by":"publisher","first-page":"1227","DOI":"10.1006\/jmbi.2000.4115","article-title":"Structural and biochemical features distinguish the foot-and-mouth disease virus leader proteinase from other papain-like enzymes","volume":"302","author":"Guarne","year":"2000","journal-title":"J. Mol. Biol."},{"key":"B31","doi-asserted-by":"publisher","first-page":"317","DOI":"10.4103\/ijmm.IJMM_18_149","article-title":"Hepatitis E virus infection: An old virus with a new story!","volume":"36","author":"Gupta","year":"2018","journal-title":"Ind. J. Med. Microbiol."},{"key":"B32","doi-asserted-by":"publisher","first-page":"146","DOI":"10.1111\/j.1747-0285.2007.00483.x","article-title":"New method for fast and accurate binding-site identification and analysis","volume":"69","author":"Halgren","year":"2007","journal-title":"Chem. Biol. Drug Des."},{"key":"B33","doi-asserted-by":"publisher","first-page":"377","DOI":"10.1021\/ci800324m","article-title":"Identifying and characterizing binding sites and assessing druggability","volume":"49","author":"Halgren","year":"2009","journal-title":"J. Chem. Inf. Model."},{"key":"B34","doi-asserted-by":"publisher","first-page":"1750","DOI":"10.1021\/jm030644s","article-title":"Glide: a new approach for rapid, accurate docking and scoring. 2. Enrichment factors in database screening","volume":"47","author":"Halgren","year":"2004","journal-title":"J. Med. Chem."},{"key":"B35","doi-asserted-by":"publisher","first-page":"14918","DOI":"10.1074\/jbc.274.21.14918","article-title":"A human RNA viral cysteine proteinase that depends upon a unique Zn2+-binding finger connecting the two domains of a papain-like fold","volume":"274","author":"Herold","year":"1999","journal-title":"J. Biol. Chem."},{"key":"B36","doi-asserted-by":"publisher","first-page":"116","DOI":"10.1016\/j.febslet.2004.08.015","article-title":"Evaluation of metal-conjugated compounds as inhibitors of 3CL protease of SARS-CoV","volume":"574","author":"Hsu","year":"2004","journal-title":"FEBS Lett"},{"key":"B37","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1017\/S1462399499001271","article-title":"Molecular biology and pathogenesis of hepatitis E virus","volume":"1999","author":"Jameel","year":"1999","journal-title":"Exp. Rev. Mol. Med."},{"key":"B38","doi-asserted-by":"publisher","first-page":"4225","DOI":"10.1128\/AEM.00951-16","article-title":"Thermal stability of hepatitis e virus as estimated by a cell culture method","volume":"82","author":"Johne","year":"2016","journal-title":"Appl. Environ. Microbiol."},{"key":"B39","doi-asserted-by":"publisher","first-page":"116","DOI":"10.1128\/CMR.00057-13","article-title":"Hepatitis E virus infection","volume":"27","author":"Kamar","year":"2014","journal-title":"Clin Microbiol. Rev"},{"key":"B40","doi-asserted-by":"publisher","first-page":"e01853","DOI":"10.1128\/JVI.01853-17","article-title":"Activities of thrombin and factor Xa are essential for replication of hepatitis E virus and are possibly implicated in ORF1 polyprotein processing","volume":"92","author":"Kanade","year":"2018","journal-title":"J. Virol."},{"key":"B41","doi-asserted-by":"publisher","first-page":"3595","DOI":"10.1128\/JVI.02130-09","article-title":"NTPase and 5\u2032 to 3\u2032 RNA duplex-unwinding activities of the hepatitis E virus helicase domain","volume":"84","author":"Karpe","year":"","journal-title":"J. Virol"},{"key":"B42","doi-asserted-by":"publisher","first-page":"9637","DOI":"10.1128\/JVI.00492-10","article-title":"RNA 5\u2032-triphosphatase activity of the hepatitis E virus helicase domain","volume":"84","author":"Karpe","year":"","journal-title":"J. Virol"},{"key":"B43","doi-asserted-by":"publisher","first-page":"87","DOI":"10.1016\/0168-1702(95)00018-L","article-title":"Adenovirus protease expressed in insect cells cleaves adenovirus proteins, ovalbumin and baculovirus protease in the absence of activating peptide","volume":"37","author":"Keyvani-Amineh","year":"1995","journal-title":"Virus Res"},{"key":"B44","doi-asserted-by":"publisher","first-page":"8259","DOI":"10.1073\/pnas.89.17.8259","article-title":"Computer-assisted assignment of functional domains in the nonstructural polyprotein of hepatitis E virus: delineation of an additional group of positive-strand RNA plant and animal viruses","volume":"89","author":"Koonin","year":"1992","journal-title":"Proc. Natl. Acad. Sci. U.S.A"},{"key":"B45","doi-asserted-by":"publisher","first-page":"226","DOI":"10.1186\/1756-0500-3-226","article-title":"EasyModeller: a graphical interface to MODELLER","volume":"3","author":"Kuntal","year":"2010","journal-title":"BMC Res. Notes"},{"key":"B46","doi-asserted-by":"publisher","first-page":"283","DOI":"10.1107\/S0021889892009944","article-title":"PROCHECK: a program to check the stereochemical quality of protein structures","volume":"26","author":"Laskowski","year":"1993","journal-title":"J. Appl. Cryst."},{"key":"B47","doi-asserted-by":"crossref","first-page":"4463","DOI":"10.1128\/JVI.72.5.4463-4466.1998","article-title":"The rubella virus nonstructural protease requires divalent cations for activity and functions in trans","volume":"72","author":"Liu","year":"1998","journal-title":"J. Virol."},{"key":"B48","doi-asserted-by":"publisher","first-page":"e369","DOI":"10.1371\/journal.pntd.0000369","article-title":"The importance of pH in regulating the function of the Fasciola hepatica cathepsin L1 cysteine protease","volume":"3","author":"Lowther","year":"2009","journal-title":"PLoS Negl. Trop. Dis."},{"key":"B49","doi-asserted-by":"publisher","first-page":"83","DOI":"10.1038\/356083a0","article-title":"Assessment of protein models with three-dimensional profiles","volume":"356","author":"L\u00fcthy","year":"1992","journal-title":"Nature"},{"key":"B50","doi-asserted-by":"publisher","first-page":"95","DOI":"10.2147\/HIV.S79956","article-title":"HIV protease inhibitors: a review of molecular selectivity and toxicity","volume":"7","author":"Lv","year":"2015","journal-title":"HIV AIDS"},{"key":"B51","doi-asserted-by":"publisher","first-page":"6249","DOI":"10.1128\/JVI.75.14.6249-6255.2001","article-title":"Virus-specific mRNA capping enzyme encoded by hepatitis E virus","volume":"75","author":"Magden","year":"2001","journal-title":"J. Virol."},{"key":"B52","doi-asserted-by":"publisher","first-page":"761","DOI":"10.1016\/0092-8674(94)90059-0","article-title":"Structure of human rhinovirus 3C protease reveals a trypsin-like polypeptide fold, RNA-binding site, and means for cleaving precursor polyprotein","volume":"77","author":"Matthews","year":"1994","journal-title":"Cell"},{"key":"B53","doi-asserted-by":"publisher","first-page":"408","DOI":"10.1093\/nar\/gkz322","article-title":"IntFOLD: an integrated web resource for high performance protein structure and function prediction","volume":"2","author":"McGuffin","year":"2019","journal-title":"Nucleic Acids Res"},{"key":"B54","doi-asserted-by":"publisher","first-page":"69","DOI":"10.1093\/nar\/gkv236","article-title":"IntFOLD: an integrated server for modelling protein structures and functions from amino acid sequences","volume":"1","author":"McGuffin","year":"2015","journal-title":"Nucleic Acids Res."},{"key":"B55","first-page":"333","article-title":"Die Kinetik der Invertinwirkung","volume":"49","author":"Michaelis","year":"1913","journal-title":"Biochem. Z."},{"key":"B56","doi-asserted-by":"publisher","first-page":"439","DOI":"10.1155\/2007\/289059","article-title":"Serological evidence of hepatitis E virus infection in an indigenous North American population","volume":"21","author":"Minuk","year":"2007","journal-title":"Can. J. Gastroenterol."},{"key":"B57","doi-asserted-by":"publisher","first-page":"646","DOI":"10.1002\/jmv.21116","article-title":"Hepatitis E virus: molecular virology, clinical features, diagnosis, transmission, epidemiology, and prevention","volume":"80","author":"Mushahwar","year":"2008","journal-title":"J. Med. Virol."},{"key":"B58","doi-asserted-by":"publisher","first-page":"e1005521","DOI":"10.1371\/journal.ppat.1005521","article-title":"Endoplasmic reticulum stress induced synthesis of a novel viral factor mediates efficient replication of genotype-1 hepatitis E virus","volume":"12","author":"Nair","year":"2016","journal-title":"PLoS Pathog."},{"key":"B59","doi-asserted-by":"publisher","first-page":"1419","DOI":"10.3389\/fmicb.2016.01419","article-title":"Molecular biology and infection of hepatitis E virus","volume":"7","author":"Nan","year":"2016","journal-title":"Front. Microbiol"},{"key":"B60","doi-asserted-by":"publisher","first-page":"1190","DOI":"10.1111\/j.1478-3231.2008.01840.x","article-title":"Hepatitis E and pregnancy: understanding the pathogenesis","volume":"28","author":"Navaneethan","year":"2008","journal-title":"Liver Int."},{"key":"B61","doi-asserted-by":"publisher","first-page":"350","DOI":"10.1126\/science.6369538","article-title":"Evolution of proteolytic enzymes","volume":"224","author":"Neurath","year":"1984","journal-title":"Science"},{"key":"B62","doi-asserted-by":"publisher","first-page":"1689","DOI":"10.1099\/vir.0.066142-0","article-title":"Hepatitis E virus (HEV) protease: a chymotrypsin-like enzyme that processes both non-structural (pORF1) and capsid (pORF2) protein","volume":"95","author":"Paliwal","year":"2014","journal-title":"J. Gen. Virol."},{"key":"B63","doi-asserted-by":"publisher","first-page":"2430","DOI":"10.1128\/JVI.74.5.2430-2437.2000","article-title":"The in vitro-synthesized RNA from a cDNA clone of hepatitis E virus is infectious","volume":"74","author":"Panda","year":"2000","journal-title":"J. Virol."},{"key":"B64","doi-asserted-by":"publisher","first-page":"553","DOI":"10.1016\/j.virusres.2013.07.020","article-title":"Molecular characterization of hepatitis E virus ORF1 gene supports a papain-like cysteine protease (PCP)-domain activity","volume":"178","author":"Parvez","year":"2013","journal-title":"Virus Res."},{"key":"B65","doi-asserted-by":"publisher","first-page":"915","DOI":"10.2217\/fmb-2017-0016","article-title":"The hepatitis E virus nonstructural polyprotein","volume":"12","author":"Parvez","year":"2017","journal-title":"Future Microbiol."},{"key":"B66","doi-asserted-by":"publisher","first-page":"220","DOI":"10.1016\/j.virusres.2013.11.016","article-title":"Molecular modeling and analysis of hepatitis E virus (HEV) papain-like cysteine protease","volume":"179","author":"Parvez","year":"2014","journal-title":"Virus Res"},{"key":"B67","doi-asserted-by":"publisher","first-page":"1673","DOI":"10.1128\/CVI.05258-11","article-title":"Expression and solubilization of insect cell-based rabies virus glycoprotein and assessment of its immunogenicity and protective efficacy in mice","volume":"18","author":"Ramya","year":"2011","journal-title":"Clin. Vaccine Immunol."},{"key":"B68","doi-asserted-by":"publisher","first-page":"1583","DOI":"10.1104\/pp.112.194001","article-title":"Subclassification and biochemical analysis of plant papain-like cysteine proteases displays subfamily-specific characteristics","volume":"158","author":"Richau","year":"2012","journal-title":"Plant Physiol."},{"key":"B69","doi-asserted-by":"publisher","first-page":"171","DOI":"10.1093\/nar\/gkr184","article-title":"The IntFOLD server: an integrated web resource for protein fold recognition, 3D model quality assessment, intrinsic disorder prediction, domain prediction and ligand binding site prediction","volume":"39","author":"Roche","year":"2011","journal-title":"Nucleic Acids Res."},{"key":"B70","doi-asserted-by":"publisher","first-page":"1321","DOI":"10.1007\/s007050070093","article-title":"Expression of the hepatitis E virus ORF1","volume":"145","author":"Ropp","year":"2000","journal-title":"Arch. Virol"},{"key":"B71","doi-asserted-by":"publisher","first-page":"725","DOI":"10.1038\/nprot.2010.5","article-title":"I-TASSER: a unified platform for automated protein structure and function prediction","volume":"5","author":"Roy","year":"2010","journal-title":"Nat. Protoc."},{"key":"B72","doi-asserted-by":"publisher","first-page":"10831","DOI":"10.1038\/s41598-018-29024-2","article-title":"Development of nsP2 protease based cell free high throughput screening assay for evaluation of inhibitors against emerging Chikungunya virus","volume":"8","author":"Saha","year":"2018","journal-title":"Sci. Rep."},{"key":"B73","doi-asserted-by":"publisher","first-page":"51","DOI":"10.4137\/117739010700200011","article-title":"Identification of cysteine proteases and screening of cysteine protease inhibitors in biological samples by a two-dimensional gel system of zymography and reverse zymography","volume":"2","author":"Saitoh","year":"2007","journal-title":"Anal. Chem. Insights"},{"key":"B74","doi-asserted-by":"publisher","first-page":"3392","DOI":"10.1021\/bi972010r","article-title":"Serine protease of hepatitis C virus expressed in insect cells as the NS3\/4A complex","volume":"37","author":"Sali","year":"1998","journal-title":"Biochemistry"},{"key":"B75","doi-asserted-by":"publisher","first-page":"221","DOI":"10.1007\/s10822-013-9644-8","article-title":"Protein and ligand preparation: parameters, protocols, and influence on virtual screening enrichments","volume":"27","author":"Sastry","year":"2013","journal-title":"J. Comput. Aid Mol. Des."},{"key":"B76","doi-asserted-by":"publisher","first-page":"38","DOI":"10.1186\/1743-422X-3-38","article-title":"Expression and processing of the Hepatitis E virus ORF1 nonstructural polyprotein","volume":"3","author":"Sehgal","year":"2006","journal-title":"Virol. J."},{"key":"B77","doi-asserted-by":"crossref","first-page":"2881","DOI":"10.1128\/JVI.71.4.2881-2886.1997","article-title":"The NS3 proteinase domain of hepatitis C virus is a zinc-containing enzyme","volume":"71","author":"Stempniak","year":"1997","journal-title":"J. Virol."},{"key":"B78","doi-asserted-by":"publisher","first-page":"e47227","DOI":"10.1371\/journal.pone.0047227","article-title":"The ionic and hydrophobic interactions are required for the auto activation of cysteine proteases of Plasmodium falciparum","volume":"7","author":"Sundararaj","year":"2012","journal-title":"PLoS ONE"},{"key":"B79","doi-asserted-by":"publisher","first-page":"120","DOI":"10.1016\/0042-6822(91)90760-9","article-title":"Hepatitis E virus (HEV): molecular cloning and sequencing of the full-length viral genome","volume":"185","author":"Tam","year":"1991","journal-title":"Virology"},{"key":"B80","doi-asserted-by":"publisher","first-page":"903","DOI":"10.1099\/vir.0.82535-0","article-title":"Development and evaluation of an efficient cell-culture system for hepatitis E virus","volume":"88","author":"Tanaka","year":"2007","journal-title":"J. Gen. Virol."},{"key":"B81","doi-asserted-by":"publisher","first-page":"559","DOI":"10.1073\/pnas.89.2.559","article-title":"Characterization of a prototype strain of hepatitis E virus","volume":"89","author":"Tsarev","year":"1992","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"B82","doi-asserted-by":"publisher","first-page":"661","DOI":"10.1038\/90273","article-title":"Determination of protease cleavage site motifs using mixture-based oriented peptide libraries","volume":"19","author":"Turk","year":"2001","journal-title":"Nat. Biotechnol."},{"key":"B83","doi-asserted-by":"publisher","first-page":"30","DOI":"10.1016\/0003-2697(84)90553-0","article-title":"Fluorescein isothiocyanate-labeled casein assay for proteolytic enzymes","volume":"143","author":"Twining","year":"1984","journal-title":"Anal. Biochem."},{"key":"B84","doi-asserted-by":"publisher","first-page":"18760","DOI":"10.1074\/jbc.273.30.18760","article-title":"The metal binding site of the hepatitis C virus NS3 protease. A spectroscopic investigation","volume":"273","author":"Urbani","year":"1998","journal-title":"J. Biol. Chem."},{"key":"B85","doi-asserted-by":"publisher","first-page":"107","DOI":"10.3389\/fphar.2016.00107","article-title":"Cysteine proteases: modes of activation and future prospects as pharmacological targets","volume":"7","author":"Verma","year":"2016","journal-title":"Front. Pharmacol."},{"key":"B86","doi-asserted-by":"publisher","first-page":"1880","DOI":"10.1099\/vir.0.010561-0","article-title":"ORF3 protein of hepatitis E virus is essential for virion release from infected cells","volume":"90","author":"Yamada","year":"2009","journal-title":"J. Gen. Virol."},{"key":"B87","doi-asserted-by":"publisher","first-page":"7","DOI":"10.1038\/nmeth.3213","article-title":"The I-TASSER suite: protein structure and function prediction","volume":"12","author":"Yang","year":"2015","journal-title":"Nat. Methods"},{"key":"B88","doi-asserted-by":"publisher","first-page":"725","DOI":"10.1016\/0042-6822(92)90039-R","article-title":"Characterization of the roles of conserved cysteine and histidine residues in poliovirus 2A protease","volume":"186","author":"Yu","year":"1992","journal-title":"Virology"},{"key":"B89","doi-asserted-by":"publisher","first-page":"40","DOI":"10.1186\/1471-2105-9-40","article-title":"I-TASSER server for protein 3D structure prediction","volume":"9","author":"Zhang","year":"2008","journal-title":"BMC Bioinformatics"},{"key":"B90","doi-asserted-by":"publisher","first-page":"7517","DOI":"10.1128\/JVI.00605-07","article-title":"Identification of a Ca2+-binding domain in the rubella virus nonstructural protease","volume":"81","author":"Zhou","year":"2007","journal-title":"J. Virol."},{"key":"B91","doi-asserted-by":"publisher","first-page":"3922","DOI":"10.1128\/JVI.02091-06","article-title":"Human coronavirus 229E papain-like proteases have overlapping specificities but distinct functions in viral replication","volume":"81","author":"Ziebuhr","year":"2007","journal-title":"J. Virol."}],"container-title":["Frontiers in Cellular and Infection Microbiology"],"original-title":[],"link":[{"URL":"https:\/\/www.frontiersin.org\/article\/10.3389\/fcimb.2019.00478\/full","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,1,23]],"date-time":"2020-01-23T05:14:19Z","timestamp":1579756459000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.frontiersin.org\/article\/10.3389\/fcimb.2019.00478\/full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,1,23]]},"references-count":91,"alternative-id":["10.3389\/fcimb.2019.00478"],"URL":"https:\/\/doi.org\/10.3389\/fcimb.2019.00478","relation":{},"ISSN":["2235-2988"],"issn-type":[{"value":"2235-2988","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,1,23]]},"article-number":"478"}}