{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T16:18:06Z","timestamp":1759335486651,"version":"3.41.2"},"reference-count":52,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2020,2,4]],"date-time":"2020-02-04T00:00:00Z","timestamp":1580774400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Cell. Infect. Microbiol."],"DOI":"10.3389\/fcimb.2020.00029","type":"journal-article","created":{"date-parts":[[2020,2,4]],"date-time":"2020-02-04T04:30:12Z","timestamp":1580790612000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":6,"title":["Screening the Drug:H+ Antiporter Family for a Role in Biofilm Formation in Candida glabrata"],"prefix":"10.3389","volume":"10","author":[{"given":"Rui","family":"Santos","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mafalda","family":"Cavalheiro","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Catarina","family":"Costa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Azusa","family":"Takahashi-Nakaguchi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michiyo","family":"Okamoto","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiroji","family":"Chibana","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Miguel C.","family":"Teixeira","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1965","published-online":{"date-parts":[[2020,2,4]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"195","DOI":"10.1042\/BJ20110876","article-title":"The yeast ABC transporter Pdr18 (ORF YNR070w) controls plasma membrane sterol composition, playing a role in multidrug resistance","volume":"440","author":"Cabrito","year":"2011","journal-title":"Biochem. J."},{"key":"B2","doi-asserted-by":"publisher","first-page":"425","DOI":"10.1111\/omi.12109","article-title":"Learning the ABC of oral fungal drug resistance","volume":"30","author":"Cannon","year":"2015","journal-title":"Mol. Oral Microbiol."},{"key":"B3","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1128\/AAC.00995-18","article-title":"A transcriptomics approach to unveiling the mechanisms of in vitro evolution towards fluconazole resistance of a Candida glabrata clinical isolate","volume":"63","author":"Cavalheiro","year":"2019","journal-title":"Antimicrob. Agents Chemother."},{"key":"B4","doi-asserted-by":"publisher","first-page":"332","DOI":"10.3390\/genes9070332","article-title":"Host-pathogen interactions mediated by MDR transporters in fungi: as pleiotropic as it gets!","volume":"9","author":"Cavalheiro","year":"2018","journal-title":"Genes"},{"key":"B5","doi-asserted-by":"publisher","first-page":"28","DOI":"10.3389\/fmed.2018.00028","article-title":"Candida biofilms: threats, challenges, and promising strategies","volume":"5","author":"Cavalheiro","year":"2018","journal-title":"Front. Med."},{"key":"B6","doi-asserted-by":"publisher","first-page":"197","DOI":"10.3389\/fphys.2014.00197","article-title":"MFS multidrug transporters in pathogenic fungi: do they have real clinical impact?","volume":"5","author":"Costa","year":"","journal-title":"Front. Physiol."},{"key":"B7","doi-asserted-by":"publisher","first-page":"170","DOI":"10.3389\/fmicb.2013.00170","article-title":"The dual role of Candida glabrata drug:H+ antiporter CgAqr1 (ORF CAGL0J09944g) in antifungal drug and acetic acid resistance","volume":"4","author":"Costa","year":"","journal-title":"Front. Microbiol."},{"key":"B8","doi-asserted-by":"publisher","first-page":"1767","DOI":"10.1093\/jac\/dku044","article-title":"Candida glabrata drug:H+ antiporter CgTpo3 (ORF CAGL0I10384g): role in azole drug resistance and polyamine homeostasis","volume":"69","author":"Costa","year":"","journal-title":"J. Antimicrob. Chemother."},{"key":"B9","doi-asserted-by":"publisher","first-page":"3159","DOI":"10.1128\/AAC.00811-12","article-title":"Candida glabrata drug: H+ antiporter CgQdr2 confers imidazole drug resistance, being activated by transcription factor CgPdr1","volume":"57","author":"Costa","year":"","journal-title":"Antimicrob. Agents Chemother."},{"key":"B10","doi-asserted-by":"publisher","first-page":"526","DOI":"10.3389\/fmicb.2016.00526","article-title":"Clotrimazole drug resistance in Candida glabrata clinical isolates correlates with increased expression of the drug:H+ antiporters CgAqr1, CgTpo1_1, CgTpo3 and CgQdr2","volume":"7","author":"Costa","year":"2016","journal-title":"Front. Microbiol."},{"key":"B11","doi-asserted-by":"publisher","first-page":"228","DOI":"10.1128\/EC.00224-14","article-title":"Role of Tec1 in the development, architecture, and integrity of sexual biofilms of Candida albicans","volume":"14","author":"Daniels","year":"2015","journal-title":"Eukaryotic Cell"},{"key":"B12","doi-asserted-by":"publisher","first-page":"470","DOI":"10.1128\/EC.00364-12","article-title":"Adhesins in human fungal pathogens: glue with plenty of stick","volume":"12","author":"de Groot","year":"2013","journal-title":"Eukaryotic Cell"},{"key":"B13","doi-asserted-by":"publisher","first-page":"125","DOI":"10.1042\/bj3360125","article-title":"Proton stoichiometry of the overexpressed uracil symport of the yeast Saccharomyces cerevisiae","volume":"336","author":"Eddy","year":"1998","journal-title":"Biochem. J."},{"key":"B14","doi-asserted-by":"publisher","first-page":"437","DOI":"10.1093\/cid\/cir110","article-title":"Candida urinary tract infection: pathogenesis","volume":"52","author":"Fisher","year":"2011","journal-title":"Clin. Infect. Dis."},{"key":"B15","doi-asserted-by":"publisher","first-page":"iv48","DOI":"10.1093\/jac\/dkz287","article-title":"Species distribution and antifungal susceptibility of invasive Candida isolates from Canadian hospitals: results of the CANWARD 2011\u201316 study","volume":"74","author":"Fuller","year":"2019","journal-title":"J. Antimicrob. Chemother."},{"key":"B16","doi-asserted-by":"publisher","first-page":"245","DOI":"10.1016\/j.mycmed.2019.07.006","article-title":"The epidemiology of Candida species in the middle east and north Africa","volume":"29","author":"Ghazi","year":"2019","journal-title":"J. Mycol. Med."},{"key":"B17","doi-asserted-by":"publisher","first-page":"905","DOI":"10.3109\/1040841X.2015.1091805","article-title":"Vulvovaginal candidiasis: epidemiology, microbiology and risk factors","volume":"42","author":"Gon\u00e7alves","year":"2016","journal-title":"Crit. Rev. Microbiol."},{"key":"B18","doi-asserted-by":"publisher","first-page":"7654","DOI":"10.1128\/MCB.20.20.7654-7661.2000","article-title":"Regulation of yeast H(+)-ATPase by protein kinases belonging to a family dedicated to activation of plasma membrane transporters","volume":"20","author":"Goossens","year":"2000","journal-title":"Mol. Cell. Biol."},{"key":"B19","doi-asserted-by":"publisher","first-page":"398","DOI":"10.1016\/0076-6879(91)94030-G","article-title":"Preparation of high molecular weight RNA","volume":"194","author":"K\u00f6hrer","year":"1991","journal-title":"Meth. Enzymol."},{"key":"B20","doi-asserted-by":"publisher","first-page":"846","DOI":"10.1093\/jac\/dku447","article-title":"In vivo Candida glabrata biofilm development on foreign bodies in a rat subcutaneous model","volume":"70","author":"Kucharikova","year":"2014","journal-title":"J. Antimicrob. Chemother."},{"key":"B21","doi-asserted-by":"publisher","first-page":"1261","DOI":"10.1099\/jmm.0.032037-0","article-title":"Detailed comparison of Candida albicans and Candida glabrata biofilms under different conditions and their susceptibility to caspofungin and anidulafungin","volume":"60","author":"Kuchar\u00edkovA","year":"2011","journal-title":"J. Med. Microbiol."},{"key":"B22","doi-asserted-by":"publisher","first-page":"824","DOI":"10.1080\/23744235.2019.1662941","article-title":"Clinical and microbiological factors associated with mortality in candidemia in adult patients 2007\u20132016","volume":"51","author":"Mari","year":"2019","journal-title":"Infect. Dis."},{"key":"B23","first-page":"664","article-title":"The Candida albicans AAA ATPase homologue of Saccharomyces cerevisiae Rix7p (YLL034c) is essential for proper morphology, biofilm formation and activity of secreted aspartyl proteinases","volume":"5","author":"Melo","year":"2006","journal-title":"Genet. Mol. Res."},{"key":"B24","doi-asserted-by":"publisher","first-page":"103","DOI":"10.1007\/s00294-018-0870-4","article-title":"Multiple interfaces control activity of the Candida glabrata Pdr1 transcription factor mediating azole drug resistance","volume":"65","author":"Moye-Rowley","year":"2019","journal-title":"Curr. Genet."},{"key":"B25","doi-asserted-by":"publisher","first-page":"126","DOI":"10.1016\/j.cell.2011.10.048","article-title":"A recently evolved transcriptional network controls biofilm development in Candida albicans","volume":"148","author":"Nobile","year":"2012","journal-title":"Cell"},{"key":"B26","doi-asserted-by":"publisher","first-page":"8","DOI":"10.1186\/s12866-019-1388-8","article-title":"Vaginal pH measured in vivo: lactobacilli determine pH and lactic acid concentration","volume":"19","author":"O'Hanlon","year":"2019","journal-title":"BMC Microbiol."},{"key":"B27","doi-asserted-by":"publisher","first-page":"91","DOI":"10.1016\/S0010-7824(99)00010-4","article-title":"A vaginal fluid simulant","volume":"59","author":"Owen","year":"1999","journal-title":"Contraception"},{"key":"B28","doi-asserted-by":"publisher","first-page":"57","DOI":"10.1074\/mcp.M114.045344","article-title":"Membrane proteome-wide response to the antifungal drug clotrimazole in Candida glabrata: role of the transcription factor CgPdr1 and the drug:H+ antiporters CgTpo1_1 and CgTpo1_2","volume":"15","author":"Pais","year":"","journal-title":"Mol. Cell. Proteomics"},{"key":"B29","doi-asserted-by":"publisher","first-page":"155","DOI":"10.1007\/978-3-030-13035-0_7","article-title":"Genome-wide response to drugs and stress in the pathogenic yeast candida glabrata","volume":"58","author":"Pais","year":"2019","journal-title":"Prog. Mol. Subcell. Biol."},{"key":"B30","doi-asserted-by":"publisher","first-page":"2045","DOI":"10.3389\/fmicb.2016.02045","article-title":"Membrane proteomics analysis of the Candida glabrata response to 5-flucytosine: unveiling the role and regulation of the drug efflux transporters CgFlr1 and CgFlr2","volume":"7","author":"Pais","year":"","journal-title":"Front. Microbiol."},{"key":"B31","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1080\/20002297.2017.1385372","article-title":"Inactivation of genes TEC1 and EFG1 in Candida albicans influences extracellular matrix composition and biofilm morphology","volume":"9","author":"Panariello","year":"2017","journal-title":"J. Oral Microbiol."},{"key":"B32","doi-asserted-by":"publisher","first-page":"1","DOI":"10.3390\/microorganisms7090285","article-title":"The varied role of efflux pumps of the MFS family in the interplay of bacteria with animal and plant cells","volume":"7","author":"Pasqua","year":"2019","journal-title":"Microorganisms"},{"key":"B33","doi-asserted-by":"publisher","first-page":"70","DOI":"10.1590\/S1678-77572012000100013","article-title":"Multi-species biofilm of Candida albicans and non-Candida albicans Candida species on acrylic substrate","volume":"20","author":"Pathak","year":"2012","journal-title":"J. Appl. Oral Sci."},{"key":"B34","doi-asserted-by":"publisher","first-page":"6904","DOI":"10.1128\/AAC.03921-14","article-title":"Identification of genomic binding sites for Candida glabrata Pdr1 transcription factor in wild-type and \u03c1 0 cells","volume":"58","author":"Paul","year":"2014","journal-title":"Antimicrob. Agents Chemother."},{"key":"B35","doi-asserted-by":"publisher","first-page":"297","DOI":"10.1016\/j.resmic.2012.02.005","article-title":"New regulators of biofilm development in Candida glabrata","volume":"163","author":"Riera","year":"2012","journal-title":"Res. Microbiol."},{"key":"B36","doi-asserted-by":"publisher","first-page":"473","DOI":"10.3389\/fcimb.2017.00473","article-title":"A new determinant of Candida glabrata virulence: the acetate exporter CgDtr1","volume":"7","author":"Rom\u00e3o","year":"2017","journal-title":"Front. Cell. Infect. Microbiol."},{"key":"B37","doi-asserted-by":"publisher","first-page":"22","DOI":"10.1016\/j.tim.2008.09.007","article-title":"Drug:H+ antiporters in chemical stress response in yeast","volume":"17","author":"S\u00e1-Correia","year":"2008","journal-title":"Trends Microbiol."},{"key":"B38","doi-asserted-by":"publisher","first-page":"1029","DOI":"10.1111\/j.1567-1364.2009.00578.x","article-title":"Antifungal drug resistance mechanisms in fungal pathogens from the perspective of transcriptional gene regulation","volume":"9","author":"Sanglard","year":"2009","journal-title":"FEMS Yeast Res."},{"key":"B39","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1111\/cmi.12686","article-title":"The multidrug resistance transporters CgTpo1 _ 1 and CgTpo1 _ 2 play a role in virulence and biofilm formation in the human pathogen Candida glabrata","volume":"19","author":"Santos","year":"2017","journal-title":"Cell. Microbiol."},{"key":"B40","doi-asserted-by":"publisher","first-page":"180","DOI":"10.3389\/fphys.2014.00180","article-title":"MFS transporters required for multidrug\/multixenobiotic (MD\/MX) resistance in the model yeast: understanding their physiological function through post-genomic approaches","volume":"5","author":"Santos","year":"2014","journal-title":"Front. Physiol."},{"key":"B41","doi-asserted-by":"publisher","first-page":"2","DOI":"10.1046\/j.1365-2958.2000.02132.x","article-title":"The TEA\/ATTS transcription factor CaTec1p regulates hyphal development and virulence in Candida albicans","volume":"38","author":"Schweizer","year":"2000","journal-title":"Mol. Microbiol."},{"key":"B42","doi-asserted-by":"publisher","first-page":"223","DOI":"10.1042\/BJ20140010","article-title":"Novel role of a family of major facilitator transporters in biofilm development and virulence of Candida albicans","volume":"460","author":"Shah","year":"2014","journal-title":"Biochem. J."},{"key":"B43","doi-asserted-by":"publisher","first-page":"945","DOI":"10.1099\/mic.0.043661-0","article-title":"Yeast response and tolerance to polyamine toxicity involving the drug: H+ antiporter Qdr3 and the transcription factors Yap1 and Gcn4","volume":"157","author":"Teixeira","year":"2011","journal-title":"Microbiology"},{"key":"B44","doi-asserted-by":"publisher","first-page":"681","DOI":"10.1042\/bj3530681","article-title":"Multiple polyamine transport systems on the vacuolar membrane in yeast","volume":"353","author":"Tomitori","year":"2001","journal-title":"Biochem. J."},{"key":"B45","doi-asserted-by":"publisher","first-page":"169","DOI":"10.3390\/ph4010169","article-title":"Pathogenesis and antifungal drug resistance of the human fungal pathogen Candida glabrata","volume":"4","author":"Tscherner","year":"2011","journal-title":"Pharmaceuticals"},{"key":"B46","doi-asserted-by":"publisher","first-page":"1239","DOI":"10.1128\/EC.00414-06","article-title":"Development of a highly efficient gene targeting system induced by transient repression of YKU80 expression in Candida glabrata","volume":"6","author":"Ueno","year":"2007","journal-title":"Eukaryotic Cell"},{"key":"B47","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1128\/mBio.01338-18","article-title":"Candida albicans dispersed cells are developmentally distinct from biofilm and planktonic cells","volume":"9","author":"Uppuluri","year":"2018","journal-title":"mBio"},{"key":"B48","doi-asserted-by":"publisher","first-page":"134","DOI":"10.1128\/EC.00290-06","article-title":"Saccharomyces cerevisiae multidrug resistance transporter Qdr2 is implicated in potassium uptake, providing a physiological advantage to quinidine-stressed cells","volume":"6","author":"Vargas","year":"2007","journal-title":"Eukaryotic Cell"},{"key":"B49","doi-asserted-by":"publisher","first-page":"5","DOI":"10.1111\/j.1365-2958.2006.05072.x","article-title":"Flocculation, adhesion and biofilm formation in yeasts","volume":"60","author":"Verstrepen","year":"2006","journal-title":"Mol. Microbiol"},{"key":"B50","doi-asserted-by":"publisher","first-page":"e01070","DOI":"10.1128\/AAC.01070-18","article-title":"Relative contribution of the ABC transporters Cdr1, Pdh1, and Snq2 to azole resistance in Candida glabrata","volume":"62","author":"Whaley","year":"2018","journal-title":"Antimicrob. Agents Chemother."},{"key":"B51","doi-asserted-by":"publisher","first-page":"1154","DOI":"10.1111\/myc.13005","article-title":"Role of major facilitator superfamily transporter Qdr2p in biofilm formation by Candida glabrata","volume":"62","author":"Widiasih Widiyanto","year":"2019","journal-title":"Mycoses"},{"key":"B52","doi-asserted-by":"publisher","first-page":"e0159692","DOI":"10.1371\/journal.pone.0159692","article-title":"Transcription factors Efg1 and Bcr1 regulate biofilm formation and virulence during Candida albicans-associated denture stomatitis","volume":"11","author":"Yano","year":"2016","journal-title":"PLoS ONE"}],"container-title":["Frontiers in Cellular and Infection Microbiology"],"original-title":[],"link":[{"URL":"https:\/\/www.frontiersin.org\/article\/10.3389\/fcimb.2020.00029\/full","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,2,4]],"date-time":"2020-02-04T04:30:14Z","timestamp":1580790614000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.frontiersin.org\/article\/10.3389\/fcimb.2020.00029\/full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,2,4]]},"references-count":52,"alternative-id":["10.3389\/fcimb.2020.00029"],"URL":"https:\/\/doi.org\/10.3389\/fcimb.2020.00029","relation":{},"ISSN":["2235-2988"],"issn-type":[{"type":"electronic","value":"2235-2988"}],"subject":[],"published":{"date-parts":[[2020,2,4]]},"article-number":"29"}}