{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T16:00:20Z","timestamp":1792166420273,"version":"4.4.0"},"reference-count":85,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2020,8,20]],"date-time":"2020-08-20T00:00:00Z","timestamp":1597881600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Cell. Infect. Microbiol."],"DOI":"10.3389\/fcimb.2020.00428","type":"journal-article","created":{"date-parts":[[2020,8,20]],"date-time":"2020-08-20T04:24:20Z","timestamp":1597897460000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":18,"title":["Legionella pneumophila Infection Rewires the Acanthamoeba castellanii Transcriptome, Highlighting a Class of Sirtuin Genes"],"prefix":"10.3389","volume":"10","author":[{"given":"Pengfei","family":"Li","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dane","family":"Vassiliadis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sze Ying","family":"Ong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vicki","family":"Bennett-Wood","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chihiro","family":"Sugimoto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junya","family":"Yamagishi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elizabeth L.","family":"Hartland","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shivani","family":"Pasricha","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2020,8,20]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"166","DOI":"10.1093\/bioinformatics\/btu638","article-title":"HTSeq\u2013a Python framework to work with high-throughput sequencing data","volume":"31","author":"Anders","year":"2015","journal-title":"Bioinformatics"},{"key":"B2","doi-asserted-by":"publisher","first-page":"283","DOI":"10.1046\/j.1462-2920.1999.00046.x","article-title":"Legionella: from environmental habitats to disease pathology, detection and control","volume":"1","author":"Atlas","year":"1999","journal-title":"Environ. Microbiol."},{"key":"B3","doi-asserted-by":"publisher","first-page":"2684","DOI":"10.1128\/AAC.39.12.2684","article-title":"Intraphagocytic growth induces an antibiotic-resistant phenotype of Legionella pneumophila","volume":"39","author":"Barker","year":"1995","journal-title":"Antimicrob. Agents Chemother"},{"key":"B4","doi-asserted-by":"publisher","first-page":"482","DOI":"10.3389\/fcimb.2017.00482","article-title":"Formation of the Legionella replicative compartment at the crossroads of retrograde trafficking","volume":"7","author":"Barlocher","year":"2017","journal-title":"Front. Cell Infect. Microbiol."},{"key":"B5","doi-asserted-by":"publisher","first-page":"279","DOI":"10.1128\/AEM.64.1.279-286.1998","article-title":"Production of respirable vesicles containing live Legionella pneumophila cells by two Acanthamoeba spp","volume":"64","author":"Berk","year":"1998","journal-title":"Appl. Environ. Microbiol."},{"key":"B6","doi-asserted-by":"publisher","first-page":"e01313","DOI":"10.1128\/mBio.01313-18","article-title":"Evolution of the Arsenal of Legionella pneumophila Effectors To Modulate Protist Hosts","volume":"9","author":"Best","year":"2018","journal-title":"mBio"},{"key":"B7","doi-asserted-by":"publisher","first-page":"3710","DOI":"10.1093\/bioinformatics\/bth456","article-title":"GO::TermFinder\u2013open source software for accessing Gene Ontology information and finding significantly enriched Gene Ontology terms associated with a list of genes","volume":"20","author":"Boyle","year":"2004","journal-title":"Bioinformatics"},{"key":"B8","doi-asserted-by":"publisher","first-page":"942","DOI":"10.2174\/138945011795677782","article-title":"The professional phagocyte Dictyostelium discoideum as a model host for bacterial pathogens","volume":"12","author":"Bozzaro","year":"2011","journal-title":"Curr. Drug Targets"},{"key":"B9","doi-asserted-by":"publisher","first-page":"20160047","DOI":"10.1098\/rsfs.2016.0047","article-title":"Progress and perspectives in signal transduction, actin dynamics, and movement at the cell and tissue level: lessons from Dictyostelium","volume":"6","author":"Bretschneider","year":"2016","journal-title":"Interface Focus"},{"key":"B10","doi-asserted-by":"publisher","first-page":"118","DOI":"10.1002\/mbo3.316","article-title":"Identification of regions within the Legionella pneumophila VipA effector protein involved in actin binding and polymerization and in interference with eukaryotic organelle trafficking","volume":"5","author":"Bugalhao","year":"2016","journal-title":"Microbiologyopen"},{"key":"B11","doi-asserted-by":"publisher","first-page":"e1000508","DOI":"10.1371\/journal.ppat.1000508","article-title":"Genome-scale identification of Legionella pneumophila effectors using a machine learning approach","volume":"5","author":"Burstein","year":"2009","journal-title":"PLoS Pathog"},{"key":"B12","doi-asserted-by":"publisher","first-page":"283","DOI":"10.1016\/S0074-7696(08)62000-8","article-title":"Growth, reproduction, and differentiation in Acanthamoeba","volume":"61","author":"Byers","year":"1979","journal-title":"Int. Rev. Cytol."},{"key":"B13","doi-asserted-by":"publisher","first-page":"1125","DOI":"10.1007\/s00436-012-3261-7","article-title":"Acanthamoeba castellanii cysts: new ultrastructural findings","volume":"112","author":"Ch\u00e1vez-Mungu\u00eda","year":"2013","journal-title":"Parasitol. Res."},{"key":"B14","doi-asserted-by":"publisher","first-page":"3254","DOI":"10.1128\/IAI.62.8.3254-3261.1994","article-title":"Growth of Legionella pneumophila in Acanthamoeba castellanii enhances invasion","volume":"62","author":"Cirillo","year":"1994","journal-title":"Infect. Immun."},{"key":"B15","doi-asserted-by":"publisher","first-page":"R11","DOI":"10.1186\/gb-2013-14-2-r11","article-title":"Genome of Acanthamoeba castellanii highlights extensive lateral gene transfer and early evolution of tyrosine kinase signaling","volume":"14","author":"Clarke","year":"2013","journal-title":"Genome Biol."},{"key":"B16","doi-asserted-by":"publisher","first-page":"214","DOI":"10.1016\/j.mimet.2007.11.009","article-title":"A faster and more accurate assay for intracellular replication of Legionella pneumophila in amoebae hosts","volume":"72","author":"Coil","year":"2008","journal-title":"J. Microbiol. Methods"},{"key":"B17","doi-asserted-by":"publisher","first-page":"619832","DOI":"10.1155\/2008\/619832","article-title":"Blast2GO: a comprehensive suite for functional analysis in plant genomics","volume":"2008","author":"Conesa","year":"2008","journal-title":"Int. J. Plant Genomics"},{"key":"B18","doi-asserted-by":"publisher","first-page":"3674","DOI":"10.1093\/bioinformatics\/bti610","article-title":"Blast2GO: a universal tool for annotation, visualization and analysis in functional genomics research","volume":"21","author":"Conesa","year":"2005","journal-title":"Bioinformatics"},{"key":"B19","doi-asserted-by":"publisher","first-page":"2689","DOI":"10.1128\/JCM.00309-10","article-title":"Resistance of Acanthamoeba cysts to disinfection treatments used in health care settings","volume":"48","author":"Coulon","year":"2010","journal-title":"J. Clin. Microbiol."},{"key":"B20","doi-asserted-by":"publisher","first-page":"e02345","DOI":"10.1128\/mBio.02345-16","article-title":"Host cell S phase restricts Legionella pneumophila intracellular replication by destabilizing the membrane-bound replication compartment","volume":"8","author":"de Jesus-Diaz","year":"2017","journal-title":"mBio"},{"key":"B21","doi-asserted-by":"publisher","first-page":"S385","DOI":"10.1093\/clind\/13.Supplement_5.S385","article-title":"Ecology of Acanthamoeba","volume":"13","author":"De Jonckheere","year":"1991","journal-title":"Rev. Infect. Dis."},{"key":"B22","doi-asserted-by":"publisher","first-page":"3048","DOI":"10.1128\/IAI.72.5.3048-3053.2004","article-title":"Legionella pneumophila replication vacuole formation involves rapid recruitment of proteins of the early secretory system","volume":"72","author":"Derre","year":"2004","journal-title":"Infect. Immun."},{"key":"B23","doi-asserted-by":"publisher","first-page":"67","DOI":"10.1016\/j.mib.2008.12.004","article-title":"Legionella pneumophila Dot\/Icm translocated substrates: a sum of parts","volume":"12","author":"Ensminger","year":"2009","journal-title":"Curr. Opin. Microbiol."},{"key":"B24","doi-asserted-by":"publisher","first-page":"302","DOI":"10.1016\/j.chom.2017.07.020","article-title":"Legionella pneumophila modulates mitochondrial dynamics to trigger metabolic repurposing of infected macrophages","volume":"22","author":"Escoll","year":"2017","journal-title":"Cell Host Microbe"},{"key":"B25","doi-asserted-by":"publisher","first-page":"438","DOI":"10.1111\/j.1462-5822.2005.00633.x","article-title":"Dictyostelium transcriptional host cell response upon infection with Legionella","volume":"8","author":"Farbrother","year":"2006","journal-title":"Cell Microbiol."},{"key":"B26","doi-asserted-by":"publisher","first-page":"506","DOI":"10.1128\/CMR.15.3.506-526.2002","article-title":"Legionella and Legionnaires' disease: 25 years of investigation","volume":"15","author":"Fields","year":"2002","journal-title":"Clin. Microbiol. Rev."},{"key":"B27","doi-asserted-by":"publisher","first-page":"e1002546","DOI":"10.1371\/journal.ppat.1002546","article-title":"The Legionella pneumophila effector VipA is an actin nucleator that alters host cell organelle trafficking","volume":"8","author":"Franco","year":"2012","journal-title":"PLoS Pathog."},{"key":"B28","doi-asserted-by":"publisher","first-page":"793","DOI":"10.1006\/bbrc.2000.3000","article-title":"Phylogenetic classification of prokaryotic and eukaryotic Sir2-like proteins","volume":"273","author":"Frye","year":"2000","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"B29","doi-asserted-by":"publisher","first-page":"576","DOI":"10.15585\/mmwr.mm6522e1","article-title":"Vital signs: deficiencies in environmental control identified in outbreaks of Legionnaires' Disease - North America, 2000-2014","volume":"65","author":"Garrison","year":"2016","journal-title":"Morb. Mortal. Wkly. Rep."},{"key":"B30","doi-asserted-by":"publisher","first-page":"919","DOI":"10.1093\/bioinformatics\/btr059","article-title":"B2G-FAR, a species-centered GO annotation repository","volume":"27","author":"Gotz","year":"2011","journal-title":"Bioinformatics"},{"key":"B31","doi-asserted-by":"publisher","first-page":"3420","DOI":"10.1093\/nar\/gkn176","article-title":"High-throughput functional annotation and data mining with the Blast2GO suite","volume":"36","author":"Gotz","year":"2008","journal-title":"Nucleic Acids Res."},{"key":"B32","doi-asserted-by":"publisher","first-page":"407","DOI":"10.1007\/s10059-009-0169-x","article-title":"Sirtuin\/Sir2 phylogeny, evolutionary considerations and structural conservation","volume":"28","author":"Greiss","year":"2009","journal-title":"Mol. Cells"},{"key":"B33","doi-asserted-by":"publisher","first-page":"413","DOI":"10.1128\/CMR.17.2.413-433.2004","article-title":"Microorganisms resistant to free-living amoebae","volume":"17","author":"Greub","year":"2004","journal-title":"Clin. Microbiol. Rev."},{"key":"B34","doi-asserted-by":"publisher","first-page":"225","DOI":"10.1016\/j.micinf.2013.11.007","article-title":"A Legionella effector modulates host cytoskeletal structure by inhibiting actin polymerization","volume":"16","author":"Guo","year":"2014","journal-title":"Microbes Infect."},{"key":"B35","doi-asserted-by":"publisher","first-page":"1","DOI":"10.4161\/sgtp.28651","article-title":"Beyond Rab GTPases Legionella activates the small GTPase Ran to promote microtubule polymerization, pathogen vacuole motility, and infection","volume":"5","author":"Hilbi","year":"2014","journal-title":"Small GTPases"},{"key":"B36","doi-asserted-by":"publisher","first-page":"79","DOI":"10.3389\/fmicb.2017.00079","article-title":"Intra-species and inter-kingdom signaling of Legionella pneumophila","volume":"8","author":"Hochstrasser","year":"2017","journal-title":"Front Microbiol"},{"key":"B37","doi-asserted-by":"publisher","first-page":"227","DOI":"10.1111\/j.1462-5822.2010.01531.x","article-title":"The E Block motif is associated with Legionella pneumophila translocated substrates","volume":"13","author":"Huang","year":"2011","journal-title":"Cell Microbiol."},{"key":"B38","doi-asserted-by":"publisher","first-page":"261","DOI":"10.1146\/annurev-cellbio-100109-104034","article-title":"Modulation of host cell function by Legionella pneumophila type IV effectors","volume":"26","author":"Hubber","year":"2010","journal-title":"Annu. Rev. Cell Dev. Biol."},{"key":"B39","doi-asserted-by":"publisher","first-page":"795","DOI":"10.1038\/35001622","article-title":"Transcriptional silencing and longevity protein Sir2 is an NAD-dependent histone deacetylase","volume":"403","author":"Imai","year":"2000","journal-title":"Nature"},{"key":"B40","doi-asserted-by":"publisher","first-page":"365","DOI":"10.1038\/nature06336","article-title":"Legionella pneumophila proteins that regulate Rab1 membrane cycling","volume":"450","author":"Ingmundson","year":"2007","journal-title":"Nature"},{"key":"B41","doi-asserted-by":"publisher","first-page":"275","DOI":"10.1128\/IAI.00703-13","article-title":"Human lung tissue explants reveal novel interactions during Legionella pneumophila infections","volume":"82","author":"Jager","year":"2014","journal-title":"Infect. Immun."},{"key":"B42","doi-asserted-by":"publisher","first-page":"945","DOI":"10.1038\/ncb883","article-title":"Legionella phagosomes intercept vesicular traffic from endoplasmic reticulum exit sites","volume":"4","author":"Kagan","year":"2002","journal-title":"Nat. Cell. Biol."},{"key":"B43","doi-asserted-by":"publisher","first-page":"1201","DOI":"10.1084\/jem.20031706","article-title":"Legionella subvert the functions of Rab1 and Sec22b to create a replicative organelle","volume":"199","author":"Kagan","year":"2004","journal-title":"J. Exp. Med."},{"key":"B44","doi-asserted-by":"publisher","first-page":"R36","DOI":"10.1186\/gb-2013-14-4-r36","article-title":"TopHat2: accurate alignment of transcriptomes in the presence of insertions, deletions and gene fusions","volume":"14","author":"Kim","year":"2013","journal-title":"Genome Biol"},{"key":"B45","doi-asserted-by":"publisher","first-page":"961","DOI":"10.1186\/s12864-019-6269-x","article-title":"Investigation of the host transcriptional response to intracellular bacterial infection using Dictyostelium discoideum as a host model","volume":"20","author":"Kjellin","year":"2019","journal-title":"BMC Genomics"},{"key":"B46","doi-asserted-by":"publisher","first-page":"1870","DOI":"10.1093\/molbev\/msw054","article-title":"MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets","volume":"33","author":"Kumar","year":"2016","journal-title":"Mol. Biol. Evol."},{"key":"B47","doi-asserted-by":"publisher","first-page":"e1006186","DOI":"10.1371\/journal.ppat.1006186","article-title":"A Legionella effector disrupts host cytoskeletal structure by cleaving actin","volume":"13","author":"Liu","year":"2017","journal-title":"PLoS Pathog."},{"key":"B48","doi-asserted-by":"publisher","first-page":"95","DOI":"10.1387\/ijdb.160038ss","article-title":"Dictyostelium discoideum Sir2D modulates cell-type specific gene expression and is involved in autophagy","volume":"61","author":"Lohia","year":"2017","journal-title":"Int. J. Dev. Biol."},{"key":"B49","doi-asserted-by":"publisher","first-page":"10","DOI":"10.1016\/j.molbiopara.2005.07.001","article-title":"RNA interference (RNAi) for the silencing of extracellular serine proteases genes in Acanthamoeba: molecular analysis and effect on pathogenecity","volume":"144","author":"Lorenzo-Morales","year":"2005","journal-title":"Mol. Biochem. Parasitol."},{"key":"B50","doi-asserted-by":"publisher","first-page":"550","DOI":"10.1186\/s13059-014-0550-8","article-title":"Moderated estimation of fold change and dispersion for RNA-seq data with DESeq2","volume":"15","author":"Love","year":"2014","journal-title":"Genome Biol."},{"key":"B51","doi-asserted-by":"publisher","first-page":"47","DOI":"10.1016\/j.devcel.2006.05.013","article-title":"Targeting of host Rab GTPase function by the intravacuolar pathogen Legionella pneumophila","volume":"11","author":"Machner","year":"2006","journal-title":"Dev. Cell."},{"key":"B52","doi-asserted-by":"publisher","first-page":"855","DOI":"10.1016\/j.pt.2016.08.006","article-title":"Asexual amoebae escape muller's ratchet through polyploidy","volume":"32","author":"Maciver","year":"2016","journal-title":"Trends Parasitol."},{"key":"B53","doi-asserted-by":"publisher","first-page":"323","DOI":"10.1016\/S1434-4610(98)70039-2","article-title":"Chromosome size polymorphisms in the genus Acanthamoeba electrokaryotype by pulsed-field gel electrophoresis","volume":"149","author":"Matsunaga","year":"1998","journal-title":"Protist"},{"key":"B54","doi-asserted-by":"publisher","first-page":"36448","DOI":"10.1038\/srep36448","article-title":"Legionella pneumophila prevents proliferation of its natural host Acanthamoeba castellanii","volume":"6","author":"Mengue","year":"2016","journal-title":"Sci. Rep."},{"key":"B55","doi-asserted-by":"publisher","first-page":"e00354","DOI":"10.1128\/mBio.00354-15","article-title":"The Legionella Kinase LegK2 Targets the ARP2\/3 complex to inhibit actin nucleation on phagosomes and allow bacterial evasion of the late endocytic pathway","volume":"6","author":"Michard","year":"2015","journal-title":"mBio"},{"key":"B56","doi-asserted-by":"publisher","first-page":"20","DOI":"10.1128\/AEM.71.1.20-28.2005","article-title":"Amoebae as training grounds for intracellular bacterial pathogens","volume":"71","author":"Molmeret","year":"2005","journal-title":"Appl. Environ. Microbiol."},{"key":"B57","doi-asserted-by":"publisher","first-page":"439","DOI":"10.1146\/annurev-pathmechdis-012419-032742","article-title":"Legionnaires' disease: state of the art knowledge of pathogenesis mechanisms of Legionella","volume":"15","author":"Mondino","year":"2020","journal-title":"Annu. Rev. Pathol."},{"key":"B58","doi-asserted-by":"publisher","first-page":"679","DOI":"10.1126\/science.1067025","article-title":"A bacterial guanine nucleotide exchange factor activates ARF on Legionella phagosomes","volume":"295","author":"Nagai","year":"2002","journal-title":"Science"},{"key":"B59","doi-asserted-by":"publisher","first-page":"833","DOI":"10.1242\/jcs.081067","article-title":"Sirtuins at a glance","volume":"124","author":"Nakagawa","year":"2011","journal-title":"J. Cell Sci."},{"key":"B60","doi-asserted-by":"publisher","first-page":"914","DOI":"10.1128\/AEM.66.3.914-919.2000","article-title":"Influence of Acanthamoeba castellanii on intracellular growth of different Legionella species in human monocytes","volume":"66","author":"Neumeister","year":"2000","journal-title":"Appl. Environ. Microbiol."},{"key":"B61","doi-asserted-by":"publisher","first-page":"453","DOI":"10.1126\/science.1207193","article-title":"De-AMPylation of the small GTPase Rab1 by the pathogen Legionella pneumophila","volume":"333","author":"Neunuebel","year":"2011","journal-title":"Science"},{"key":"B62","doi-asserted-by":"publisher","first-page":"28","DOI":"10.4161\/sgtp.18704","article-title":"The taming of a Rab GTPase by Legionella pneumophila","volume":"3","author":"Neunuebel","year":"2012","journal-title":"Small GTPases"},{"key":"B63","doi-asserted-by":"publisher","first-page":"1389","DOI":"10.1128\/JB.06306-11","article-title":"Legionella pneumophila LidA affects nucleotide binding and activity of the host GTPase Rab1","volume":"194","author":"Neunuebel","year":"2012","journal-title":"J. Bacteriol."},{"key":"B64","doi-asserted-by":"publisher","first-page":"274","DOI":"10.1128\/CMR.00052-09","article-title":"Molecular pathogenesis of infections caused by Legionella pneumophila","volume":"23","author":"Newton","year":"2010","journal-title":"Clin. Microbiol. Rev."},{"key":"B65","doi-asserted-by":"publisher","first-page":"e114914","DOI":"10.1371\/journal.pone.0114914","article-title":"The Transcriptome of Legionella pneumophila-infected human monocyte-derived macrophages","volume":"9","author":"Price","year":"2014","journal-title":"PLoS ONE"},{"key":"B66","doi-asserted-by":"publisher","first-page":"487","DOI":"10.3389\/fcimb.2017.00487","article-title":"Hijacking of the host ubiquitin network by Legionella pneumophila","volume":"7","author":"Qiu","year":"2017","journal-title":"Front. Cell. Infect. Microbiol."},{"key":"B67","doi-asserted-by":"publisher","first-page":"134","DOI":"10.1016\/S0076-6879(06)11009-5","article-title":"TM4 microarray software suite","volume":"411","author":"Saeed","year":"2006","journal-title":"Methods Enzymol."},{"key":"B68","doi-asserted-by":"publisher","first-page":"894","DOI":"10.1111\/j.1469-0691.2009.03011.x","article-title":"Free-living amoebae, a training field for macrophage resistance of mycobacteria","volume":"15","author":"Salah","year":"2009","journal-title":"Clin. Microbiol. Infect."},{"key":"B69","doi-asserted-by":"publisher","first-page":"622","DOI":"10.1074\/mcp.M116.063453","article-title":"Comparative proteomics of purified pathogen vacuoles correlates intracellular replication of Legionella pneumophila with the small GTPase RAS-related protein 1 (Rap1)","volume":"16","author":"Schm\u00f6lders","year":"2017","journal-title":"Mol. Cell. Proteomics"},{"key":"B70","doi-asserted-by":"publisher","first-page":"3221","DOI":"10.1073\/pnas.1820093116","article-title":"Legionella pneumophila translocated translation inhibitors are required for bacterial-induced host cell cycle arrest","volume":"116","author":"Sol","year":"2019","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"B71","doi-asserted-by":"publisher","first-page":"3245","DOI":"10.1128\/AEM.59.10.3245-3249.1993","article-title":"Stimulatory effect of cooling tower biocides on amoebae","volume":"59","author":"Srikanth","year":"1993","journal-title":"Appl. Environ. Microbiol."},{"key":"B72","doi-asserted-by":"publisher","first-page":"639","DOI":"10.1038\/nrm2447","article-title":"Shaping cups into phagosomes and macropinosomes","volume":"9","author":"Swanson","year":"2008","journal-title":"Nat. Rev. Mol. Cell. Biol."},{"key":"B73","doi-asserted-by":"publisher","first-page":"3609","DOI":"10.1128\/IAI.63.9.3609-3620.1995","article-title":"Association of Legionella pneumophila with the macrophage endoplasmic reticulum","volume":"63","author":"Swanson","year":"1995","journal-title":"Infection Immunity"},{"key":"B74","doi-asserted-by":"publisher","first-page":"61","DOI":"10.3389\/fcimb.2018.00061","article-title":"Acanthamoeba and Dictyostelium as cellular models for Legionella infection","volume":"8","author":"Swart","year":"2018","journal-title":"Front. Cell Infect. Microbiol."},{"key":"B75","doi-asserted-by":"publisher","first-page":"506","DOI":"10.1038\/nature10307","article-title":"Legionella pneumophila SidD is a deAMPylase that modifies Rab1","volume":"475","author":"Tan","year":"2011","journal-title":"Nature"},{"key":"B76","doi-asserted-by":"publisher","first-page":"3609","DOI":"10.1128\/IAI.00439-13","article-title":"Transcriptome analysis of mouse brain infected with Toxoplasma gondii","volume":"81","author":"Tanaka","year":"2013","journal-title":"Infection Immunity"},{"key":"B77","doi-asserted-by":"publisher","first-page":"538","DOI":"10.1007\/s00248-009-9514-z","article-title":"Legionella, protozoa, and biofilms: interactions within complex microbial systems","volume":"58","author":"Taylor","year":"2009","journal-title":"Microb. Ecol."},{"key":"B78","doi-asserted-by":"publisher","first-page":"27","DOI":"10.1093\/jac\/46.1.27","article-title":"Emergence of resistance to biocides during differentiation of Acanthamoeba castellanii","volume":"46","author":"Turner","year":"2000","journal-title":"J. Antimicrob. Chemother"},{"key":"B79","doi-asserted-by":"publisher","first-page":"11566","DOI":"10.1021\/es303212a","article-title":"Effect of disinfectant, water age, and pipe material on occurrence and persistence of Legionella, mycobacteria, Pseudomonas aeruginosa, and two amoebas","volume":"46","author":"Wang","year":"2012","journal-title":"Environ. Sci. Technol."},{"key":"B80","doi-asserted-by":"publisher","first-page":"e02420","DOI":"10.1128\/mBio.02420-18","article-title":"Legionella-containing vacuoles capture PtdIns(4)P-rich vesicles derived from the golgi apparatus","volume":"9","author":"Weber","year":"2018","journal-title":"mBio"},{"key":"B81","doi-asserted-by":"publisher","first-page":"e00839","DOI":"10.1128\/mBio.00839-13","article-title":"Live-cell imaging of phosphoinositide dynamics and membrane architecture during Legionella infection","volume":"5","author":"Weber","year":"2014","journal-title":"mBio"},{"key":"B82","doi-asserted-by":"publisher","first-page":"843","DOI":"10.1242\/jcs.01660","article-title":"The Ras superfamily at a glance","volume":"118","author":"Wennerberg","year":"2005","journal-title":"J. Cell Sci."},{"key":"B83","doi-asserted-by":"publisher","first-page":"383","DOI":"10.1080\/00365549950163833","article-title":"Free-living amoebae protecting Legionella in water: the tip of an iceberg?","volume":"31","author":"Winiecka-Krusnell","year":"1999","journal-title":"Scand J. Infect. Dis."},{"key":"B84","doi-asserted-by":"publisher","first-page":"17638","DOI":"10.1371\/journal.pone.0017638","article-title":"Comprehensive identification of protein substrates of the Dot\/Icm type IV transporter of Legionella pneumophila","author":"Zhu","year":"2011","journal-title":"PLoS ONE"},{"key":"B85","doi-asserted-by":"publisher","first-page":"97","DOI":"10.1016\/B978-1-4832-2734-4.50017-6","author":"Zuckerland","year":"1965","journal-title":"Evolutionary Divergence and Convergence in Proteins"}],"container-title":["Frontiers in Cellular and Infection Microbiology"],"original-title":[],"link":[{"URL":"https:\/\/www.frontiersin.org\/article\/10.3389\/fcimb.2020.00428\/full","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,8,20]],"date-time":"2020-08-20T04:24:24Z","timestamp":1597897464000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.frontiersin.org\/article\/10.3389\/fcimb.2020.00428\/full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,8,20]]},"references-count":85,"alternative-id":["10.3389\/fcimb.2020.00428"],"URL":"https:\/\/doi.org\/10.3389\/fcimb.2020.00428","relation":{},"ISSN":["2235-2988"],"issn-type":[{"value":"2235-2988","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,8,20]]},"article-number":"428"}}