{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,17]],"date-time":"2026-07-17T06:23:06Z","timestamp":1784269386239,"version":"3.55.0"},"reference-count":33,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2020,12,2]],"date-time":"2020-12-02T00:00:00Z","timestamp":1606867200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Cell. Infect. Microbiol."],"abstract":"<jats:p><jats:italic>Candida albicans<\/jats:italic> is commensal in human microbiota and is known to be the commonest opportunistic pathogen, having variable clinical outcomes that can lead to up to 60% mortality. Such wide clinical behaviour can be attributed to its phenotypical plasticity and high genetic diversity. This study characterised 10 Colombian clinical isolates which had already been identified as <jats:italic>C. albicans<\/jats:italic> by molecular tests; however, previous bioinformatics analysis of protein mass spectra and phenotypical characteristics has shown that this group of isolates has atypical behaviour, sharing characteristics of both <jats:italic>C.\u00a0africana<\/jats:italic> and <jats:italic>C. albicans<\/jats:italic>. This study was aimed at evaluating atypical isolates\u2019 pathogenic capability in the <jats:italic>Galleria mellonella<\/jats:italic> model; susceptibility profiles were determined and MLST was used for molecular characterisation. Cluster analysis, enabling unbiased bootstrap to classify the isolates and establish their cluster membership and e-BURST, was used for establishing clonal complexes (CC). Both approaches involved using representative MLST data from the 18 traditional <jats:italic>C.\u00a0albicans<\/jats:italic> clades, as well as <jats:italic>C. albicans<\/jats:italic>-associated and minor species. Ten atypical isolates were distributed as follows: 6\/10 (B71, B41, B60, R6, R41, and R282) were grouped into a statistically well-supported atypical cluster (AC) and constituted a differentiated CC 6; 2\/10 of the isolates were clearly grouped in clade 1 and were concurrent in CC 4 (B80, B44). Another 2\/10 atypical isolates were grouped in clade 10 and concurred in CC 7 (R425, R111); most atypical isolates were related to geographically distant isolates and some represented new ST. Isolates B41 and R41 in the AC had greater virulence. Isolate B44 was fluconazole-resistant and was grouped in clade 1. The atypical nature of the isolates studied here was demonstrated by the contrast between phenotypical traits (<jats:italic>C. africana<\/jats:italic>-like), molecular markers (<jats:italic>C. albicans<\/jats:italic>-like), virulence, and antifungal resistance, highlighting the widely described genetic plasticity for this genus. Our results showed that the atypical isolates forming well-differentiated groups belonged to <jats:italic>C. albicans<\/jats:italic>. Our findings could contribute towards developing molecular epidemiology approaches for managing hospital-acquired infection.<\/jats:p>","DOI":"10.3389\/fcimb.2020.571147","type":"journal-article","created":{"date-parts":[[2020,12,2]],"date-time":"2020-12-02T05:33:34Z","timestamp":1606887214000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":1,"title":["Robust, Comprehensive Molecular, and Phenotypical Characterisation of Atypical Candida albicans Clinical Isolates From Bogot\u00e1, Colombia"],"prefix":"10.3389","volume":"10","author":[{"given":"Giovanni","family":"Rodr\u00edguez-Leguizam\u00f3n","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andr\u00e9s","family":"Ceballos-Garz\u00f3n","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carlos F.","family":"Su\u00e1rez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manuel A.","family":"Patarroyo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claudia M.","family":"Parra-Giraldo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2020,12,2]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"671","DOI":"10.1128\/CMR.00043-16","article-title":"Investigating clinical issues by genotyping of medically important fungi: Why and how","volume":"30","author":"Alanio","year":"2017","journal-title":"Clin. 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