{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,25]],"date-time":"2026-01-25T01:34:52Z","timestamp":1769304892919,"version":"3.49.0"},"reference-count":29,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2020,11,20]],"date-time":"2020-11-20T00:00:00Z","timestamp":1605830400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Cell. Infect. Microbiol."],"abstract":"<jats:p><jats:italic>Stapylococcus aureus<\/jats:italic> is a common infectious agent in <jats:italic>e.g.<\/jats:italic> sepsis, associated with both high mortality rates and severe long-term effects. The cytolytic protein \u03b1-hemolysin has repeatedly been shown to enhance the virulence of <jats:italic>S. aureus<\/jats:italic>. Combined with an unhindered spread of multi drug-resistant strains, this has triggered research into novel anti virulence (<jats:italic>i.e.<\/jats:italic> anti \u03b1-hemolysin) drugs. Their functionality will depend on our ability to identify infections that might be alleviated by such. We therefore saw a need for detection methods that could identify individuals suffering from <jats:italic>S. aureus<\/jats:italic> infections where \u03b1-hemolysin was a major determinant. Molecular imprinted polymers were subsequently prepared on gold coated sensor chips. Used in combination with a surface plasmon resonance biosensor, \u03b1-hemolysin could therethrough be quantified from septic blood samples (n = 9), without pre-culturing of the infectious agent. The biosensor recognized \u03b1-hemolysin with high affinity (K<jats:sub>D<\/jats:sub> = 2.75 x 10<jats:sup>-7<\/jats:sup> M) and demonstrated a statistically significant difference (<jats:italic>p<\/jats:italic> &amp;lt; 0.0001) between the \u03b1-hemolysin response and potential sample contaminants. The detection scheme proved equally good, or better, when compared to antibody-based detection methods. This novel detection scheme constitutes a more rapid, economical, and user-friendly alternative to many methods currently in use. Heightening both reproducibility and sensitivity, molecular imprinting in combination with surface plasmon resonance (SPR)-technology could be a versatile new tool in clinical- and research-settings alike.<\/jats:p>","DOI":"10.3389\/fcimb.2020.571578","type":"journal-article","created":{"date-parts":[[2020,11,20]],"date-time":"2020-11-20T06:29:43Z","timestamp":1605853783000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":24,"title":["Development of a Molecular Imprinting-Based Surface Plasmon Resonance Biosensor for Rapid and Sensitive Detection of Staphylococcus aureus Alpha Hemolysin From Human Serum"],"prefix":"10.3389","volume":"10","author":[{"given":"Tilde","family":"Andersson","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anna","family":"Bl\u00e4ckberg","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rolf","family":"Lood","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gizem","family":"Ert\u00fcrk Bergdahl","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1965","published-online":{"date-parts":[[2020,11,20]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"51","DOI":"10.1002\/jssc.201800945","article-title":"Advances in the development of molecularly imprinted polymers for the separation and analysis of proteins with liquid chromatography","volume":"42","author":"Boysen","year":"2019","journal-title":"J. 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