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Cell. Infect. Microbiol."],"abstract":"<jats:p>In a <jats:italic>Plasmodium vivax<\/jats:italic> infection, it was shown a proportionally increased on gametocyte distribution within the bone marrow aspirant, suggesting a role of this organ as a reservoir for this parasite stage. Here, we evaluated the <jats:italic>ex vivo<\/jats:italic> cytoadhesive capacity of <jats:italic>P. vivax<\/jats:italic> gametocytes to bone marrow endothelial cells (HBMEC) and investigated the involvement of some receptors in the cytoadhesion process by using transfected CHO cells (CHO-ICAM1, CHO-CD36 and CHO-VCAM), wild type (CHO-K1) or deficient in heparan and chondroitin sulfate (CHO-745). <jats:italic>Ex-vivo<\/jats:italic> cytoadhesion assays were performed using a total of 44\u00a0<jats:italic>P. vivax<\/jats:italic> isolates enriched in gametocyte stages by Percoll gradient in the different cell lines. The majority of isolates (88.9%) were able to adhere to HBMEC monolayer. ICAM1 seemed to be the sole receptor significantly involved. CD-36 was the receptor with higher adhesion rate, despite no significance was noticed when compared to CHO-745. We demonstrated that gametocyte <jats:italic>P. vivax<\/jats:italic> adheres <jats:italic>ex vivo<\/jats:italic> to bone marrow endothelial cells. Moreover, <jats:italic>P. vivax<\/jats:italic> gametocytes display the ability to adhere to all CHO cells investigated, especially to CHO-ICAM1. These findings bring insights to the comprehension of the role of the bone marrow as a <jats:italic>P. vivax<\/jats:italic> reservoir and the potential impact on parasite transmission to the vector.<\/jats:p>","DOI":"10.3389\/fcimb.2021.614985","type":"journal-article","created":{"date-parts":[[2021,6,24]],"date-time":"2021-06-24T12:15:37Z","timestamp":1624536937000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":13,"title":["Plasmodium vivax Gametocytes Adherence to Bone Marrow Endothelial Cells"],"prefix":"10.3389","volume":"11","author":[{"given":"Luis Carlos","family":"Salazar Alvarez","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Omaira","family":"Vera Lizcano","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dayanne Kamylla Alves","family":"da Silva Barros","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Djane Clarys","family":"Baia-da-Silva","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wuelton Marcelo","family":"Monteiro","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paulo Filemon Paolluci","family":"Pimenta","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcus Vinicius Guimar\u00e3es","family":"de Lacerda","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fabio Trindade Maranh\u00e3o","family":"Costa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefanie Costa Pinto","family":"Lopes","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2021,6,24]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"959","DOI":"10.1182\/blood-2013-08-520767","article-title":"Molecular Evidence for the Localization of Plasmodium Falciparum Immature Gametocytes in Bone Marrow","volume":"123","author":"Aguilar","year":"2014","journal-title":"Blood"},{"key":"B2","doi-asserted-by":"publisher","first-page":"e0005365","DOI":"10.1371\/journal.pntd.0005365","article-title":"Plasmodium Vivax Gametocytes in the Bone Marrow of an Acute Malaria Patient and Changes in the Erythroid miRNA Profile","volume":"11","author":"Baro","year":"2017","journal-title":"PloS Negl. 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