{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T04:12:03Z","timestamp":1787026323349,"version":"build-2736575974"},"reference-count":26,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2021,3,16]],"date-time":"2021-03-16T00:00:00Z","timestamp":1615852800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Cell. Infect. Microbiol."],"abstract":"<jats:p>\n                    The occurrence of multidrug-resistant\n                    <jats:italic>Candida auris<\/jats:italic>\n                    isolates and the increased mortality associated with invasive infections or outbreaks due to this\n                    <jats:italic>Candida<\/jats:italic>\n                    species have been reported in many healthcare settings. Therefore, accurate and rapid identification at the species level of clinical\n                    <jats:italic>C. auris<\/jats:italic>\n                    isolates as well as their timely differentiation as susceptible or resistant to antifungal drugs is mandatory. Aims of the present study were to implement the MALDI-TOF mass spectrometry (MS) Bruker Daltonics Biotyper\n                    <jats:sup>\u00ae<\/jats:sup>\n                    database with\n                    <jats:italic>C.\u00a0auris<\/jats:italic>\n                    spectrum profiles and to develop a fast and reproducible MS assay for detecting anidulafungin (AFG) resistance in\n                    <jats:italic>C. auris<\/jats:italic>\n                    isolates. After creation of main\n                    <jats:italic>C.\u00a0auris<\/jats:italic>\n                    spectra, a score-oriented dendrogram was generated from hierarchical cluster analysis, including spectra of isolates from\n                    <jats:italic>C. auris<\/jats:italic>\n                    and other\n                    <jats:italic>Candida<\/jats:italic>\n                    (\n                    <jats:italic>C. glabrata<\/jats:italic>\n                    ,\n                    <jats:italic>C.\u00a0guilliermondii<\/jats:italic>\n                    ,\n                    <jats:italic>C.\u00a0haemulonii<\/jats:italic>\n                    ,\n                    <jats:italic>C. lusitaniae<\/jats:italic>\n                    , and\n                    <jats:italic>C. parapsilosis<\/jats:italic>\n                    ) or non-\n                    <jats:italic>Candida<\/jats:italic>\n                    (\n                    <jats:italic>Rhodotorula glutinis<\/jats:italic>\n                    ) species. Cluster analysis allowed to group and classify the isolates according to their species designation. Then, a three-hour incubation antifungal susceptibility testing (AFST) assay was developed. Spectra obtained at null, intermediate, or maximum AFG concentrations were used to create composite correlation index matrices for eighteen\n                    <jats:italic>C. auris<\/jats:italic>\n                    isolates included in the study. All six resistant\n                    <jats:italic>C. auris<\/jats:italic>\n                    isolates were detected as resistant whereas 11 of 12\u00a0susceptible\n                    <jats:italic>C. auris<\/jats:italic>\n                    isolates were detected as susceptible by the MS-AFST assay. In\u00a0conclusion, our MS-based assay offers the possibility of rapidly diagnosing and appropriately treating patients with\n                    <jats:italic>C.\u00a0auris<\/jats:italic>\n                    infection.\n                  <\/jats:p>","DOI":"10.3389\/fcimb.2021.645049","type":"journal-article","created":{"date-parts":[[2021,3,16]],"date-time":"2021-03-16T02:12:20Z","timestamp":1615860740000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":17,"title":["Are We Ready for Nosocomial Candida auris Infections? Rapid Identification and Antifungal Resistance Detection Using MALDI-TOF Mass Spectrometry May Be the Answer"],"prefix":"10.3389","volume":"11","author":[{"given":"Elena","family":"De Carolis","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Federica","family":"Marchionni","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marilisa","family":"La Rosa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jacques F.","family":"Meis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anuradha","family":"Chowdhary","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brunella","family":"Posteraro","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maurizio","family":"Sanguinetti","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2021,3,16]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"e00485","DOI":"10.1128\/AAC.00485-17","article-title":"Comparison of EUCAST and CLSI reference microdilution MICs of eight antifungal compounds for Candida auris and associated tentative epidemiological cutoff values","volume":"61","author":"Arendrup","year":"2017","journal-title":"Antimicrob. 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