{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,20]],"date-time":"2026-02-20T21:37:54Z","timestamp":1771623474191,"version":"3.50.1"},"reference-count":23,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2021,3,19]],"date-time":"2021-03-19T00:00:00Z","timestamp":1616112000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Cell. Infect. Microbiol."],"abstract":"<jats:p>The bacteremia level as well as the administration of antibiotics before blood collection may significantly affect the recovery of bacterial pathogens from pediatric blood cultures in BacT\/Alert Virtuo or Bactec FX BC systems, which remain the common techniques to diagnose bacteremia in pediatric patients. We simulated pediatric blood cultures with low or intermediate bacteremia level to evaluate BacT\/Alert PF Plus and Bactec Peds Plus blood culture bottles for resin-based inactivation of 16 antibiotic\u2013bacterium combinations. Overall, 105\/192 (54.7%) of BacT\/Alert PF Plus bottles and 69\/192 (36.0%) of Bactec Peds Plus bottles allowed organisms to grow when exposed to antibiotics. In particular, both BacT\/Alert PF Plus and Bactec Peds Plus bottles proved to be effective with piperacillin\/tazobactam and <jats:italic>Pseudomonas aeruginosa<\/jats:italic> or with oxacillin and methicillin-susceptible <jats:italic>Staphylococcus aureus<\/jats:italic> (100% growth), whereas no effectiveness was apparent with ceftriaxone and <jats:italic>Escherichia coli<\/jats:italic>, <jats:italic>Streptococcus agalactiae<\/jats:italic>, or <jats:italic>Streptococcus pneumoniae<\/jats:italic> or with cefepime and <jats:italic>E. coli<\/jats:italic> (0% growth). In some relevant instances (<jats:italic>e.g.<\/jats:italic>, with vancomycin and methicillin-resistant <jats:italic>S. aureus<\/jats:italic> or <jats:italic>Streptococcus pneumoniae<\/jats:italic>), BacT\/Alert PF Plus bottles were superior to Bactec Peds Plus bottles. Together, these findings underscore the potentiality of resin-containing bottles to enhance diagnosis of bacteremia in pediatric patients on antimicrobial therapy. This is particularly true with one of the evaluated BC systems and with simulated intermediate bacteremia level only.<\/jats:p>","DOI":"10.3389\/fcimb.2021.649769","type":"journal-article","created":{"date-parts":[[2021,3,19]],"date-time":"2021-03-19T05:48:25Z","timestamp":1616132905000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":6,"title":["Simulated Pediatric Blood Cultures to Assess the Inactivation of Clinically Relevant Antimicrobial Drug Concentrations in Resin-Containing Bottles"],"prefix":"10.3389","volume":"11","author":[{"given":"Liliana","family":"Giordano","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Flora Marzia","family":"Liotti","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Giulia","family":"Menchinelli","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Giulia","family":"De Angelis","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tiziana","family":"D\u2019Inzeo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Grazia Angela","family":"Morandotti","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maurizio","family":"Sanguinetti","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Teresa","family":"Spanu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Brunella","family":"Posteraro","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1965","published-online":{"date-parts":[[2021,3,19]]},"reference":[{"key":"B1","first-page":"p 705","article-title":"Tables of antimicrobial agent pharmacology","volume-title":"Principles and practice of infectious diseases","author":"Amsden","year":"2010"},{"key":"B2","doi-asserted-by":"publisher","first-page":"75","DOI":"10.1016\/S0140-6736(18)30696-2","article-title":"Sepsis and septic shock","volume":"392","author":"Cecconi","year":"2018","journal-title":"Lancet"},{"key":"B3","doi-asserted-by":"publisher","first-page":"e01344","DOI":"10.1128\/JCM.01344-19","article-title":"Effect of clinically meaningful antibiotic concentrations on recovery of Escherichia coli and Klebsiella pneumoniae isolates from anaerobic blood culture bottles with and without antibiotic binding resins","volume":"57","author":"Chen","year":"2019","journal-title":"J. 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