{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,8]],"date-time":"2026-01-08T05:58:36Z","timestamp":1767851916109,"version":"3.49.0"},"reference-count":10,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2021,7,16]],"date-time":"2021-07-16T00:00:00Z","timestamp":1626393600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Cell. Infect. Microbiol."],"abstract":"<jats:sec><jats:title>Objectives<\/jats:title><jats:p>To evaluate the analytical and clinical performance of a prototype of a VIDAS<jats:sup>\u00ae<\/jats:sup> Galactomannan (GM) unitary test (bioM\u00e9rieux, Marcy l\u2019Etoile, France) and compare to that of the Platelia\u2122 <jats:italic>Aspergillus<\/jats:italic> Ag assay (Bio-Rad, CA, USA).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Repeatability, reproducibility, and freeze-thaw stability of VIDAS<jats:sup>\u00ae<\/jats:sup>GM were evaluated. Sera from patients at risk of IA were concurrently tested with both the VIDAS<jats:sup>\u00ae<\/jats:sup>GM and Platelia\u2122 <jats:italic>Aspergillus<\/jats:italic> Ag assays. Correlations between the two assays were assessed by Passing Bablok (PB) regression and performance by ROC analysis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The correlations between the VIDAS<jats:sup>\u00ae<\/jats:sup>GM indexes after one and two cycles of freezing\/thawing were r=1.00 and r=0.989, respectively. The coefficients of variation for negative, low-positive, and positive sera were 13%, 6%, and 5% for repeatability and 14.4%, 7.2%, and 5.5% for reproducibility. Overall, 126 sera were tested with both assays (44 fresh and 82 frozen). The correlation between VIDAS<jats:sup>\u00ae<\/jats:sup>GM and Platelia\u2122 <jats:italic>Aspergillus<\/jats:italic> Ag was r=0.798. The areas under the curve of the ROC analyses were 0.892 and 0.894, for VIDAS<jats:sup>\u00ae<\/jats:sup>GM and Platelia\u2122 <jats:italic>Aspergillus<\/jats:italic> Ag, respectively.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>This new VIDAS<jats:sup>\u00ae<\/jats:sup>GM prototype assay showed adequate analytical and clinical performance and a good correlation with that of Platelia\u2122 <jats:italic>Aspergillus<\/jats:italic> Ag with 126 sera, although these results need to be confirmed in a larger prospective and multicentric study. As for the other VIDAS<jats:sup>\u00ae<\/jats:sup> assays, VIDAS<jats:sup>\u00ae<\/jats:sup>GM is a single-sample automated test using a solid reagent strip and receptacle. It is easy to use and suitable for rapid on-demand test results.<\/jats:p><\/jats:sec>","DOI":"10.3389\/fcimb.2021.669237","type":"journal-article","created":{"date-parts":[[2021,7,16]],"date-time":"2021-07-16T09:22:10Z","timestamp":1626427330000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":5,"title":["Evaluation of a Prototype of a Novel Galactomannan Sandwich Assay Using the VIDAS\u00ae Technology for the Diagnosis of Invasive Aspergillosis"],"prefix":"10.3389","volume":"11","author":[{"given":"Salom\u00e9","family":"Gallet","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C\u00e9cile","family":"Garnaud","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C\u00e9line","family":"Dragonetti","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sophie","family":"Rivoiron","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Odile","family":"Cognet","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuping","family":"Guo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Myl\u00e8ne","family":"Les\u00e9n\u00e9chal","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dani\u00e8le","family":"Maubon","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Muriel","family":"Cornet","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1965","published-online":{"date-parts":[[2021,7,16]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"420","DOI":"10.1111\/myc.12628","article-title":"Improving Outcome of Fungal Diseases - Guiding Experts and Patients Towards Excellence","volume":"60","author":"Cornely","year":"2017","journal-title":"Mycoses"},{"key":"B2","doi-asserted-by":"publisher","first-page":"1367","DOI":"10.1093\/cid\/ciz1008","article-title":"Revision and Update of the Consensus Definitions of Invasive Fungal Disease From the European Organization for Research and Treatment of Cancer and the Mycoses Study Group Education and Research Consortium","volume":"71","author":"Donnelly","year":"2020","journal-title":"Clin. 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