{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,9]],"date-time":"2026-06-09T06:08:41Z","timestamp":1780985321770,"version":"3.54.1"},"reference-count":32,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2021,8,6]],"date-time":"2021-08-06T00:00:00Z","timestamp":1628208000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Cell. Infect. Microbiol."],"abstract":"<jats:p>Viridans streptococci are a group of \u03b1-hemolytic streptococcal species. They are mainly commensals, most abundant in the mouth supporting oral health. But they also include important human pathogens such as <jats:italic>Streptococcus pneumoniae<\/jats:italic>. Identification and molecular typing of viridans group streptococci are challenging, especially for members of the salivarius group. In this study, we developed a single-locus molecular typing method that is able to differentiate among the highly phylogenetically related members of the salivarius group (<jats:italic>S. salivarius<\/jats:italic>, <jats:italic>S. vestibularis<\/jats:italic> and <jats:italic>S. thermophilus<\/jats:italic>) and might support differentiation in other groups as well. This typing approach is based on the amplification and sequence analysis of the housekeeping gene dephospho-coenzyme A kinase (<jats:italic>coaE<\/jats:italic>), a gene with unrecognized taxonomic potential to date. Here, we analysed <jats:italic>coaE<\/jats:italic> gene sequences of 154 publicly available genomes and of 30 salivarius group isolates of our own collection that together belong to 20 different gram-positive bacterial (sub) species. Our results revealed that the <jats:italic>coaE<\/jats:italic> phylogeny distinguished between streptococcal and non-streptococcal genomes and that <jats:italic>coaE<\/jats:italic> gene sequences were species-specific. In contrast to MALDI-TOF MS performance, the <jats:italic>coaE<\/jats:italic> typing was able to precisely identify the phylogenetically very closely related members of the salivarius group.<\/jats:p>","DOI":"10.3389\/fcimb.2021.685657","type":"journal-article","created":{"date-parts":[[2021,8,6]],"date-time":"2021-08-06T07:10:27Z","timestamp":1628233827000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":6,"title":["A New Species-Specific Typing Method for Salivarius Group Streptococci Based on the Dephospho-Coenzyme A Kinase (coaE) Gene Sequencing"],"prefix":"10.3389","volume":"11","author":[{"given":"Mohamed M. 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