{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T14:37:34Z","timestamp":1780497454088,"version":"3.54.1"},"reference-count":63,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2021,6,24]],"date-time":"2021-06-24T00:00:00Z","timestamp":1624492800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000024","name":"Canadian Institutes of Health Research","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000024","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000245","name":"Michael Smith Foundation for Health Research","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000245","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Cell. Infect. Microbiol."],"abstract":"<jats:p>\n                    <jats:italic>Pseudomonas aeruginosa<\/jats:italic>\n                    is a metabolically versatile opportunistic pathogen capable of infecting distinct niches of the human body, including skin wounds and the lungs of cystic fibrosis patients. Eradication of\n                    <jats:italic>P. aeruginosa<\/jats:italic>\n                    infection is becoming increasingly difficult due to the numerous resistance mechanisms it employs. Adaptive resistance is characterized by a transient state of decreased susceptibility to antibiotic therapy that is distinct from acquired or intrinsic resistance, can be triggered by various environmental stimuli and reverted by removal of the stimulus. Further, adaptive resistance is intrinsically linked to lifestyles such as swarming motility and biofilm formation, both of which are important in infections and lead to multi-drug adaptive resistance. Here, we demonstrated that NtrBC, the master of nitrogen control, had a selective role in host colonization and a substantial role in determining intrinsic resistance to ciprofloxacin.\n                    <jats:italic>P. aeruginosa<\/jats:italic>\n                    mutant strains (\u0394\n                    <jats:italic>ntrB<\/jats:italic>\n                    , \u0394\n                    <jats:italic>ntrC<\/jats:italic>\n                    and \u0394\n                    <jats:italic>ntrBC<\/jats:italic>\n                    ) colonized the skin but not the respiratory tract of mice as well as WT and, unlike WT, could be reduced or eradicated from the skin by ciprofloxacin. We hypothesized that nutrient availability contributed to these phenomena and found that susceptibility to ciprofloxacin was impacted by nitrogen source in laboratory media.\n                    <jats:italic>P. aeruginosa<\/jats:italic>\n                    \u0394\n                    <jats:italic>ntrB<\/jats:italic>\n                    , \u0394\n                    <jats:italic>ntrC<\/jats:italic>\n                    and \u0394\n                    <jats:italic>ntrBC<\/jats:italic>\n                    also exhibited distinct host interactions, including modestly increased cytotoxicity toward human bronchial epithelial cells, reduced virulence factor production and 10-fold increased uptake by macrophages. These data might explain why NtrBC mutants were less adept at colonizing the upper respiratory tract of mice. Thus, NtrBC represents a link between nitrogen metabolism, adaptation and virulence of the pathogen\n                    <jats:italic>P. aeruginosa<\/jats:italic>\n                    , and could represent a target for eradication of recalcitrant infections\n                    <jats:italic>in situ<\/jats:italic>\n                    .\n                  <\/jats:p>","DOI":"10.3389\/fcimb.2021.694789","type":"journal-article","created":{"date-parts":[[2021,6,24]],"date-time":"2021-06-24T04:15:07Z","timestamp":1624508107000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":10,"title":["NtrBC Selectively Regulates Host-Pathogen Interactions, Virulence, and Ciprofloxacin Susceptibility of Pseudomonas aeruginosa"],"prefix":"10.3389","volume":"11","author":[{"given":"Morgan A.","family":"Alford","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Beverlie","family":"Baquir","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andy","family":"An","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ka-Yee G.","family":"Choi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert E. W.","family":"Hancock","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2021,6,24]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2020.00773","article-title":"NtrBC Regulates Invasiveness and Virulence of Pseudomonas aeruginosa During High-Density Infection","volume":"11","author":"Alford","year":"2020","journal-title":"Front. Microbiol."},{"key":"B2","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2021.621081","article-title":"Model of Sinusitis Infection for Screening Antimicrobial and Immunomodulatory Therapies","volume":"11","author":"Alford","year":"2021","journal-title":"Front. Cell. Infect. Microbiol."},{"key":"B3","doi-asserted-by":"publisher","first-page":"409","DOI":"10.1111\/1751-7915.13388","article-title":"Dismantling the Bacterial Virulence\u00a0Program","volume":"12","author":"Alford","year":"2019","journal-title":"Microb. Biotechnol."},{"key":"B4","doi-asserted-by":"publisher","first-page":"S113","DOI":"10.1086\/428051","article-title":"The Quinolones: Past, Present, and Future","volume":"41","author":"Andriole","year":"2005","journal-title":"Clin. Infect. Dis."},{"key":"B5","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms14631","article-title":"Reconstruction of the Metabolic Network of Pseudomonas aeruginosa to Interrogate Virulence Factor Synthesis","volume":"8","author":"Bartell","year":"2017","journal-title":"Nat. Commun."},{"key":"B6","doi-asserted-by":"publisher","first-page":"320","DOI":"10.1038\/nrmicro.2016.34","article-title":"Distinguishing Between Resistance, Tolerance and Persistence to Antibiotic Treatment","volume":"14","author":"Brauner","year":"2016","journal-title":"Nat. Rev. Microbiol."},{"key":"B7","doi-asserted-by":"publisher","first-page":"4276","DOI":"10.1128\/AAC.00830-07","article-title":"Role of Lon, An ATP-Dependent Protease Homolog, in Resistace of Pseudomonas Aeruginosa to Ciprofloxacin","volume":"51","author":"Brazas","year":"2007","journal-title":"Antimicrob. Agents Chemother."},{"key":"B8","doi-asserted-by":"publisher","first-page":"419","DOI":"10.1016\/j.tim.2011.04.005","article-title":"Pseudomonas Aeruginosa: All Roads Lead to Resistance","volume":"19","author":"Breidenstein","year":"2011","journal-title":"Trends Microbiol."},{"key":"B9","doi-asserted-by":"publisher","first-page":"4486","DOI":"10.1128\/AAC.00222-08","article-title":"Complex Ciprofloxacin Resistome Revealed by Screening a Pseudomonas Aeruginosa Mutant Library for Altered Susceptibility","volume":"52","author":"Breidenstein","year":"2008","journal-title":"Antimicrob. Agents Chemother."},{"key":"B10","doi-asserted-by":"publisher","first-page":"4115","DOI":"10.1038\/ncomms5115","article-title":"Nitrogen Stress Response and Stringent Response are Coupled in Escherichia Coli","volume":"5","author":"Brown","year":"2014","journal-title":"Nat. Commun."},{"key":"B11","doi-asserted-by":"publisher","first-page":"741","DOI":"10.1007\/978-1-4939-0473-0_57","article-title":"Assessing Pseudomonas Virulence Using Host Cells","volume":"1149","author":"Bucior","year":"2014","journal-title":"Methods Mol. Biol."},{"key":"B12","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb\/2019.00913","article-title":"Tolerance and Resistance of Pseudomonas aeruginosa Biofilms to Antimicrobial Agents - How P. Aeruginosa Can Escape Antibiotics","volume":"10","author":"Ciofu","year":"2019","journal-title":"Front. Microbiol."},{"key":"B13","volume-title":"Performance Standards for Antimicrobial Susceptibility Testing","year":"2020"},{"key":"B14","doi-asserted-by":"publisher","first-page":"e01999","DOI":"10.1128\/AAC.01999-19","article-title":"Multi-Drug Adaptive Resistance of Pseudomonas aeruginosa Swarming Cells","volume":"64","author":"Coleman","year":"2020","journal-title":"Antimicrob. Agents Chemother."},{"key":"B15","doi-asserted-by":"publisher","first-page":"jiaa778","DOI":"10.1093\/infdis\/jiaa778","article-title":"Contribution of Swarming Motility to Dissemination in a Pseudomonas aeruginosa Murine Skin Abscess Infection Model","author":"Coleman","year":"2020","journal-title":"J. Infect. Dis."},{"key":"B16","doi-asserted-by":"publisher","first-page":"2522","DOI":"10.1128\/AAC.00035-09","article-title":"Genomewide Identification of Genetic Determinants of Antimicrobial Drug Resistance in Pseudomonas aeruginosa","volume":"53","author":"D\u00f6tsch","year":"2009","journal-title":"Antimicrob. Agents Chemother."},{"key":"B17","doi-asserted-by":"publisher","first-page":"884","DOI":"10.1128\/jb.172.2.884-900.1990","article-title":"Identification and Characterization of Genes for a Second Anthranilate Synthase in Pseudomonas aeruginosa: Interchangeability of the Two Anthranilate Synthases and Evolutionary Implications","volume":"172","author":"Essar","year":"1990","journal-title":"J. Bacteriol."},{"key":"B18","doi-asserted-by":"publisher","first-page":"e1619","DOI":"10.1371\/journal.pone.0001619","article-title":"The Neglected Instrinsic Resistome of Bacterial Pathogens","volume":"3","author":"Fajardo","year":"2008","journal-title":"PLoS One"},{"key":"B19","doi-asserted-by":"publisher","first-page":"e55744","DOI":"10.7554\/eLife.55744","article-title":"Host-Induced Spermidine Production in Motile Pseudomonas aeruginosa Triggers Phagocytic Uptake","volume":"9","author":"Felgner","year":"2020","journal-title":"eLife"},{"key":"B20","doi-asserted-by":"publisher","first-page":"261","DOI":"10.2147\/idr.s182272","article-title":"Mutations in gyrB Play an Important Role in Ciprofloxacin-Resistant Pseudomonas aeruginosa","volume":"12","author":"Feng","year":"2019","journal-title":"Infect. Drug Resist."},{"key":"B21","doi-asserted-by":"publisher","first-page":"1128","DOI":"10.1128\/AAC.05336-11","article-title":"Role of Intracellular Proteases in the Antibiotic Resistance, Motility and Biofilm Formation of Pseudomonas aeruginosa","volume":"56","author":"Fern\u00e1ndez","year":"2012","journal-title":"Antimicrob. Agents Chemother."},{"key":"B22","doi-asserted-by":"publisher","first-page":"e0178178","DOI":"10.1371\/journal.pone.0178178","article-title":"Pseudomonas aeruginosa Urinary Tract Infections in Hospitalized Patients: Mortality and Prognostic Factors","volume":"12","author":"Ferreiro","year":"2017","journal-title":"PLoS One"},{"key":"B23","doi-asserted-by":"publisher","first-page":"563","DOI":"10.1038\/nrmicro.2016.94","article-title":"Biofilms: An Emergent Form of Bacterial Life","volume":"14","author":"Flemming","year":"2016","journal-title":"Nat. Rev. Microbiol."},{"key":"B24","doi-asserted-by":"publisher","first-page":"4780","DOI":"10.1038\/ncomms5780","article-title":"Pseudomonas aeruginosa Adaptation in the Nasopharyngeal Reservoir Leads to Migration and Persistence in the Lungs","volume":"5","author":"Fothergill","year":"2014","journal-title":"Nat. Commun."},{"key":"B25","doi-asserted-by":"publisher","first-page":"1734","DOI":"10.1111\/j.1462-2920.2010.02253.x","article-title":"How Pseudomonas aeruginosa Adapts to Various Environments: A Metabolomic Approach","volume":"12","author":"Frimmersdorf","year":"2010","journal-title":"Environ. Microbiol."},{"key":"B26","doi-asserted-by":"publisher","first-page":"580","DOI":"10.1016\/j.mib.2013.06.013","article-title":"Bacterial Biofilm Development as a Multicellular Adaptation: Antibiotic Resistance and New Therapeutic Strategies","volume":"16","author":"Fuente-Nunez","year":"2013","journal-title":"Curr. Opin. Microbiol."},{"key":"B27","doi-asserted-by":"publisher","first-page":"244","DOI":"10.1038\/nrmicro2537","article-title":"The Skin Microbiome","volume":"9","author":"Grice","year":"2011","journal-title":"Nat. Rev. Microbiol."},{"key":"B28","doi-asserted-by":"publisher","first-page":"e01313","DOI":"10.1128\/AAC.01313-19","article-title":"Potentiation of Aminoglycoside Lethality by C4-dicarboxylates Requires RpoN in Antibiotic-Tolerant Pseudomonas aeruginosa","volume":"63","author":"Hall","year":"2019","journal-title":"Antimicrob. Agents Chemother."},{"key":"B29","doi-asserted-by":"publisher","first-page":"654","DOI":"10.1038\/nrmicro2199","article-title":"The Type III Secretion System of Pseudomonas aeruginosa: Infection by Injection","volume":"7","author":"Hauser","year":"2009","journal-title":"Nat. Rev. Microbiol."},{"key":"B30","doi-asserted-by":"publisher","first-page":"975","DOI":"10.1016\/S2213-2600(20)30331-3","article-title":"Intravenous Versus Oral Antibiotics for Eradication of Pseudomonas aeruginosa in Cystic Fibrosis (TORPEDO-CF): A Randomised Controlled Trial","volume":"8","author":"Hewer","year":"2020","journal-title":"Lancet Respir. Med."},{"key":"B31","doi-asserted-by":"publisher","first-page":"20440","DOI":"10.1073\/pnas.0908760106","article-title":"Molecular Basis of Pyoverdine Siderophore Recycling in Pseudomonas aeruginosa","volume":"106","author":"Imperi","year":"2009","journal-title":"Proc. Natl. Acad. Sci. U.\u00a0S. A."},{"key":"B32","doi-asserted-by":"publisher","first-page":"3841","DOI":"10.1128\/IAI.57.12.3841-3845.1989","article-title":"Pseudomonas aeruginosa Pili as Ligands for Nonopsonic Phagocytosis by Fibronectin-Stimulated Macrophages","volume":"57","author":"Kelly","year":"1989","journal-title":"Infect. Immun."},{"key":"B33","doi-asserted-by":"publisher","first-page":"305","DOI":"10.1016\/j.isci.2019.02.028","article-title":"Context-Specific Requirement of Forty-Four Two-Component Loci in Pseudomonas aeruginosa Swarming","volume":"13","author":"Kollaran","year":"2019","journal-title":"iScience"},{"key":"B34","doi-asserted-by":"publisher","first-page":"657","DOI":"10.1139\/w09-024","article-title":"Induction of Pseudomonas aeruginosa Fhp and fhpR by Reactive Oxygen Species","volume":"55","author":"Koskenkorva-Frank","year":"2009","journal-title":"Can. J. Microbiol."},{"key":"B35","doi-asserted-by":"publisher","DOI":"10.1101\/pdb.prot095497","article-title":"Analysis of Cell Viability by the Lactate Dehydrogenase Assay","volume":"6","author":"Kumar","year":"2018","journal-title":"Cold Spring Harb. Protoc."},{"key":"B36","doi-asserted-by":"publisher","first-page":"126","DOI":"10.1111\/j.1462-2920.2008.01747.x","article-title":"Swarming Motility: A Multicellular Behaviour Conferring Antimicrobial Resistance","volume":"11","author":"Lai","year":"2009","journal-title":"Environ. Microbiol."},{"key":"B37","doi-asserted-by":"publisher","first-page":"78","DOI":"10.1093\/jac\/dkm129","article-title":"Evolution of Antimicrobial Resistance Among Pseudomonas aeruginosa, Acinetobacter baumannii and Klebsiella pneumoniae in Brooklyn, NY","volume":"60","author":"Landman","year":"2007","journal-title":"J. Antimicrob. Chemother."},{"key":"B38","doi-asserted-by":"publisher","DOI":"10.3390\/metabo9100234","article-title":"Adapting to the Airways: Metabolic Requirements of Pseudomonas aeruginosa During the Infection of Cystic Fibrosis Patients","volume":"9","author":"La Rosa","year":"2019","journal-title":"Metabolites"},{"key":"B39","doi-asserted-by":"publisher","first-page":"1232","DOI":"10.1099\/mic.0.000300","article-title":"Molecular Characterization of LhpR in Control of Hydroxyproline Catabolism and Transport in Pseudomonas aeruginosa PAO1","volume":"162","author":"Li","year":"2016","journal-title":"Microbiology (Read)"},{"key":"B40","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2014.00554","article-title":"Physiological Levels of Nitrate Support Anoxic Growth by Denitrification of Pseudomonas aeruginosa at Growth Rates Reported in Cystic Fibrosis Lungs and Sputum","volume":"5","author":"Line","year":"2014","journal-title":"Front. Microbiol."},{"key":"B41","doi-asserted-by":"publisher","first-page":"L591","DOI":"10.1152\/ajplung.00335.2013","article-title":"Mechanisms of Phagocytosis and Host Clearance of Pseudomonas aeruginosa","volume":"306","author":"Lovewell","year":"2014","journal-title":"Am. J. Physiol. Lung Cell Mol. Physiol."},{"key":"B42","doi-asserted-by":"publisher","first-page":"1838","DOI":"10.1042\/BST20110688","article-title":"Bacterial Nitrate Assimilation: Gene Distribution and Regulation","volume":"39","author":"Luque-Almagro","year":"2011","journal-title":"Biochem. Soc. Trans."},{"key":"B43","doi-asserted-by":"publisher","first-page":"195","DOI":"10.1016\/j.chembiol.2016.12.015","article-title":"Carbon Sources Tune Antibiotic Susceptibility in Pseudomonas aeruginosa via Tricarboxylic Acid Cycle Control","volume":"24","author":"Meylan","year":"2017","journal-title":"Cell Chem. Biol."},{"key":"B44","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2017.00039","article-title":"Pseudomonas aeruginosa Lifestyle: A Paradigm for Adaptation, Survival and Persistence","volume":"7","author":"Moradali","year":"2017","journal-title":"Front. Cell. Infect. Microbiol."},{"key":"B45","doi-asserted-by":"publisher","first-page":"3204","DOI":"10.1093\/nar\/gkab091","article-title":"Role of a Local Transcription Factor in Governing Cellular Carbon\/Nitrogen Homeostasis in Pseudomonas fluorescens","volume":"49","author":"Naren","year":"2021","journal-title":"Nucleic Acids Res."},{"key":"B46","doi-asserted-by":"publisher","first-page":"30","DOI":"10.1186\/s12866-017-0941-6","article-title":"High Virulence Sub-Populations in Pseudomonas aeruginosa Long-Term Cystic Fibrosis Airway Infections","volume":"17","author":"O\u2019Brien","year":"2017","journal-title":"BMC Microbiol."},{"key":"B47","doi-asserted-by":"publisher","first-page":"836","DOI":"10.1128\/jb.142.3.836-842.1980","article-title":"Isolation and Characterization of Pseudomonas aeruginosa PAO Mutant That Produces Altered Elastase","volume":"142","author":"Ohman","year":"1980","journal-title":"J. Bacteriol."},{"key":"B48","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2019.02894","article-title":"Clinical Impact of Antibiotics for the Treatment of Pseudomonas aeruginosa Biofilm Infections","volume":"10","author":"Olivares","year":"2020","journal-title":"Front. Microbiol."},{"key":"B49","doi-asserted-by":"publisher","first-page":"8079","DOI":"10.1128\/JB.01138-07","article-title":"Nutritional Cues Control Pseudomonas aeruginosa Behavior in Cystic Fibrosis Sputum","author":"Palmer","year":"2007"},{"key":"B50","doi-asserted-by":"publisher","first-page":"5267","DOI":"10.1128\/JB.187.15.5267-5277.2005","article-title":"Cystic Fibrosis Sputum Supports Growth and Cues Key Aspects of Pseudomonas aeruginosa Physiology","volume":"187","author":"Palmer","year":"2005","journal-title":"J. Bacteriol."},{"key":"B51","doi-asserted-by":"publisher","first-page":"9505","DOI":"10.1038\/s41598-020-66194-4","article-title":"Pseudomonas aeruginosa Core Metabolism Exerts a Widespread Growth-Independent Control on Virulence","volume":"10","author":"Panayidou","year":"2020","journal-title":"Sci. Rep."},{"key":"B52","doi-asserted-by":"publisher","first-page":"177","DOI":"10.1016\/j.biotechadv.2018.11.013","article-title":"Antibiotic Resistance in Pseudomonas aeruginosa: Mechanisms and Alternative Therapeutic Strategies","volume":"37","author":"Pang","year":"2019","journal-title":"Biotechnol. Adv."},{"key":"B53","doi-asserted-by":"publisher","first-page":"e02730","DOI":"10.1128\/mBio.02730-18","article-title":"A Shift in Central Metabolism Accompanies Virulence Activation in Pseudomonas aeruginosa","volume":"11","author":"Perinbam","year":"2020","journal-title":"mBio"},{"key":"B54","doi-asserted-by":"publisher","first-page":"e00140-17","DOI":"10.1128\/mBio.00140-17","article-title":"New Mouse Model for Chronic Infections by Gram-Negative Bacteria Enabling the Study of Anti-Infective Efficacy and Host-Microbe Interactions","volume":"8","author":"Pletzer","year":"2017","journal-title":"mBio"},{"key":"B55","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1099\/jmm.0.000873","article-title":"Mechanisms of Ciprofloxacin Resistance in Pseudomonas aeruginosa: New Approaches to an Old Problem","volume":"68","author":"Rehman","year":"2019","journal-title":"J. Med. Microbiol."},{"key":"B56","doi-asserted-by":"publisher","first-page":"717","DOI":"10.1007\/s13213-011-0273-y","article-title":"Contribution of an Arsenal of Virulence Factors to Pathogenesis of Pseudomonas aeruginosa","volume":"61","author":"Strateva","year":"2011","journal-title":"Ann. Microbiol."},{"key":"B57","doi-asserted-by":"publisher","DOI":"10.1038\/s41522-019-0113-6","article-title":"Parallel Evolutionary Paths to Produce More Than One Pseudomonas aeruginosa Biofilm Phenotype","volume":"6","author":"Th\u00f6ming","year":"2020","journal-title":"NPJ Biofilms Microbiomes"},{"key":"B58","doi-asserted-by":"publisher","first-page":"e0203295","DOI":"10.1371\/journal.pone.0203295","article-title":"Combination Therapy for Treatment of Pseudomonas aeruginosa Bloodstream Infections","volume":"13","author":"Tschudin-Sutter","year":"2018","journal-title":"PLoS One"},{"key":"B59","doi-asserted-by":"publisher","first-page":"327","DOI":"10.1016\/j.tim.2016.01.008","article-title":"Pseudomonas aeruginosa Evolutionary Adaptation and Diversification in Cystic Fibrosis Chronic Lung Infections","volume":"24","author":"Winstanley","year":"2016","journal-title":"Trends Microbiol."},{"key":"B60","doi-asserted-by":"publisher","first-page":"2599","DOI":"10.1128\/JB.01680-06","article-title":"Regulatory Role of PopN and Its Interacting Partners in Type III Secretion of Pseudomonas aeruginosa","volume":"189","author":"Yang","year":"2007","journal-title":"J. Bacteriol."},{"key":"B61","doi-asserted-by":"publisher","first-page":"1256","DOI":"10.1128\/IAI.01527-13","article-title":"Requirement of the Pseudomonas aeruginosa CbrA Sensor Kinase for Full Virulence in a Murine Acute Lung Infection Model","volume":"82","author":"Yeung","year":"2014","journal-title":"Infect. Immun."},{"key":"B62","doi-asserted-by":"publisher","first-page":"5592","DOI":"10.1128\/JB.00157-09","article-title":"Swarming of Pseudomonas aeruginosa is Controlled by a Broad Spectrum of Transcriptional Regulators, Including MetR","volume":"191","author":"Yeung","year":"2009","journal-title":"J. Bacteriol."},{"key":"B63","doi-asserted-by":"publisher","first-page":"e64554","DOI":"10.1361\/journal.pone.0064554","article-title":"Elucidating the Pseudomonas aeruginosa Fatty Acid Degradation Pathway: Identification of Additional Fatty acyl-CoA Synthetase Homologues","volume":"8","author":"Zarzycki-Siek","year":"2013","journal-title":"PLoS One"}],"container-title":["Frontiers in Cellular and Infection Microbiology"],"original-title":[],"link":[{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fcimb.2021.694789\/full","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,6,24]],"date-time":"2021-06-24T04:15:11Z","timestamp":1624508111000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fcimb.2021.694789\/full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,6,24]]},"references-count":63,"alternative-id":["10.3389\/fcimb.2021.694789"],"URL":"https:\/\/doi.org\/10.3389\/fcimb.2021.694789","relation":{"has-review":[{"id-type":"doi","id":"10.3410\/f.740463405.793587246","asserted-by":"object"}]},"ISSN":["2235-2988"],"issn-type":[{"value":"2235-2988","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,6,24]]},"article-number":"694789"}}