{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,7]],"date-time":"2026-07-07T14:30:42Z","timestamp":1783434642634,"version":"3.54.6"},"reference-count":25,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2021,9,22]],"date-time":"2021-09-22T00:00:00Z","timestamp":1632268800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Cell. Infect. Microbiol."],"abstract":"<jats:p><jats:italic>Haemophilus influenzae<\/jats:italic>is a major human pathogenic bacterium, resulting in a series of diseases, such as pneumonia, bacteremia, meningitis. However, it is hard to diagnose<jats:italic>H<\/jats:italic>.<jats:italic>influenzae<\/jats:italic>quickly. In this study, the multiple cross displacement amplification (MCDA) and nanoparticle-based lateral flow biosensor (LFB) (MCDA-LFB) were combined to detect<jats:italic>H<\/jats:italic>.<jats:italic>influenzae<\/jats:italic>, which has been proven to be reliable, rapid, and not complicated. On the basis of<jats:italic>H<\/jats:italic>.<jats:italic>influenzae<\/jats:italic>outer membrane protein<jats:italic>P6<\/jats:italic>gene, 10 specific primers were designed. The best MCDA condition was 61\u00b0C for 1 h. The sensitivity of<jats:italic>H<\/jats:italic>.<jats:italic>influenzae<\/jats:italic>-MCD-LFB assay showed, in the pure cultures, the minimum concentration of genomic DNA templates was 100 fg. The specificity of<jats:italic>H<\/jats:italic>.<jats:italic>influenzae<\/jats:italic>-MCD-LFB assay showed only<jats:italic>H<\/jats:italic>.<jats:italic>influenzae<\/jats:italic>templates were detected, and no cross-reactivity was found in non-<jats:italic>H<\/jats:italic>.<jats:italic>influenzae<\/jats:italic>isolates and other<jats:italic>Haemophilus<\/jats:italic>species. In 56 sputum samples, with MCDA-LFB method and PCR detection, 21 samples were positive, which was in consistent with the traditional culture method. The accuracy of diagnosis of MCDA-LFB, in comparison with the traditional culture method and PCR detection, can reach 100%, indicating that the MCDA-LFB assay gains an advantage over the cultured-based method for target pathogen detection. In conclusion, the MCDA-LFB assay is suitable for the sensitive, rapid, and specific detection of<jats:italic>H<\/jats:italic>.<jats:italic>influenzae<\/jats:italic>, which might be used as a potential diagnostic tool for<jats:italic>H<\/jats:italic>.<jats:italic>influenzae<\/jats:italic>in basic and clinical laboratories.<\/jats:p>","DOI":"10.3389\/fcimb.2021.721547","type":"journal-article","created":{"date-parts":[[2021,9,23]],"date-time":"2021-09-23T03:30:14Z","timestamp":1632367814000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":13,"title":["A Rapid Detection of Haemophilus influenzae Using Multiple Cross Displacement Amplification Linked With Nanoparticle-Based Lateral Flow Biosensor"],"prefix":"10.3389","volume":"11","author":[{"given":"Qilong","family":"Cao","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shaoshuai","family":"Liang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lin","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun","family":"Cao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mengyang","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shengpeng","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaolong","family":"Cao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Guo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2021,9,22]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"176","DOI":"10.3201\/eid0203.960303","article-title":"Conjugate Vaccines and the Carriage of Haemophilus Influenzae Type B","volume":"2","author":"Barbour","year":"1996","journal-title":"Emerg. 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