{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T01:27:29Z","timestamp":1780450049910,"version":"3.54.1"},"reference-count":65,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2021,10,12]],"date-time":"2021-10-12T00:00:00Z","timestamp":1633996800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004587","name":"Instituto de Salud Carlos III","doi-asserted-by":"publisher","award":["PI16\/00174,  FI17\/00248"],"award-info":[{"award-number":["PI16\/00174,  FI17\/00248"]}],"id":[{"id":"10.13039\/501100004587","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Cell. Infect. Microbiol."],"abstract":"<jats:sec><jats:title>Introduction<\/jats:title><jats:p>Antibiotics are commonly prescribed to young children for treating bacterial infections such as invasive pneumococcal disease (IPD) caused by <jats:italic>Streptococcus pneumoniae<\/jats:italic>. Despite the obvious benefits of antibiotics, little is known about their possible side effects on children\u2019s nasopharyngeal microbiota. In other ecological niches, antibiotics have been described to perturb the balanced microbiota with short- and long-term effects on children\u2019s health. The present study aims to evaluate and compare the nasopharyngeal microbiota of children with IPD and different degree of antibiotic exposure.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We investigated differences in nasopharyngeal microbiota of two groups of children &amp;lt;18 years with IPD: children not exposed to antibiotics before sample collection (n=27) compared to children previously exposed (n=54). Epidemiological\/clinical data were collected from subjects, and microbiota was characterized by Illumina sequencing of V3-V4 amplicons of the 16S rRNA gene.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Main epidemiological\/clinical factors were similar across groups. Antibiotic-exposed patients were treated during a median of 4 days (IQR: 3\u20136) with at least one beta-lactam (100.0%). Higher bacterial richness and diversity were found in the group exposed to antibiotics. Different streptococcal amplicon sequence variants (ASVs) were differentially abundant across groups: antibiotic use was associated to lower relative abundances of <jats:italic>Streptococcus<\/jats:italic> ASV2 and <jats:italic>Streptococcus<\/jats:italic> ASV11 (phylogenetically close to <jats:italic>S. pneumoniae<\/jats:italic>), and higher relative abundances of <jats:italic>Streptococcus<\/jats:italic> ASV3 and <jats:italic>Streptococcus<\/jats:italic> ASV12 (phylogenetically close to viridans group streptococci). ASVs assigned to typical bacteria from the oral cavity, including <jats:italic>Veillonella<\/jats:italic>, <jats:italic>Alloprevotella<\/jats:italic>, <jats:italic>Porphyromonas<\/jats:italic>, <jats:italic>Granulicatella<\/jats:italic>, or <jats:italic>Capnocytophaga<\/jats:italic>, were associated to the antibiotic-exposed group. Common nosocomial genera such as <jats:italic>Staphylococcus<\/jats:italic>, <jats:italic>Acinetobacter<\/jats:italic>, and <jats:italic>Pseudomonas<\/jats:italic> were also enriched in the group exposed to antibiotics.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Our results point toward a reduction of <jats:italic>S. pneumoniae<\/jats:italic> abundance on the nasopharynx of children with IPD after antibiotic treatment and a short-term repopulation of this altered niche by oral and nosocomial bacteria. Future research studies will have to evaluate the clinical implications of these findings and if these populations would benefit from the probiotic\/prebiotic administration or even from the improvement on oral hygiene practices frequently neglected among hospitalized children.<\/jats:p><\/jats:sec>","DOI":"10.3389\/fcimb.2021.744727","type":"journal-article","created":{"date-parts":[[2021,10,13]],"date-time":"2021-10-13T17:44:05Z","timestamp":1634147045000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":12,"title":["Rapid Increase of Oral Bacteria in Nasopharyngeal Microbiota After Antibiotic Treatment in Children With Invasive Pneumococcal Disease"],"prefix":"10.3389","volume":"11","author":[{"given":"Desiree","family":"Henares","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muntsa","family":"Rocafort","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pedro","family":"Brotons","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mariona F.","family":"de Sevilla","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alex","family":"Mira","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cristian","family":"Launes","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raul","family":"Cabrera-Rubio","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carmen","family":"Mu\u00f1oz-Almagro","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2021,10,12]]},"reference":[{"key":"B2","doi-asserted-by":"publisher","first-page":"5721","DOI":"10.1128\/JCM.43.11.5721-5732.2005","article-title":"Defining the Normal Bacterial Flora of the Oral Cavity","volume":"43","author":"Aas","year":"2005","journal-title":"J. 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