{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T12:14:39Z","timestamp":1789647279557,"version":"4.0.0"},"reference-count":44,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2021,9,10]],"date-time":"2021-09-10T00:00:00Z","timestamp":1631232000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Cell. Infect. Microbiol."],"abstract":"<jats:p>Avian leukosis virus subgroup J (ALV-J) is an oncogenic retrovirus that causes immunosuppression and neoplastic diseases in poultry. Cytokine signal-transduction inhibitor molecule 3 (SOCS3) is an important negative regulator of the JAK2\/STAT3 signaling pathway and plays certain roles in ALV-J infection. It is of significance to confirm the roles of <jats:italic>SOCS3<\/jats:italic> in ALV-J infection and study how this gene affects ALV-J infection. In this study, we assessed the expression of the <jats:italic>SOCS3<\/jats:italic> gene <jats:italic>in vivo<\/jats:italic> and <jats:italic>in vitro<\/jats:italic>, and investigated the roles of <jats:italic>SOCS3<\/jats:italic> in ALV-J infection using overexpressed or interfered assays with the <jats:italic>SOCS3<\/jats:italic> in DF-1 cells. The results showed that the <jats:italic>SOCS3<\/jats:italic> expression of ALV-J infected chickens was different from uninfected chickens in the spleen, thymus and cecal tonsil. Further, <jats:italic>SOCS3<\/jats:italic> is mainly expressed in the nucleus as determined by immunofluorescence assay. Overexpression of <jats:italic>SOCS3<\/jats:italic> in DF-1 cells promoted the replication of ALV-J virus, and the expression of interferons (<jats:italic>IFN\u03b1<\/jats:italic> and <jats:italic>INF\u03b2<\/jats:italic>), inflammatory factors (<jats:italic>IL-6<\/jats:italic> and <jats:italic>TNF\u03b1<\/jats:italic>) along with interferon-stimulating genes (<jats:italic>CH25H<\/jats:italic>, <jats:italic>MX1<\/jats:italic>, <jats:italic>OASL<\/jats:italic>, and <jats:italic>ZAP<\/jats:italic>). Conversely, interference of <jats:italic>SOCS3<\/jats:italic> showed the opposite results. We also observed that SOCS3 promoted ALV-J virus replication by inhibiting JAK2\/STAT3 phosphorylation. In conclusion, <jats:italic>SOCS3<\/jats:italic> promotes ALV-J replication <jats:italic>via<\/jats:italic> inhibiting the phosphorylation of the JAK2\/STAT3 signaling pathway. These results would advance further understanding of the persistent infection and the viral immune evasion of the ALV-J virus.<\/jats:p>","DOI":"10.3389\/fcimb.2021.748795","type":"journal-article","created":{"date-parts":[[2021,9,10]],"date-time":"2021-09-10T05:23:19Z","timestamp":1631251399000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":31,"title":["SOCS3 Promotes ALV-J Virus Replication via Inhibiting JAK2\/STAT3 Phosphorylation During Infection"],"prefix":"10.3389","volume":"11","author":[{"given":"Guodong","family":"Mo","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huali","family":"Fu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bowen","family":"Hu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qihong","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mingjian","family":"Xian","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zihao","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ling","family":"Lin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meiqing","family":"Shi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qinghua","family":"Nie","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiquan","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2021,9,10]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"2393","DOI":"10.4049\/jimmunol.0903563","article-title":"Suppressor of Cytokine Signaling 3 Inhibits Antiviral IFN-\u03b2 Signaling to Enhance HIV-1 Replication in Macrophages","volume":"185","author":"Akhtar","year":"2010","journal-title":"J. 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