{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,30]],"date-time":"2026-05-30T00:43:56Z","timestamp":1780101836002,"version":"3.54.0"},"reference-count":80,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2022,1,6]],"date-time":"2022-01-06T00:00:00Z","timestamp":1641427200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004901","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de Minas Gerais","doi-asserted-by":"publisher","award":["APQ-02974-17"],"award-info":[{"award-number":["APQ-02974-17"]}],"id":[{"id":"10.13039\/501100004901","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["429635\/2018-4"],"award-info":[{"award-number":["429635\/2018-4"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Cell. Infect. Microbiol."],"abstract":"<jats:p><jats:italic>Trypanosoma cruzi<\/jats:italic> invades non-professional phagocytic cells by subverting their membrane repair process, which is dependent on membrane injury and cell signaling, intracellular calcium increase, and lysosome recruitment. Cells lacking lysosome-associated membrane proteins 1 and 2 (LAMP1 and LAMP2) are less permissive to parasite invasion but more prone to parasite intracellular multiplication. Several passages through a different intracellular environment can significantly change <jats:italic>T. cruzi<\/jats:italic>\u2019s gene expression profile. Here, we evaluated whether one single passage through LAMP-deficient (KO) or wild-type (WT) fibroblasts, thus different intracellular environments, could influence <jats:italic>T. cruzi<\/jats:italic> Y strain trypomastigotes\u2019 ability to invade L6 myoblasts and WT fibroblasts host cells. Parasites released from LAMP2 KO cells (TcY-L2<jats:sup>\u2212\/\u2212<\/jats:sup>) showed higher invasion, calcium signaling, and membrane injury rates, for the assays in L6 myoblasts, when compared to those released from WT (TcY-WT) or LAMP1\/2 KO cells (TcY-L1\/2<jats:sup>\u2212\/\u2212<\/jats:sup>). On the other hand, TcY-L1\/2<jats:sup>\u2212\/\u2212<\/jats:sup> showed higher invasion, calcium signaling, and cell membrane injury rates, for the assays in WT fibroblasts, compared to TcY-WT and TcY-L1\/2<jats:sup>\u2212\/\u2212<\/jats:sup>. Albeit TcY-WT presented an intermediary invasion and calcium signaling rates, compared to the others, in WT fibroblasts, they induced lower levels of injury, which reinforces that signals mediated by surface membrane protein interactions also have a significant contribution to trigger host cell calcium signals. These results clearly show that parasites released from WT or LAMP KO cells are distinct from each other. Additionally, these parasites\u2019 ability to invade the cell may be distinct depending on which cell type they interact with. Since these alterations most likely would reflect differences among parasite surface molecules, we also evaluated their proteome. We identified few protein complexes, membrane, and secreted proteins regulated in our dataset. Among those are some members of MASP, mucins, trans-sialidases, and gp63 proteins family, which are known to play an important role during parasite infection and could correlate to TcY-WT, TcY-L1\/2<jats:sup>\u2212\/\u2212<\/jats:sup>, and TcY-L2<jats:sup>\u2212\/\u2212<\/jats:sup> biological behavior.<\/jats:p>","DOI":"10.3389\/fcimb.2021.788482","type":"journal-article","created":{"date-parts":[[2022,1,6]],"date-time":"2022-01-06T05:35:51Z","timestamp":1641447351000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":5,"title":["Biological and Molecular Effects of Trypanosoma cruzi Residence in a LAMP-Deficient Intracellular Environment"],"prefix":"10.3389","volume":"11","author":[{"given":"Anny Carolline Silva","family":"Oliveira","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luisa","family":"Rezende","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vladimir","family":"Gorshkov","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcella Nunes","family":"Melo-Braga","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thiago","family":"Verano-Braga","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weslley","family":"Fernandes-Braga","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jorge Lu\u00eds de Melo","family":"Guadalupe","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gustavo Batista","family":"de Menezes","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Frank","family":"Kjeldsen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H\u00e9lida Monteiro","family":"de Andrade","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luciana de Oliveira","family":"Andrade","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2022,1,6]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"143","DOI":"10.1016\/S0166-6851(01)00245-6","article-title":"The Mucin-Like Glycoprotein Super-Family of Trypanosoma Cruzi: Structure and Biological Roles","volume":"114","author":"Acosta-Serrano","year":"2001","journal-title":"Mol. Biochem. Parasitol."},{"key":"B2","doi-asserted-by":"publisher","first-page":"784","DOI":"10.1016\/j.micinf.2010.05.015","article-title":"Role of Host Lysosomal Associated Membrane Protein (LAMP) in Trypanosoma Cruzi Invasion and Intracellular Development","volume":"12","author":"Albertti","year":"2010","journal-title":"Microbes Infection"},{"key":"B3","doi-asserted-by":"publisher","first-page":"3387","DOI":"10.1093\/bioinformatics\/btx431","article-title":"DeepLoc: Prediction of Protein Subcellular Localization Using Deep Learning","volume":"33","author":"Almagro Armenteros","year":"2017","journal-title":"Bioinformatics"},{"key":"B4","doi-asserted-by":"publisher","first-page":"420","DOI":"10.1038\/s41587-019-0036-z","article-title":"SignalP 5.0 Improves Signal Peptide Predictions Using Deep Neural Networks","volume":"37","author":"Almagro Armenteros","year":"2019","journal-title":"Nat. Biotechnol."},{"key":"B5","doi-asserted-by":"publisher","first-page":"274","DOI":"10.1080\/15216540701200084","article-title":"Trypanosoma Cruzi: Adhesion to the Host Cell and Intracellular Survival","volume":"59","author":"Alves","year":"2007","journal-title":"IUBMB Life"},{"key":"B6","doi-asserted-by":"publisher","first-page":"1135","DOI":"10.1084\/jem.20041408","article-title":"Lysosomal Fusion Is Essential for the Retention of Trypanosoma Cruzi Inside Host Cells","volume":"200","author":"Andrade","year":"2004","journal-title":"J. Exp. Med."},{"key":"B7","doi-asserted-by":"publisher","first-page":"819","DOI":"10.1038\/nrmicro1249","article-title":"The Trypanosoma Cruzi-Host-Cell Interplay: Location, Invasion, Retention","volume":"3","author":"Andrade","year":"2005","journal-title":"Nat. Rev. Microbiol."},{"key":"B8","doi-asserted-by":"publisher","first-page":"163","DOI":"10.1016\/S0166-6851(99)90035-X","article-title":"Differential Tissue Distribution of Diverse Clones of Trypanosoma Cruzi in Infected Mice","volume":"100","author":"Andrade","year":"1999","journal-title":"Mol. Biochem. Parasitol."},{"key":"B9","doi-asserted-by":"publisher","first-page":"834","DOI":"10.1590\/S0074-02762010000600018","article-title":"Differential Tissue Tropism of Trypanosoma Cruzi Strains: An In Vitro Study","volume":"105","author":"Andrade","year":"2010","journal-title":"Mem. Inst. Oswaldo Cruz"},{"key":"B10","doi-asserted-by":"publisher","first-page":"264","DOI":"10.1111\/j.1550-7408.1982.tb04024.x","article-title":"Adhesion and Interiorization of Trypanosoma Cruzi in Mammalian Cells","volume":"29","author":"Andrews","year":"1982","journal-title":"J. Protozool."},{"key":"B11","doi-asserted-by":"publisher","first-page":"474","DOI":"10.1016\/0014-4894(87)90062-2","article-title":"Stage-Specific Surface Antigens Expressed During the Morphogenesis of Vertebrate Forms of Trypanosoma Cruzi","volume":"64","author":"Andrews","year":"1987","journal-title":"Exp. Parasitol."},{"key":"B12","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2013.00186","article-title":"Trypanosoma Cruzi: Entry Into Mammalian Host Cells and Parasitophorous Vacuole Formation","volume":"4","author":"Barrias","year":"2013","journal-title":"Front. Immunol."},{"key":"B13","doi-asserted-by":"publisher","first-page":"883","DOI":"10.1021\/pr300947g","article-title":"Proteomic Analysis of Trypanosoma Cruzi Secretome: Characterization of Two Populations of Extracellular Vesicles and Soluble Proteins","volume":"12","author":"Bayer-Santos","year":"2013","journal-title":"J. Proteome Res."},{"key":"B14","doi-asserted-by":"publisher","first-page":"e1006767","DOI":"10.1371\/journal.ppat.1006767","article-title":"Comparative Transcriptome Profiling of Virulent and Non-Virulent Trypanosoma Cruzi Underlines the Role of Surface Proteins During Infection","volume":"13","author":"Belew","year":"2017","journal-title":"PloS Pathog."},{"key":"B15","doi-asserted-by":"publisher","first-page":"58","DOI":"10.1186\/1471-2180-5-58","article-title":"Non-Classical Protein Secretion in Bacteria","volume":"5","author":"Bendtsen","year":"2005","journal-title":"BMC Microbiol."},{"key":"B16","doi-asserted-by":"publisher","first-page":"255","DOI":"10.1042\/bj3100255","article-title":"Structural Analysis of Inositol Phospholipids From Trypanosoma Cruzi Epimastigote Forms","volume":"310","author":"Bertello","year":"1995","journal-title":"Biochem. J."},{"key":"B17","doi-asserted-by":"publisher","first-page":"111324","DOI":"10.1016\/j.molbiopara.2020.111324","article-title":"Lipid Metabolism in Trypanosoma Cruzi: A Review","volume":"240","author":"Booth","year":"2020","journal-title":"Mol. Biochem. Parasitol."},{"key":"B18","doi-asserted-by":"publisher","first-page":"248","DOI":"10.1016\/0003-2697(76)90527-3","article-title":"A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye Binding","volume":"72","author":"Bradford","year":"1976","journal-title":"Anal. Biochem."},{"key":"B19","doi-asserted-by":"publisher","first-page":"701","DOI":"10.1046\/j.1462-5822.2002.00226.x","article-title":"Cell Signalling and Trypanosoma Cruzi Invasion","volume":"4","author":"Burleigh","year":"2002","journal-title":"Cell Microbiol."},{"key":"B20","doi-asserted-by":"publisher","first-page":"229","DOI":"10.1038\/nrmicro1351","article-title":"Trypanosoma Cruzi Surface Mucins: Host-Dependent Coat Diversity","volume":"4","author":"Buscaglia","year":"2006","journal-title":"Nat. Rev. Microbiol."},{"key":"B21","doi-asserted-by":"publisher","first-page":"46","DOI":"10.1186\/1471-2091-12-46","article-title":"The Major Leucyl Aminopeptidase of Trypanosoma Cruzi (LAPTc) Assembles Into a Homohexamer and Belongs to the M17 Family of Metallopeptidases","volume":"12","author":"Cadavid-Restrepo","year":"2011","journal-title":"BMC Biochem."},{"key":"B22","doi-asserted-by":"crossref","first-page":"14631","DOI":"10.1038\/s41598-018-32877-2","article-title":"Genomic Assemblies of Newly Sequenced Trypanosoma Cruzi Strains Reveal New Genomic Expansion and Greater Complexity","volume":"8","author":"Callejas-Hernandez","year":"2018","journal-title":"Sci. Rep."},{"key":"B23","doi-asserted-by":"publisher","first-page":"1495","DOI":"10.1002\/cbic.201400069","article-title":"Antibodies Against Mucin-Based Glycopeptides Affect Trypanosoma Cruzi Cell Invasion and Tumor Cell Viability","volume":"15","author":"Campo","year":"2014","journal-title":"Chembiochem"},{"key":"B24","doi-asserted-by":"crossref","first-page":"e0005657","DOI":"10.1371\/journal.pntd.0005657","article-title":"LAMP-2 Absence Interferes With Plasma Membrane Repair and Decreases T. Cruzi Host Cell Invasion","volume":"11","author":"Couto","year":"2017","journal-title":"PloS Negl. Trop. Dis."},{"key":"B25","doi-asserted-by":"publisher","first-page":"5739","DOI":"10.1128\/IAI.71.10.5739-5749.2003","article-title":"Gp63 Homologues in Trypanosoma Cruzi: Surface Antigens With Metalloprotease Activity and a Possible Role in Host Cell Infection","volume":"71","author":"Cuevas","year":"2003","journal-title":"Infect. Immun."},{"key":"B26","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1155\/2010\/295394","article-title":"Review on Trypanosoma Cruzi: Host Cell Interaction","volume":"2010","author":"de Souza","year":"2010","journal-title":"Int. J. Cell Biol."},{"key":"B27","first-page":"D1145","article-title":"The ProteomeXchange Consortium in 2020: Enabling 'Big Data' Approaches in Proteomics","volume":"48","author":"Deutsch","year":"2020","journal-title":"Nucleic Acids Res."},{"key":"B28","doi-asserted-by":"publisher","first-page":"e1779","DOI":"10.1371\/journal.pntd.0001779","article-title":"The MASP Family of Trypanosoma Cruzi: Changes in Gene Expression and Antigenic Profile During the Acute Phase of Experimental Infection","volume":"6","author":"dos Santos","year":"2012","journal-title":"PloS Neglected Trop. Dis."},{"key":"B29","doi-asserted-by":"publisher","first-page":"1155","DOI":"10.1093\/protein\/11.12.1155","article-title":"Sequence Properties of GPI-Anchored Proteins Near the Omega-Site: Constraints for the Polypeptide Binding Site of the Putative Transamidase","volume":"11","author":"Eisenhaber","year":"1998","journal-title":"Protein Eng."},{"key":"B30","doi-asserted-by":"publisher","first-page":"741","DOI":"10.1006\/jmbi.1999.3069","article-title":"Prediction of Potential GPI-Modification Sites in Proprotein Sequences","volume":"292","author":"Eisenhaber","year":"1999","journal-title":"J. Mol. Biol."},{"key":"B31","doi-asserted-by":"publisher","first-page":"340","DOI":"10.1016\/S0968-0004(00)01601-7","article-title":"Automated Annotation of GPI Anchor Sites: Case Study C. Elegans","volume":"25","author":"Eisenhaber","year":"2000","journal-title":"Trends Biochem. Sci."},{"key":"B32","doi-asserted-by":"publisher","first-page":"495","DOI":"10.1016\/j.mam.2006.08.005","article-title":"Roles of LAMP-1 and LAMP-2 in Lysosome Biogenesis and Autophagy","volume":"27","author":"Eskelinen","year":"2006","journal-title":"Mol. Aspects Med."},{"key":"B33","doi-asserted-by":"publisher","first-page":"3355","DOI":"10.1091\/mbc.e02-02-0114","article-title":"Role of LAMP-2 in Lysosome Biogenesis and Autophagy","volume":"13","author":"Eskelinen","year":"2002","journal-title":"Mol. Biol. Cell"},{"key":"B34","doi-asserted-by":"publisher","first-page":"3132","DOI":"10.1091\/mbc.e04-02-0103","article-title":"Disturbed Cholesterol Traffic But Normal Proteolytic Function in LAMP-1\/LAMP-2 Double-Deficient Fibroblasts","volume":"15","author":"Eskelinen","year":"2004","journal-title":"Mol. Biol. Cell"},{"key":"B35","doi-asserted-by":"publisher","first-page":"2799","DOI":"10.1242\/jcs.112.17.2799","article-title":"The Structure, Biosynthesis and Functions of Glycosylphosphatidylinositol Anchors, and the Contributions of Trypanosome Research","volume":"112","author":"Ferguson","year":"1999","journal-title":"J. Cell Sci."},{"key":"B36","doi-asserted-by":"publisher","first-page":"734","DOI":"10.1111\/j.1574-6976.2012.00333.x","article-title":"Host Cell Invasion by Trypanosoma Cruzi: A Unique Strategy That Promotes Persistence","volume":"36","author":"Fernandes","year":"2012","journal-title":"FEMS Microbiol. Rev."},{"key":"B37","doi-asserted-by":"publisher","first-page":"909","DOI":"10.1084\/jem.20102518","article-title":"Trypanosoma Cruzi Subverts the Sphingomyelinase-Mediated Plasma Membrane Repair Pathway for Cell Invasion","volume":"208","author":"Fernandes","year":"2011","journal-title":"J. Exp. Med."},{"key":"B38","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2021.634793","article-title":"All Roads Lead to Cytosol: Trypanosoma Cruzi Multi-Strategic Approach to Invasion","volume":"11","author":"Ferri","year":"2021","journal-title":"Front. Cell Infect. Microbiol."},{"key":"B39","doi-asserted-by":"publisher","first-page":"519","DOI":"10.1042\/BJ20041842","article-title":"Characterization of the Inositol Phosphorylceramide Synthase Activity From Trypanosoma Cruzi","volume":"387","author":"Figueiredo","year":"2005","journal-title":"Biochem. J."},{"key":"B40","doi-asserted-by":"publisher","first-page":"895","DOI":"10.1038\/nri1978","article-title":"Protozoan Encounters With Toll-Like Receptor Signalling Pathways: Implications for Host Parasitism","volume":"6","author":"Gazzinelli","year":"2006","journal-title":"Nat. Rev. Immunol."},{"key":"B41","doi-asserted-by":"publisher","first-page":"598","DOI":"10.1590\/S0074-02762008000600015","article-title":"Trypanosoma Cruzi: A Stage-Specific Calpain-Like Protein Is Induced After Various Kinds of Stress","volume":"103","author":"Giese","year":"2008","journal-title":"Mem. Inst. Oswaldo Cruz"},{"key":"B42","doi-asserted-by":"publisher","first-page":"1389","DOI":"10.1016\/j.carres.2011.04.006","article-title":"Trans-Sialidase and Mucins of Trypanosoma Cruzi: An Important Interplay for the Parasite","volume":"346","author":"Giorgi","year":"2011","journal-title":"Carbohydr. Res."},{"key":"B43","doi-asserted-by":"publisher","first-page":"43","DOI":"10.1016\/0014-4894(91)90119-H","article-title":"Trypanosoma Cruzi: Shedding of Surface Antigens as Membrane Vesicles","volume":"72","author":"Goncalves","year":"1991","journal-title":"Exp. Parasitol."},{"key":"B44","doi-asserted-by":"publisher","first-page":"16877","DOI":"10.1074\/jbc.271.28.16877","article-title":"Identification of Complete Precursors for the Glycosylphosphatidylinositol Protein Anchors of Trypanosoma Cruzi","volume":"271","author":"Heise","year":"1996","journal-title":"J. Biol. Chem."},{"key":"B45","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1016\/S0166-6851(01)00296-1","article-title":"CAP5.5, a Life-Cycle-Regulated, Cytoskeleton-Associated Protein Is a Member of a Novel Family of Calpain-Related Proteins in Trypanosoma Brucei","volume":"116","author":"Hertz-Fowler","year":"2001","journal-title":"Mol. Biochem. Parasitol."},{"key":"B46","doi-asserted-by":"publisher","first-page":"483","DOI":"10.1146\/annurev.cb.05.110189.002411","article-title":"The Biogenesis of Lysosomes","volume":"5","author":"Kornfeld","year":"1989","journal-title":"Annu. Rev. Cell. Biol."},{"key":"B47","doi-asserted-by":"publisher","first-page":"567","DOI":"10.1006\/jmbi.2000.4315","article-title":"Predicting Transmembrane Protein Topology With a Hidden Markov Model: Application to Complete Genomes","volume":"305","author":"Krogh","year":"2001","journal-title":"J. Mol. Biol."},{"key":"B48","doi-asserted-by":"publisher","first-page":"2193","DOI":"10.1128\/IAI.01542-08","article-title":"Trypanosoma Cruzi GP63 Proteins Undergo Stage-Specific Differential Posttranslational Modification and Are Important for Host Cell Infection","volume":"77","author":"Kulkarni","year":"2009","journal-title":"Infect. Immun."},{"key":"B49","doi-asserted-by":"publisher","first-page":"24","DOI":"10.1006\/abio.1997.2170","article-title":"Zymography: A Single-Step Staining Method for Quantitation of Proteolytic Activity on Substrate Gels","volume":"249","author":"Leber","year":"1997","journal-title":"Anal. Biochem."},{"key":"B50","first-page":"253","article-title":"Sphingolipids With Inositolphosphate-Containing Head Groups","volume":"26","author":"Lester","year":"1993","journal-title":"Adv. Lipid Res."},{"key":"B51","doi-asserted-by":"publisher","DOI":"10.3791\/3149","article-title":"Cell-Based Calcium Assay for Medium to High Throughput Screening of TRP Channel Functions Using FlexStation 3","volume":"54","author":"Luo","year":"2011","journal-title":"J. Vis. Exp."},{"key":"B52","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1590\/S0074-02762004000100001","article-title":"Trypanosoma Cruzi: Genetic Structure of Populations and Relevance of Genetic Variability to the Pathogenesis of Chagas Disease","volume":"99","author":"Macedo","year":"2004","journal-title":"Mem. Inst. Oswaldo Cruz"},{"key":"B53","doi-asserted-by":"publisher","first-page":"189","DOI":"10.1186\/s13071-016-1455-z","article-title":"Host Cell Invasion and Oral Infection by Trypanosoma Cruzi Strains of Genetic Groups TcI and TcIV From Chagasic Patients","volume":"9","author":"Maeda","year":"2016","journal-title":"Parasit. Vectors"},{"key":"B54","doi-asserted-by":"publisher","first-page":"3023","DOI":"10.1128\/IAI.00138-09","article-title":"Use of L-Proline and ATP Production by Trypanosoma Cruzi Metacyclic Forms as Requirements for Host Cell Invasion","volume":"77","author":"Martins","year":"2009","journal-title":"Infect. Immun."},{"key":"B55","doi-asserted-by":"publisher","first-page":"305","DOI":"10.1042\/bj2940305","article-title":"The Structure, Biosynthesis and Function of Glycosylated Phosphatidylinositols in the Parasitic Protozoa and Higher Eukaryotes","volume":"294","author":"McConville","year":"1993","journal-title":"Biochem. J."},{"key":"B56","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1080\/096876800294443","article-title":"Recent Developments in the Cell Biology and Biochemistry of Glycosylphosphatidylinositol Lipids (Review)","volume":"17","author":"McConville","year":"2000","journal-title":"Mol. Membr. Biol."},{"key":"B57","doi-asserted-by":"publisher","first-page":"1162","DOI":"10.1111\/liv.12587","article-title":"Calcium Signalling From the Type I Inositol 1,4,5-Trisphosphate Receptor Is Required at Early Phase of Liver Regeneration","volume":"35","author":"Oliveira","year":"2015","journal-title":"Liver Int."},{"key":"B58","doi-asserted-by":"publisher","first-page":"e69419","DOI":"10.1371\/journal.pone.0069419","article-title":"Proline Dehydrogenase Regulates Redox State and Respiratory Metabolism in Trypanosoma Cruzi","volume":"8","author":"Paes","year":"2013","journal-title":"PloS One"},{"key":"B59","doi-asserted-by":"publisher","first-page":"D442","DOI":"10.1093\/nar\/gky1106","article-title":"The PRIDE Database and Related Tools and Resources in 2019: Improving Support for Quantification Data","volume":"47","author":"Perez-Riverol","year":"2019","journal-title":"Nucleic Acids Res."},{"key":"B60","doi-asserted-by":"publisher","first-page":"479","DOI":"10.1002\/jbt.21507","article-title":"The Cytotoxic and Proapoptotic Activities of Hypnophilin Are Associated With Calcium Signaling in UACC-62 Cells","volume":"27","author":"Pinto","year":"2013","journal-title":"J. Biochem. Mol. Toxicol."},{"key":"B61","doi-asserted-by":"publisher","first-page":"67","DOI":"10.1016\/0166-6851(82)90066-4","article-title":"Changes in Morphology and Infectivity of Cell Culture-Derived Trypomastigotes of Trypanosoma Cruzi","volume":"6","author":"Piras","year":"1982","journal-title":"Mol. Biochem. Parasitol."},{"key":"B62","doi-asserted-by":"publisher","first-page":"31","DOI":"10.1002\/9780470720806.ch3","article-title":"Trypanosoma Cruzi-Fibroblastic Cell Interactions Necessary for Cellular Invasion","volume":"99","author":"Piras","year":"1983","journal-title":"Ciba Found Symp."},{"key":"B63","first-page":"1","article-title":"Glycoinositolphospholipid From Trypanosoma Cruzi: Structure, Biosynthesis and Immunobiology","volume":"56","author":"Previato","year":"2004","journal-title":"Adv. Parasitol."},{"key":"B64","doi-asserted-by":"publisher","first-page":"3530","DOI":"10.1021\/pr401120y","article-title":"Cell Surface Proteome Analysis of Human-Hosted Trypanosoma Cruzi Life Stages","volume":"13","author":"Queiroz","year":"2014","journal-title":"J. Proteome Res."},{"key":"B65","doi-asserted-by":"publisher","first-page":"1075","DOI":"10.1017\/S0031182019000441","article-title":"Participation of Trypanosoma Cruzi Gp63 Molecules on the Interaction With Rhodnius Prolixus","volume":"146","author":"Rebello","year":"2019","journal-title":"Parasitology"},{"key":"B66","doi-asserted-by":"publisher","first-page":"6","DOI":"10.4161\/auto.5.1.7160","article-title":"The Autophagic Pathway Is a Key Component in the Lysosomal Dependent Entry of Trypanosoma Cruzi Into the Host Cell","volume":"5","author":"Romano","year":"2009","journal-title":"Autophagy"},{"key":"B67","doi-asserted-by":"publisher","first-page":"645","DOI":"10.1128\/iai.59.2.645-654.1991","article-title":"Attachment of Trypanosoma Cruzi To Mammalian Cells Requires Parasite Energy, and Invasion can be Independent of the Target Cell Cytoskeleton","volume":"59","author":"Schenkman","year":"1991","journal-title":"Infect. Immm."},{"key":"B68","doi-asserted-by":"publisher","first-page":"280","DOI":"10.1111\/j.1582-4934.2009.00973.x","article-title":"Role for LAMP-2 in Endosomal Cholesterol Transport","volume":"15","author":"Schneede","year":"2011","journal-title":"J. Cell Mol. Med."},{"key":"B69","doi-asserted-by":"publisher","first-page":"87","DOI":"10.4269\/ajtmh.2012.12-0015","article-title":"Intraspecies Variation in Trypanosoma Cruzi GPI-Mucins: Biological Activities and Differential Expression of Alpha-Galactosyl Residues","volume":"87","author":"Soares","year":"2012","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"B70","first-page":"175","article-title":"A Hidden Markov Model for Predicting Transmembrane Helices in Protein Sequences","volume":"6","author":"Sonnhammer","year":"1998","journal-title":"Proc. Int. Conf. Intell. Syst. Mol. Biol."},{"key":"B71","doi-asserted-by":"publisher","first-page":"387","DOI":"10.1093\/protein\/12.5.387","article-title":"PSIC: Profile Extraction From Sequence Alignments With Position-Specific Counts of Independent Observations","volume":"12","author":"Sunyaev","year":"1999","journal-title":"Protein Eng."},{"key":"B72","doi-asserted-by":"publisher","first-page":"e21800","DOI":"10.1371\/journal.pone.0021800","article-title":"REVIGO Summarizes and Visualizes Long Lists of Gene Ontology Terms","volume":"6","author":"Supek","year":"2011","journal-title":"PloS One"},{"key":"B73","doi-asserted-by":"publisher","first-page":"1027","DOI":"10.1083\/jcb.201003053","article-title":"Exocytosis of Acid Sphingomyelinase by Wounded Cells Promotes Endocytosis and Plasma Membrane Repair","volume":"189","author":"Tam","year":"2010","journal-title":"J. Cell Biol."},{"key":"B74","doi-asserted-by":"publisher","first-page":"902","DOI":"10.1038\/35022595","article-title":"Accumulation of Autophagic Vacuoles and Cardiomyopathy in LAMP-2-Deficient Mice","volume":"406","author":"Tanaka","year":"2000","journal-title":"Nature"},{"key":"B75","doi-asserted-by":"publisher","first-page":"1117","DOI":"10.1016\/S0092-8674(05)80061-3","article-title":"Lysosome Recruitment and Fusion Are Early Events Required for Trypanosome Invasion of Mammalian Cells","volume":"71","author":"Tardieux","year":"1992","journal-title":"Cell"},{"key":"B76","doi-asserted-by":"publisher","first-page":"1017","DOI":"10.1084\/jem.179.3.1017","article-title":"Role in Host Cell Invasion of Trypanosoma Cruzi-Induced Cytosolic-Free Ca2+ Transients","volume":"179","author":"Tardieux","year":"1994","journal-title":"J. Exp. Med."},{"key":"B77","doi-asserted-by":"publisher","first-page":"733","DOI":"10.1111\/j.1462-5822.2004.00397.x","article-title":"L-Proline Is Essential for the Intracellular Differentiation of Trypanosoma Cruzi","volume":"6","author":"Tonelli","year":"2004","journal-title":"Cell Microbiol."},{"key":"B78","doi-asserted-by":"publisher","first-page":"29","DOI":"10.1016\/j.micinf.2008.10.003","article-title":"Trypanosoma Cruzi: Parasite Shed Vesicles Increase Heart Parasitism and Generate an Intense Inflammatory Response","volume":"11","author":"Trocoli Torrecilhas","year":"2009","journal-title":"Microbes Infect."},{"key":"B79","doi-asserted-by":"publisher","first-page":"233","DOI":"10.1016\/0005-2760(96)00021-5","article-title":"Characterization of Inositolphospholipids in Trypanosoma Cruzi Trypomastigote Forms","volume":"1300","author":"Uhrig","year":"1996","journal-title":"Biochim. Biophys. Acta"},{"key":"B80","volume-title":"Chagas Disease (American Trypanosomiasis)","year":"2021"}],"container-title":["Frontiers in Cellular and Infection Microbiology"],"original-title":[],"link":[{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fcimb.2021.788482\/full","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,1,6]],"date-time":"2022-01-06T05:36:01Z","timestamp":1641447361000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fcimb.2021.788482\/full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,1,6]]},"references-count":80,"alternative-id":["10.3389\/fcimb.2021.788482"],"URL":"https:\/\/doi.org\/10.3389\/fcimb.2021.788482","relation":{},"ISSN":["2235-2988"],"issn-type":[{"value":"2235-2988","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,1,6]]},"article-number":"788482"}}