{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,26]],"date-time":"2026-07-26T23:58:44Z","timestamp":1785110324688,"version":"3.55.0"},"reference-count":40,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2022,1,18]],"date-time":"2022-01-18T00:00:00Z","timestamp":1642464000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Cell. Infect. Microbiol."],"abstract":"<jats:p><jats:italic>Burkholderia pseudomallei<\/jats:italic> is an important infectious disease pathogen that can cause melioidosis. Melioidosis is mainly prevalent in Thailand, northern Australia and southern China and has become a global public health problem. Early identification of <jats:italic>B. pseudomallei<\/jats:italic> is of great significance for the diagnosis and prognosis of melioidosis. In this study, a simple and visual device combined with lateral flow strip-based recombinase polymerase amplification (LF-RPA) was developed, and the utility of the LF-RPA assay for identifying <jats:italic>B. pseudomallei<\/jats:italic> was evaluated. In order to screen out the optimal primer probe, a total of 16 pairs of specific primers targeting the <jats:italic>orf2<\/jats:italic> gene of <jats:italic>B. pseudomallei<\/jats:italic> type III secretion system (T3SS) cluster genes were designed for screening, and F1\/R3 was selected as an optimal set of primers for the identification of <jats:italic>B. pseudomallei<\/jats:italic>, and parameters for LF-RPA were optimized. The LF-RPA can be amplified at 30-45\u00b0C and complete the entire reaction in 5-30 min. This reaction does not cross-amplify the DNA of other non-<jats:italic>B. pseudomallei<\/jats:italic> species. The limit of detection (LOD) of this assay for <jats:italic>B. pseudomallei<\/jats:italic> genomic DNA was as low as 30 femtograms (fg), which was comparable to the results of real-time PCR. Moreover, 21 clinical <jats:italic>B. pseudomallei<\/jats:italic> isolates identified by 16S rRNA gene sequencing were retrospectively confirmed by the newly developed LF-RPA system. Our results showed that the newly developed LF-RPA system has a simple and short time of operation and has good application prospect in the identification of <jats:italic>B. pseudomallei.<\/jats:italic><\/jats:p>","DOI":"10.3389\/fcimb.2021.804737","type":"journal-article","created":{"date-parts":[[2022,1,18]],"date-time":"2022-01-18T05:36:10Z","timestamp":1642484170000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":11,"title":["Preliminary Evaluation of Rapid Visual Identification of Burkholderia pseudomallei Using a Newly Developed Lateral Flow Strip-Based Recombinase Polymerase Amplification (LF-RPA) System"],"prefix":"10.3389","volume":"11","author":[{"given":"Jin","family":"Li","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiu","family":"Zhong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mei-Yun","family":"Shang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Min","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuan-Su","family":"Jiang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jia-Jun","family":"Zou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shan-Shan","family":"Ma","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qing","family":"Huang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei-Ping","family":"Lu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2022,1,18]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"327","DOI":"10.31128\/AJGP-04-18-4558","article-title":"Melioidosis: An Updated Review","volume":"48","author":"Chakravorty","year":"2019","journal-title":"Aust. 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