{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T21:01:02Z","timestamp":1790802062472,"version":"4.1.0"},"reference-count":66,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2022,10,13]],"date-time":"2022-10-13T00:00:00Z","timestamp":1665619200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Cell. Infect. Microbiol."],"abstract":"<jats:p>The <jats:italic>Toxoplasma gondii<\/jats:italic> tachyzoite is a singled-cell obligate intracellular parasite responsible for the acute phase of toxoplasmosis. This polarized cell exhibits an apical complex, a hallmark of the phylum Apicomplexa, essential for motility, invasion, and egress from the host cell. Located on the opposite end of the cell is the basal complex, an elaborated cytoskeletal structure that also plays critical roles in the lytic cycle of the parasite, being involved in motility, cell division, constriction and cytokinesis, as well as intravacuolar cell-cell communication. Nevertheless, only a few proteins of this structure have been described and functionally assessed. In this study, we used spatial proteomics to identify new basal complex components (BCC), and <jats:italic>in situ<\/jats:italic> imaging, including ultrastructure expansion microscopy, to position them. We thus confirmed the localization of nine BCCs out of the 12 selected candidates and assigned them to different sub-compartments of the basal complex, including two new domains located above the basal ring and below the posterior cup. Their functional investigation revealed that none of these BCCs are essential for parasite growth <jats:italic>in vitro<\/jats:italic>. However, one BCC is critical for constricting of the basal complex, likely through direct interaction with the class VI myosin heavy chain J (MyoJ), and for gliding motility. Four other BCCs, including a phosphatase and a guanylate-binding protein, are involved in the formation and\/or maintenance of the intravacuolar parasite connection, which is required for the rosette organization and synchronicity of cell division.<\/jats:p>","DOI":"10.3389\/fcimb.2022.1010038","type":"journal-article","created":{"date-parts":[[2022,10,13]],"date-time":"2022-10-13T15:29:51Z","timestamp":1665674991000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":30,"title":["Identification of new components of the basal pole of Toxoplasma gondii provides novel insights into its molecular organization and functions"],"prefix":"10.3389","volume":"12","author":[{"given":"Chlo\u00e9","family":"Roum\u00e9gous","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aya","family":"Abou Hammoud","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Damien","family":"Fuster","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean-William","family":"Dupuy","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Corinne","family":"Blancard","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B\u00e9n\u00e9dicte","family":"Salin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Derrick R.","family":"Robinson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patricia","family":"Renesto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isabelle","family":"Tardieux","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karine","family":"Fr\u00e9nal","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2022,10,13]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0114628","article-title":"Triacylglycerol storage in lipid droplets in procyclic Trypanosoma brucei","volume":"9","author":"Allmann","year":"2014","journal-title":"PLoS One"},{"key":"B2","doi-asserted-by":"publisher","first-page":"18","DOI":"10.1111\/j.1462-5822.2010.01514.x","article-title":"A family of intermediate filament-like proteins is sequentially assembled into the cytoskeleton of Toxoplasma gondii","volume":"13","author":"Anderson-White","year":"2011","journal-title":"Cell Microbiol."},{"key":"B3","doi-asserted-by":"publisher","first-page":"752","DOI":"10.1016\/j.chom.2020.09.011","article-title":"A comprehensive subcellular atlas of the Toxoplasma proteome via hyperLOPIT provides spatial context for protein functions","volume":"28","author":"Barylyuk","year":"2020","journal-title":"Cell Host Microbe"},{"key":"B4","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0012354","article-title":"Coordinated progression through two subtranscriptomes underlies the tachyzoite cycle of Toxoplasma gondii","volume":"5","author":"Behnke","year":"2010","journal-title":"PLoS One"},{"key":"B5","doi-asserted-by":"publisher","first-page":"453","DOI":"10.1111\/mmi.14674","article-title":"Biogenesis and discharge of the rhoptries: Key organelles for entry and hijack of host cells by the apicomplexa","volume":"115","author":"Ben Chaabene","year":"2021","journal-title":"Mol. 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