{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,16]],"date-time":"2026-02-16T16:42:39Z","timestamp":1771260159151,"version":"3.50.1"},"reference-count":60,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2022,9,23]],"date-time":"2022-09-23T00:00:00Z","timestamp":1663891200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Cell. Infect. Microbiol."],"abstract":"<jats:p>The tumour necrosis factor superfamily OX40L and CD70 and their receptors are costimulatory signalling axes critical for adequate T and B cell activation in humans and mice. In this work we inoculated groups of sheep with human recombinant adenovirus type 5 (Ad) expressing<jats:italic>Ovis aries<\/jats:italic>(<jats:italic>Oa)<\/jats:italic>OX40L or<jats:italic>Oa<\/jats:italic>CD70 or a control adenoviral vector to determine whether they could improve the immune response to the model antigen OVA. PBMCs and serum samples were obtained for analysis of the adaptive immune response to OVA at days 0, 15, 30 and 90 post-inoculation (pi). Recall responses to OVA were assessed at day 7 and 30 after the second antigen inoculation (pb) at day 90. Administration of these immunomodulatory molecules did not induce unspecific PBMC stimulation. While<jats:italic>Oa<\/jats:italic>OX40L administration mainly increased TNF-\u03b1 and IL-4 in PBMC at day 15 pi concomitantly with a slight increase in antibody titer and the number of IFN-\u03b3 producing cells, we detected greater effects on adaptive immunity after<jats:italic>Oa<\/jats:italic>CD70 administration. Ad<jats:italic>Oa<\/jats:italic>CD70 inoculation improved antibody titers to OVA at days 30 and 90 pi, and increased anti-OVA-specific IgG-secreting B cell counts when compared to control. Moreover, higher IFN-\u03b3 production was detected on days 7 pi, 7 pb and 30 pb in PBMCs from this group. Phenotypic analysis of T cell activation showed an increase in effector CD8<jats:sup>+<\/jats:sup>T cells (CD8<jats:sup>+<\/jats:sup>CD62L<jats:sup>-<\/jats:sup>CD27<jats:sup>-<\/jats:sup>) at day 15 pi in Ad<jats:italic>Oa<\/jats:italic>CD70 group, concurrent with a decrease in early activated cells (CD8<jats:sup>+<\/jats:sup>CD62L<jats:sup>-<\/jats:sup>CD27<jats:sup>+<\/jats:sup>). Moreover, recall anti-OVA CD8<jats:sup>+<\/jats:sup>T cell responses were increased at 7 pb in the Ad<jats:italic>Oa<\/jats:italic>CD70 group. Ad<jats:italic>Oa<\/jats:italic>CD70 administration could therefore promote CD8<jats:sup>+<\/jats:sup>T cell effector differentiation and long-term activity. In this work we characterized the<jats:italic>in vivo<\/jats:italic>adjuvant potential on the humoral and cellular immune response of<jats:italic>Oa<\/jats:italic>OX40L and<jats:italic>Oa<\/jats:italic>CD70 delivered by non-replicative adenovirus vectors using the model antigen OVA. We present data highlighting the potency of these molecules as veterinary vaccine adjuvant.<\/jats:p>","DOI":"10.3389\/fcimb.2022.1010873","type":"journal-article","created":{"date-parts":[[2022,9,23]],"date-time":"2022-09-23T11:14:40Z","timestamp":1663931680000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":3,"title":["Adenoviral delivery of soluble ovine OX40L or CD70 costimulatory molecules improves adaptive immune responses to a model antigen in sheep"],"prefix":"10.3389","volume":"12","author":[{"given":"Jos\u00e9 M.","family":"Rojas","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carolina","family":"Mancho","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andr\u00e9s","family":"Louloudes-L\u00e1zaro","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniel","family":"Rodr\u00edguez-Mart\u00edn","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Miguel","family":"Avia","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Santiago","family":"Moreno","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Noem\u00ed","family":"Sevilla","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ver\u00f3nica","family":"Mart\u00edn","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1965","published-online":{"date-parts":[[2022,9,23]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"13353","DOI":"10.1074\/jbc.273.21.13353","article-title":"CD27, a member of the tumor necrosis factor receptor superfamily, activates NF-\u03baB and stress-activated protein kinase\/c-jun n-terminal kinase via TRAF2, TRAF5, and NF-\u03baB-inducing kinase","volume":"273","author":"Akiba","year":"1998","journal-title":"J. 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