{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T07:19:06Z","timestamp":1760080746439,"version":"3.41.2"},"reference-count":84,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2022,9,27]],"date-time":"2022-09-27T00:00:00Z","timestamp":1664236800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003141","name":"Consejo Nacional de Ciencia y Tecnolog\u00eda","doi-asserted-by":"publisher","award":["267746"],"award-info":[{"award-number":["267746"]}],"id":[{"id":"10.13039\/501100003141","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002347","name":"Bundesministerium f\u00fcr Bildung und Forschung","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002347","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100005156","name":"Alexander von Humboldt-Stiftung","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100005156","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003141","name":"Consejo Nacional de Ciencia y Tecnolog\u00eda","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003141","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Cell. Infect. Microbiol."],"abstract":"<jats:p>Human adenovirus 36 (HAdV-D36) can cause obesity in animal models, induces an adipogenic effect and increased adipocyte differentiation in cell culture. HAdV-D36 infection alters gene expression and the metabolism of the infected cells resulting in increased glucose internalization and triglyceride accumulation. Although HAdV-D36 prevalence correlates with obesity in humans, whether human preadipocytes may be targeted <jats:italic>in vivo<\/jats:italic> has not been determined and metabolic reprogramming of preadipocytes has not been explored in the context of the viral replication cycle. HAdV-D36 infection of the mouse fibroblasts, 3T3-L1 cells, which can differentiate into adipocytes, promotes proliferation and differentiation, but replication of the virus in these cells is abortive as indicated by short-lived transient expression of viral mRNA and a progressive loss of viral DNA. Therefore, we have evaluated whether a productive viral replication cycle can be established in the 3T3-L1 preadipocyte model under conditions that drive the cell differentiation process. For this purpose, viral mRNA levels and viral DNA replication were measured by RT-qPCR and qPCR, respectively, and viral progeny production was determined by plaque assay. The lipogenic effect of infection was evaluated with Oil Red O (ORO) staining, and expression of genes that control lipid and glucose metabolism was measured by RT-qPCR. In the context of a viral productive cycle, HAdV-D36 modulated the expression of the adipogenic genes, C\/EBP\u03b1, C\/EBP\u03b2 and PPAR\u03b3, as well as intracellular lipid accumulation, and the infection was accompanied by altered expression of glucolytic genes. The results show that only adipocyte-committed 3T3-L1 cells are permissive for the expression of early and late viral mRNAs, as well as viral DNA replication and progeny production, supporting productive HAdV-D36 viral replication, indicating that a greater effect on adipogenesis occurs in adipocytes that support productive viral replication.<\/jats:p>","DOI":"10.3389\/fcimb.2022.1016200","type":"journal-article","created":{"date-parts":[[2022,9,27]],"date-time":"2022-09-27T05:32:37Z","timestamp":1664256757000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":4,"title":["Adipocyte commitment of 3T3-L1 cells is required to support human adenovirus 36 productive replication concurrent with altered lipid and glucose metabolism"],"prefix":"10.3389","volume":"12","author":[{"given":"Ver\u00f3nica","family":"M\u00e1rquez","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Grisel","family":"Ballesteros","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thomas","family":"Dobner","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ram\u00f3n A.","family":"Gonz\u00e1lez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1965","published-online":{"date-parts":[[2022,9,27]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.1016\/j.heliyon.2020.e05261","article-title":"E4orf1, an adeno-viral protein, attenuates renal lipid accumulation in high fat fed mice: A novel approach to reduce a key risk factor for chronic kidney disease","volume":"6","author":"Afruza","year":"2020","journal-title":"Heliyon"},{"key":"B2","doi-asserted-by":"publisher","first-page":"1192","DOI":"10.4065\/82.10.1192","article-title":"Viruses as an etiology of obesity","volume":"82","author":"Atkinson","year":"2007","journal-title":"Mayo Clin. Proc."},{"key":"B3","doi-asserted-by":"publisher","first-page":"371","DOI":"10.1016\/0092-8674(81)90206-3","article-title":"The stability of early adenovirus mRNA is controlled by the viral 72 kd DNA-binding protein","volume":"26","author":"Babich","year":"1981","journal-title":"Cell"},{"key":"B4","doi-asserted-by":"publisher","first-page":"2552","DOI":"10.1128\/mcb.5.10.2552-2558.1985","article-title":"Adenovirus E1B proteins are required for accumulation of late viral mRNA and for effects on cellular mRNA translation and transport","volume":"5","author":"Babiss","year":"1985","journal-title":"Mol. Cell Biol."},{"key":"B5","article-title":"Adenoviridae chap. 55","author":"Berk","year":"2013","journal-title":"Howley, Fields Virology. 6th Edition"},{"key":"B6","doi-asserted-by":"publisher","first-page":"751S","DOI":"10.1093\/jn\/137.3.751S","article-title":"Metabolic diversity of the intestinal microbiota: implications for health and disease","volume":"137","author":"Blaut","year":"2007","journal-title":"J. Nutr."},{"key":"B7","doi-asserted-by":"publisher","first-page":"260","DOI":"10.1016\/0042-6822(91)90138-2","article-title":"Differential regulation of adenovirus late transcriptional units by the products of early region","volume":"183","author":"Bridge","year":"1991","journal-title":"Virology"},{"key":"B8","doi-asserted-by":"publisher","first-page":"195","DOI":"10.1002\/bit.26063","article-title":"(13) c-metabolic flux analysis of human adenovirus infection: Implications for viral vector production","volume":"114","author":"Carinhas","year":"2017","journal-title":"Biotechnol. Bioeng."},{"key":"B9","doi-asserted-by":"publisher","first-page":"2103","DOI":"10.1128\/jvi.64.5.2103-2109.1990","article-title":"The adenovirus DNA-binding protein stimulates the rate of transcription directed by adenovirus and adeno-associated virus promoters","volume":"64","author":"Chang","year":"1990","journal-title":"J. Virol."},{"key":"B10","doi-asserted-by":"publisher","first-page":"6","DOI":"10.1016\/j.metabol.2018.09.005","article-title":"The epidemiology of obesity","volume":"92","author":"Chooi","year":"2019","journal-title":"Metabolism"},{"key":"B11","doi-asserted-by":"publisher","first-page":"259","DOI":"10.1101\/sqb.2001.66.259","article-title":"Preferential translation of adenovirus mRNAs in infected cells","volume":"66","author":"Cuesta","year":"2001","journal-title":"Cold Spring Harb. Symp. Quant. Biol."},{"key":"B12","doi-asserted-by":"publisher","first-page":"2794S","DOI":"10.1093\/jn\/131.10.2794S","article-title":"Infectobesity: obesity of infectious origin","volume":"131","author":"Dhurandhar","year":"2001","journal-title":"J. Nutr."},{"key":"B13","doi-asserted-by":"publisher","first-page":"e23394","DOI":"10.1371\/journal.pone.0023394","article-title":"E4orf1: A novel ligand that improves glucose disposal in cell culture","volume":"6","author":"Dhurandhar","year":"2011","journal-title":"PloS One"},{"key":"B14","doi-asserted-by":"publisher","first-page":"989","DOI":"10.1038\/sj.ijo.0801319","article-title":"Increased adiposity in animals due to a human virus","volume":"24","author":"Dhurandhar","year":"2000","journal-title":"Int. J. Obes. Relat. Metab. Disord."},{"key":"B15","doi-asserted-by":"publisher","first-page":"990","DOI":"10.1038\/sj.ijo.0801668","article-title":"Transmissibility of adenovirus-induced adiposity in a chicken model","volume":"25","author":"Dhurandhar","year":"2001","journal-title":"Int. J. Obes. Relat. Metab. Disord."},{"key":"B16","doi-asserted-by":"publisher","first-page":"3155","DOI":"10.1093\/jn\/131.10.3155","article-title":"Human adenovirus ad-36 promotes weight gain in male rhesus and marmoset monkeys","volume":"132","author":"Dhurandhar","year":"2002","journal-title":"J. Nutr."},{"key":"B17","doi-asserted-by":"publisher","first-page":"3648","DOI":"10.1210\/en.2011-0225","article-title":"PPARgamma-independent increase in glucose uptake and adiponectin abundance in fat cells","volume":"152","author":"Dubuisson","year":"2011","journal-title":"Endocrinology"},{"key":"B18","doi-asserted-by":"publisher","first-page":"S13","DOI":"10.1038\/sj.ijo.0802907","article-title":"Regulation of PPARgamma activity during adipogenesis","author":"Farmer","year":"2005","journal-title":"Int. J. Obes. (Lond)"},{"key":"B19","doi-asserted-by":"publisher","first-page":"663","DOI":"10.1016\/j.chom.2014.10.004","article-title":"Adenovirus small E1A employs the lysine acetylases p300\/CBP and tumor suppressor Rb to repress select host genes and promote productive virus infection","volume":"16","author":"Ferrari","year":"2014","journal-title":"Cell Host Microbe"},{"key":"B20","doi-asserted-by":"publisher","first-page":"710","DOI":"10.1038\/sj.onc.1206151","article-title":"Selective PDZ protein-dependent stimulation of phosphatidylinositol 3-kinase by the adenovirus E4-ORF1 oncoprotein","volume":"22","author":"Frese","year":"2003","journal-title":"Oncogene"},{"key":"B21","doi-asserted-by":"publisher","first-page":"762","DOI":"10.1038\/nature07823","article-title":"C-myc suppression of miR-23a\/b enhances mitochondrial glutaminase expression and glutamine metabolism","volume":"458","author":"Gao","year":"2009","journal-title":"Nature"},{"key":"B22","doi-asserted-by":"publisher","first-page":"220","DOI":"10.1038\/oby.2009.452","article-title":"Lack of evidence for the role of human adenovirus-36 in obesity in a European cohort","volume":"19","author":"Goossens","year":"2011","journal-title":"Obes. (Silver Spring)"},{"key":"B23","doi-asserted-by":"publisher","first-page":"783","DOI":"10.1152\/physrev.1998.78.3.783","article-title":"Understanding adipocyte differentiation","volume":"78","author":"Gregoire","year":"1998","journal-title":"Physiol. Rev."},{"key":"B24","doi-asserted-by":"publisher","first-page":"1062","DOI":"10.1101\/gad.3.7.1062","article-title":"An adenovirus early region 4 gene product is required for induction of the infection-specific form of cellular E2F activity","volume":"3","author":"Hardy","year":"1989","journal-title":"Genes Dev."},{"key":"B25","doi-asserted-by":"publisher","first-page":"2732","DOI":"10.1128\/jvi.64.6.2732-2742.1990","article-title":"The adenovirus L4 100-kilodalton protein is necessary for efficient translation of viral late mRNA species","volume":"64","author":"Hayes","year":"1990","journal-title":"J. Virol."},{"key":"B26","first-page":"a032607","article-title":"Principles of Translational Control","volume-title":"Cold Spring Harb Perspect Biol","author":"Hershey","year":"2019"},{"key":"B27","first-page":"901","article-title":"Adenovirus inhibition of cellular protein synthesis and preferential translation of viral mRNAs","volume-title":"Translational control of gene expression","author":"Hershey","year":"2000"},{"key":"B28","doi-asserted-by":"publisher","first-page":"3411","DOI":"10.1128\/JVI.00033-16","article-title":"Morphological, biochemical, and functional study of viral replication compartments isolated from adenovirus-infected cells","volume":"90","author":"Hidalgo","year":"2016","journal-title":"J. Virol."},{"key":"B29","doi-asserted-by":"publisher","first-page":"e02062","DOI":"10.1128\/jvi.02062-21","article-title":"E1B-55K is a phosphorylation-dependent transcriptional and posttranscriptional regulator of viral gene expression in human adenovirus C5 infection","volume":"96","author":"Hidalgo","year":"2022","journal-title":"J. Virol."},{"key":"B30","doi-asserted-by":"publisher","first-page":"1699","DOI":"10.1101\/gad.3.11.1699","article-title":"The adenovirus early region 4 open reading frame 6\/7 protein regulates the DNA binding activity of the cellular transcription factor, E2F, through a direct complex","volume":"3","author":"Huang","year":"1989","journal-title":"Genes Dev."},{"key":"B31","doi-asserted-by":"publisher","first-page":"231","DOI":"10.1016\/S2213-8587(19)30026-9","article-title":"The obesity transition: stages of the global epidemic","volume":"7","author":"Jaacks","year":"2019","journal-title":"Lancet Diabetes Endocrinol."},{"key":"B32","doi-asserted-by":"publisher","first-page":"11544","DOI":"10.1128\/JVI.05410-11","article-title":"Emerging theme: cellular PDZ proteins as common targets of pathogenic viruses","volume":"85","author":"Javier","year":"2011","journal-title":"J. Virol."},{"key":"B33","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1007\/s11010-016-2912-x","article-title":"Regulation of PPAR? and CIDEC expression by adenovirus 36 in adipocyte differentiation","volume":"428","author":"Jiao","year":"2017","journal-title":"Mol. Cell. Biochem."},{"key":"B34","doi-asserted-by":"publisher","first-page":"10774","DOI":"10.1128\/JVI.01463-15","article-title":"Adenovirus E4-ORF1 dysregulates epidermal growth factor and Insulin\/Insulin-like growth factor receptors to mediate constitutive myc expression","volume":"89","author":"Kong","year":"2015","journal-title":"J. Virol."},{"key":"B35","doi-asserted-by":"publisher","first-page":"E779","DOI":"10.1152\/ajpendo.00703.2010","article-title":"Template to improve glycemic control without reducing adiposity or dietary fat","volume":"300","author":"Krishnapuram","year":"2011","journal-title":"Am. J. Physiol. Endocrinol. Metab."},{"key":"B36","doi-asserted-by":"publisher","first-page":"653","DOI":"10.1016\/j.molmet.2015.07.004","article-title":"E4orf1 induction in adipose tissue promotes insulin-independent signaling in the adipocyte","volume":"4","author":"Kusminski","year":"2015","journal-title":"Mol. Metab."},{"key":"B37","doi-asserted-by":"publisher","first-page":"12532","DOI":"10.1073\/pnas.211416898","article-title":"C\/EBPalpha is required for differentiation of white, but not brown, adipose tissue","volume":"98","author":"Linhart","year":"2001","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"B38","doi-asserted-by":"publisher","first-page":"6225","DOI":"10.1128\/MCB.25.14.6225-6234.2005","article-title":"Myc stimulates nuclearly encoded mitochondrial genes and mitochondrial biogenesis","volume":"25","author":"Li","year":"2005","journal-title":"Mol. Cell Biol."},{"key":"B39","doi-asserted-by":"publisher","first-page":"1396","DOI":"10.1128\/jvi.70.3.1396-1405.1996","article-title":"Properties of the adenovirus IVa2 gene product, an effector of late-phase-dependent activation of the major late promoter","volume":"70","author":"Lutz","year":"1996","journal-title":"J. Virol."},{"key":"B40","doi-asserted-by":"publisher","first-page":"5102","DOI":"10.1128\/jvi.71.7.5102-5109.1997","article-title":"The product of the adenovirus intermediate gene IX is a transcriptional activator","volume":"71","author":"Lutz","year":"1997","journal-title":"J. Virol."},{"key":"B41","doi-asserted-by":"publisher","first-page":"345","DOI":"10.1146\/annurev.bi.64.070195.002021","article-title":"Transcriptional regulation of gene expression during adipocyte differentiation","volume":"64","author":"MacDougald","year":"1995","journal-title":"Annu. Rev. Biochem."},{"key":"B42","doi-asserted-by":"publisher","first-page":"251","DOI":"10.1099\/0022-1317-10-3-251","article-title":"Studies on lipid metabolism in cells infected with adenovirus","volume":"10","author":"McIntosh","year":"1971","journal-title":"J. Gen. Virol"},{"key":"B43","doi-asserted-by":"publisher","first-page":"250","DOI":"10.1159\/000468763","article-title":"Control of translation in adenovirus-infected cells","volume":"44","author":"Mathews","year":"1990","journal-title":"Enzyme"},{"key":"B44","doi-asserted-by":"publisher","first-page":"289","DOI":"10.1111\/j.1467-789X.2009.00677.x","article-title":"Viral obesity: Fact or fiction","volume":"11","author":"Mitra","year":"2010","journal-title":"Obes. Rev."},{"key":"B45","doi-asserted-by":"publisher","first-page":"124","DOI":"10.3390\/ijms17010124","article-title":"Molecular regulation of adipogenesis and potential anti-adipogenic bioactive molecules","volume":"17","author":"Moseti","year":"2016","journal-title":"Int. J. Mol. Sci."},{"key":"B46","doi-asserted-by":"publisher","first-page":"e002096","DOI":"10.1136\/bmjdrc-2020-002096","article-title":"Antidiabetic E4orf1 protein prevents hepatic steatosis and reduces markers of aging-related cellular damage in high fat fed older mice","volume":"9","author":"Mostofinejad","year":"2021","journal-title":"BMJ Open Diabetes Res. Care"},{"key":"B47","doi-asserted-by":"publisher","first-page":"1179","DOI":"10.1038\/nbt.1500","article-title":"Systems-level metabolic flux profiling identifies fatty acid synthesis as a target for antiviral therapy","volume":"26","author":"Munger","year":"2008","journal-title":"Nat. Biotechnol."},{"key":"B48","doi-asserted-by":"publisher","first-page":"281","DOI":"10.1038\/ijo.2011.102","article-title":"Association of human adenovirus-36 in overweight Korean adults","volume":"36","author":"Na","year":"2012","journal-title":"Int. J. Obes. (Lond)"},{"key":"B49","doi-asserted-by":"publisher","first-page":"9543","DOI":"10.1073\/pnas.87.24.9543","article-title":"Adenovirus early region 4 stimulates mRNA accumulation via 5\u2019 introns","volume":"87","author":"Nordqvist","year":"1990","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"B50","doi-asserted-by":"publisher","first-page":"437","DOI":"10.1128\/MCB.14.1.437","article-title":"Human adenovirus encodes two proteins which have opposite effects on accumulation of alternatively spliced mRNAs","volume":"14","author":"Nordqvist","year":"1994","journal-title":"Mol. Cell Biol."},{"key":"B51","doi-asserted-by":"publisher","first-page":"21797","DOI":"10.1074\/jbc.C000023200","article-title":"Deregulation of glucose transporter 1 and glycolytic gene expression by c-myc","volume":"275","author":"Osthus","year":"2000","journal-title":"J. Biol. Chem."},{"key":"B52","doi-asserted-by":"publisher","first-page":"61","DOI":"10.1016\/S1043-4526(06)52002-9","article-title":"Infectobesity: obesity of infectious origin","volume":"52","author":"Pasarica","year":"2007","journal-title":"Adv. Food Nutr. Res."},{"key":"B53","doi-asserted-by":"publisher","first-page":"969","DOI":"10.1634\/stemcells.2007-0868","article-title":"Adipogenic human adenovirus ad-36 induces commitment, differentiation, and lipid accumulation in human adipose-derived stem cells","volume":"26","author":"Pasarica","year":"2008","journal-title":"Stem Cells"},{"key":"B54","doi-asserted-by":"publisher","first-page":"1905","DOI":"10.1038\/oby.2006.222","article-title":"Human adenovirus 36 induces adiposity, increases insulin sensitivity, and alters hypothalamic monoamines in rats","volume":"14","author":"Pasarica","year":"2006","journal-title":"Obesity (Silver Spring)"},{"key":"B55","doi-asserted-by":"publisher","first-page":"470","DOI":"10.1128\/mcb.6.2.470-476.1986","article-title":"The adenovirus E1B-55K transforming polypeptide modulates transport or cytoplasmic stabilization of viral and host cell mRNAs","volume":"6","author":"Pilder","year":"1986","journal-title":"Mol. Cell Biol."},{"key":"B56","doi-asserted-by":"publisher","first-page":"141","DOI":"10.3390\/v11020141","article-title":"Metabolic reprogramming of the host cell by human adenovirus infection","volume":"11","author":"Prusinkiewicz","year":"2019","journal-title":"Viruses"},{"key":"B57","doi-asserted-by":"publisher","first-page":"610","DOI":"10.3390\/v12060610","article-title":"Differential effects of human adenovirus E1A protein isoforms on aerobic glycolysis in A549 human lung epithelial cells","volume":"12","author":"Prusinkiewicz","year":"2020","journal-title":"Viruses"},{"key":"B58","doi-asserted-by":"publisher","first-page":"78","DOI":"10.1038\/sj.ijo.0803358","article-title":"Viral mRNA expression but not DNA replication is required for lipogenic effect of human adenovirus ad-36 in preadipocytes","volume":"31","author":"Rathod","year":"2007","journal-title":"Int. J. Obes. (Lond)"},{"key":"B59","doi-asserted-by":"publisher","first-page":"397","DOI":"10.1038\/sj.ijo.0803748","article-title":"Human adenovirus ad-36 induces adipogenesis via its E4 orf-1 gene","volume":"32","author":"Rogers","year":"2008","journal-title":"Int. J. Obes. (Lond)"},{"key":"B60","doi-asserted-by":"publisher","first-page":"2321","DOI":"10.2337\/db07-1311","article-title":"Metabolically favorable remodeling of human adipose tissue by human adenovirus type 36","volume":"57","author":"Rogers","year":"2008","journal-title":"Diabetes"},{"key":"B61","doi-asserted-by":"publisher","first-page":"247","DOI":"10.1007\/s10552-017-0869-z","article-title":"Energy balance and obesity: what are the main drivers","volume":"28","author":"Romieu","year":"2017","journal-title":"Cancer Causes Control"},{"key":"B62","doi-asserted-by":"publisher","first-page":"22","DOI":"10.1101\/gad.948702","article-title":"C\/EBPalpha induces adipogenesis through PPARgamma: a unified pathway","volume":"16","author":"Rosen","year":"2002","journal-title":"Genes Dev."},{"key":"B63","doi-asserted-by":"publisher","first-page":"4651","DOI":"10.1128\/MCB.24.11.4651-4663.2004","article-title":"Temporal recruitment of transcription factors and SWI\/SNF chromatin-remodeling enzymes during adipogenic induction of the peroxisome proliferator-activated receptor gamma nuclear hormone receptor","volume":"24","author":"Salma","year":"2004","journal-title":"Mol. Cell Biol."},{"key":"B64","doi-asserted-by":"publisher","first-page":"319","DOI":"10.1016\/0042-6822(91)90498-Z","article-title":"The metabolism of host RNAs in cells infected by an adenovirus E4 mutant","volume":"181","author":"Sandler","year":"1991","journal-title":"Virology"},{"key":"B65","doi-asserted-by":"publisher","first-page":"845","DOI":"10.1073\/pnas.79.3.845","article-title":"Coupling of growth arrest and differentiation at a distinct state in the G1 phase of the cell cycle: GD","volume":"79","author":"Scott","year":"1982","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"B66","doi-asserted-by":"publisher","first-page":"895","DOI":"10.1002\/oby.20533","article-title":"Serological data analyses show that adenovirus 36 infection is associated with obesity: a meta-analysis involving 5739 subjects","volume":"22","author":"Shang","year":"2014","journal-title":"Obes. (Silver Spring)"},{"key":"B67","doi-asserted-by":"publisher","first-page":"47","DOI":"10.1016\/S0065-230X(08)60995-1","article-title":"Transcriptional and transforming activities of the adenovirus E1A proteins","volume":"57","author":"Shenk","year":"1991","journal-title":"Adv. Cancer Res."},{"key":"B68","doi-asserted-by":"publisher","first-page":"6658","DOI":"10.1073\/pnas.94.13.6658","article-title":"C-myc transactivation of LDH-a: Implications for tumor metabolism and growth","volume":"94","author":"Shim","year":"1997","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"B69","doi-asserted-by":"publisher","first-page":"7432","DOI":"10.1093\/emboj\/16.24.7432","article-title":"Defective adipocyte differentiation in mice lacking the C\/EBPbeta and\/or C\/EBPdelta gene","volume":"16","author":"Tanaka","year":"1997","journal-title":"EMBO J."},{"key":"B70","doi-asserted-by":"publisher","DOI":"10.1016\/j.tvr.2021.200225","article-title":"Almost famous: Human adenoviruses (and what they have taught us about cancer)","volume":"12","author":"Tessier","year":"2021","journal-title":"Tumour Virus Res."},{"key":"B71","doi-asserted-by":"publisher","first-page":"694","DOI":"10.1016\/j.cmet.2014.03.009","article-title":"Adenovirus E4ORF1-induced MYC activation promotes host cell anabolic glucose metabolism and virus replication","volume":"19","author":"Thai","year":"2014","journal-title":"Cell Metab."},{"key":"B72","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms9873","article-title":"MYC-induced reprogramming of glutamine catabolism supports optimal virus replication","volume":"6","author":"Thai","year":"2015","journal-title":"Nat. Commun."},{"key":"B73","doi-asserted-by":"publisher","first-page":"4450","DOI":"10.1128\/jvi.68.7.4450-4457.1994","article-title":"The product of the adenovirus intermediate gene IVa2 is a transcriptional activator of the major late promoter","volume":"68","author":"Tribouley","year":"1994","journal-title":"J. Virol."},{"key":"B74","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0204522","article-title":"Time-resolved proteomics of adenovirus infected cells","volume":"13","author":"Vald\u00e9s","year":"2018","journal-title":"PloS One"},{"key":"B75","doi-asserted-by":"publisher","first-page":"770","DOI":"10.1038\/oby.2004.93","article-title":"A human adenovirus enhances preadipocyte differentiation","volume":"12","author":"Vangipuram","year":"2004","journal-title":"Obes. Res."},{"key":"B76","doi-asserted-by":"publisher","first-page":"87","DOI":"10.1038\/sj.ijo.0803366","article-title":"Adipogenic human adenovirus-36 reduces leptin expression and secretion and increases glucose uptake by fat cells","volume":"31","author":"Vangipuram","year":"2007","journal-title":"Int. J. Obes. (Lond)"},{"key":"B77","doi-asserted-by":"publisher","first-page":"379","DOI":"10.1002\/rmv.1852","article-title":"Role of adenoviruses in obesity","volume":"25","author":"Voss","year":"2015","journal-title":"Rev. Med. Virol."},{"key":"B78","doi-asserted-by":"publisher","first-page":"1805","DOI":"10.2337\/db07-1313","article-title":"Human adenovirus type 36 enhances glucose uptake in diabetic and nondiabetic human skeletal muscle cells independent of insulin signaling","volume":"57","author":"Wang","year":"2008","journal-title":"Diabetes"},{"key":"B79","doi-asserted-by":"publisher","first-page":"R190","DOI":"10.1152\/ajpregu.00479.2005","article-title":"Adipogenic potential of multiple human adenoviruses in vivo and in vitro in animals","volume":"290","author":"Whigham","year":"2006","journal-title":"Am J Physiol. Regul. Integr. Comp. Physiol."},{"key":"B80","doi-asserted-by":"publisher","first-page":"115","DOI":"10.1002\/jcp.1041120117","article-title":"Topography of the predifferentiation GD growth arrest state relative to other growth arrest states in the G1 phase of the cell cycle","volume":"112","author":"Wille","year":"1982","journal-title":"J. Cell Physiol."},{"key":"B81","doi-asserted-by":"publisher","first-page":"151","DOI":"10.1016\/S1097-2765(00)80306-8","article-title":"Cross-regulation of C\/EBP alpha and PPAR gamma controls the transcriptional pathway of adipogenesis and insulin sensitivity","volume":"3","author":"Wu","year":"1999","journal-title":"Mol. Cell"},{"key":"B82","doi-asserted-by":"publisher","DOI":"10.1097\/MD.0000000000002357","article-title":"Human adenovirus 36 infection increased the risk of obesity: A meta-analysis update","volume":"94","author":"Xu","year":"2015","journal-title":"Med. (Baltimore)"},{"key":"B83","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0042031","article-title":"Association of adenovirus 36 infection with obesity and metabolic markers in humans: A meta-analysis of observational studies","volume":"7","author":"Yamada","year":"2012","journal-title":"PloS One"},{"key":"B84","doi-asserted-by":"publisher","first-page":"168","DOI":"10.1101\/gad.9.2.168","article-title":"Cascade regulation of terminal adipocyte differentiation by three members of the C\/EBP family of leucine zipper proteins","volume":"9","author":"Yeh","year":"1995","journal-title":"Genes Dev."}],"container-title":["Frontiers in Cellular and Infection Microbiology"],"original-title":[],"link":[{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fcimb.2022.1016200\/full","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,9,27]],"date-time":"2022-09-27T05:32:47Z","timestamp":1664256767000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fcimb.2022.1016200\/full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,9,27]]},"references-count":84,"alternative-id":["10.3389\/fcimb.2022.1016200"],"URL":"https:\/\/doi.org\/10.3389\/fcimb.2022.1016200","relation":{},"ISSN":["2235-2988"],"issn-type":[{"type":"electronic","value":"2235-2988"}],"subject":[],"published":{"date-parts":[[2022,9,27]]},"article-number":"1016200"}}