{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T04:11:21Z","timestamp":1787026281254,"version":"build-2736575974"},"reference-count":54,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2022,11,14]],"date-time":"2022-11-14T00:00:00Z","timestamp":1668384000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Cell. Infect. Microbiol."],"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Radiation-induced skin injury (RISI) is still the most common and severe side effect of radiotherapy. The role of the skin\u2019s microbial barrier in the pathogenesis and progression of RISI needs to be fully investigated.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>This study aimed to explore the alterations in and functions of the skin microbiota in RISI. We applied the unculturable approach to characterize the cutaneous microbiomes of a radiation-induced animal model by sequencing the V1\u2013V3 regions of the 16S ribosomal RNA (rRNA) gene. Combined with the downloaded clinical data of patients, a comprehensive analysis was performed to identify potential radioprotective species and metabolic pathways.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      There were no significant differences in the alpha diversity indices (Sobs, Shannon, Simpson, Ace, and Chao) between the acute radiation injury and control groups. Phylum-level analysis of the RISI microbiomes exhibited significant predominance of Firmicutes (mean abundance\u00a0=\u00a067%, corrected\n                      <jats:italic>p<\/jats:italic>\n                      \u00a0=\u00a00.0035). The high abundance of Firmicutes was significantly associated with rapid healing of RISI (average relative abundance\u00a0=\u00a052%; Kruskal\u2013Wallis:\n                      <jats:italic>p<\/jats:italic>\n                      \u00a0=\u00a05.7E\u22124). Among its members,\n                      <jats:italic>Streptococcus<\/jats:italic>\n                      ,\n                      <jats:italic>Staphylococcus<\/jats:italic>\n                      ,\n                      <jats:italic>Acetivibrio ethanolgignens group<\/jats:italic>\n                      ,\n                      <jats:italic>Peptostreptococcus<\/jats:italic>\n                      ,\n                      <jats:italic>Anaerofilum<\/jats:italic>\n                      , and\n                      <jats:italic>UCG-002<\/jats:italic>\n                      [linear discriminant analysis (LDA)\u00a0&amp;gt;\u00a03,\n                      <jats:italic>p<\/jats:italic>\n                      \u00a0&amp;lt;\u00a00.05] were identified as the core genera of Firmicutes. In addition, Lachnosiraceae and\n                      <jats:italic>Lactobacillus<\/jats:italic>\n                      occupied an important position in the interaction network (\n                      <jats:italic>r<\/jats:italic>\n                      \u00a0&amp;gt;\u00a00.6,\n                      <jats:italic>p<\/jats:italic>\n                      \u00a0&amp;lt;\u00a00.05). The differential metabolic pathways of RISI were mainly associated with carbohydrate metabolism (butanoate and propanoate metabolism), amino acid metabolism (tryptophan and histidine metabolism), energy metabolism, and lipid metabolism (fatty acid degradation and biosynthesis).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      This study provides new insights into the potential mechanism and skin microbial changes in the progression of RISI. The overwhelming predominance of members of Firmicutes, including Streptococcaceae, Staphylococcaceae, Lachnospiraceae, and\n                      <jats:italic>Lactobacillus<\/jats:italic>\n                      , is potentially related to rapid healing of RISI. The microbiota\u2013metabolite axis plays a critical role in RISI and provides promising therapeutic targets for the treatment of adverse side effects.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.3389\/fcimb.2022.1029592","type":"journal-article","created":{"date-parts":[[2022,11,14]],"date-time":"2022-11-14T00:17:01Z","timestamp":1668385021000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":16,"title":["Exploring the alterations and function of skin microbiome mediated by ionizing radiation injury"],"prefix":"10.3389","volume":"12","author":[{"given":"Biao","family":"Huang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lu","family":"An","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenxing","family":"Su","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tao","family":"Yan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haifang","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dao-Jiang","family":"Yu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2022,11,14]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"1174","DOI":"10.1136\/gutjnl-2020-323071","article-title":"Gut microbiota-derived metabolites as central regulators in metabolic disorders","volume":"70","author":"Agus","year":"2021","journal-title":"Gut"},{"key":"B2","doi-asserted-by":"publisher","first-page":"390","DOI":"10.1007\/s00018-022-04394-z","article-title":"-omics potential of in vitro skin models for radiation exposure","volume":"79","author":"Akh","year":"2022","journal-title":"Cell. 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