{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,11]],"date-time":"2026-10-11T05:51:03Z","timestamp":1791697863305,"version":"4.3.4"},"reference-count":54,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2022,11,18]],"date-time":"2022-11-18T00:00:00Z","timestamp":1668729600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Cell. Infect. Microbiol."],"abstract":"<jats:sec><jats:title>Introduction<\/jats:title><jats:p><jats:italic>Pseudomonas aeruginosa<\/jats:italic> is a common clinical opportunistic pathogen. Antibiotic resistance of <jats:italic>P. aeruginosa<\/jats:italic> is frequent, and it affects the clinical curative effect and leads to recurrent infections, disease progression, and difficult treatment, especially in cystic fibrosis patients. The drug-resistance mechanism of <jats:italic>P. aeruginosa<\/jats:italic> is complex, and biofilms play an important role. Given the widespread antibiotic resistance of <jats:italic>P. aeruginosa<\/jats:italic>, the discovery of a drug that can prevent or eradicate biofilm formation is imperative. Daphnetin (DAP), a coumarin derivative, is a safe, non-toxic, natural compound with antibacterial and anti-biofilm properties. Herein, this study highlights the bacterial motility effects, antibacterial effect, pyocyanin production, and anti-biofilm potential of DAP against <jats:italic>P. aeruginosa<\/jats:italic>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>In this study, the minimal inhibitory concentration of DAP against <jats:italic>P. aeruginosa<\/jats:italic> was determined using the microdilution method. The antibiofilm activity of DAP against <jats:italic>P. aeruginosa<\/jats:italic> was determined using crystal violet staining, colony-forming unit enumeration, and scanning electron microscopy. The effect of DAP on <jats:italic>P. aeruginosa<\/jats:italic> motility was detected using the swimming, swarming, and twitching agar plates to measure the diameter of the concentric area.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>We found that DAP at concentrations of 0.445\u20131.781 mg\/mL and 0.89\u20131.781 mg\/mL can effectively inhibit biofilm formation and eradicate the formed biofilm of <jats:italic>P. aeruginosa<\/jats:italic>, respectively. DAP reduced pyocyanin production and inhibited bacterial motility of <jats:italic>P. aeruginosa<\/jats:italic>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Discussion<\/jats:title><jats:p>In conclusion, our results support the conclusion that DAP can effectively eradicate formed biofilm and inhibit biofilm formation, bacterial motility, and pyocyanin production of <jats:italic>P. aeruginosa<\/jats:italic> and may represent a natural anti-biofilm therapeutic agent.<\/jats:p><\/jats:sec>","DOI":"10.3389\/fcimb.2022.1033540","type":"journal-article","created":{"date-parts":[[2022,11,18]],"date-time":"2022-11-18T05:06:40Z","timestamp":1668748000000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":24,"title":["Effects of daphnetin on biofilm formation and motility of pseudomonas aeruginosa"],"prefix":"10.3389","volume":"12","author":[{"given":"Zuoji","family":"Ye","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liumei","family":"Ye","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dingbin","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shunsheng","family":"Lin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wusheng","family":"Deng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinhua","family":"Liang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinlong","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qingjun","family":"Wei","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ke","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2022,11,18]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"350","DOI":"10.1016\/j.drudis.2018.07.003","article-title":"Updates on the pathogenicity status of pseudomonas aeruginosa","volume":"24","author":"Azam","year":"2019","journal-title":"Drug Discovery Today"},{"key":"B2","doi-asserted-by":"publisher","first-page":"42","DOI":"10.3390\/antibiotics10010042","article-title":"It's not easy being green: A narrative review on the microbiology, virulence and therapeutic prospects of multidrug-resistant pseudomonas aeruginosa","volume":"10","author":"Behzadi","year":"2021","journal-title":"Antibiotics (Basel)"},{"key":"B3","doi-asserted-by":"publisher","first-page":"3761","DOI":"10.1038\/s41596-021-00572-8","article-title":"Testing physiologically relevant conditions in minimal inhibitory concentration assays","volume":"16","author":"Belanger","year":"2021","journal-title":"Nat. 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