{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,11]],"date-time":"2026-07-11T03:29:14Z","timestamp":1783740554698,"version":"3.55.0"},"reference-count":25,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2023,1,10]],"date-time":"2023-01-10T00:00:00Z","timestamp":1673308800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Cell. Infect. Microbiol."],"abstract":"<jats:sec><jats:title>Introduction<\/jats:title><jats:p>Yangambin and epi-yangambin are the main lignans found in Louro-de-Cheiro [<jats:italic>Ocotea fasciculata<\/jats:italic> (Nees) Mez], a tree native to the Atlantic forests of northeastern Brazil whose leaves and bark are widely used in folk medicine. The present study investigated the leishmanicidal and immunomodulatory effects of both lignans in <jats:italic>in vitro<\/jats:italic> models of infection by <jats:italic>Leishmania amazonensis<\/jats:italic> or <jats:italic>Leishmania braziliensis<\/jats:italic>, both etiological agents of Cutaneous Leishmaniasis in Brazil.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Bone marrow-derived mouse macrophages were infected with <jats:italic>L.\u00a0amazonensis<\/jats:italic> or <jats:italic>L. braziliensis<\/jats:italic> and then treated for 48\u00a0h at varying concentrations of yangambin or epi-yangambin.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Yangambin and epi-yangambin were found to reduce the intracellular viability of either <jats:italic>Leishmania<\/jats:italic> species in a concentration-dependent manner, with respective IC<jats:sub>50<\/jats:sub> values of: 43.9 \u00b1 5 and 22.6 \u00b1 4.9 \u00b5M for <jats:italic>L. amazonensis<\/jats:italic>, compared to IC<jats:sub>50<\/jats:sub> values of 76 \u00b1 17 and 74.4 \u00b1 9.8 \u00b5M for <jats:italic>L. braziliensis<\/jats:italic>. In this context, epi-yangambin proved more selective and effective against <jats:italic>in vitro<\/jats:italic> infection by <jats:italic>L. amazonensis<\/jats:italic>. However, both lignans were found to distinctly modulate the production of inflammatory mediators and other cytokines by macrophages infected by either of the <jats:italic>Leishmania<\/jats:italic> species evaluated. While yangambin increased the production of IL-10 by <jats:italic>L. braziliensis-<\/jats:italic>infected macrophages, both compounds were observed to lower the production of NO, PGE<jats:sub>2<\/jats:sub>, IL-6 and TNF-\u03b1 in both <jats:italic>Leishmania<\/jats:italic> species.<\/jats:p><\/jats:sec><jats:sec><jats:title>Discussion<\/jats:title><jats:p>The present results serve to encourage the development of novel studies aimed at screening natural bioactive compounds with the hope of discovering new therapeutic options for the treatment of Cutaneous Leishmaniasis.<\/jats:p><\/jats:sec>","DOI":"10.3389\/fcimb.2022.1045732","type":"journal-article","created":{"date-parts":[[2023,1,10]],"date-time":"2023-01-10T21:42:31Z","timestamp":1673386951000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":6,"title":["In vitro leishmanicidal effect of Yangambin and Epi-yangambin lignans isolated from Ocotea fasciculata (Nees) Mez"],"prefix":"10.3389","volume":"12","author":[{"given":"J\u00e9ssica","family":"Rebou\u00e7as-Silva","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gabriel Farias","family":"Santos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9 Maria Barbosa","family":"Filho","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andresa A.","family":"Berretta","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Franciane","family":"Marquele-Oliveira","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Val\u00e9ria M.","family":"Borges","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2023,1,10]]},"reference":[{"key":"B1","first-page":"7","article-title":"Avalia\u00e7\u00e3o da poss\u00edvel atividade analg\u00e9sica da iangambina obtida de ocotea duckei vatimo","volume":"14","author":"Almeida","year":"1995","journal-title":"Cienc Cult Sa\u00fade"},{"key":"B2","doi-asserted-by":"publisher","first-page":"6863","DOI":"10.3390\/molecules19056863","article-title":"Calcium influx inhibition is involved in the hypotensive and vasorelaxant effects induced by yangambin","volume":"19","author":"Ara\u00fajo","year":"2014","journal-title":"Molecules"},{"key":"B3","doi-asserted-by":"publisher","first-page":"131","DOI":"10.1016\/j.jep.2015.08.001","article-title":"Validation of ethnomedicinal potential of tinospora cordifolia for anticancer and immunomodulatory activities and quantification of bioactive molecules by HPTLC","volume":"175","author":"Bala","year":"2015","journal-title":"J. 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