{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T00:09:06Z","timestamp":1787616546642,"version":"build-2736575974"},"reference-count":25,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2022,12,13]],"date-time":"2022-12-13T00:00:00Z","timestamp":1670889600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002332","name":"Semmelweis Egyetem","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002332","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Cell. Infect. Microbiol."],"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>Characteristics of the blood microbiota among adult patients with community-acquired sepsis are poorly understood. Our aim was to analyze the composition of blood microbiota in adult patients with community-acquired sepsis, and correlate changes with non-septic control patients.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>A prospective observational study was carried out by including adult patients hospitalized for community-acquired sepsis at our center between January and November 2019, by random selection from a pool of eligible patients. Study inclusion was done on the day of sepsis diagnosis. Community acquisition was ascertained by <jats:italic>a priori<\/jats:italic> exclusion criteria; sepsis was defined according to the SEPSIS-3 definitions. Each included patient was matched with non-septic control patients by age and gender in a 1:1 fashion enrolled from the general population. Conventional culturing with BacT\/ALERT system and 16S rRNA microbiota analysis were performed from blood samples taken in a same time from a patient. Abundance data was analyzed by the <jats:italic>CosmosID HUB<\/jats:italic> Microbiome software.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Altogether, 13 hospitalized patients were included, 6\/13 (46.2%) with sepsis and 7\/13 (53.8%) with septic shock at diagnosis. The most prevalent etiopathogen isolated from blood cultures was <jats:italic>Escherichia coli<\/jats:italic>, patients mostly had intraabdominal septic source. At day 28, <jats:italic>all-cause<\/jats:italic> mortality was 15.4% (2\/13). Compared to non-septic control patients, a relative scarcity of <jats:italic>Faecalibacterium<\/jats:italic>, <jats:italic>Blautia, Coprococcus<\/jats:italic> and <jats:italic>Roseburia<\/jats:italic> genera, with an abundance of <jats:italic>Enhydrobacter<\/jats:italic>, <jats:italic>Pseudomonas<\/jats:italic> and <jats:italic>Micrococcus<\/jats:italic> genera was observed among septic patients. Relative differences between septic vs. non-septic patients were more obvious at the phylum level, mainly driven by <jats:italic>Firmicutes<\/jats:italic> (25.7% vs. 63.1%; p&amp;lt;0.01) and <jats:italic>Proteobacteria<\/jats:italic> (36.9% vs. 16.6%; p&amp;lt;0.01). The alpha diversity, quantified by the <jats:italic>Chao1<\/jats:italic> index showed statistically significant difference between septic vs. non-septic patients (126 \u00b1 51 vs. 66 \u00b1 26; p&amp;lt;0.01). The Bray-Curtis beta diversity, reported by principal coordinate analysis of total hit frequencies, revealed 2 potentially separate clusters among septic vs. non-septic patients.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>In adult patients with community-acquired sepsis, specific changes in the composition and abundance of blood microbiota could be detected by 16S rRNA metagenome sequencing, compared to non-septic control patients. Traditional blood culture results only partially correlate with microbiota test results.<\/jats:p><\/jats:sec>","DOI":"10.3389\/fcimb.2022.1067476","type":"journal-article","created":{"date-parts":[[2022,12,13]],"date-time":"2022-12-13T06:42:30Z","timestamp":1670913750000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":17,"title":["Composition and changes of blood microbiota in adult patients with community-acquired sepsis: A pilot study from bench to bedside"],"prefix":"10.3389","volume":"12","author":[{"given":"B\u00e1lint Gergely","family":"Szab\u00f3","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rebeka","family":"Kiss","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N\u00f3ra","family":"Makra","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kinga","family":"P\u00e9nzes","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eszter","family":"Vad","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katalin","family":"Kamotsay","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D\u00f3ra","family":"Szab\u00f3","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eszter","family":"Ostorh\u00e1zi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2022,12,13]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"278","DOI":"10.1186\/s13054-020-02989-1","article-title":"The gut microbiome's role in the development, maintenance, and outcomes of sepsis","volume":"24","author":"Adelman","year":"2020","journal-title":"Crit. 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