{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,29]],"date-time":"2026-10-29T17:13:13Z","timestamp":1793293993853,"version":"4.5.0"},"reference-count":26,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2022,12,2]],"date-time":"2022-12-02T00:00:00Z","timestamp":1669939200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004608","name":"Natural Science Foundation of Jiangsu Province","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004608","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Cell. Infect. Microbiol."],"abstract":"<jats:sec><jats:title>Objective<\/jats:title><jats:p>To evaluate the performance and validate the diagnostic value of a nucleotide matrix-assisted laser desorption time-of-flight mass spectrometry (MALDI-TOF-MS) with the analysis process optimized in identification of mycobacterium species.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>The optimized analysis process was used for mycobacterial identification in the nucleic MALDI-TOF-MS.\u00a0108 samples were used for assessing the performance of nucleic MALDI-TOF-MS, including 25 reference standards, 37 clinical isolates, 37 BALF, and 9 plasmids. The BALF of 38 patients suspected of pulmonary mycobacterial infection was collected for validation. Clinical etiological diagnosis was used as the gold standard to evaluate the diagnostic value of nucleotide MALDI-TOF-MS.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The sensitivity, specificity, and accuracy of the nucleotide MALDI-TOF-MS in mycobacterial identification were 96.91%, 100% and 97.22%, respectively, and the limit of detection for mycobacterium tuberculosis (MTB) was 50 bacteria\/mL. Among 38\u00a0patients suspected of pulmonary mycobacterial infection, 33 were diagnosed with pulmonary tuberculosis infection, and 5 with non-mycobacterial infection. In clinical validation, the positive rates of MALDI-TOF-MS, Xpert MTB\/RIF, culture and AFS in BALF of patients diagnosed with tuberculosis infection were 72.7%, 63.6%, 54.5% and 27.3%, respectively. The sensitivity\/specificity of MALDI-TOF-MS, Xpert, culture and AFS in diagnosing MTB were 72.7%\/100%, 63.6%\/100%, 54.5%\/100%, 27.3%\/100%, with the areas under the curve of 0.864, 0.818, 0.773, and 0.636, respectively.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Optimized nucleotide MALDI-TOF-MS has satisfactory sensitivity, specificity and low LOD in the identification of mycobacteria, which may serve as a potential assay for mycobacterial identification.<\/jats:p><\/jats:sec>","DOI":"10.3389\/fcimb.2022.1079184","type":"journal-article","created":{"date-parts":[[2022,12,2]],"date-time":"2022-12-02T06:25:56Z","timestamp":1669962356000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":24,"title":["Performance evaluation and clinical validation of optimized nucleotide MALDI-TOF-MS for mycobacterial identification"],"prefix":"10.3389","volume":"12","author":[{"given":"Baiying","family":"Li","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chi","family":"Zhu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lifang","family":"Sun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hang","family":"Dong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yaping","family":"Sun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shangzhi","family":"Cao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Libo","family":"Zhen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qi","family":"Qi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Quanquan","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ting","family":"Mo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huijie","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meihua","family":"Qiu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chao","family":"Song","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qingshan","family":"Cai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2022,12,2]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"2569","DOI":"10.1056\/NEJMsr1813907","article-title":"Pathogen genomics in public health","volume":"381","author":"Armstrong","year":"2019","journal-title":"N Engl. J. Med."},{"key":"B2","doi-asserted-by":"publisher","first-page":"663","DOI":"10.1038\/s41564-018-0349-6","article-title":"Analytical and clinical validation of a microbial cell-free DNA sequencing test for infectious disease","volume":"4","author":"Blauwkamp","year":"2019","journal-title":"Nat. Microbiol."},{"key":"B3","doi-asserted-by":"publisher","first-page":"e00237","DOI":"10.1128\/JCM.00237-18","article-title":"Evaluation of the vitek MS v3.0 matrix-assisted laser desorption ionization-time of flight mass spectrometry system for identification of mycobacterium and nocardia species","volume":"56","author":"Body","year":"2018","journal-title":"J. Clin. Microbiol."},{"key":"B4","doi-asserted-by":"publisher","first-page":"338","DOI":"10.1016\/j.jinf.2013.11.012","article-title":"Evaluation of GeneXpert MTB\/RIF for the detection of mycobacterium tuberculosis and resistance to rifampin in clinical specimens","volume":"68","author":"Bunsow","year":"2014","journal-title":"J. Infection"},{"key":"B5","doi-asserted-by":"publisher","first-page":"451","DOI":"10.1016\/S2213-2600(15)00095-8","article-title":"Xpert MTB\/RIF assay for the diagnosis of pulmonary tuberculosis in children: a systematic review and meta-analysis","volume":"3","author":"Detjen","year":"2015","journal-title":"Lancet Respir. Med."},{"key":"B6","doi-asserted-by":"publisher","first-page":"241","DOI":"10.1016\/j.eimc.2020.05.011","article-title":"(Bruker daltonics) for identification of mycobacterium species isolated directly from liquid medium","volume":"39","author":"Fern\u00e1ndez-Esgueva","year":"2021","journal-title":"Enferm Infecc Microbiol. Clin. (Engl Ed)."},{"key":"B7","doi-asserted-by":"publisher","first-page":"S1201","DOI":"10.1016\/j.ijid.2022.03.011","article-title":"The WHO global tuberculosis 2021 report - not so good news and turning the tide back to end TB","volume":"20","author":"Jeremiah","year":"2022","journal-title":"Int. J. Infect. Dis."},{"key":"B8","doi-asserted-by":"publisher","first-page":"102051","DOI":"10.1016\/j.tube.2021.102051","article-title":"Targeted next generation sequencing directly from sputum for comprehensive genetic information on drug resistant mycobacterium tuberculosis","volume":"127","author":"Kambli","year":"2021","journal-title":"Tuberculosis"},{"key":"B9","first-page":"895","article-title":"China Expert consensus group on nucleic acid mass spectrometry application. Chinese expert consensus on application of nucleic acid mass spectrometry","volume":"98","author":"Kang","year":"2018","journal-title":"Natl. Med. J. China"},{"key":"B10","doi-asserted-by":"publisher","first-page":"132","DOI":"10.1186\/s13000-015-0364-3","article-title":"Detection of KRAS, NRAD and BRAF by mass spectrometry: A sensitive, reliable, fast and cost-effective technique","volume":"10","author":"Kriegsmann","year":"2015","journal-title":"Diagn. Pathol."},{"key":"B11","doi-asserted-by":"publisher","first-page":"114","DOI":"10.1038\/s41426-018-0120-3","article-title":"Evaluation of the VITEK MS knowledge base version 3.0 for the identification of clinically relevant mycobacterium species","volume":"7","author":"Luo","year":"2018","journal-title":"Emerg. Microbes Infect."},{"key":"B12","doi-asserted-by":"publisher","first-page":"280 284","DOI":"10.19871\/j.carolcarrollnkiXFCRBZZ.2020.04.015","article-title":"WHO global tuberculosis report: Global and China key data analysis","volume":"5","author":"Ren","year":"2020","journal-title":"Emerging Infect. Dis. electronic J."},{"key":"B13","doi-asserted-by":"publisher","first-page":"2737","DOI":"10.1128\/JCM.01380-15","article-title":"Evaluation of matrix-assisted laser desorption ionization\u2013time of flflight mass spectrometry for identifification of nontuberculous mycobacteria from clinical isolates","volume":"53","author":"Rodr\u00edguez-S\u00e1nchez","year":"2015","journal-title":"J. Clin. Microbiol."},{"key":"B14","doi-asserted-by":"publisher","first-page":"e01548","DOI":"10.1128\/JCM.01548-17","article-title":"Evaluation of two protein extraction protocols based on freezing and mechanical disruption for identifying nontuberculous mycobacteria by matrix-assisted laser desorption ionization-time of flight mass spectrometry from liquid and solid cultures","volume":"56","author":"Rodriguez-Temporal","year":"2018","journal-title":"J. Clin. Microbiol."},{"key":"B15","doi-asserted-by":"publisher","first-page":"e01103","DOI":"10.1128\/JCM.01103-20","article-title":"Evaluation of MALDI biotyper interpretation criteria for accurate identification of nontuberculous mycobacteria","volume":"58","author":"Rodriguez-Temporal","year":"2020","journal-title":"J. Clin. Microbiol."},{"key":"B16","doi-asserted-by":"publisher","first-page":"983","DOI":"10.3969\/j.issn.1000-6621.2021.10.001","article-title":"Standardize the rational use of molecular biological detection technology for early and accurate diagnosis of tuberculosis","volume":"43","author":"Sha","year":"2021","journal-title":"Chin. J. Antituberc."},{"key":"B17","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/j.ijid.2020.06.042","article-title":"CapitalBio mycobacterium real-time polymerase chain reaction detection test: rapid diagnosis of mycobacterium tuberculosis and nontuberculous mycobacterial infection, international journal of infectious diseases","volume":"98","author":"Shen","year":"2020","journal-title":"Int. J. Infect. Dis."},{"key":"B18","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00472-18","article-title":"Development and optimization of metagenomic next-generation sequencing methods for cerebrospinal fluid diagnostics","volume":"56","author":"Simner","year":"2018","journal-title":"J. Clin. Microbiol."},{"key":"B19","doi-asserted-by":"publisher","first-page":"1526","DOI":"10.1093\/jac\/dkt544","article-title":"High-throughput informative single nucleotide polymorphism-based typing of neisse- ria gonorrhoeae using the sequenom MassARRAY iPLEX platform","volume":"69","author":"Trembizki","year":"2014","journal-title":"J. Antimicrob. Chemother."},{"key":"B20","doi-asserted-by":"publisher","first-page":"642","DOI":"10.3969\/j.issn.1671-9638.2018.07.019","article-title":"Diagnosis of tuberculosis WS 288-2017","volume":"17","author":"Wang","year":"2018","journal-title":"Chin. J. Infection Control"},{"key":"B21","doi-asserted-by":"publisher","first-page":"2408","DOI":"10.1056\/NEJMoa1401268","article-title":"Actionable diagnosis of neuroleptospirosis by next-generation sequencing","volume":"370","author":"Wilson","year":"2014","journal-title":"N Engl. J. Med."},{"key":"B22","doi-asserted-by":"publisher","first-page":"96","DOI":"10.1016\/j.tube.2018.03.010","article-title":"Tag array gene chip rapid diagnosis anti-tuberculosis drug resistance in pulmonary tuberculosis-a feasibility study, tuberculosis","volume":"110","author":"Wu","year":"2018","journal-title":"Tuberculosis"},{"key":"B23","doi-asserted-by":"publisher","first-page":"47","DOI":"10.1016\/j.ijid.2022.04.061","article-title":"Prediction of mycobacterium tuberculosis drug resistance by nucleotide MALDI-TOF-MS","volume":"121","author":"Wu","year":"2022","journal-title":"Int. J. Infect. Dis."},{"key":"B24","doi-asserted-by":"publisher","first-page":"75","DOI":"10.1016\/j.tube.2015.11.005","article-title":"Systematic review: Comparison of xpert MTB\/RIF, LAMP and SAT methods for the diagnosis of pulmonary tuberculosis","volume":"96","author":"Yan","year":"2016","journal-title":"Tuberculosis"},{"key":"B25","doi-asserted-by":"crossref","first-page":"779","DOI":"10.1016\/j.cell.2019.07.010","article-title":"Benchmarking metagenomics tools for taxonomic classifification","volume":"178","author":"Ye","year":"2019","journal-title":"Cell"},{"key":"B26","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2022.1021320","article-title":"Clinical value of metagenomic next-generation sequencing by illumina and nanopore for the detection of pathogens in bronchoalveolar lavage flfluid in suspected community-acquired pneumonia patients","volume":"12","author":"Zhang","year":"2022","journal-title":"Front. Cell. Infect. Microbiol."}],"container-title":["Frontiers in Cellular and Infection Microbiology"],"original-title":[],"link":[{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fcimb.2022.1079184\/full","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,12,2]],"date-time":"2022-12-02T06:26:02Z","timestamp":1669962362000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fcimb.2022.1079184\/full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,12,2]]},"references-count":26,"alternative-id":["10.3389\/fcimb.2022.1079184"],"URL":"https:\/\/doi.org\/10.3389\/fcimb.2022.1079184","relation":{},"ISSN":["2235-2988"],"issn-type":[{"value":"2235-2988","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,12,2]]},"article-number":"1079184"}}