{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T13:14:10Z","timestamp":1790342050093,"version":"4.1.0"},"reference-count":53,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2022,2,22]],"date-time":"2022-02-22T00:00:00Z","timestamp":1645488000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000265","name":"Medical Research Council","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000265","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000268","name":"Biotechnology and Biological Sciences Research Council","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000268","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Cell. Infect. Microbiol."],"abstract":"<jats:p>\n                    In the early-diverging protozoan parasite\n                    <jats:italic>Plasmodium<\/jats:italic>\n                    , few telomere-binding proteins have been identified and several are unique.\n                    <jats:italic>Plasmodium<\/jats:italic>\n                    telomeres, like those of most eukaryotes, contain guanine-rich repeats that can form G-quadruplex structures. In model systems, quadruplex-binding drugs can disrupt telomere maintenance and some quadruplex-binding drugs are potent anti-plasmodial agents. Therefore, telomere-interacting and quadruplex-interacting proteins may offer new targets for anti-malarial therapy. Here, we report that\n                    <jats:italic>P. falciparum<\/jats:italic>\n                    GBP2 is such a protein. It was identified\n                    <jats:italic>via<\/jats:italic>\n                    \u2018Proteomics of Isolated Chromatin fragments\u2019, applied here for the first time in\n                    <jats:italic>Plasmodium<\/jats:italic>\n                    .\n                    <jats:italic>In vitro<\/jats:italic>\n                    ,\n                    <jats:italic>Pf<\/jats:italic>\n                    GBP2 binds specifically to G-rich telomere repeats in quadruplex form and it can also bind to G-rich RNA.\n                    <jats:italic>In vivo<\/jats:italic>\n                    ,\n                    <jats:italic>Pf<\/jats:italic>\n                    GBP2 partially colocalises with the known telomeric protein HP1 but is also found in the cytoplasm, probably due to its affinity for RNA. Consistently, its interactome includes numerous RNA-associated proteins.\n                    <jats:italic>Pf<\/jats:italic>\n                    GBP2 is evidently a multifunctional DNA\/RNA-binding factor in\n                    <jats:italic>Plasmodium<\/jats:italic>\n                    .\n                  <\/jats:p>","DOI":"10.3389\/fcimb.2022.782537","type":"journal-article","created":{"date-parts":[[2022,2,22]],"date-time":"2022-02-22T07:35:53Z","timestamp":1645515353000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":15,"title":["Plasmodium falciparum GBP2 Is a Telomere-Associated Protein That Binds to G-Quadruplex DNA and RNA"],"prefix":"10.3389","volume":"12","author":[{"given":"James","family":"Edwards-Smallbone","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anders L.","family":"Jensen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lydia E.","family":"Roberts","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francis Isidore G.","family":"Tota\u00f1es","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarah R.","family":"Hart","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Catherine J.","family":"Merrick","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2022,2,22]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"9950","DOI":"10.1093\/nar\/gkz727","article-title":"Insights Into Telomeric G-Quadruplex DNA Recognition by HMGB1 Protein","volume":"47","author":"Amato","year":"2019","journal-title":"Nucleic Acids Res."},{"key":"B2","doi-asserted-by":"publisher","first-page":"D539","DOI":"10.1093\/nar\/gkn814","article-title":"PlasmoDB: A Functional Genomic Database for Malaria Parasites","volume":"37","author":"Aurrecoechea","year":"2009","journal-title":"Nucleic Acids Res."},{"key":"B3","doi-asserted-by":"publisher","first-page":"17033","DOI":"10.1038\/nmicrobiol.2017.33","article-title":"Malaria Parasites Possess a Telomere Repeat-Binding Protein That Shares Ancestry With Transcription Factor IIIA","volume":"2","author":"Bertschi","year":"2017","journal-title":"Nat. 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