{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T15:52:33Z","timestamp":1753890753257,"version":"3.41.2"},"reference-count":34,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2022,3,25]],"date-time":"2022-03-25T00:00:00Z","timestamp":1648166400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Cell. Infect. Microbiol."],"abstract":"<jats:p><jats:italic>Listeria monocytogenes<\/jats:italic>, as a model organism, is a causative agent of enteric pathogen that causes systemic infection. However, the interaction of <jats:italic>L. monocytogenes<\/jats:italic> and small intestinal epithelium has not been fully elucidated yet. In this study, mice and intestinal organoids were chosen as the models to investigate the influence of <jats:italic>L. monocytogenes<\/jats:italic> infection on the intestinal secretory cells and its differentiation-related pathways. Results confirmed the phenomenon of intestinal damage that <jats:italic>L. monocytogenes<\/jats:italic> infection could lead to villi damage in mice, which was accompanied by the increase of TNF-\u03b1 production in jejunum as well as lipopolysaccharide (LPS) secretion in serum. Moreover, it was demonstrated that <jats:italic>L. monocytogenes<\/jats:italic> infection increased the number of goblet and Paneth cells in mice and intestinal organoids and upregulated the expression of <jats:italic>Muc2<\/jats:italic> and <jats:italic>Lyz<\/jats:italic>. Furthermore, <jats:italic>L. monocytogenes<\/jats:italic> decreased the relative expression of Notch pathway-related genes (<jats:italic>Jag1<\/jats:italic>, <jats:italic>Dll4<\/jats:italic>, <jats:italic>Notch1<\/jats:italic>, and <jats:italic>Hes1<\/jats:italic>) while upregulating the relative expression of <jats:italic>Math1<\/jats:italic> gene in mice and intestinal organoids. This indicated that <jats:italic>L.\u00a0monocytogenes<\/jats:italic> infection caused the inhibition of Notch pathway, which may be the reason for the increased number of goblet and Paneth cells in the intestine. Collectively, these results are expected to provide more information on the mechanism of <jats:italic>L.\u00a0monocytogene<\/jats:italic>s infection in the intestine.<\/jats:p>","DOI":"10.3389\/fcimb.2022.793335","type":"journal-article","created":{"date-parts":[[2022,3,29]],"date-time":"2022-03-29T15:32:04Z","timestamp":1648567924000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":10,"title":["Expansion of Intestinal Secretory Cell Population Induced by Listeria monocytogenes Infection: Accompanied With the Inhibition of NOTCH Pathway"],"prefix":"10.3389","volume":"12","author":[{"given":"Cong","family":"Zhou","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuanyuan","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anthony","family":"Bassey","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jie","family":"Huang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yafang","family":"Zou","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Keping","family":"Ye","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1965","published-online":{"date-parts":[[2022,3,25]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"573","DOI":"10.3390\/ani10040573","article-title":"The Beneficial Effect of Eco-Friendly Green Nanoparticles Using Garcinia Mangostana Peel Extract Against Pathogenicity of Listeria Monocytogenes in Female BALB\/C Mice","volume":"10","author":"Alkhuriji","year":"2020","journal-title":"Animals"},{"key":"B2","doi-asserted-by":"publisher","first-page":"1","DOI":"10.3390\/pathogens7010001","article-title":"Multifaceted Defense Against Listeria Monocytogenes in the Gastro-Intestinal Lumen","volume":"7","author":"Becattini","year":"2018","journal-title":"Pathogens"},{"key":"B3","doi-asserted-by":"publisher","first-page":"356","DOI":"10.1038\/nrmicro2546","article-title":"Paneth Cells, Antimicrobial Peptides and Maintenance of Intestinal Homeostasis","volume":"9","author":"Bevins","year":"2011","journal-title":"Nat. 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