{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T17:54:12Z","timestamp":1791482052175,"version":"4.3.4"},"reference-count":77,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2022,1,28]],"date-time":"2022-01-28T00:00:00Z","timestamp":1643328000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Cell. Infect. Microbiol."],"abstract":"<jats:p>Among the <jats:italic>Ctenocephalides felis felis<\/jats:italic>-borne pathogens, <jats:italic>Bartonella henselae<\/jats:italic>, the main aetiological agent of cat scratch disease (CSD), is of increasing comparative biomedical importance. Despite the importance of <jats:italic>B. henselae<\/jats:italic> as an emergent pathogen, prevention of the diseases caused by this agent in cats, dogs and humans mostly relies on the use of ectoparasiticides. A vaccine targeting both flea fitness and pathogen competence is an attractive choice requiring the identification of flea proteins\/metabolites with a dual effect. Even though recent developments in vector and pathogen -omics have advanced the understanding of the genetic factors and molecular pathways involved at the tick-pathogen interface, leading to discovery of candidate protective antigens, only a few studies have focused on the interaction between fleas and flea-borne pathogens. Taking into account the period of time needed for <jats:italic>B. henselae<\/jats:italic> replication in flea digestive tract, the present study investigated flea-differentially abundant proteins (FDAP) in unfed fleas, fleas fed on uninfected cats, and fleas fed on <jats:italic>B. henselae<\/jats:italic>-infected cats at 24 hours and 9 days after the beginning of blood feeding. Proteomics approaches were designed and implemented to interrogate differentially expressed proteins, so as to gain a better understanding of proteomic changes associated with the initial <jats:italic>B. henselae<\/jats:italic> transmission period (24 hour timepoint) and a subsequent time point 9 days after blood ingestion and flea infection. As a result, serine proteases, ribosomal proteins, proteasome subunit \u03b1-type, juvenile hormone epoxide hydrolase 1, vitellogenin C, allantoinase, phosphoenolpyruvate carboxykinase, succinic semialdehyde dehydrogenase, glycinamide ribotide transformylase, secreted salivary acid phosphatase had high abundance in response of <jats:italic>C. felis<\/jats:italic> blood feeding and\/or infection by <jats:italic>B. henselae<\/jats:italic>. In contrast, high abundance of serpin-1, arginine kinase, ribosomal proteins, peritrophin-like protein, and FS-H\/FSI antigen family member 3 was strongly associated with unfed cat fleas. Findings from this study provide insights into proteomic response of cat fleas to <jats:italic>B.\u00a0henselae<\/jats:italic> infected and uninfected blood meal, as well as <jats:italic>C. felis<\/jats:italic> response to invading <jats:italic>B.\u00a0henselae<\/jats:italic> over an infection time course, thus helping understand the complex interactions between cat fleas and <jats:italic>B. henselae<\/jats:italic> at protein levels.<\/jats:p>","DOI":"10.3389\/fcimb.2022.828082","type":"journal-article","created":{"date-parts":[[2022,1,28]],"date-time":"2022-01-28T06:16:16Z","timestamp":1643350576000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":15,"title":["Using Proteomic Approaches to Unravel the Response of Ctenocephalides felis felis to Blood Feeding and Infection With Bartonella henselae"],"prefix":"10.3389","volume":"12","author":[{"given":"Marcos Rog\u00e9rio","family":"Andr\u00e9","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pradeep","family":"Neupane","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Lappin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brian","family":"Herrin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vicki","family":"Smith","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Taufika Islam","family":"Williams","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leonard","family":"Collins","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongxia","family":"Bai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gabriel Lemes","family":"Jorge","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tiago Santana","family":"Balbuena","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julie","family":"Bradley","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ricardo G.","family":"Maggi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edward B.","family":"Breitschwerdt","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2022,1,28]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"41","DOI":"10.1016\/s0147-9571(96)00025-2","article-title":"Experimental and Natural Infection With Bartonella henselae in Cats","volume":"20","author":"Abbott","year":"1997","journal-title":"Comp. Immunol. Microbiol. Infect. Dis."},{"key":"B2","doi-asserted-by":"publisher","first-page":"102","DOI":"10.1186\/1471-2164-8-102","article-title":"An Insight Into the Sialome of the Oriental Rat Flea, Xenopsylla cheopis (Rots)","volume":"8","author":"Andersen","year":"2007","journal-title":"BMC Genomics"},{"key":"B3","doi-asserted-by":"publisher","DOI":"10.1016\/j.pt.2016.04.009","article-title":"Rickettsia felis: The Complex Journey of an Emergent Human Pathogen","volume":"32","author":"Angelakis","year":"2016","journal-title":"Trends Parasitol."},{"key":"B4","doi-asserted-by":"publisher","first-page":"242","DOI":"10.1186\/1471-2164-10-242","article-title":"Gene Expression During Drosophila melanogaster Egg Development Before and After Reproductive Diapause","volume":"10","author":"Baker","year":"2009","journal-title":"BMC Genomics"},{"key":"B5","doi-asserted-by":"publisher","first-page":"S18","DOI":"10.1016\/j.vetpar.2017.03.010","article-title":"Efficacy and Speed of Kill of a New Spot-on Formulation of Selamectin Plus Sarolaner Against Flea Infestations in Cats","volume":"238","author":"Becskei","year":"2017","journal-title":"Vet. Parasitol."},{"key":"B6","doi-asserted-by":"publisher","first-page":"93","DOI":"10.1016\/s0378-1119(98)00256-x","article-title":"Evolutionary Conservation of a Testes-Specific Proteasome Subunit Gene in Drosophila","volume":"215","author":"Belote","year":"1998","journal-title":"Gene"},{"key":"B7","doi-asserted-by":"publisher","first-page":"e0008688","DOI":"10.1371\/journal.pntd.0008688","article-title":"Transcriptomic Profiling of the Digestive Tract of the Rat Flea, Xenopsylla cheopis, Following Blood Feeding and Infection With Yersinia Pestis","volume":"14","author":"Bland","year":"2020","journal-title":"PloS Negl. Trop. Dis."},{"key":"B8","doi-asserted-by":"publisher","first-page":"7439","DOI":"10.1128\/AEM.02598-13","article-title":"Assessment of Persistence of Bartonella henselae in Ctenocephalides felis","volume":"79","author":"Bouhsira","year":"2013","journal-title":"Appl. Environ. Microbiol."},{"key":"B9","doi-asserted-by":"publisher","first-page":"869","DOI":"10.2460\/javma.236.8.869","article-title":"Evaluation of Topical Application of 10% Imidacloprid-1% Moxidectin to Prevent Bartonella henselae Transmission From Cat Fleas","volume":"236","author":"Bradbury","year":"2010","journal-title":"J. Am. Vet. Med. Assoc."},{"key":"B10","doi-asserted-by":"publisher","first-page":"200","DOI":"10.1002\/arch.10139","article-title":"Isolation, Characterization, and Recombinant Expression of Multiple Serpins From the Cat Flea, Ctenocephalides felis","volume":"55","author":"Brandt","year":"2004","journal-title":"Arch. Insect Biochem. Physiol."},{"key":"B11","doi-asserted-by":"publisher","first-page":"1023","DOI":"10.3390\/pathogens9121023","article-title":"Bartonella\u00a0Associated Cutaneous Lesions (BACL) in People With Neuropsychiatric Symptoms","volume":"9","author":"Breitschwerdt","year":"2020","journal-title":"Pathogens"},{"key":"B12","doi-asserted-by":"publisher","first-page":"428","DOI":"10.1128\/CMR.13.3.428","article-title":"Bartonella Infection in Animals: Carriership, Reservoir Potential, Pathogenicity, and Zoonotic Potential for Human Infection","volume":"13","author":"Breitschwerdt","year":"2000","journal-title":"Clin. Microbiol. Rev."},{"key":"B13","doi-asserted-by":"publisher","first-page":"101","DOI":"10.1007\/s00430-018-0563-0","article-title":"Bartonella Quintana and Bartonella Vinsonii Subsp. Vinsonii Bloodstream Co-Infection in a Girl From North Carolina, USA","volume":"208","author":"Breitschwerdt","year":"2019","journal-title":"Med. Microbiol. Immunol."},{"key":"B14","doi-asserted-by":"publisher","first-page":"8","DOI":"10.1111\/j.1476-4431.2009.00496.x","article-title":"Bartonellosis: An Emerging Infectious Disease of Zoonotic Importance to Animals and Human Beings","volume":"20","author":"Breitschwerdt","year":"2010","journal-title":"Vet. Emerg. Crit. Care"},{"key":"B15","doi-asserted-by":"publisher","first-page":"76","DOI":"10.1016\/j.dci.2019.03.019","article-title":"Immunity of Fleas (Order Siphonaptera)","volume":"98","author":"Brown","year":"2019","journal-title":"Dev. Comp. Immunol."},{"key":"B16","doi-asserted-by":"publisher","first-page":"136","DOI":"10.20506\/rst.19.1.1204","article-title":"Cat-Scratch Disease","volume":"19","author":"Chomel","year":"2000","journal-title":"Rev. Sci. Tech. Off. Int. Epizoot."},{"key":"B17","doi-asserted-by":"publisher","first-page":"1952","DOI":"10.1128\/jcm.34.8.1952-1956.1996","article-title":"Experimental Transmission of Bartonella henselae by the Cat Flea","volume":"34","author":"Chomel","year":"1996","journal-title":"J. Clin. Microbiol."},{"key":"B18","doi-asserted-by":"publisher","first-page":"8","DOI":"10.1186\/1756-3305-7-8","article-title":"Insecticide\/Acaricide Resistance in Fleas and Ticks Infesting Dogs and Cats","volume":"7","author":"Coles","year":"2014","journal-title":"Parasit. Vectors"},{"key":"B19","doi-asserted-by":"publisher","first-page":"43","DOI":"10.1186\/s13071-018-2618-x","article-title":"A Reverse Vaccinology Approach to the Identification and Characterization of Ctenocephalides felis Candidate Protective Antigens for the Control of Cat Flea Infestations","volume":"11","author":"Contreras","year":"2018","journal-title":"Parasit. Vectors"},{"key":"B20","doi-asserted-by":"publisher","first-page":"ftab020","DOI":"10.1093\/femspd\/ftab020","article-title":"Dynamic Gene Expression in Salivary Glands of the Cat Flea During Rickettsia felis Infection","volume":"79","author":"Danchenko","year":"2021","journal-title":"Pathog. Dis."},{"key":"B21","doi-asserted-by":"publisher","first-page":"225","DOI":"10.1093\/oxfordjournals.molbev.a025920","article-title":"Elongation Factor-1 Alpha Occurs as Two Copies in Bees: Implications for Phylogenetic Analysis of EF-1 Alpha Sequences in Insects","volume":"15","author":"Danforth","year":"1998","journal-title":"Mol. Biol. Evol."},{"key":"B22","doi-asserted-by":"publisher","first-page":"114","DOI":"10.3389\/fcimb.2017.00114","article-title":"Tick-Pathogen Interactions and Vector Competence: Identification of Molecular Drivers for Tick-Borne Diseases","volume":"7","author":"de la Fuente","year":"2017","journal-title":"Front. Cell Infect. Microbiol."},{"key":"B23","doi-asserted-by":"publisher","first-page":"5089","DOI":"10.1016\/j.vaccine.2017.07.097","article-title":"Targeting a Global Health Problem: Vaccine Design and Challenges for the Control of Tick-Borne Diseases","volume":"35","author":"de la Fuente","year":"2017","journal-title":"Vaccine"},{"key":"B24","doi-asserted-by":"publisher","first-page":"229","DOI":"10.1111\/j.1365-2583.2009.00978.x","article-title":"Analysis of Rickettsia typhi-Infected and Uninfected Cat Flea (Ctenocephalides felis) Midgut cDNA Libraries: Deciphering Molecular Pathways Involved in Host Response to R. typhi infection","volume":"19","author":"Dreher-Lesnick","year":"2010","journal-title":"Insect Mol. Biol."},{"key":"B25","doi-asserted-by":"publisher","first-page":"273","DOI":"10.1016\/j.mimet.2007.01.010","article-title":"A Combined Approach for the Enhanced Detection and Isolation of Bartonella Species in Dog Blood Samples: Pre-Enrichment Liquid Culture Followed by PCR and Subculture Onto Agar Plates","volume":"69","author":"Duncan","year":"2007","journal-title":"J. Microbiol. Methods"},{"key":"B26","doi-asserted-by":"publisher","first-page":"915","DOI":"10.1603\/0022-2585-39.6.915","article-title":"Studies on the Growth of Bartonella henselae in the Cat Flea","volume":"39","author":"Finkelstein","year":"2002","journal-title":"J. Med. Entomol."},{"key":"B27","doi-asserted-by":"publisher","first-page":"625","DOI":"10.1093\/jmedent\/35.5.625","article-title":"Experimental Infection of Domestic Cats With Bartonella henselae by Inoculation of Ctenocephalides felis (Siphonaptera: Pulicidae) Feces","volume":"35","author":"Foil","year":"1999","journal-title":"J. Med. Entomol."},{"key":"B28","doi-asserted-by":"publisher","first-page":"151","DOI":"10.1186\/1756-3305-5-151","article-title":"Prophylactic Treatment of Flea- Infested Cats With an Imidacloprid\/Flumethrin Collar to Forestall Infection With Dipylidium caninum","volume":"5","author":"Fourie","year":"2012","journal-title":"Parasit. Vectors"},{"key":"B29","doi-asserted-by":"publisher","first-page":"299","DOI":"10.1046\/j.1365-2583.2002.00337.x","article-title":"Analysis of Expressed Sequence Tags From Subtracted and Unsubtracted Ctenocephalides felis Hindgut and Malpighian Tubule cDNA Libraries","volume":"11","author":"Gaines","year":"2002","journal-title":"Insect Mol. Biol."},{"key":"B30","doi-asserted-by":"publisher","first-page":"11","DOI":"10.1046\/j.1365-2583.1999.810011.x","article-title":"Cloning of a Family of Serine Protease Genes From the Cat Flea Ctenocephalides felis","volume":"8","author":"Gaines","year":"1999","journal-title":"Insect Mol. Biol."},{"key":"B31","doi-asserted-by":"publisher","first-page":"203","DOI":"10.1016\/j.ibmb.2003.10.002","article-title":"Insect Allantoinase: cDNA Cloning, Purification, and Characterization of the Native Protein From the Cat Flea, Ctenocephalides felis","volume":"34","author":"Gaines","year":"2004","journal-title":"Insect Biochem. Mol. Biol."},{"key":"B32","doi-asserted-by":"publisher","first-page":"1061","DOI":"10.1016\/s0965-1748(03)00096-1","article-title":"Cloning and Characterization of Five cDNAs Encoding Peritrophin-a Domains From the Cat Flea, Ctenocephalides felis","volume":"33","author":"Gaines","year":"2003","journal-title":"Insect Biochem. Mol. Biol."},{"key":"B33","doi-asserted-by":"publisher","first-page":"16","DOI":"10.1186\/1471-2164-5-74","article-title":"Genome Wide Analysis of Common and Specific Stress Responses in Adult Drosophila melanogaster","volume":"5","author":"Girardot","year":"2004","journal-title":"BMC Genomics"},{"key":"B34","doi-asserted-by":"publisher","first-page":"368","DOI":"10.1186\/s13071-015-0972-5","article-title":"Identification of Genes Associated With Blood Feeding in the Cat Flea, Ctenocephalides felis","volume":"8","author":"Greene","year":"2015","journal-title":"Parasit. Vectors"},{"key":"B35","doi-asserted-by":"publisher","first-page":"1749","DOI":"10.1016\/j.biochi.2010.09.004","article-title":"Protease Inhibitors and Proteolytic Signalling Cascades in Insects","volume":"92","author":"Gubb","year":"2010","journal-title":"Biochimie"},{"key":"B36","doi-asserted-by":"publisher","first-page":"1073","DOI":"10.1016\/j.cvsm.2010.07.009","article-title":"Feline Bartonellosis","volume":"40","author":"Guptill","year":"2010","journal-title":"Vet. Clin. North Am. Small Anim. Pract."},{"key":"B37","doi-asserted-by":"publisher","first-page":"228","DOI":"10.1016\/j.pt.2014.02.007","article-title":"Flea Control Failure? Myths and realities","volume":"30","author":"Halos","year":"2014","journal-title":"Trends Parasitol."},{"key":"B38","doi-asserted-by":"publisher","first-page":"519","DOI":"10.1016\/0022-2836(86)90238-x","article-title":"The Saccharomyces Cerevisiae ADE5,7 Protein is Homologous to Overlapping Drosophila melanogaster Gart Polypeptides","volume":"190","author":"Henikoff","year":"1986","journal-title":"J.\u00a0Mol. Biol."},{"key":"B39","doi-asserted-by":"publisher","first-page":"490","DOI":"10.1093\/jmedent\/33.3.490","article-title":"Acquisition of the Cat Scratch Disease Agent Bartonella henselae by Cat Fleas (Siphonaptera: Pulicidae)","volume":"33","author":"Higgins","year":"1996","journal-title":"J. Med. Entomol."},{"key":"B40","doi-asserted-by":"publisher","first-page":"191","DOI":"10.1002\/arch.10044","article-title":"Cloning, Partial Purification and In Vivo Developmental Profile of Expression of the Juvenile Hormone Epoxide Hydrolase of Ctenocephalides felis","volume":"50","author":"Keiser","year":"2002","journal-title":"Arch. Insect Biochem. Physiol."},{"key":"B41","doi-asserted-by":"publisher","first-page":"1536","DOI":"10.1128\/JCM.37.5.1536-1547.1999","article-title":"Clinical and Pathologic Evaluation of Chronic Bartonella henselae or Bartonella clarridgeiae Infection in Cats","volume":"37","author":"Kordick","year":"1999","journal-title":"J. Clin. Microbiol."},{"key":"B42","doi-asserted-by":"publisher","first-page":"26","DOI":"10.1186\/1756-3305-6-26","article-title":"A Flea and Tick Collar Containing 10% Imidacloprid and 4.5% Flumethrin Prevents Flea Transmission of Bartonella henselae in Cats","volume":"6","author":"Lappin","year":"2013","journal-title":"Parasit. Vectors"},{"key":"B43","doi-asserted-by":"publisher","first-page":"413","DOI":"10.1089\/vbz.2020.2729","article-title":"Schizophrenia And\u00a0Bartonella\u00a0Spp. Infection: A Pilot Case-Control Study","volume":"21","author":"Lashnits","year":"2021","journal-title":"Vector Borne Zoonotic Dis."},{"key":"B44","doi-asserted-by":"publisher","first-page":"734","DOI":"10.1016\/j.pt.2021.05.008","article-title":"Unpacking the Intricacies of Rickettsia\u2013Vector Interactions","volume":"37","author":"Laukaitis","year":"2021","journal-title":"Trends Parasitol."},{"key":"B45","doi-asserted-by":"publisher","first-page":"134","DOI":"10.1152\/physiolgenomics.00262.2005","article-title":"Identification and Function of Hypoxia-Response Genes in Drosophila melanogaster","volume":"25","author":"Liu","year":"2006","journal-title":"Physiol. Genomics"},{"key":"B46","doi-asserted-by":"publisher","first-page":"2651","DOI":"10.1128\/JCM.43.6.2651-2655.2005","article-title":"Novel Chemically Modified Liquid Medium That Will Support the Growth of Seven Bartonella Species","volume":"43","author":"Maggi","year":"2005","journal-title":"J. Clin. Microbiol."},{"key":"B47","doi-asserted-by":"publisher","DOI":"10.1016\/j.mimet.2020.106022","article-title":"Development and Validation of a Droplet Digital PCR Assay for the Detection and Quantification of Bartonella Species Within Human Clinical Samples","volume":"176","author":"Maggi","year":"2020","journal-title":"J. Microbiol. Methods"},{"key":"B48","doi-asserted-by":"publisher","first-page":"1407","DOI":"10.1016\/s0195-5616(99)50135-6","article-title":"Advances in Flea Control","volume":"29","author":"Marsella","year":"1999","journal-title":"Vet. Clin. North Am. Small Anim. Pract."},{"key":"B49","doi-asserted-by":"publisher","first-page":"1532","DOI":"10.1017\/S0031182016001025","article-title":"Reference Gene Selection and RNA Preservation Protocol in the Cat Flea, Ctenocephalides felis, for Gene Expression Studies","volume":"143","author":"McIntosh","year":"2016","journal-title":"Parasitology"},{"key":"B50","doi-asserted-by":"publisher","first-page":"85","DOI":"10.1603\/0022-2585-38.1.85","article-title":"Effects of Juvenile Hormone on Eggs and Adults of the Cat Flea (Siphonaptera: Pulicidae)","volume":"38","author":"Meola","year":"2001","journal-title":"J. Med. Entomol."},{"key":"B51","doi-asserted-by":"publisher","first-page":"1958","DOI":"10.1111\/jvim.15301","article-title":"Evaluation of Cell Culture-Grown Bartonella Antigens in Immunofluorescent Antibody Assays for the Serological Diagnosis of Bartonellosis in Dogs","volume":"32","author":"Neupane","year":"2018","journal-title":"J. Vet. Intern. Med."},{"key":"B52","doi-asserted-by":"publisher","first-page":"3170","DOI":"10.1038\/s41598-019-39508-4","article-title":"Proteasome \u03b25 Subunit Overexpression Improves Proteostasis During Aging and Extends Lifespan in Drosophila melanogaster","volume":"9","author":"Nguyen","year":"2019","journal-title":"Sci. Rep."},{"key":"B53","doi-asserted-by":"publisher","first-page":"e71386","DOI":"10.1371\/journal.pone.0071386","article-title":"Molecular Detection of Rickettsia typhi in Cats and Fleas","volume":"8","author":"Nogueras","year":"2013","journal-title":"PloS One"},{"key":"B54","doi-asserted-by":"publisher","first-page":"1149","DOI":"10.1016\/j.ibmb.2007.07.003","article-title":"A Proteomic Approach to the Identification of Salivary Proteins From the Argasid Ticks Ornithodoros moubata and Ornithodoros erraticus","volume":"37","author":"Oleaga","year":"2007","journal-title":"Insect Biochem. Mol. Biol."},{"key":"B55","doi-asserted-by":"publisher","first-page":"553","DOI":"10.1186\/s13071-017-2483-z","article-title":"Prevalence of Bartonella Spp. By Culture, PCR and Serology, in Veterinary Personnel From Spain","volume":"10","author":"Oteo","year":"2017","journal-title":"Parasitol. Vectors"},{"key":"B56","doi-asserted-by":"publisher","first-page":"18928","DOI":"10.1073\/pnas.0606379103","article-title":"Quantitative Proteomic Analysis of Distinct Mammalian Mediator Complexes Using Normalized Spectral Abundance Factors","volume":"103","author":"Paoletti","year":"2006","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"B57","doi-asserted-by":"publisher","first-page":"86","DOI":"10.1016\/j.ejbt.2020.09.006","article-title":"Comparison of Protein Precipitation Methods for Sample Preparation Prior to Proteomic Analysis of Chinese Hamster Ovary Cell Homogenates","volume":"48","author":"P\u00e9rez-Rodriguez","year":"2020","journal-title":"Electron. J. Biotechnol."},{"key":"B58","doi-asserted-by":"crossref","DOI":"10.1007\/978-3-642-84414-0","volume-title":"Peritrophic Matrixs","author":"Peters","year":"1992"},{"key":"B59","doi-asserted-by":"publisher","first-page":"14909","DOI":"10.1073\/pnas.1007075107","article-title":"Mechanisms of Suspended Animation are Revealed by Transcript Profiling of Diapause in the Flesh Fly","volume":"107","author":"Ragland","year":"2010","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"B60","doi-asserted-by":"publisher","first-page":"3948","DOI":"10.1242\/jeb.061085","article-title":"Developmental Trajectories of Gene Expression Reveal Candidates for Diapause Termination: A Key Life-History Transition in the Apple Maggot Fly Rhagoletis pomonella","volume":"214","author":"Ragland","year":"2011","journal-title":"J. Exp. Biol."},{"key":"B61","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0044612","article-title":"An Insight Into the Sialotranscriptome of the Cat Flea, Ctenocephalides felis","volume":"7","author":"Ribeiro","year":"2012","journal-title":"PloS One"},{"key":"B62","doi-asserted-by":"publisher","first-page":"279","DOI":"10.1111\/j.1365-2583.2008.00800.x","article-title":"Molecular Cloning, Partial Genomic Structure and Functional Characterization of Succinic Semialdehyde Dehydrogenase Genes From the Parasitic Insects Lucilia cuprina and Ctenocephalides felis","volume":"17","author":"Rothacker","year":"2008","journal-title":"Insect Mol. Biol."},{"key":"B63","doi-asserted-by":"publisher","first-page":"32","DOI":"10.1016\/j.molbiopara.2008.06.001","article-title":"Succinic Semialdehyde Dehydrogenase From the Parasitic Cattle Tick Rhipicephalus microplus: Gene Identification, Biochemical Characterization and Comparison With the Mouse Ortholog","volume":"161","author":"Rothacker","year":"2008","journal-title":"Mol. Biochem. Parasitol."},{"key":"B64","doi-asserted-by":"publisher","first-page":"118","DOI":"10.3390\/insects8040118","article-title":"The Biology and Ecology of Cat Fleas and Advancements in Their Pest Management: A Review","volume":"8","author":"Rust","year":"2017","journal-title":"Insects"},{"key":"B65","doi-asserted-by":"publisher","first-page":"312","DOI":"10.1379\/csc-128r1.1","article-title":"Full Genome Gene Expression Analysis of the Heat Stress Response, in Drosophila melanogaster","volume":"10","author":"S\u00f8rensen","year":"2005","journal-title":"Cell Stress Chaperones"},{"key":"B66","doi-asserted-by":"publisher","first-page":"1055","DOI":"10.1016\/s0965-1748(02)00043-7","article-title":"Cultured Aedes albopictus Mosquito Cells Accumulate Elongation Factor-1 Alpha (EF-1 Alpha) During Serum Starvation","volume":"32","author":"Schwientek","year":"2002","journal-title":"Insect Biochem. Mol. Biol."},{"key":"B67","doi-asserted-by":"publisher","first-page":"31","DOI":"10.1177\/1098612X12470341","article-title":"Flea Control in Cats: New Concepts and the Current Armoury","volume":"15","author":"Siak","year":"2013","journal-title":"J. Feline Med. Surg."},{"key":"B68","doi-asserted-by":"publisher","first-page":"1814","DOI":"10.1007\/s13361-015-1201-3","article-title":"The Sequest Family Tree","volume":"26","author":"Tabb","year":"2015","journal-title":"J. Am. Soc Mass Spectrom."},{"key":"B69","doi-asserted-by":"publisher","first-page":"189","DOI":"10.1007\/s00360-012-0707-2","article-title":"Expression of Genes Involved in Energy Mobilization and Osmoprotectant Synthesis During Thermal and Dehydration Stress in the Antarctic Midge, Belgica antarctica","volume":"183","author":"Teets","year":"2013","journal-title":"J. Comp. Physiol. B."},{"key":"B70","doi-asserted-by":"publisher","first-page":"764","DOI":"10.1152\/physiolgenomics.00042.2012","article-title":"Combined Transcriptomic and Metabolomic Approach Uncovers Molecular Mechanisms of Cold Tolerance in a Temperate Flesh Fly","volume":"44","author":"Teets","year":"2012","journal-title":"Physiol. Genomics"},{"key":"B71","doi-asserted-by":"publisher","first-page":"87","DOI":"10.1016\/s0965-1748(98)00123-4","article-title":"Peritrophic Matrix Proteins","volume":"29","author":"Tellam","year":"1999","journal-title":"Insect Bioch. Mol. Biol."},{"key":"B72","doi-asserted-by":"publisher","first-page":"59","DOI":"10.1186\/1756-3305-6-59","article-title":"Fleas Infesting Pets in the Era of Emerging Extra-Intestinal Nematodes","volume":"6","author":"Traversa","year":"2013","journal-title":"Parasit. Vectors"},{"key":"B73","doi-asserted-by":"publisher","first-page":"731","DOI":"10.1038\/nmeth.3901","article-title":"The Perseus Computational Platform for Comprehensive Analysis of (Prote)Omics Data","volume":"13","author":"Tyanova","year":"2016","journal-title":"Nat. Methods"},{"key":"B74","doi-asserted-by":"publisher","first-page":"S22","DOI":"10.1016\/j.vetpar.2017.02.026","article-title":"Efficacy of a New Spot-on Formulation of Selamectin Plus Sarolaner for Cats Against Adult Ctenocephalides felis, Flea Egg Production and Adult Flea Emergence","volume":"238","author":"Vatta","year":"2017","journal-title":"Vet. Parasitol."},{"key":"B75","doi-asserted-by":"publisher","first-page":"634","DOI":"10.1016\/j.ibmb.2009.07.001","article-title":"Identification and Characterization of Two Arginine Kinases From the Parasitic Insect Ctenocephalides felis","volume":"39","author":"Werr","year":"2009","journal-title":"Insect Biochem. Mol. Biol."},{"key":"B76","doi-asserted-by":"publisher","first-page":"359","DOI":"10.1038\/nmeth.1322","article-title":"Universal Sample Preparation Method for Proteome Analysis","volume":"6","author":"Wi\u015bniewski","year":"2009","journal-title":"Nat. Methods"},{"key":"B77","doi-asserted-by":"publisher","first-page":"1567","DOI":"10.1016\/s0965-1748(02)00077-2","article-title":"Sequences of Elongation Factors-1\u03b1 and -1\u03b3 and Stimulation by Juvenile Hormone in Locusta migratoria","volume":"32","author":"Zhou","year":"2002","journal-title":"Insect Biochem. Mol. Biol."}],"container-title":["Frontiers in Cellular and Infection Microbiology"],"original-title":[],"link":[{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fcimb.2022.828082\/full","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,1,28]],"date-time":"2022-01-28T06:16:21Z","timestamp":1643350581000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fcimb.2022.828082\/full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,1,28]]},"references-count":77,"alternative-id":["10.3389\/fcimb.2022.828082"],"URL":"https:\/\/doi.org\/10.3389\/fcimb.2022.828082","relation":{},"ISSN":["2235-2988"],"issn-type":[{"value":"2235-2988","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,1,28]]},"article-number":"828082"}}