{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T14:45:32Z","timestamp":1787237132621,"version":"build-2736575974"},"reference-count":34,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2022,3,22]],"date-time":"2022-03-22T00:00:00Z","timestamp":1647907200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Cell. Infect. Microbiol."],"abstract":"<jats:p>\n                    <jats:italic>Brucella<\/jats:italic>\n                    , the intracellular bacteria, have evolved subtle strategies to efficiently survive and replicate in macrophages. However, the virulence effector proteins involved are still unclear. LysR-type transcriptional regulators (lttrs) are the largest regulator family with diverse function in prokaryotes. However, very little is known about the role of LysR regulators in the\n                    <jats:italic>Brucella<\/jats:italic>\n                    spp. Here, a\n                    <jats:italic>BSS2_II0858<\/jats:italic>\n                    gene, encoded as one of the LysR-type regulators, was studied. We successfully constructed a\n                    <jats:italic>BSS2_II0858<\/jats:italic>\n                    deletion mutant, \u03940858, and complementation strain C\u03940858 in\n                    <jats:italic>Brucella suis<\/jats:italic>\n                    S2. The cell apoptosis induced by\n                    <jats:italic>B. suis<\/jats:italic>\n                    S2 and its derivatives were detected by flow cytometry. The autophagy was then assessed by immunoblot analysis using the IL3I\/II and p62 makers. In addition, the autophagy flux was evaluated by double fluorescent labeling method for autophagy marker protein LC3. Our studies demonstrated that\n                    <jats:italic>B. suis<\/jats:italic>\n                    S2 and its derivatives inhibited the programmed cell death in early stage and promoted apoptosis in the later stage during infection in RAW264.7 cells. The\n                    <jats:italic>BSS2_II0858<\/jats:italic>\n                    gene was found to play no role during apoptosis according to these results. Compared with the wild-type strain, \u03940858 mutant can stimulate the conversion of LC3-I to LC3-II and markedly inhibited the autophagy flux at early stage leading to obvious autophagosome accumulation. This study explored the function of\n                    <jats:italic>BSS2_II0858<\/jats:italic>\n                    gene and may provide new insights for understanding the mechanisms involved in the survival of\n                    <jats:italic>Brucella<\/jats:italic>\n                    in macrophages.\n                  <\/jats:p>","DOI":"10.3389\/fcimb.2022.858173","type":"journal-article","created":{"date-parts":[[2022,3,27]],"date-time":"2022-03-27T16:00:18Z","timestamp":1648396818000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":8,"title":["A LysR Transcriptional Regulator Manipulates Macrophage Autophagy Flux During Brucella Infection"],"prefix":"10.3389","volume":"12","author":[{"given":"Lu","family":"Zhang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Siyuan","family":"Yu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinnuan","family":"Ning","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui","family":"Fang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jie","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Feijie","family":"Zhi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junmei","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dong","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aihua","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yaping","family":"Jin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2022,3,22]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"523","DOI":"10.1146\/annurev-micro-090110-102905","article-title":"Interactions of the Human Pathogenic Brucella Species With Their Hosts","volume":"65","author":"Atluri","year":"2011","journal-title":"Annu. 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