{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T13:55:27Z","timestamp":1790776527234,"version":"4.1.0"},"reference-count":51,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2022,6,20]],"date-time":"2022-06-20T00:00:00Z","timestamp":1655683200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001665","name":"Agence Nationale de la Recherche","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001665","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002915","name":"Fondation pour la Recherche M\u00e9dicale","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002915","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002428","name":"Austrian Science Fund","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002428","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Cell. Infect. Microbiol."],"abstract":"<jats:p>\n                    The obligate intracellular bacteria\n                    <jats:italic>Chlamydia trachomatis<\/jats:italic>\n                    store glycogen in the lumen of the vacuoles in which they grow. Glycogen catabolism generates glucose-1-phosphate (Glc1P), while the bacteria can take up only glucose-6-phosphate (Glc6P). We tested whether the conversion of Glc1P into Glc6P could be catalyzed by a phosphoglucomutase (PGM) of host or bacterial origin. We found no evidence for the presence of the host PGM in the vacuole. Two\n                    <jats:italic>C. trachomatis<\/jats:italic>\n                    proteins, CT295 and CT815, are potential PGMs. By reconstituting the reaction using purified proteins, and by complementing PGM deficient fibroblasts, we demonstrated that only CT295 displayed robust PGM activity. Intriguingly, we showed that glycogen accumulation in the lumen of the vacuole of a subset of\n                    <jats:italic>Chlamydia<\/jats:italic>\n                    species (\n                    <jats:italic>C. trachomatis<\/jats:italic>\n                    ,\n                    <jats:italic>C. muridarum<\/jats:italic>\n                    ,\n                    <jats:italic>C. suis<\/jats:italic>\n                    ) correlated with the presence, in CT295 orthologs, of a secretion signal recognized by the type three secretion (T3S) machinery of\n                    <jats:italic>Shigella<\/jats:italic>\n                    .\n                    <jats:italic>C. caviae<\/jats:italic>\n                    and\n                    <jats:italic>C. pneumoniae<\/jats:italic>\n                    do not accumulate glycogen, and their CT295 orthologs lack T3S signals. In conclusion, we established that the conversion of Glc1P into Glc6P was accomplished by a bacterial PGM, through the acquisition of a T3S signal in a \u201chousekeeping\u201d protein. Acquisition of this signal likely contributed to shaping glycogen metabolism within\n                    <jats:italic>Chlamydiaceae<\/jats:italic>\n                    .\n                  <\/jats:p>","DOI":"10.3389\/fcimb.2022.866729","type":"journal-article","created":{"date-parts":[[2022,6,20]],"date-time":"2022-06-20T00:49:25Z","timestamp":1655686165000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":5,"title":["CT295 Is Chlamydia trachomatis\u2019 Phosphoglucomutase and a Type 3 Secretion Substrate"],"prefix":"10.3389","volume":"12","author":[{"given":"S\u00e9bastien","family":"Triboulet","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maimouna D.","family":"N\u2019Gadjaga","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B\u00e9atrice","family":"Niragire","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephan","family":"K\u00f6stlbacher","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthias","family":"Horn","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vishukumar","family":"Aimanianda","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Agathe","family":"Subtil","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2022,6,20]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"955","DOI":"10.1016\/j.biocel.2012.03.002","article-title":"Secretion of the Housekeeping Protein Glyceraldehyde-3-Phosphate Dehydrogenase by the LEE-Encoded Type III Secretion System in Enteropathogenic Escherichia Coli","volume":"44","author":"Aguilera","year":"2012","journal-title":"Int. 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